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... determination of salicin and chlorogenic acid in the leaves, branches, and fruits in V. opulus and V. lantana were carried out by RP-HPLC using the external standard method (Fig. 1). Results of salicin and chlorogenic acid contents in different organs of V. opulus and V. lantana are given in Table 1. ...Similar publications
Zusammenfassung. Synanthedon andrenaeformis (Laspeyres, 1801) ist im Jahr 2009 erstmals aus Sachsen nachgewiesen worden. Die Falter wurden in Hoyerswerda mit synthetischen Pheromonen angelockt. Daraufhin gelang es, die Entwicklung der Art in der Nähe des ersten Fundortes zu bestätigen. Die Nahrungspflanze der Larve ist Viburnum lantana. Die nächste...
The majority of plant colours are produced by anthocyanin and carotenoid pigments, but colouration obtained by nanostructured materials (i.e. structural colours) is increasingly reported in plants. Here, we identify a multilayer photonic structure in the fruits of Lantana strigocamara and compare it with a similar structure in Viburnum tinus fruits...
Manual to implement a pilot study. Developed within the ICP Forests Expert Panel on Ambient Air Quality
A simple and sensitive method for separation and determination of chlorogenic acid has been developed. Chlorogenic acid was separated using a Supelcosil LC 18 column (250 x 4.6 mm, 5 μm) by gradient elution at the flow rate of 1.2 mL/min. The composition of mobile phase consisted of ophosphoric acid, bidistilled water (0.2%) and acetonitrile. Spect...
ABSTRACT
Viburnum mullaha (Buch.-Ham. Ex D. Don), is an underexplored wild edible fruit of Indian
Himalayan region, analysed for total polyphenol contents, antioxidant, anti-elastase, anticollagenase,
anti-tyrosinase activities using in-vitro assays. High values of total phenolic
contents of 1257±40 mg gallic acid equivalents/100 g fruit weight (FW...
Citations
... All assays were performed according to the manufacturer's instructions. The content of salicin and chlorogenic acid were quantified with HPLC (High Performance Liquid Chromatography) 79 . Twenty mg standard salicin or chlorogenic acid (MACKLIN, Shanghai, China) were accurately weighed and revolved into 1 mL 70% methanol. ...
Dietary specialization between insect stages can reduce intraspecific food competition. The involvement of gut bacteria and the mechanisms underlying this phenomenon received limited attention. Plagiodera versicolora is a pest harming Salicaceae trees. Here, we confirmed dietary specialization in P. versicolora, wherein adults prefer new leaves, while larvae predominantly consume mature leaves when both types are available. We demonstrated the larval preference for mature leaves confers ecological advantages by promoting growth, development and immunity and this advantage is contingent upon the presence of gut bacteria. Gut microbiota in larvae revealed a significant enrichment of Pantoea when feeding new leaves, with P. anthophila exhibiting the most pronounced inhibitory effect on larval development. Further exploration identified specific metabolites, such as Tyrosyl-valine, with higher content in new leaves, which serve as substrates for the entomopathogenic gut bacterium to facilitate its proliferation. This study provides a fresh perspective on the ecological role of gut bacteria.
... This observation was further reinforced by Perova et al. [1], who indicated that its contribution to the total amount of phenolics could be up to 96.2%. Altun and Yilmaz [81] found an abundance of chlorogenic acid in the V. opulus fruit, followed by leaves, which supports the findings of the current study. The abundance of chlorogenic acid in the fruit and juice of V. opulus makes this object less attractive due to its remarkable bitterness and astringency [82,83]. ...
