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Comparison of NPV results obtained for AGID and IAC-PCR. The dashed red line indicates predicted NPV for AGID; the blue line indicates estimated NPV for IAC-PCR
Source publication
Introduction
The aim of the study was to estimate the diagnostic sensitivity (DSe) and specificity (DSp) of an agar gel immunodiffusion (AGID) assay for detection of myxoma virus (MYXV) in the classical form of myxomatosis and to compare its diagnostic performance to that of molecular methods (IAC-PCR, OIE PCR, and OIE real-time PCR).
Material and...
Contexts in source publication
Context 1
... finding could explain why AGID is still included in the panel of OIE methods used for disease detection. It is noteworthy that AGID is also widely employed in the diagnosis of other viral diseases of animals mainly as a screening tool (1, 8, 9). It appeared to be a sensitive assay in detection of Marek's disease virus (MDV) in clinically affected and dead birds from disease outbreak flocks, in the testing of which no significant difference between PCR and AGID in the detection of MDV was observed (8). ...
Context 2
... number of true positive samples tested in AGID, especially those with levels of MYXV lower than 3,125 PCRU, the percentage of false negative results will also increase. For example, assuming that 40% of tested samples will contain the virus (the probability of infection), the probability of getting a false negative result using AGID will be 20% (Fig. 1). Using the IAC-PCR method to test these samples, no false negative results will be obtained, and all virus positive samples will be correctly identified. Due to a lack of samples originating from rabbits with the atypical (amyxomatous) form of the disease, it was not possible to assess the diagnostic value of AGID for rabbit tissues ...
Citations
Myxomatosis is an acute viral disease of rabbits caused by Myxoma virus, poxvirus belonging to the genus Leporipoxvirus. The natural hosts of this pathogen are tapeti (Sylvilagus brasiliensis), and brush rabbits (Sylvilagus bachmani) in in South and Central America, as well as in North America, respectively. The virus of myxoma develops only a mild disease in those species, but in European rabbits (Oryctolagus cuniculus) occurred the fatal disease.The disease is endemic in many European, Asian and American countries, but the agent is still recognized as an emergent infection, associated with mass losses and high mortality in European rabbits.One Health Scientific and Research Institute, PSI in collaboration with SRI ‘Veterinary Biotechnologies’, LLC were developed the methodical approach for detection of the DNA of MYXV using Real-Time PCR due the reason of enhancement of myxomatosis control measures in Ukraine.
