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-Assessment of FFPE Extracted DNA with Different Factors.

-Assessment of FFPE Extracted DNA with Different Factors.

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-Assessment of FFPE Extracted DNA with Different Factors.
EFFICIENT AND COST-EFFECTIVE METHOD OF HIGH QUALITY DNA EXTRACTION FOR BISULFITE CONVERSION BASED METHYLATION ANALYSIS OF FORMALIN-FIXED PARAFFIN- EMBEDDED ARCHIVAL BREAST TISSUES
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  • Jun 2020
Robust standardized methods to extract DNA from formalin-fixed paraffin-embedded tissues are required for accurate bisulfite conversion based methylation analysis.Despite many different protocols are published, extraction of sufficient quantity of intact DNA from formalin-fixed paraffin-embedded (FFPE) tissues is still challenging. We compared two...
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... of extracted DNA was measured before and after bisulfite conversion. Table 1 shows the assessment of extracted DNA with different factors. In the recently published paper on methylation analysis, the authors suggested the use of Qiagen minikit for good quality DNA extraction from FFPE tissues but due to DNA degradation, yield quality was less than fresh frozen tissues (Ludgate et.al., 2017). ...