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Amino acid composition of marine collagen peptides 

Amino acid composition of marine collagen peptides 

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Marine collagen peptides (MCP) have been reported to exhibit antioxidative activity, which is the common property of numerous hepatoprotective agents. Previous studies have shown that MCP have biological functions including anti-hypertension, anti-ulcer, anti-skin ageing and extending the life span. However, its role in alcoholic liver injury remai...

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... the main composition of MCP was oligopeptides. The amino acid composition of MCP is shown in Table 1. ...
Context 2
... the present study, female rats were adminis- tered with alcohol at a dose of 6 g/kg for 4 weeks to cause early liver injury. The results showed a reduced body weight † † 35 30 25 20 15 10 5 0 80 70 ...

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... As protein degradation products, peptides play an essential role in the development of various liver disorders. In the investigation of Lin et al., marine collagen peptides inhibited early alcoholic liver injury in female rats by improving oxidative stress and lipid metabolism (33). Lv et al. observed that maize peptides significantly reduced MDA, NO, hydroxyproline (HYP), transforming growth factor-β1 (TGF-β1) levels and lactate dehydrogenase activity in the liver and substantially increased the SOD level. ...
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... Various studies demonstrated that the ingestion of bioactive peptides, derived from corn , Chum Salmon skin collagen (Lin et al., 2012), marine fungus (Cai, Yan, Fu, & Wang, 2017), and chicken liver etc. (Lin et al., 2017), could protect rodent models against alcohol-induced liver injury through oxidative reduction. Chicken proteins are considered ideal sources of bioactive peptides due to nutritional and health properties (Cui, Zhou, Zhao, & Yang, 2009). ...
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... 9 Compared with the above, bioactive peptides are more easily absorbed, more stable and safer. 10 Exogenous bioactive peptides, such as mushroom polysaccharide-peptides, 11 coriolus versicolor polysaccharide peptides, 12 Ganoderma peptides, 13 seaweed Laminaria japonica peptides, 14 and corn peptides, 15 have been testified to relieve liver damage. Regardless of great efforts, the prevention against ALD remains a challenge. ...
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... Collagen is a major component related to extracellular matrix proteins in multicellular animals including humans, and plays an important role in maintaining a cytoskeleton. Furthermore, it has been widely used as a biomaterial (e.g., as a scaffold for cell and tissue regeneration-related proteins, wound dressing, or dietary supplement) [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17] because of its biocompatibility. In particular, collagen peptides, which are enzymatically hydrolyzed from gelatin, are often administered orally because they are easily digestible 18) , and have been reported to have a positive effect on skin, cartilage and bone [19][20][21][22][23][24][25][26][27][28][29][30][31][32] . ...
... Zang et al. reported that oral administration of collagen peptides derived from Chum salmon skin promoted cutaneous wound healing and angiogenesis through upregulation of vascular endothelial growth factor (VEGF) and fibroblast growth factor (FGF)-2 expression in rats, demonstrating the potential role of FCP as a healing material 38) . Furthermore, the administration of collagen peptides from Chum salmon has also been reported to have a protective effect against early alcoholic liver injury by interfering with increased levels of total cholesterol and triacylglycerol 16) and to have an accelerated healing effect on gastric ulcers 15) . Thus, it has been shown to be effective for wound healing of mucous membranes as well as skin. ...
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Fish collagen peptides (FCP) derived from the skin, bones and scales are commercially used as a functional food or dietary supplement for hypertension and diabetes. However, there is limited evidence on the effects of FCP on the osteoblast function in contrast to evidence of the effects on wound healing, diabetes and bone regeneration, which have been obtained from animal studies. In this narrative review, we expound on the availability of FCP by basic research using osteoblasts. Low-concentration FCP upregulates the expression of osteoblast proliferation, differentiation and collagen modifying enzyme-related genes. Furthermore, it could accelerate matrix mineralization. FCP may have potential utility as a biomaterial to improve collagen quality and promote mineralization through the mitogen-activated protein kinase and Smad cascades. However, there are few clinical studies on bone regeneration in human subjects. It is desirable to be applied clinically through clinical study as soon as possible, based on the results from basic research.
