Ct values of XopE3 gene amplification of planktonic (a) and biofilm cells (b) of Xap strain CITA 33 at different cellular growth times. P, planktonic cells; B, biofilm; V, alive cells; I, inactivated cells; XX, samples treated with 100 µM PMAxx™

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Viability of the Xap strain CITA 33 in planktonic and biofilm states...

Ct values obtained from v-qPCR using different intercalating agents...

Ct values obtained after v-qPCR from serial dilutions of DNA from alive...

Ct values of XopE3 gene amplification of planktonic (a) and biofilm...

Calibration curve of log (cfu/mL) versus threshold cycle (Ct) after...

A reliable qPCR technique for detecting viable Xanthomonas arboricola pv. pruni cells

Article

Full-text available

Sep 2024

Pilar Sabuquillo
Isabel M. Berruete
Jaime Cubero
Ana Palacio-Bielsa

Xanthomonas arboricola pv. pruni (Xap) is the causal agent of bacterial spot of stone fruits and almond (Prunus spp). Detection of Xap is typically carried out using quantitative real-time PCR (qPCR) combined with culture-based isolation. However, qPCR does not differentiate between viable and dead cells, potentially leading to an overestimation of...