In the process of considering the documented health benefits of Viburnum opulus L. (V. opulus), including its anti-inflammatory and antioxidant activities, the present study was designed to qualitatively and quantitatively evaluate the biochemical profile and antimicrobial potency of four commercially available V. opulus extracts. These extracts were obtained from its flowers, bark, berries, and a mixture thereof by cold ultrasound-assisted extraction. An examination of the V. opulus extracts indicated a relative abundance of group compounds, such as phenolics, flavonoids, tannins, and anthocyanins, which are responsible for antioxidant activity (AOA). The widest range in all of the four group compounds was detected in the V. opulus extract sourced from berries, whereas the narrowest range was found in those obtained from flowers. The HPLC-ESI-TQ-MS/MS technique displayed relative fluctuations in the concentrations of individual amino acids (AAs) over the four V. opulus extracts. The prevalence of proline was marked in the flower-derived extract, which made up 63.3% of the total AAs, while aspartic and glutamic acids dominated in the berry-derived extract by contributing up to 29.2 and 24.4% to the total AA content, respectively. Profiling of the individual phenolic compounds disclosed the superiority of chlorogenic acid (up to 90.3%) in the berry and mixed extracts, as well as catechin (up to 57.7%) and neochlorogenic acid (11.1%) in the bark extract, which conveyed a remarkable contribution toward antimicrobial activity. The lowest content of individual phenolics was found in the flower extract. Owing to its substantially denser bioactive composition, the V. opulus berries and bark extracts exhibited markedly better AOA, which was pinpointed by three independent methods, i.e., DPPH•, FRAP, and ABTS•+, than those obtained from flowers or a mixture of V. opulus morphological parts. As part of the antimicrobial activity testing, the V. opulus extracts exhibited outstanding inhibitory activity and a homeopathic mode of action. The V. opulus extracts obtained from a mixture, bark, and berries were more active against 8 out of 19 selected test microorganisms at minimum inhibitory concentration (MIC) values that ranged from 0.24 to 0.49 µL mL−1. Overall, the extracts of V. opulus were found to be effective against Gram-positive and Gram-negative bacteria. However, their conceivable exploitation as functional or pharmaceutical ingredients must be further clarified within in vivo models.
... The Viburnum genus, belonging to the Caprifoliaceae family, includes more than 230 species spread from South America to Southeast Asia, with most being endemic (10). VO, commonly known as the European cranberry bush, has red and oval fruit. ...
Objective: Recent research has centered on the role of oxidative stress and free oxygen radicals in the formation of stones. Viburnum opulus L. (VO) is a fruit species known for its antioxidant activity, and its juice preparation is used in Turkish traditional medicine for stone removal. This study aimed to investigate the effects of VO on calcium oxalate (CaOx) crystallization and oxidative stress in rats with ethylene glycol (EG)-induced nephrolithiasis. Material and Methods: Fifty adult male Wistar Hannover rats were divided into five groups: control (Group 1), EG (Group 2), EG + 50 mg/kg VO (Group 3), EG + 100 mg/kg VO (Group 4), and EG + 200 mg/kg VO (Group 5). On days 7, 14, and 28, 24-hour urine was collected, and blood samples were taken. On day 28, the rats were sacrificed, and inflammation, oxidative stress, and CaOx crystallization in kidney tissue were evaluated under polarized light microscopy. Results: A statistically significant difference was found between Group 1 and Group 2 in terms of serum inflammation parameters, acute kidney injury, and oxidative stress evaluated on days 7, 14, and 28, and tissue inflammation and oxidative stress parameters evaluated on day 28. It was observed that these parameters improved in Groups 3-5 compared to Group 2, and the level of statistical significance increased as the dose increased. In the histopathological evaluation of the tissues on day 28, the mean number of crystals was statistically significantly higher in Group 2 than in Group 1. These parameters improved in Groups 3-5 compared to Group 2, and there was a statistically significant difference when Groups 4 and 5 were compared to Group 2. Conclusion: It was found that VO improved inflammation, oxidative stress, acute kidney injury, and CaOx crystallization in rats with EG-induced nephrolithiasis in direct proportion to the increase in dose.
