Table 1 - uploaded by Tirthesh Sharma
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Concentration of flavonoids in Vitex negundo extract in per gm dry weight of its stem leaf and root.
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Context 1
... Estimation of Flavonoids:- All the parts (Stem, roots, and leaves) of Vitex negundo showed positive response in the preliminary detection test of flavonoids. Flavonoid contents estimated in each gram of dried plant material was recorded (Table-1). Content of free flavonoids were obtained maximum in leaves (7.50 mg/g.d.w) and minimum in roots (4.20 mg/g.d.w) whereas bound flavonoids was maximum in stem (0.47mg/g.d.w) and minimum in leaves (0.28mg/g.d.w). Total flavonoids yield was observed maximum in leaves (7.78 mg/g.d.w) whereas minimum in roots (4.55mg/g.d.w). ...
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Vitex negundo a tropical shrub from the Western Ghats possess various medicinal applications. This study reports the antioxidant and antibacterial activities of gold nanoparticles synthesized from V. negundo leaf extracts. The V. negundo leaf extracts were prepared by cold maceration process and green synthesis of gold nanoparticles was performed by gold reduction method. The characterization analyses, such as ultraviolet–visible spectroscopy, fourier transform infrared spectroscopy, X-ray diffraction, energy-dispersive X-ray diffraction, scanning electron microscopy and transmission electron microscopy were carried out. The antioxidant activity of the gold nanoparticles were determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) method and nitric oxide assay. The green synthesized nanoparticles were in the size range of 20–70 nm and exhibited notable antioxidant activity. The IC50 values of DPPH and nitric oxide assays were found to be 62.18 µg and 70.45 µg respectively. In addition, antibacterial analysis of six food borne pathogens (E. coli, B. subtilis, P. aeruginosa, S. aureus, S. typhimurium, and S. pyogenes) was studied by well-diffusion method. The gold nanoparticles displayed strong antibacterial activity against gram negative strains and moderate activity against gram positive strains.
