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Cell line models used to study EMT in cSCC (used in > 5% of studies)

Cell line models used to study EMT in cSCC (used in > 5% of studies)

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Cutaneous squamous cell carcinoma (cSCC) is a disease with globally rising incidence and poor prognosis for patients with advanced or metastatic disease. Epithelial-mesenchymal transition (EMT) is a driver of metastasis in many carcinomas, and cSCC is no exception. We aimed to provide a systematic overview of the clinical and experimental evidence...

Contexts in source publication

Context 1
... excluded covered either a different disease (melanoma, BCC, cervical SCC, HNSCC), were secondary literature (review articles, book chapters), were unrelated to EMT, and/or used inducible murine models (DMBA, TPA, UV). Eligible studies encompassed 44 cell line-based (experimental) studies, 22 clinical studies, and 20 studies integrating both cell line work and clinical investigation (Supplementary Table 1 and 2). ...
Context 2
... addition, integration of EMT-TFs and linking of specific transcription factors to their influences on EMT markers and properties in keratinocytes is infrequent. While many of the experimental studies met TEMTIA guidelines, the cell line models used to investigate EMT in cSCC (Table 1) misrepresent the patient population and aetiology. The most frequently used model (40%) is the vulva-derived A431 cell line. ...
Context 3
... were no uniform set of markers used to assess EMT. A listing for each study specifying marker and methods is available in Supplementary Table 1 and 2. The most frequently employed epithelial and mesenchymal markers were E-cadherin, Cytokeratins, and EpCAM as well as Vimentin, N-Cadherin, Fibronectin and α-SMA actin, respectively. Sometimes other non-canonical EMT markers were employed to assess other closely linked properties or processes such as disassembly of cellular junctions (Girdin, α-/βcatenin, ZO1, Desmoglein 3), differentiation and stemness (KLF4, involucrin, CD133, CD44), and ECM remodelling (MMPs, uPAR, PAI-1). ...
Context 4
... EMT was an observation that Ji et al. [15] should repeat. Since then TGF-β has been shown to contribute Table 1 and 2). The signalling investigated by cell line-based studies was merged in this EMT pathway map under the assumption of transferability between the different aetiologies and models. ...