... The genus Viburnum includes more than 230 species from South America to Southeast Asia, most of them endemic (Altun et al., 2009). In Anatolia, Viburnum species are used for several purposes in folk medicine (Altun and Yilmaz, 2007;Altun et al., 2008). Viburnum species contains diterpenoid, sesquiterpenes, coumarin, phenolic acid, and organic acid, a rich chlorogenic acid source (Altun et al., 2009). ...
Lower urinary tract problems were increasingly encountered in small animal practice. Urinary stones and crystals are common problems in these cases and are still a problem in cats and dogs. Dilution of urine or restriction of mineral intake is a way to reduce urolithiasis/crystalluria. Viburnum Opulus was prescribed as anti-urolitiatic in human medicine. The objective of this study was to evaluate the efficacy of Viburnum Opulus in urolithiasis or crystalluria in companion animals. For this purpose, 42 animals were presented with urinary system problems and diagnosed with urolithiasis or crystalluria, which were included in the study and divided into two main groups (21 calcium oxalate, 21 struvite) and two subgroups (Control and Study). The urinary score was noted as the presence of pollakiuria, crystals in the microscopic examination, hematuria, stranguria, proteinuria, leucocytes, and blood in the urine. The mean ages were 3.81 and 3.95 for the calcium oxalate and struvite groups, respectively. It was determined that the clinical urinary score of the calcium oxalate control group was 11.39 before the treatment and increased to 14.06 at the end of 2 weeks. However, it was observed that this score decreased from 10.71 to 8.71 in the calcium oxalate study group. In calcium oxalate-diagnosed animals, a decrease in clinical scores was significant in the Viburnum opulus group than in controls. The urinary scores decreased in both struvite subgroups, but no statistical differences were determined. All animals except the calcium oxalate control group were clinically healthy at the second visit. Using Viburnum opulus as an integrative medical approach will provide safe and effective treatment for urolithiasis/crystalluria. Keywords: viburnum opulus, urolithiasis, crystalluria, small animal, supplement
... In our study, we did not detect these compounds in the leaves. Just like us, Altun and Yilmaz [56], in V. opulus leaves and branches, showed chlorogenic acid and salicin. In fruits, leaves, sprouts and steams, iridoids have been found, also [12,57,58]. ...
Guelder rose (Viburnum opulus L.) is known for its health benefits. V. opulus contains phenolic compounds (flavonoids and phenolic acids), a group of plant metabolites with wide biological activities. They are good sources of natural antioxidants in human diets owing to their prevention of the oxidative damage responsible for many diseases. In recent years, observations have shown that an increase in temperature can change the quality of plant tissues. So far, little research has addressed the problem of the common impact of temperature and place of occurrence. Towards a better understanding of phenolics concentration that could indicate their potentials as therapeutic agents and towards predicting and controlling the quality of medicinal plants, the aim of this study was to compare phenolic acids and flavonoids content in the leaves of cultivation and wild collection V. opulus, and to examine the impacts of temperature and place of occurrence on their content and composition. Total phenolics were determined using the spectrophotometric method. Phenolic composition of V. opulus was determined using high-performance liquid chromatography (HPLC). The following hydroxybenzoic acids there were identified: gallic, p-hydroxybenzoic, syringic, salicylic, benzoic, as well as hydroxycinnamic acids: chlorogenic, caffeic, p-coumaric, ferulic, o-coumaric and t-cinnamic. The analysis of extracts from V. opulus leaves has indicated the presence of the following flavonoids: flavanols: (+)-catechin and (−)-epicatechin; flavonols: quercetin, rutin, kaempferol, myricetin; and flavones: luteolin, apigenin and chrysin. The dominant phenolic acids were p-coumaric and gallic acids. The major flavonoids found in V. opulus leaves were myricetin and kaempferol. Temperature and plant location affected the concentration of tested phenolic compounds. The present study shows the potential of naturally grown and wild V. opulus for the human.
