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Blastocyst cell number at 168 h after IVF with frozen-thawed boar spermatozoa in the magnetized freezing extender treated with 0 G (n = 14), 2000 G (n = 16), 4000 G (n =15) and 6000 G (n = 17), representative images of stained by Hoechst 33342 (A) and number of cell per blastocyst (B), a,b p < 0.05.
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Understanding freezing is essential for stable cryopreservation of sperm. In the freezing process, ice crystal is developed from a change of matter from liquid water to solid water. Unfortunately, they tend to make damage of membrane in frozen sperm. From a perspective, sperm membrane damage due to ice crystal formation, magnetized f...
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