... However, the evidence that evaluates the biological efficacy of these peptides in animal or clinical studies is relatively limited. Studies have shown that oral administration of ACE inhibitory peptides derived from bovine bone gelatin, squid skin gelatin, jellyfish collagen, salmon skin gelatin, and porcine skin collagen can significantly reduce the blood pressure of spontaneously hypertensive rats (SHRs) (Cao, Wang et al. 2020;Ichimura et al. 2009;Jung, Joong-Kyun, and Hee-Guk 2014;Lin, Zhang, et al. 2012;Zhuang, Sun, and Li 2012). Zhuang, Sun, Zhang et al. (2012) proved that feeding jellyfish collagen peptide (100 mg/kg, 30 days) to renovascular hypertensive rats could significantly reduce their blood pressure by inhibiting the concentration of Ang II in the kidney, indicating that the kidney could act as a potential target here. ...
... Wang's investigation showed that with the administration of tilapia skin gelatin hydrolysate for 30 days, glucose tolerance in rats with streptozotocin-induced diabetes improved, while the glucagon-like peptide 1 and insulin secretion were enhanced . MCP shows a protective effect on early alcoholic liver injury in rats via anti-oxidative activity and improved lipid metabolism (Lin, Zhang, et al. 2012). ...
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... Bioactive peptides maintained proper liver functioning, up-regulated the nitric oxide levels by reducing the levels of pro-inflammatory cytokines (Koyama and Kusubata, 2013). Marine collagen peptides were found to be proficient in preventing the alcoholic liver injury (Lin et al. 2012). Great hammerhead shark (S. mokarran) is one of the highly demanded species for their fins, meat and liver oil world wide. ...
... Hepatic atrophy can be characterized by decreased cytoplasm to nuclei ratio. Previous studies demonstrated the capability of marine collagen peptides to reverse the alcohol induced liver damage in rats (Lin et al. 2012). Figure 5A, B illustrate the activity levels of the antioxidant defense enzymes SOD and catalase in serum. ...
... SOD enzyme neutralizes the superoxide free radicals into hydrogen peroxide, consecutively catalase enzyme mediates the (Guo et al. 2009). Marine collagen peptides were previously reported to exhibit antioxidant activity to counteract alcohol induced oxidative stress in Wistar rats (Lin et al. 2012). These class of bioactive peptide include metal ion binding proteins which chelates metal ions and, simultaneously prevent the formation of free radicals. ...
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The aim of the present study was to evaluate the ability of fish collagen peptides (FCP) derived from the skin of great hammerhead shark (Sphyrna mokarran) in attenuating the high fat diet-alcohol induced hyperlipidemia. The oral supplementation of FCP in high fat diet-alcohol fed experimental rats confirmed the regulation of body weight to normal level. The FCP treated group revealed the efficient lipid lowering ability by enhancing the cholesterol metabolism. Western blot analysis of the lipid metabolic enzymes revealed that the oral-intake of FCP has down-regulated the expression levels of fatty acid synthase and 3-Hydroxy-3-methylglutaryl-CoA reductase (HMGCR). Simultaneously, the expression levels of Lecithin-cholesterol acyltransferase (LCAT) in liver was up-regulated. Histopathology analysis of liver tissues demonstrated that the FCP treated group maintained normal liver parenchyma with moderate inflammatory infiltration, whereas the statin treated group developed centrilobular fibrosis, atrophy of hepatocytes and moderate inflammatory infiltration. Oral dietary supplementation of FCP enhanced the activity levels of both superoxide dismutase and catalase enzymes and, lowered the levels of lipid peroxidation in liver tissues. Supplementary information: The online version contains supplementary material available at 10.1007/s13197-021-05118-0.