... Turek and Cisowski [48] using high-pressure liquid chromatography (HPLC) as well as electrospray ionization time-of-flight mass spectrometry (ESI-TOF MS) and proton nuclear magnetic resonance ( 1 H-NMR) methods have reported the presence of chlorogenic acid as the free acid and many acids liberated from esters and glycosides after alkaline and enzymatic hydrolyses such as gallic, 4-hydroxybenzoic, protocatechuic, 3,4-dixydroxyphenylacetic, 3,4,5-trimetoxybenzoic, syringic, homogentisic, caffeic, ferulic, p-coumaric, and ellagic acids. Altun and Yilmaz [63] determined salicin and chlorogenic acid in VO leaves and branches. Dried leaves contained 0.90% of salicin and 0.68% of chlorogenic acid. ...
Viburnum opulus (VO) is a valuable decorative, medicinal, and food plant. This deciduous shrub is found in natural habitats in Europe, Russia, and some regions in North Africa and North Asia. The VO is traditionally used to treat aliments such as cough, colds, tuberculosis, rheumatic aches, ulcers, stomach, and kidney problems, among others. Many of the health-promoting properties of VO are associated with antioxidant activity, which has been demonstrated in both in vitro and in vivo studies. The results of in vitro studies show the antimicrobial potential of VO, especially against Gram-positive bacteria. In cell-based studies, VO demonstrated anti-inflammatory, anti-obesity, anti-diabetic, osteogenic, cardio-protective, and cytoprotective properties. The applicability of VO in the treatment of urinary tract diseases, endometriosis, and some cancers has been confirmed in in vivo studies. The health benefits of VO result from the presence of bioactive components such as phenolic compounds, vitamin C, carotenoids, iridoids, and essential oils. The aim of this review is to present an overview of the botanical characteristics, chemical compositions, including bioactive compounds, and pro-health properties of VO different morphological parts.
... Systemic administration is used in mild asthma, epileptic seizures, hypertension, certain heart conditions, cramps, menstrual pain, mumps, postpartum pain, sleep disorders, and certain conditions of the nervous system, while external use is reserved for skin conditions such as eczema. Another field where it is applied is that of limb or abdominal cramps or other parts in women, especially during pregnancy, and menstrual pain (Felter & Loyd 2004;Velioglu, Ekici, & Poyrazoglu, 2006;Sagdic, Aksoy, & Ozkan, 2006;Altun & Yılmaz, 2007). In vitro studies confirm that the Viburnum species or their extracts have antioxidative and antimicrobial effects (Ersoy et al, 2017;Kraujalytė, Venskutonis, Česonienė, & Daubaras, 2013;Eryılmaz et al., 2013;Česonienė, Daubaras, Kraujalytė, Venskutonis, & Šarkinas, 2014). ...
... Many antioxidants have also been identified as natural mutagens and carcinogens, apart from their role as natural anti-mutagens and anti-carcinogens (Ames 1983;Stavric 1994;Middleton, Kandaswami, & Theoharides, 2000;Franke et al., 2004). A number of recent reports have observed that gilaburu has high level antioxidant activity and antimicrobial potential (Sagdic, Aksoy, & Ozkan, 2006;Altun & Yılmaz, 2007;Ulger et al., 2013). For this reason, in the present study, we evaluate the genotoxic effect of gilaburu juice on HUVEC cells. ...
The purpose of this study is to investigate the effects of gilaburu juice on cell viability (3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl tetrazolium bromide, MTT) and angiogenesis (tube formation assay) using different cell lines (human cancer cell lines A549, Caco-2, HeLa and normal cell lines MDCK and HUVEC) in vitro. In addition, the genotoxic effects of gilaburu juice is evaluated using COMET assay on HUVEC cells. Our results demonstrate that gilaburu juice could inhibit the growth of Caco-2 and HeLa cancer cell lines, but could not significantly inhibit normal cell lines and A549 cancer cell lines. It disrupted tube formation of HUVEC cells. Gilaburu juice appears to have no genotoxic potential to the DNA of HUVEC cells. The results obtained in this study confirm the potential application of commercial gilaburu juice as a functional food in prevention of cancer.
