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Antibacterial activity of P. granatum peel extracts water and ethanol extract alone or mixed with silver nanoparticles against Escherichia coli. (A) control, (B) 0.5mg/10ml peel water extract, (C) 0.5mg/10ml peel ethanol extract, (D) 0.5mg/10ml silver nanoparticles, (E) 1:1 (v/v) of 0.5mg/10ml silver nanoparticles and water peel extract, (F) 1:1 (v/v) of 0.5mg/10ml silver nanoparticles and ethanol peel extract, (G)1.0mg/10ml peel water extract, (H) 1.0mg/10ml peel ethanol extract, (I) 1.0mg/10ml silver nanoparticles, (J) 1:1 (v/v) of 1.0mg/10ml silver nanoparticles and water peel extract, (K) 1:1 (v/v) of 1.0mg/10ml silver nanoparticles and ethanol peel extract.
Source publication
Water and ethanol extracts of Punica granatum peels were prepared at the concentration 0.5 and 1.0mg/10ml, silver nanoparticles (4000mg/l) prepared at the same concentrations (0.5 and 1.0mg/10ml). Bacterial samples used in the experiment consisted of two gram-negative bacteria (Escherichia coli and Pseudomonas erogenous) and two gram-positive bacte...
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Introduction: Pomegranate (Punica granatum L.) is an ancient fruit with numerous phytochemical bioactive compounds. In this study, the antibacterial activity of ethanolic extracts of pomegranate peels and seeds were investigated against Pseudomonas aeruginosa and Staphylococcus aureus clinical isolates from Tabriz health centers (2017).
Materials a...
Citations
... To underscore the environmentally friendly attributes of the AgNPs, their UV absorption spectrum within the 200-600 nm range was meticulously recorded and compared with the UV spectrum of PgP and PnP. Te UV-Vis absorption spectra revealed the emergence of distinctive broad surface plasmon resonance peaks at 393 nm and 397 nm for PgP AgNPs and PnP AgNPs, respectively, by established literature data [27][28][29]. Te UV-Vis absorption spectra revealed the emergence of distinctive broad surface plasmon resonance peaks at 291 nm and 297 nm for PgP and PnP extracts, respectively. ...
The present study investigates an environmentally conscious method for synthesizing silver nanoparticles (AgNPs) by employing extracts from pomegranate peel (PgP) and pineapple peel (PnP). This green synthesis approach offers a sustainable alternative to traditional chemical methods, thereby reducing the ecological footprint associated with nanoparticle production. The PgP and PnP extracts serve as both reducing and capping agents during the synthesis process, enhancing the biocompatibility of the resultant AgNPs. Various characterization techniques, including UV-Vis spectroscopy, Raman analysis, X-ray diffraction (XRD), dynamic light scattering (DLS), Fourier transform infrared (FTIR), and transmission electron microscopy (TEM), were utilized to analyze the synthesized AgNPs. UV-Vis spectroscopy confirmed the formation of AgNPs through characteristic surface plasmon resonance peaks, while FTIR examined the interaction between biomaterial components and the oxidation and coating of silver nanoparticles. Raman analysis elucidated the functional groups responsible for reducing and stabilizing AgNPs, while XRD provided insights into their crystalline structure. TEM images revealed the size and morphology of the nanoparticles, while DLS characterized their average size and morphology. In addition, the synthesized AgNPs were utilized in a bioelectrochemical cell to leverage their unique properties for enhanced electrochemical performance, showcasing their potential application in energy storage and conversion systems. Overall, this study demonstrates the feasibility of utilizing agricultural waste products such as PgP and PnP for sustainable AgNP synthesis, offering promising prospects for environmentally friendly nanotechnology advancement.
... In green synthesis of AgNPs, the plant extract has a dual action of being both the reducing agent and the capping agent. Pomegranate peel extracts (PPE) elsewhere have been used for the synthesis of AgNPs and were shown to exhibit good antimicrobial activity [22][23][24]; however, literature review indicated that AgNPs prepared using Omani PPE have not yet been explored for their antioxidant and cytotoxicity activities. ...
