Figure 2 - uploaded by Dessislava Todorova
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Content of free (A), conjugated (B) and bound (C) spermidine in zucchini cotyledons (mean values ±SE). Cotyledons were excised from the embryonic axes of dry Cucurbita pepo L. (zucchini) seeds and placed in covered Petri dishes on filter paper soaked with distilled water supplemented with either MeJA or BA, each applied at a concentration of 0.01 mM. Cotyledons were cultivated in the dark at 25°C. The analyses were performed on 6-day-old cotyledons. N.D. – not detected.
Contexts in source publication
Context 1
... to free Spd, BA provoked a substantial increase of endogenous free Spm (by almost 40%) as compared to control (Fig. 3A). The alterations of this fraction were not significant in MeJA-incubated cotyledons, the amount remaining near to the control value. The application of MeJA alone did not change noticeably conjugated Spm (Fig. 3B). However, BA totally reduced TCA-soluble bound Spm. No significant changes in the content of bound Spm were detected in ...
Context 2
... to free Spd, BA provoked a substantial increase of endogenous free Spm (by almost 40%) as compared to control (Fig. 3A). The alterations of this fraction were not significant in MeJA-incubated cotyledons, the amount remaining near to the control value. The application of MeJA alone did not change noticeably conjugated Spm (Fig. 3B). However, BA totally reduced TCA-soluble bound Spm. No significant changes in the content of bound Spm were detected in MeJA-treated cotyledons whereas the addition of BA into the medium increased bound Spm by 33% (Fig. ...
Context 3
... the amount remaining near to the control value. The application of MeJA alone did not change noticeably conjugated Spm (Fig. 3B). However, BA totally reduced TCA-soluble bound Spm. No significant changes in the content of bound Spm were detected in MeJA-treated cotyledons whereas the addition of BA into the medium increased bound Spm by 33% (Fig. ...