| A secondary broad analysis demonstrated a correlation between paramyxovirus bidirectional sequence reads with LVEF pre and post chemotherapy, but with greater correlation and significance post chemotherapy LVEF (A). Similar correlations were detected for orthomyxovirus both pre and post chemotherapy (B). Retrovirus reads had no detectable correlation with LVEF pre chemotherapy, but a trend to increased correlation post chemotherapy (C). Analysis of all RNA virus reads for paramyxovirus, orthomyxovirus and retrovirus reads detected a significant correlation for changes in LVEF post chemotherapy (D).

| A secondary broad analysis demonstrated a correlation between paramyxovirus bidirectional sequence reads with LVEF pre and post chemotherapy, but with greater correlation and significance post chemotherapy LVEF (A). Similar correlations were detected for orthomyxovirus both pre and post chemotherapy (B). Retrovirus reads had no detectable correlation with LVEF pre chemotherapy, but a trend to increased correlation post chemotherapy (C). Analysis of all RNA virus reads for paramyxovirus, orthomyxovirus and retrovirus reads detected a significant correlation for changes in LVEF post chemotherapy (D).

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Background Viral infections are pervasive and leading causes of myocarditis. Immune-suppression after chemotherapy increases opportunistic infections, but the incidence of virus-induced myocarditis is unknown. Objective An unbiased, blinded screening for RNA viruses was performed after chemotherapy with correlation to cardiac function. Methods Hi...

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... sequence detection and retrovirus sequence detection, using bidirectional reads from this more permissive analysis, had increased correlation when comparing LVEF post chemotherapy and LVEF measured pre chemotherapy; For paramyxovirus the correlation increased from r = 0.484 pre chemotherapy LVEF (P < 0.0452) to r = 0.592 for LVEF measured post chemotherapy (P < 0.0096, Figure 4A), with a clear increase in significance. Albeit both achieve significance. ...
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... other sequences detected there were Molivirus, Pandoravirus and Pandoravirus dulcis, Lambdina fiscellaria A potential correlation between changes in LVEF pre and post chemotherapy with an analysis of a combined RNA virus sequence reads for virus sequence detection where trends toward increased RNA viral sequence correlations with reduced LVEF were demonstrated was then examined. A combined read for detcted paramyxo-orthomyxo-and retro-virus sequnces was assessed using this broad screen ( Figure 4D). On this final analysis of combined RNA virus sequences for each sample, the correlation increased from r = 0.233 (p = 0.3732) for pre chemo LVEF to r = 0.621 (P < 0.0078) for post chemo LVEF, a markedly significant increase. ...
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... this final analysis of combined RNA virus sequences for each sample, the correlation increased from r = 0.233 (p = 0.3732) for pre chemo LVEF to r = 0.621 (P < 0.0078) for post chemo LVEF, a markedly significant increase. Thus, increased detection of three detectable RNA virus sequence reads, paramyxo, orthomyxo-and retro-viruses after chemotherapy did demonstrate an apparent significant association with measured reductions in LVEF after chemotherapy ( Figure 4D). ...
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... increase in the paramyxovirus Avulavirus, sequences does demonstrate an increase in sequence detection and reduced LVEF after chemotherapy, with a greater correlation for post chemotherapy LVEF than for pre-chemotherapy LVEF. Orthomyxovirus influenza A was also detected, but with similar detected sequence counts and correlations for LVEF measured pre-or post-chemotherapy ( Figure 4B); this correlation was stronger for pre-chemotherapy. Influenza is a common upper respiratory infection and the detection of this orthomyxovirus may be attributed to the prevalence of influenza A virus in the general population, with a generalized increase in an immunocompromised cohort. ...
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... suggests a general association of influenza A, the orthomyxovirus, with a reduction in LVEF that is unrelated to chemotherapy. The paramyxovirus Avulavirus sequence detection was also detectable, but the correlation was greater for LVEF measured after chemotherapy, albeit a small increase ( Figure 4A). On the secondary, broader analysis, the greatest increase in correlation was seen with the HERV K retrovirus sequence on the post chemotherapy sample. ...
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... the secondary, broader analysis, the greatest increase in correlation was seen with the HERV K retrovirus sequence on the post chemotherapy sample. Simple regression analysis for pre-chemotherapy LVEF analysis exhibited a flat, unresponsive regression with no evidence for correlation with LVEF pre chemotherapy, but with a clear inverted, negative correlation for detected HERV K sequences with post-chemotherapy LVEF ( Figure 4C). ...