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AMPK activation is decreased with aging in human skin. Discarded skin was obtained from patients undergoing a surgical procedure. (a) The samples were processed for Western blot to evaluate AMPK activation levels using p-T172 AMPK and total AMPK (arrow) antibodies. GAPDH was used as a loading control. (b) The ratios of p-T172 to total AMPK were significantly lower in the samples from individuals over 50 years old (n = 6) compared to samples from individuals less than 20 years old (n = 6). doi:10.1371/journal.pone.0115341.g008 

AMPK activation is decreased with aging in human skin. Discarded skin was obtained from patients undergoing a surgical procedure. (a) The samples were processed for Western blot to evaluate AMPK activation levels using p-T172 AMPK and total AMPK (arrow) antibodies. GAPDH was used as a loading control. (b) The ratios of p-T172 to total AMPK were significantly lower in the samples from individuals over 50 years old (n = 6) compared to samples from individuals less than 20 years old (n = 6). doi:10.1371/journal.pone.0115341.g008 

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The aging process is perceived as resulting from a combination of intrinsic factors such as changes in intracellular signaling and extrinsic factors, most notably environmental stressors. In skin, the relationship between intrinsic changes and keratinocyte function is not clearly understood. Previously, we found that increasing the activity of AMP-...

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... AMPK activation is decreased in skin from aged individuals. Our data suggest that decreased AMPK activity may be a mediator of cellular age-related changes in skin. To assess whether it might play such a role in humans, we examined human skin that had been discarded during surgical procedures. As shown in Fig. 8, samples from patients over 50 yrs. old had lower AMPK activity (as illustrated by a lower pAMPK/tAMPK ratio) than skin from younger patients (under 20 yrs. old) Although these data provide support for decreased AMPK activity as an accompaniment of skin aging, the small sample size (n = 6) and incomplete patient char- acterization necessitate confirmation of such observations in future studies. Likewise, ...

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