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... to alignment, the raw fluorogram sequences were visually inspected and cleaned up using Chromas LITE Version 2.1.1. Sequences were aligned against 7 16S rDNA and 16 D- Loop region corresponding sequences of P. antarctica retrieved from the nucleotide database NCBI (Fig. 3, see Table 1 for GenBank accession numbers). Alignment was then performed by MUSCLE (http://www.ebi.ac.uk/Tools/msa/muscle/) using MEGA 6.06 ( Tamura et al. 2013). Three closely related notothenioids Aethotaxis mitopteryx, Dissostichus mawsoni and Dissostichus eleginoides (Near et al. 2012) were included as outgroups (Fig. 3, see Table ...
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... database NCBI (Fig. 3, see Table 1 for GenBank accession numbers). Alignment was then performed by MUSCLE (http://www.ebi.ac.uk/Tools/msa/muscle/) using MEGA 6.06 ( Tamura et al. 2013). Three closely related notothenioids Aethotaxis mitopteryx, Dissostichus mawsoni and Dissostichus eleginoides (Near et al. 2012) were included as outgroups (Fig. 3, see Table 1 for GenBank accession numbers). The proportion of nucleotide positions at which two sequences differed represented by the p-distance was used to calculate genetic distance in MEGA. Based on these distances, a neighbour-joining tree was drawn using the Kimura 2-parameter substitution model, with tree robustness confirmed by ...
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