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A. Microfluidic membrane device schematic. B. Cellulose fibrils are maintained in thin film of Cel7A-medium fluid that allows probing with IR light. Soluble enzyme is supplied from the media channel below the membrane. C. Device. D, E. Results of Computational Fluid Dynamic (CFD) simulation of the temperature and relative humidity fields for the device which are consistent with values from the temperature and humidity sensors.
Synchrotron infrared hyperspectral microscopy is a label-free and non-invasive technique well suited for imaging of chemical events in situ. It can track the spatial and temporal distributions of molecules of interests in a specimen in its native state by the molecule's characteristic vibrational modes. Despite tremendous progress made in recent ye...
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... polydiemthylsilonxane (PDMS) device (Figure 1) composed of a gold-coated silicon nitride porous membrane between a feeding channel and a humidity controlled viewing chamber. It allows cellulose to be attached and maintained on the upper membrane surface in a micrometer-thick layer of enzyme solution while buffer media is replenished from the feeding microchannels below. ...
... hydrolysis study was performed (Figure 2) and the optimum flow rate ratio for the two fluid inputs (Figure 1) was 1:2.5 (DI water in the upper channels: Cel7A-buffer medium in the lower channels) to maintain a micrometer thick liquid film and pH during live infrared imaging of hydrolysis. Algal cellulose fibrils purified from C. aegagropila were deposited onto the porous membrane and incubated overnight in the refrigerator for attachment. ...