... Collagen hydrolysate (also referred to as gelatin hydrolysate or collagen peptide) is prepared by enzymatic hydrolysis of gelatin and is widely used as a food supplement for its effects on health and beauty. Oral ingestion of collagen hydrolysate is reported to have various beneficial effects, such as enhancement of bone strength (Konig et al., 2018;Wu et al., 2004), attenuation of joint pain (Benito-Ruiz et al., 2009;Clark et al., 2008), improvement of skin conditions (Oba et al., 2013;Proksch et al., 2014) and reduction of blood lipids (Lin et al., 2012;Saito et al., 2009;Tak et al., 2019;Woo et al., 2018). The above reports include clinical studies evaluating bone (Konig et al., 2018), joint (Benito-Ruiz et al., 2009;Clark et al., 2008), skin (Proksch et al., 2014) and lipid metabolism (Tak et al., 2019). ...
... For example, single administration of collagen hydrolysate suppressed the triglyceride (TG) absorption (Saito et al., 2009). Continuous administration of collagen hydrolysate decreased blood and tissue lipid levels in alcohol-induced liver injury model rats (Lin et al., 2012) and mice fed a high-fat diet (Woo et al., 2018). However, there has been little focus on the effect of continuous ingestion of collagen hydrolysate on lipid metabolism in normal animals with mild modification of the diet composition. ...
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Collagen hydrolysate has various beneficial effects, such as bone strengthening, joint/skin protection and lipid metabolism regulation. In this study, the anti-obesity activity of ginger protease-degraded collagen hydrolysate (GDCH) was evaluated in BALB/c mice fed diets containing 14% casein (control group) or 10% casein + 4% GDCH (GDCH group) for 10 weeks. In the GDCH group, triglyceride (TG) and cholesterol (CHO) levels in blood and adipocyte size in white adipose tissue were significantly decreased compared with those of the control group. Further, gene expression related to fatty acid synthesis, such as acetyl-CoA carboxylase, fatty acid synthase and stearoyl-CoA desaturase, was decreased in the liver and white adipose tissue of GDCH-fed mice. On the other hand, single oral administration of GDCH did not result in decrease in blood TG and CHO compared with vehicle and casein in ICR mice pre-administered soybean oil. These results suggest that the GDCH-induced decreases in tissue and blood lipids occur through long-term alterations in lipid metabolism, not transient inhibition of lipid absorption. The lipid-lowering effects exhibited by partial substitution of casein with GDCH imply the possibility that daily supplementation of GDCH contributes to prevention/attenuation of obesity and hyperlipidemia.
... The tyrosine content of 90G10C was lower than that of the other gels; therefore, a longer exposure time was needed to maintain the free radicals to achieve a reaction. The content of tyrosine in collagen is only 0.03 in 100 residues [59], and the collagen used in the study consisted of fibrils rather than triple helix molecules. In addition, a dityrosine bond can only form between two nearby tyrosine molecules. ...
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Most gelatin hydrogels used in regenerative medicine applications today are fabricated by photocrosslinking due to the convenience and speed of this method. However, in most cases photoinitiators are used, which require UV light, which, in turn, can cause cell and tissue damage, or using functionalized gelatin. Recently, ruthenium (II) tris-bipyridyl chloride has been studied as an initiator that can induce dityrosine bond formation using visible light. In addition, continuous fibrils and small particles are often used to reinforce composite materials. Therefore, this study investigated the visible-light-induced photocrosslinking of native gelatin molecules via dityrosine bonds formation as well as gel reinforcement by collagen fibrils and mesoporous bioactive glass (MBG) particles. The results show that collagen and MBG exerted a synergistic effect on maintaining gel integrity with a dental LED curing light when the irradiation time was shortened to 30 s. Without the two reinforcing components, the gel could not form a geometric shape stable gel even when the exposure time was 120 s. The shear strength increased by 62% with the collagen and MBG compared with the blank control. Furthermore, our results demonstrate that the addition of collagen and MBG enhanced gel stability in an artificial saliva solution. These results demonstrate the considerable advantages of using tyrosine-containing biomolecules, and using a dental LED curing light for the crosslinking of hydrogels in terms of their suitability and feasibility for use as bioadhesives in confined clinical working space, such as the oral cavity, and in application as in situ-crosslinked injectable hydrogels.
