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Associations between relative microRNA expressions and markers of inflammation, endothelial function and vascular relaxation.
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Chronic inflammation causes dysregulated expression of microRNAs. Aberrant microRNA expression is associated with endothelial dysfunction. In this study we determined whether TNF-α inhibition impacted the expression of miRNA-146a-5p and miRNA-155-5p, and whether changes in the expression of these miRNAs were related to inflammation-induced changes...
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Context 1
... unadjusted regression analysis, the relative expression of miRNA-146a-5p was associated with increased circulating CRP and TNF-α concentrations (both p = 0.01; Table 2). When adjusting for body mass and blood pressure, the association between miRNA-146a-5p and circulating CRP and TNF-α concentrations remained significant (p = 0.009 and p = 0.01, respectively; Table 2). ...Context 2
... unadjusted regression analysis, the relative expression of miRNA-146a-5p was associated with increased circulating CRP and TNF-α concentrations (both p = 0.01; Table 2). When adjusting for body mass and blood pressure, the association between miRNA-146a-5p and circulating CRP and TNF-α concentrations remained significant (p = 0.009 and p = 0.01, respectively; Table 2). Increased miRNA-146a-5p expression was associated with increased circulating PTX-3 concentrations (p = 0.03), but not with circulating VCAM-1 concentrations (p = 0.26; Table 2). ...Context 3
... adjusting for body mass and blood pressure, the association between miRNA-146a-5p and circulating CRP and TNF-α concentrations remained significant (p = 0.009 and p = 0.01, respectively; Table 2). Increased miRNA-146a-5p expression was associated with increased circulating PTX-3 concentrations (p = 0.03), but not with circulating VCAM-1 concentrations (p = 0.26; Table 2). When adjusting for body mass and blood pressure, miRNA-146a-5p remained significantly associated with PTX-3 (std β (SE) = 0.48 (0.16); p = 0.01). ...Context 4
... = 0.02). In unadjusted regression analysis, the relative expression of miRNA-146a-5p was positively associated with mesenteric artery ACh-induced sensitivity (logEC 50 ) (p = 0.04) and this association remained significant in multivariate regression analyses (p = 0.04; Table 2). ...Context 5
... relative expression of miRNA-155-5p was associated with increased circulating CRP concentrations in univariate (p = 0.03) and in multivariate regression models (p = 0.003; Table 2). No significant associations were observed between the relative expression of miRNA- 155-5p and circulating VCAM-1 or PTX-3 concentrations (both p>0.05). ...Context 6
... significant associations were observed between the relative expression of miRNA- 155-5p and circulating VCAM-1 or PTX-3 concentrations (both p>0.05). The relative expression of miRNA-155-5p was negatively associated with mesenteric artery ACh-induced maximum relaxation responses in unadjusted analysis (p = 0.009) and when adjusting for body mass and blood pressure (p = 0.0009; Table 2). When further including CRP concentrations in the regression model, the association between miRNA-155-5p and maximal mesenteric artery relaxation was no longer significant (std β (SE) = -0.32 ...Citations
... This can contribute to cardiovascular pathology by promoting endothelial dysfunction, vascular inflammation, and atherogenesis (Fig. 3). In experimental models, it has been demonstrated that inhibition of TNF-α prevents specific miRNA upregulation and subsequently improves vasorelaxation, hinting at the vast therapeutic potential of targeting miRNA pathways [84]. Additionally, high-intensity interval training has been shown to induce changes in the EG and associated miRNAs, which may serve as a tool for monitoring early vasculoprotective adaptations to physical activity [85]. ...
This narrative review provides a comprehensive examination of the complex interplay between inflammatory arthritis (IA) and cardiovascular pathology. It particularly illuminates the roles of atherosclerosis initiation, endothelial dysfunction, and glycocalyx shedding. IA not only provokes tissue-specific inflammatory responses, but also engenders a considerable degree of non-specific systemic inflammation. This review underscores the accelerating influence of the chronic inflammatory milieu of IA on cardiovascular disease (CVD) progression. A focal point of our exploration is the critical function of the endothelial glycocalyx (EG) in this acceleration process, which possibly characterizes the earliest phases of atherosclerosis. We delve into the influence of inflammatory mediators on microtubule dynamics, EG modulation, immune cell migration and activation, and lipid dysregulation. We also illuminate the impact of microparticles and microRNA on endothelial function. Further, we elucidate the role of systemic inflammation and sheddases in EG degradation, the repercussions of complement activation, and the essential role of syndecans in preserving EG integrity. Our review provides insight into the complex and dynamic interface between systemic circulation and the endothelium.
... For example, studies have shown that colchicine, TNF-alpha inhibitors, and cyclosporin A affect the serum levels of miRNAs in BD patients. [37][38][39][40] Although the medical treatments were not statistically different between BD groups, the serum levels of miR-195 were significantly different in BD patients with vascular involvement. Therefore, this result may indicate a link between vascular involvement and miR-195. ...
Objective: MicroRNAs (miRNAs) are involved in a range of pathological and biological processes. Vascular involvement is an important complication associated with morbidity and mortality in Behçet's disease (BD). In this study, we aimed to evaluate the expression levels of miR-195, miR-424, miR-10b, miR-103a-3p, and miR-542-3p in Turkish patients with BD, and their possible association with vascular involvement and clinical activity. Methods: This cross-sectional study included 61 BD patients and 25 age-and sex-matched healthy individuals. The patients were categorised into two groups based on the presence or absence of vascular involvement. Demographic data, disease duration , disease activity, and medical treatments were recorded. Disease activity was evaluated using the Behçet's Disease Current Activity Form (BDCAF) and the Behçet's Syndrome Activity Scale (BSAS). The expression levels of miRNAs were measured using real-time quantitative polymerase chain reaction (RT-qPCR). Results: The comparison of the clinical features of BD patients with and without vascular involvement revealed no significant difference. However, the expression levels of miR-195, miR-424, miR-10b, miR-103a-3p, and miR-542-3p were significantly higher in BD patients than in healthy controls (p<0.001, p<0.001, p=0.010, p<0.01, p=0.039, respectively). Moreover , the expression level of miR-195 was significantly higher in vasculo-Behçet patients than in the other groups (p=0.0318). However, no significant association was found between the expression levels of miR-195 and clinical activity. Conclusion: Our study results indicated elevated serum levels of miR-195 in BD patients, which may be associated with vascu-lar involvement. Therefore, miR-195 could potentially serve as a biomarker for the diagnosis and monitoring of vasculo-Behçet's disease.
