DNA sequencing PCR system.

DNA sequencing PCR system.

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Cyst nematodes are obligate parasitic nematodes found in the fields of many cultivated crops. These nematodes, which have great economic importance, pose a threat to food security, though they are frequently ignored or misdiagnosed as pests because of covert parasitism. A cyst nematode population parasitizing on Artemisia lavandulaefolia DC., one o...

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Context 1
... D2-D3 region of 28S-rDNA was amplified using universal primers D2A (5 ′ -ACAAGTACCGGGGAAAGTTG-3 ′ ) and D3B (5 ′ -TCGGAAGGACAGC-TACTA-3 ′ ) [9]. The PCR reaction conditions for the ITS-rDNA region and the D2-D3 region of 28S-rDNA were consistent, as shown in Table 2: ...

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During the observation of a garlic plantation, a sample consisting of plants with symptoms of growth inhibition and the soil surrounding the roots was collected. Phytophagous nematodes were isolated from the soil sample as well as the garlic plant shoots, with an average density of 75 individuals per 100 mL. Microscope slides were prepared from adu...