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An example of an incomplete sequence of the COI barcoding region of Artemia aff. sinica Cai, 1989 for amplification of the COI marker, with only <700 bp length successful, on electrophorese gel using previously described primers (Folmer et al., 1994; Munoz et al., 2008; Xu, 2018).

An example of an incomplete sequence of the COI barcoding region of Artemia aff. sinica Cai, 1989 for amplification of the COI marker, with only <700 bp length successful, on electrophorese gel using previously described primers (Folmer et al., 1994; Munoz et al., 2008; Xu, 2018).

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Due to the lack of a taxonomic key for the identification of Artemia species, molecular markers have been increasingly used for phylogenetic studies. The mt COI marker is a regularly considered marker for the molecular systematics of Artemia populations. The proposed universal and specific primers have mostly failed to amplify the Artemia aff. sini...