... Wang et al. gave rats 50% alcohol at a dose of 12 ml/kg for 8 days [40], and another different method was that the mice orally received 2.4 g/kg of ethanol for 15 days [41]. Other researchers gave rats 6 g/kg of alcohol for 4 weeks [42]. In the preliminary assay, the serum levels of ALT, AST, and TG were all significantly increased after 4 weeks of alcohol treatment in rats. ...
... In the present study, the ratio of AST/ALT was increased in the alcohol group compared with normal control, which indicated an advanced ALD, although no significant change was observed (Table 2). A similar result was also reported by Lin et al. [42]. YGMM treatment inhibited the increase in AST/ALT ratio (Table 2), which may alleviate alcoholic hepatic injury. ...
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Background: Yigan mingmu oral liquid (YGMM) is a herbal medicine based on a famous Chinese herbal formula that has been used for sore eyes for more than 400 years. Eye health is closely associated with the liver based on TCM. This study aimed to investigate the hepatoprotective effect of YGMM against acute liver injury induced by alcohol in rats. Methods: Experimental rats were administered with silymarin and YGMM through the gastric gavage during the entire experiment. Starting from the 11th day, the rats were administered orally with 14 ml/kg Red Star Erguotou Liquor, a popular brand, at 4 h after the dose of silymarin (100 mg/kg) and YGMM (1, 2.5 and 5 ml/kg in low, middle and high dosage group, respectively) once a day for 4 weeks except for the rats in the normal group. Biochemical parameters, including ALT, AST, TB, TG, T-SOD, GSH, and MDA were detected to evaluate the protective effect of YGMM. Pathological changes were observed through histopathological examination. Results: Treatment with YGMM exhibited a significant protective effect by reversing the biochemical parameters (ALT, AST, TB, TG, and GSH) and histopathological changes. Histopathological examination by Oil Red O Staining Solution showed that lipid droplets were significantly reduced in the silymarin and YGMM groups (p < 0.001) when compared to alcohol group. Conclusions: YGMM exhibits a significant hepatoprotective activity against acute liver injury induced by alcohol in rats.
... Such bioactive peptides can exert various bioactivities after being released from parent protein, including antioxidant, antihypertensive, antidiabetic, anti-inflammatory, antimicrobial, mineral binding, hepatoprotective effect, etc., in addition to nutritional value (Mora, Gallego, & Toldrá, 2018). To date, peptides derived from various proteins, including corn (Yamaguchi, Nishikiori, Ito, & Furukawa, 1997;Yu, Li, He, Huang, & Zhang, 2013;, Chum Salmon skin collagen (Liang et al., 2014;Lin et al., 2012), mushroom (Zhao et al., 2017), and chicken meat protein (Lin et al., 2017), have been reported to facilitate alcohol metabolism. However, few researches have been focused on the isolation and identification of novel peptides exerting ADH stabilizing activity. ...
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The effect of chicken hydrolysates (CHs) on alcohol dehydrogenase (ADH) stability was investigated, together with further bioactivity-oriented isolation and identification of CHs. A total of 82 peptides were identified using mass spectrometry in tandem after consecutive separation by size-exclusion chromatography and high-performance liquid chromatography. The identified peptides were then subjected to in silico gastrointestinal digestion and 154 peptides were generated. The potential bioactivity, safety and applicability of the peptides were assessed using multiple predictive programs. A total of 21 among the 154 peptides were predicted to be potentially active with applicability. Four peptides (DPQYPPGPPAF, QKPVL, KPC, and APGH) obtained after in silico digestion were synthesized and validated their activity. Results showed that DPQYPPGPPAF, KPC, and APGH could stabilize ADH in a dose-dependent manner. This study further indicated that chicken hydrolysate could be a novel functional food ingredient in facilitating alcohol metabolism and protection against alcoholic liver injury.