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Capital cost distributions of three scenarios with an annual plant capacity of 50,000 tons. Scenario #1: isobutanol production from biogas; scenario #2: isobutanol production from natural gas; scenario #3: isobutanol production from CO2. Necessary capital cost refers to the sum cost of warehouse, site development, additional piping and land
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Background
The dramatic increase in greenhouse gas (GHG) emissions, which causes serious global environmental issues and severe climate changes, has become a global problem of concern in recent decades. Currently, native and/or non-native C1-utilizing microbes have been modified to be able to effectively convert C1-gases (biogas, natural gas, and C...
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Citations
... Furthermore, although thermodynamic properties are correctly modeled by equations of state such as NRTL and HOC, these predictions are applicable once the product is obtained. Conversely, Liang et al. [53], mention that biomass generation (in this case cyanobacteria), is specified by means of an empirical formula leading to limited predictions in the growth of the microorganism. Figure 4 shows the flow diagram of the process for valorizing CO 2 through bioconversion to ethanol. ...
Carbon dioxide (CO2) emissions have a significant impact on climate change and global warming, with concentrations exceeding the value established as a planetary limit (350 ppm CO2). In Colombia, the manufacturing industries and the final consumption in households contribute to the highest emissions of CO2 to the atmosphere. Sucre region, known for basing its economy on livestock and social services, is responsible for an annual emission of more than 3 Mton the CO2 eq. Then, the state of novelty of this study is the applicability evaluation of methanol and ethanol production technologies based on CCU systems, in terms of techno-economic indicators, to be implemented in Sucre. Technical and economic assessment of the CO2 valorization technologies towards methanol and ethanol production was carried out for a base case corresponding to a CO2 inlet flow corresponding to 10% of the net CO2 emissions in the region (i.e., 1750 kgCO2/h). The results for methanol production through CO2 hydrogenation presented a yield of 59.35% (kgmethanol/kgCO2), a CO2,out/CO2,in ratio of 0.35, a profit margin of 51.07%, and a NPV of 33.42 M.USD. Moreover, the analysis of ethanol production by a biotechnological route to convert CO2 using cyanobacteria (specifically S. elongatus sp. PCC 7942), presented a product yield of 17.61% (kgethanol/kgCO2), a CO2,out/CO2,in ratio of 1.49 × 10–4, a profit margin of 3.86%, and a NPV of − 71.28 M.USD. As conclusions, methanol production is the most viable option to be implemented in the Sucre region at all raw material scales considered. Furthermore, future studies should consider the impact of carbon credits within economic feasibility.
Graphical Abstract
... With recent advancements in synthetic biology, methanotrophs have been used for successful production of variety of biochemicals and demonstration of proof-of-concept studies with highly selective methane bioconversion routes to many specialty chemicals as well. A recent review has discussed a list of biochemicals that can be produced such as polyhydroxyalkanoates, methanol, single cell protein, ectoine, fatty acids, lipids and organic acids (Gęsicka et al., 2021), and 90% utilization of gaseous substrate including methane for isobutanol production (Liang et al., 2022) using methanotrophs as host organisms. ...
Methanotrophic bacteria are promising hosts for methane bioconversion to biochemicals or bioproducts. However, due to limitations associated with long genetic manipulation timelines and, lack of choice in genetic tools required for strain engineering, methanotrophs are currently not employed for bioconversion technologies. In this study, a rapid and reproducible electroporation protocol is developed for type 1 methanotroph, Methylotuvimicrobium alcaliphilum using common laboratory solutions, analyzing optimal electroshock voltages and post-shock cell recovery time. Successful reproducibility of the developed method was achieved when different replicative plasmids were assessed on lab adapted vs. wild-type M. alcaliphilum strains (DASS vs. DSM19304). Overall, a~3-fold decrease in time is reported with use of electroporation protocol developed here, compared to conjugation, which is the traditionally employed approach. Additionally, an inducible (3-methyl benzoate) and a constitutive (sucrose phosphate synthase) promoter is characterized for their strength in driving gene expression.
... In addition to technical considerations, the economic viability and carbon footprints of enzymatic processes in biocatalysis must be carefully evaluated, which is essential for determining their feasibility and sustainability. Techno-economic assessment (TEA) is a valuable tool for assessing the commercial viability and potential improvements of enzymatic techniques, while life cycle assessment (LCA) is commonly used to estimate the environmental impacts of these processes (Fei et al., 2020;Liang et al., 2022). The integration of TEA and LCA has been successfully applied to analyze the relationships between technology, economy, and environment in the bioconversion process of CO2 and CH4 (Fu et al., 2023). ...
... The feasibility for this is supported by, for example, Clostridium autoethanogenum that has been shown to convert CO 2 from biogas, along with renewable H 2 , into ethanol and lactate [72]. Additionally, phototrophic cyanobacteria can convert CO 2 into products, including isobutanol [73] or 3-hydroxypropionic acid [74]. ...
Biomass is widely identified as a promising, renewable replacement for fossil feedstocks in the production of energy, fuels, and chemicals. However, the sustainable supply of biomass is limited. Economic and ecological criteria support prioritization of biomass as a carbon source for organic chemicals; however, utilization for energy currently dominates. Therefore, to optimize the use of available biomass feedstock, biorefining development must focus on high carbon efficiencies and enabling the conversion of all biomass fractions, including lignin and fermentation-derived CO2. Additionally, novel technological platforms should allow the incorporation of nontraditional, currently underutilized carbon feedstocks (e.g. manure) into biorefining processes. To this end, funneling of waste feedstocks to a single product (e.g. methane) and subsequent conversion to chemicals is a promising approach.