... The formation of eco-friendly AgNPs was confirmed by recording their UV absorption spectrum in the 200-600 nm range and comparing them with the UV spectrum of PPE, quercetin, and gallic acid. Appearance of new broad surface plasmon resonance peaks at 425 nm, 421 nm, and 413 nm in the UV-Vis absorption spectra (Fig. 2) for Q-AgNP, P-AgNP, and GA-AgNP, respectively are consistent with the literature data [11,24]. Four intense peaks in the XRD spectra (2θ = 37.84-38.62°, ...
Background
Green synthesis of silver nanoparticles (AgNPs) has gained popularity due to the economical and eco-friendly approach associated with it. The aim of the study was to biosynthesize silver nanoparticles using pomegranate peel extract (PPE), quercetin (Q), and gallic acid (GA) and to evaluate their antioxidant, antimicrobial, and cytotoxic activities.
Results
A sharp color change from yellow to brown, appearance of a peak in a UV spectrum around 413–425 nm, and four intense peaks in XRD spectra matching with the reference silver crystal confirmed the formation of AgNPs. SEM and TEM analysis revealed particles to be mostly spherical with few aggregates. Average particle size distribution and zeta potential was found to be 43.6 nm and −18.01mV, 31.6 nm and −21.5 mV, and 21.7 nm and −27.9 mV for Q-AgNPs, P-AgNPs, and GA-AgNPs, respectively. P-AgNPs showed excellent in vitro antioxidant activity (84.85–89.20%) and cytotoxic activity (100% mortality). P-AgNPs and GA-AgNPs exhibited good antimicrobial activity against the four bacterial strains comparable to the reference antibiotics but Q-AgNPs failed to inhibit the growth of P. vulgaris.
Conclusions
Omani pomegranate peel extract seems to be a potential and alternative source for the simple, one pot, eco-friendly green synthesis of extracellular stable AgNPs. The synthesized nanoparticles have the potential to be developed as possible antimicrobial agents and antioxidants.
Graphical abstract
Diabetes causes lung function disorders. However, pulmonary collagen turnover under diabetes has not been well studied. The purpose of this study was to investigate collagen turnover in lung and to evaluate protective effect of pomegranate peel extract (PPE). Diabetes model in rat was induced by alloxan (250 mg/kg, i.p.). PPE treated rats received 100 mg/kg of PPE for 30 days. PPE was characterized by Gas chromatography–mass spectrometry (GC-MS). Hydroxyproline was determined by colourimetric assay kit, total collagen using ELISA kit and by histopathological assay. Collagenases (MMP-1,-8,-13) wereanalyzed by fluorometric method. Antioxidant enzyme activities and inflammatory marker C-reactive protein (CRP) were evaluated. Diabetes decreases SOD activity and increases CRP level by 33% (p < 0.05) and 125% (p < 0.01), respectively, when compared to the control group. PPE abrogated diabetesinduced SOD activity and CRP level disturbance. Collagenase activity was lower in diabetes and PPE group by 38% (p < 0.05) and 34% (p < 0.05), respectively, when compared to control. Diabetes increased significantly collagen and hydroxyproline by 32% (p < 0.05) and 192% (p < 0.01), respectively, and restored at similar level than controls in PPE group. Lung histopathological assay showed distended alveoli and peribronchial inflammatory cell infiltration in diabetic group. Compared to diabetic, PPE limits inflammatory cell infiltration in lung tissue. Diabetes causes ECM imbalance and enhances inflammation in lung wall, which can be abrogated by PPE.
The present investigation was started with aim to formulate and assess the antimicrobial potential of Punica granatum seed oil cream. The P. granatum seed oil cream was formulated using glyceryl monostearate and other lipids like stearic acid as well as cetyl alcohol. The formulated cream was then evaluated with respect to physicochemical properties and in vitro antimicrobial activity against Escherichia coli. The formulated oil loaded cream showed acceptable physicochemical properties like pH, spreadability, type of emulsion and viscosity which confer its suitability for topical application. In addition to this, the in vitro antimicrobial potential of oil loaded cream against E. coli was also found to be acceptable. Thus, formulated cream could be promising alternative for topical application of P. granatum seed oil with better antimicrobial potential.
