Figure - available from: Archives of Virology
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(A) Sequence alignment the RdRp motifs of LAV2 and selected members of the genus Amalgavirus. Horizontal black lines above the sequence alignment indicate the conserved motifs I to VII. Shaded areas indicate identical aa residues. LAV2 is indicated by a red star. (B) Phylogenetic analysis of LAV2 and other selected viruses based on aa sequences of ORF2 (RdRp). Sequences of ORF2 translation products were aligned using CLUSTAL X and then subjected to phylogenetic analysis using IQ-TREE. The tree is displayed as a rectangular phylogram rooted on the branch to the family Amalgaviridae, Partitiviridae, and Totiviridae members. Branch support values are shown as percentages. The scale bar indicates a genetic distance of 0.5 aa substitutions per site. LAV2 is indicated by a red star.
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A novel plant virus with a double-stranded (ds) RNA genome was detected in Lilium spp. in China by high-throughput sequencing and tentatively named “lily amalgavirus 2” (LAV2). The genomic RNA of LAV2 is 3432 nucleotides (nt) in length and contains two open reading frames (ORFs) that putatively encode a ‘1 + 2’ fusion protein of 1053 amino acids (a...
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Citations
... The partial ORF1 and ORF2 encode an RdRp fusion protein resulting from a frameshift at the + 1 PRF motif, consisting of 1,057 amino acids with a predicted molecular mass of 118.6 kDa (Fig. 1C). An alignment of the amino acid sequence of the RdRp encoded by SGAV1 with those of 10 related amalgaviruses revealed that the SGAV1 RdRp contains all seven conserved motifs (I to VII) ( Supplementary Fig. S1A) described previously [8,9]. ...
A novel monopartite dsRNA virus, tentatively named “sponge gourd amalgavirus 1” (SGAV1), was discovered by high-throughput sequencing in sponge gourd (Luffa cylindrica) displaying mosaic symptoms in Jiashan County, Zhejiang Province, China. The genome of SGAV1 is 3,447 nucleotides in length and contains partially overlapping open reading frames (ORFs) encoding a putative replication factory matrix-like protein and a fusion protein, respectively. The fusion protein of SGAV1 shares 57.07% identity with the homologous protein of salvia miltiorrhiza amalgavirus 1 (accession no. DAZ91057.1). Phylogenetic analysis based on the RNA-dependent RNA polymerase (RdRp) protein suggests that SGAV1 belongs to the genus Amalgavirus of the family Amalgaviridae. Moreover, analysis of SGAV1-derived small interfering RNAs indicated that SGAV1 was actively replicating in the host plant. Semi-quantitative RT-PCR showed higher levels of SGAV1 expression in leaves than in flowers and fruits. This is the first report of a novel amalgavirus found in sponge gourd in China.
