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To investigate the in vitro antimicrobial effects of carbamide peroxide (CP) and CP-based home bleaching agents against polymicrobial (PM) biofilms.
Using a high-throughput active attachment model, PM biofilms were cultured on glass coverslips by diluting the stimulated saliva of one healthy adult. All experiments were performed anaerobically in Mc...
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Context 1
... were evaluated using dedicated software (Simple PCI n ). Table 2 shows the antimicrobial effects of home bleaching agents on 24 and 72-hour biofilms. At both times, there were no significant differences between DW and 0.2% NaF, whereas significantly fewer colonies were present after incubation with 0.5% HS, 0.5% OR, and 0.2% CHX. ...
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Citations
... Some previous studies have shown that whitening agents containing HP have bactericidal properties. 49,50 Langsten et al. reported that high-concentration carbamide peroxide bleaching gels (10% and 35% CP for 14 days) did not cause a significant change in the surface roughness of hybrid and micro-filled composites 51 . On the contrary, Basting et al. reported that 10% CP application for 3 weeks increased the surface roughness of condensable composite resins, but the change in surface microhardness was statistically insignificant. ...
EFFECT OF HIGH CONCENTRATION PEROXIDE BLEACHING AGENTS ON THE SURFACE PROPERTIES OF COMPOSITE RESTORATIONS
... Therefore, CP has the properties of both hydrogen peroxide and urea (26). At low doses, it decomposes to produce oxygen at a lower toxicity, while at high doses, it has strong oxidation effects by producing reactive oxygen species (ROS) (27,28). Here, we report the chemical synthesis of CP with different additives through a one-step reaction at room temperature and describe in detail the subsequently derived CP compounds. ...
Background:
Under hypoxic conditions, the brain can undergo irreversible damage. The present study aimed to explore new higher-oxygen-content carbamide peroxide (CP) compounds and the effect of their oxygen-releasing property on human brain microvascular endothelial cell (EC) proliferation under in vitro hypoxic conditions.
Methods:
Two different additives including alpha-terpineol and sorbic acid were added to the reaction system to obtain the carbamide peroxide of CP-I and CP-II.
Results:
We evaluated the oxygen generation capabilities of CP samples by using a portable dissolved oxygen meter. Consequently, alpha-terpineol as a stabilizer exhibited a unique effect on the oxygen generation of CP. CP-I was uniquely able to promote cell proliferation ability at 10 µg·L-1 for hypoxic conditions, with the proliferation rates being 36.2% compared with the control group. The safety of CP to cells was further verified by calcein-AM/PI staining. Under hypoxic conditions, CP-I at 10 µg·L-1 promoted the migration rate, and the migration rate being 32.37%.
Conclusions:
These compounds have the advantages of simple synthesis, long storage time, low cost, and rich oxygen content. Used spectrophotometry, oxygen electrode test, and indicator titration for testing the oxygen production rate and oxygen production. The results indicate that alpha-terpineol is the best additive. CP-I exhibited the highest oxygen content and a superior effect on the cell phenotype than CP-II, especially under hypoxia. This study is the first to report the effects of CP on cells, and provides new therapeutic insights into cerebrovascular injury repair.
... Some previous studies have shown that HP-based bleaching agents have bactericidal properties. [24][25][26] Many factors influence biofilm formation, including surface roughness, surface free energy, and the chemical combination of the surface. It has been shown that increasing the surface free energy increases the bacterial adhesion. ...
Background: Knowledge about the effect of bleaching on behavior of composite resins is important to find a suitable composite resin for restoration of teeth undergoing bleaching. This study aimed to assess the effect of different bleaching protocols on surface roughness and biofilm formation on a silorane-based composite resin. Materials and Methods: In this in vitro experimental study, 60 silorane-based composite resin samples measuring 3 mm in thickness and 6 mm in diameter were fabricated and polished. They were then randomly divided into four groups (n = 15). In Group 1, samples were stored in distilled water as control. Samples in Groups 2, 3, and 4 were subjected to bleaching with 15% carbamide peroxide, 35% hydrogen peroxide, and 35% hydrogen peroxide activated by light, respectively. Surface roughness was measured using a profilometer. Streptococcus mutans cultured in brain-heart infusion broth was used for the assessment of biofilm formation on the samples. The bacterial colonies were counted using the pure-plate technique. Data were analyzed using one-way ANOVA and post hoc Tukey's tests. Regression model was used to assess the association between surface roughness and biofilm formation (P < 0.05). Results: The mean surface roughness of the four groups was not significantly different (P = 0.11); however, a significant difference was noted in the mean biofilm formation among the groups (P = 0.00). Conclusion: Bleaching decreased biofilm formation. The lowest biofilm formation was noted in the group subjected to light-activated 35% hydrogen peroxide. Increased surface roughness enhanced biofilm formation to a certain level; excessive roughness did not increase biofilm formation.
... Some previous studies have shown that HP-based bleaching agents have bactericidal properties. [24][25][26] Many factors influence biofilm formation, including surface roughness, surface free energy, and the chemical combination of the surface. It has been shown that increasing the surface free energy increases the bacterial adhesion. ...
Background: Knowledge about the effect of bleaching on behavior of composite resins is important to find a suitable composite resin for restoration of teeth undergoing bleaching. This study aimed to assess the effect of different bleaching protocols on surface roughness and biofilm formation on a silorane-based composite resin.
