Figure - available from: Applied Microbiology and Biotechnology
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Ct values obtained after v-qPCR from serial dilutions of DNA from alive Xap strain CITA 33 suspensions ranging from 1 to 10⁸ cfu/mL. I, dead cells; XX, samples treated with PMAxx™; V, viable bacteria. The graph shows the means of two different assays, each with two replicates. The “zig-zags” observed at the lowest concentrations are attributable to the non-strict linear correlation between Ct and concentration in these samples (Fig. 5)
Source publication
Xanthomonas arboricola pv. pruni (Xap) is the causal agent of bacterial spot of stone fruits and almond (Prunus spp). Detection of Xap is typically carried out using quantitative real-time PCR (qPCR) combined with culture-based isolation. However, qPCR does not differentiate between viable and dead cells, potentially leading to an overestimation of...