... In the present study, we revealed the MeOH extracts of the fruit and branch parts of V. orientale with a notable cholinesterase inhibitory effect, being more active against BChE at 200 μg/mL (Table 1). Viburnum species are known to contain phenolic acids, chlorogenic acid in particular, as well as glycosidic compounds such as salicin, which we earlier detected in V. opulus and V. lantana by HPLC (Altun and Sever Yilmaz, 2007). On the other hand, one can speculate that salicin may most likely exist in V. orientale. ...
Neurobiological effects of the fruit, leaf, and branch extracts of Viburnum orientale, used in the traditional drink called “gilaburi” in Anatolia, were tested against acetyl- (AChE), butyrylcholinesterase (BChE), and tyrosinase (TYR). Antioxidant effect of the extracts was determined using six in vitro methods. The fruit and branch methanol extracts with the richest total phenolic contents exerted the highest inhibition against all enzymes. These extracts were the most active in DPPH radical scavenging, ferric- (FRAP) and phosphomolibdenum-reducing antioxidant power (PRAP) assays, which was followed by the leaf aqueous extract with the best nitric oxide (75.00 ± 1.22%), DMPD scavenging (33.70 ± 1.13%), and metal-chelating (54.66 ± 3.56%) effects at 2500 μg/mL. We previously reported that the fruits are rich in chlorogenic acid, which might be related to its marked enzyme inhibitory and antioxidant effect. Our results indicated that all methanol and leaf aqueous and branch extracts of the plant are promising for their neuroprotective potential.
... Viburnum opulus L. (Caprifoliaceae family) referred to as "snowball or guelder rose" is grown in many European countries [5,6]. It is worthy to mention that V. opulus (VO) comprise sources of antimicrobial compounds, such as polyacids, phenolic glucosides, anthocyanins, tannin, terpenoide, iridoide, and some organic acids [7][8][9][10]. In addition to that, protein content of VO fruit and seed was determined around 5% [11][12][13]. ...
... In this study, NSBs were formed of VO extract and Cu 2+ ions. It is well known that VO extract contains different types of compounds, such as valeric acid, tannins, iridoid [47], proanthocyanidins [48], arbutin, valerianic acid, flavonoids [49]; chlorogenic acid [7], L-malic acid, ascorbic acid, phenolic compounds [50], catechin, epicatechin, cyanidin-3-glucoside, cyanidin-3-rutinozit, quercetin [51], polysaccharides [52], amino acids and proteins. Among those compounds, molecules containing amine and diols groups may interact with copper phosphate to form NSBs. The formation mechanism of protein-inorganic hybrid snowballs was described before [35,39,40]. ...
... In our previous study, the MeOH extracts of branch, leaf and fruit of V. opulus were analyzed by high-performance liquid chromatography for their chlorogenic acid contents and V. opulus fruits were found to contain the highest chlorogenic acid amounts (1.24%). In the same study, V. lantana fruit were found to have 0.01% chlorogenic acid as the same in V. tinus branch (27). ...
A simple and sensitive method for separation and determination of chlorogenic acid has been developed. Chlorogenic acid was separated using a Supelcosil LC 18 column (250 x 4.6 mm, 5 μm) by gradient elution at the flow rate of 1.2 mL/min. The composition of mobile phase consisted of ophosphoric acid, bidistilled water (0.2%) and acetonitrile. Spectrophotometric detection was carried out at 330 nm. The linear range of detection for chlorogenic acid was between 0.7237-500 μg/mL. The method described was suitable for the determination of chlorogenic acid in the leaves, branches and fruits of Viburnum tinus L. and Viburnum orientale Pallas. It was observed that V.orientale fruit sample has the highest chlorogenic acid content (0.5069%), while V.tinus branches (0.0141%) have the lowest chlorogenic acid content as w/w (%) in our case. This study is the first application of High Performance Liquid Chromatography (HPLC) method to the determination of chlorogenic acid content of V. tinus and V. orientale in Turkey.