Materials and Methods: In this in vitro experimental study, 60 silorane-based composite resin samples measuring 3 mm in thickness and 6 mm in diameter were fabricated and polished. They were then randomly divided into four groups (n = 15). In Group 1, samples were stored in distilled water as control. Samples in Groups 2, 3, and 4 were subjected to bleaching with 15% carbamide peroxide, 35% hydrogen peroxide, and 35% hydrogen peroxide activated by light, respectively. Surface roughness was measured using a profilometer. Streptococcus mutans cultured in brain-heart infusion broth was used for the assessment of biofilm formation on the samples. The bacterial colonies were counted using the pure-plate technique. Data were analyzed using one-way ANOVA and post hoc Tukey's tests. Regression model was used to assess the association between surface roughness and biofilm formation (P < 0.05).
Results: The mean surface roughness of the four groups was not significantly different (P = 0.11); however, a significant difference was noted in the mean biofilm formation among the groups (P = 0.00).
Conclusion: Bleaching decreased biofilm formation. The lowest biofilm formation was noted in the group subjected to light-activated 35% hydrogen peroxide. Increased surface roughness enhanced biofilm formation to a certain level; excessive roughness did not increase biofilm formation.
... In this study, polymicrobial (PM) biofilm modeldeveloped by Exterkate et al. (2010) was used. The purpose of this study was to evaluate the adherence and detachment of biofilms formed on glass surfacesmodified with 10F2S-3I and newly developed heptadecafluorodecylhydroxyethylmethacrylate (FF01) using PM biofilm model (Exterkate et al., 2010;Macbain, 2009;Exterkate et al., 2014;Tomiyama et al., 2015;Hasegawa et al., 2015). ...
The purpose of this study was to evaluate the adherence and detachment of bacterial biofilms formed on glass surface modified with 10F2S unmodified cover glasses were imme and theywere cultured to develop PM biofilms. There were separated six experimental groups (n = 6), designated WC: cover glass washing withCPW, WI: modified with 10F2S with CPW, WF: modified with FF01 cover glass washing with CPW, NC: cover glass without washing, NI: modified with 10F2S glass without washing.The modified and unmodified groups were further divided b washing withCPW or without washing. which were then cultured on blood agar medium to count CFU.PM biofilms were easily detached from 10F2S difference of CFU among NC, NI and NF. But WI and WF were significantly lower than in WC. It was concluded that PM biofilms grown on the glass surface modified were easily detached compared with the unmodifiedglass groups Copyright©2017, Ayako Teranaka et al. This is an open access article distributed under the Creative Commons Att use, distribution, and reproduction in any medium, provided the original work is properly cited.
... In the present study, we adopted the PM biofilm model (16) described by Exterkate et al., which generates biofilms from various bacteria in the oral cavity. This model also generates and treats multiple independent biofilms in single experiments, offers strong adhesion, simultaneously treats different solid phases (substrates), and facilitates easy adjustments of treatment times and drug concentrations (17,(24)(25)(26)(27). No alternative existing biofilm model satisfies all these conditions. ...
We used a polymicrobial (PM) biofilm model to examine associations of bacterial adhesiveness with surface characteristics of various dental materials. Four types of dental materials (apatite pellet, zirconia, ceramic, and composite resin) with rough and mirror surfaces were used. Surface roughness, surface free energy, zeta potential, and colony-forming units (CFUs) of the biofilm formations were measured. Biofilms were cultured for 24 h under anaerobic conditions, plated onto blood agar medium, and anaerobically cultured for 4 days. After culturing, CFU per mm² was calculated, and samples were observed under a scanning electron microscope. Means and standard deviations of the experimental data were estimated, and one-way ANOVA and Tukey multiple comparison assays were performed. Pearson correlation coefficients were obtained for the CFU and surface characteristics. Surface roughness and surface free energy appeared to affect generation of PM biofilms on oral materials, and zeta potential was involved in generation of PM biofilms on mirror-ground oral materials.
... In this study, polymicrobial (PM) biofilm modeldeveloped by Exterkate et al. (2010) was used. The purpose of this study was to evaluate the adherence and detachment of biofilms formed on glass surfacesmodified with 10F2S-3I and newly developed heptadecafluorodecylhydroxyethylmethacrylate (FF01) using PM biofilm model (Exterkate et al., 2010;Macbain, 2009;Exterkate et al., 2014;Tomiyama et al., 2015;Hasegawa et al., 2015). ...
The purpose of this study was to evaluate the adherence and detachment of bacterial biofilms formed on glass surface modified with 10F2S unmodified cover glasses were imme and theywere cultured to develop PM biofilms. There were separated six experimental groups (n = 6), designated WC: cover glass washing withCPW, WI: modified with 10F2S with CPW, WF: modified with FF01 cover glass washing with CPW, NC: cover glass without washing, NI: modified with 10F2S glass without washing.The modified and unmodified groups were further divided b washing withCPW or without washing. which were then cultured on blood agar medium to count CFU.PM biofilms were easily detached from 10F2S difference of CFU among NC, NI and NF. But WI and WF were significantly lower than in WC. It was concluded that PM biofilms grown on the glass surface modified were easily detached compared with the unmodifiedglass groups Copyright©2017, Ayako Teranaka et al. This is an open access article distributed under the Creative Commons Att use, distribution, and reproduction in any medium, provided the original work is properly cited.
