Comparison of methods to identify the etiology of eye disease.

Comparison of methods to identify the etiology of eye disease.

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Ocular diseases affect multiple eye parts and can be caused by pathogenic infections, complications of systemic diseases, genetics, environment, and old age. Understanding the etiology and pathogenesis of eye diseases and improving their diagnosis and treatment are critical for preventing any adverse consequences of these diseases. Recently, the ad...

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Context 1
... culture techniques can successfully isolate various microorganisms, such as bacteria and fungi (Table 1), and positive culture results can help make a clear etiological diagnosis of the corresponding disease. However, the culture technique can only isolate specific microorganisms that meet the conditions of the Petri dish, which fails to show the overall composition of the microbial community (Table 3). Moreover, culture techniques typically require several weeks or more to provide conclusive results, and the rate of positive results is low [15], requiring the aid of other pathogen detection methods. ...
Context 2
... in PCR experiments, only predetermined sequences can be distinguished, resulting in many potential pathogens not being identified [27,29]. The final PCR results vary due to the different sources of samples, and there may be room for further improvement in its sensitivity (Table 3). ...
Context 3
... to efficiently utilize HTS in ophthalmology, many of its limitations need to be resolved (Table 3). MDS can theoretically detect all pathogens in clinical samples. ...