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A Dose-dependent reduction in slime synthesis of MRSA upon treatment with increasing concentrations of Cannabis extract demonstrated by the Congo red agar (CRA) assay. B Inhibition of EPS production in MRSA in the presence of Cannabis extract as examined by the phenol–sulphuric acid method of polysaccharide quantification. The results were calculated as means ± SD (n = 3)
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Antimicrobial resistance (AMR) poses a serious threat to human, animal, and plant health on a global scale. Search and elimination techniques should be used to effectively counter the spread of methicillin-resistant Staphylococcus aureus (MRSA) infections. With only a few novel drugs in clinical development, the quest for plant-based alternatives t...
Citations
... The culturally significant and versatile Carica papaya plant has several applications, including in medicine, the kitchen, and industry. Because of its high nutrient content and potential health benefits, it is widely cultivated in tropical regions across the world (Roshan et al., 2024). Traditional medicine has long been acknowledged for its ability to treat a wide range of diseases. ...
The purpose of this study is to compare the antimicrobial properties of natural extracts from both Carica papayaleaf and Syzygium cumini seeds. The extracts were tested against typical bacterial species using traditionalagar diffusion methods including Bacillus subtilis, Staphylococcus aureus, Escherichia coli, and Salmonellatyphi. For both extracts, the findings showed notable antibacterial action; Syzygium cumini showed moreinhibitory zones than Carica papaya. Both extracts exhibited bioactive components including tannins andflavonoids in phytochemical analysis, which most certainly help to explain their antibacterial action. Using thiscomparative analysis, the therapeutic uses of natural plant extracts as alternative antibacterial agents arehighlighted, therefore offering an understanding of their possibilities in the prevention of bacterial infections.More investigation is needed to clarify the processes behind their antibacterial properties and their possibilitiesin therapeutic environments.
... For antimicrobial activity testing, Mueller-Hinton agar (MHA) was added to Petri dishes. A 0.5 mL suspension of MRSA bacteria, prepared to match the McFarland standard of 0.5, was evenly spread across the surface of the MHA using a sterile swab [19]. The Petri dishes were then divided into three sections, one each for negative control, positive control, and test samples. ...
This study explored the antimicrobial potential of Piper betle L. (PBL) and Piper nigrum L. (PNL) extracts against MRSA. Plant parts including stem, leaf, and fruit were extracted using aquadest, methanol, and hexane, resulting in 18 distinct extracts. FT-IR combined with cluster analysis (CA) categorized the extracts, and anti-MRSA activity was assessed through the paper disk diffusion method. The most potent extracts were further analyzed using GC-MS to identify bioactive compounds. Additionally, molecular docking studies were conducted for MRSA protein targets (4DKI, 6H5O, and 4CJN). The hexane extract of PNL and the aqueous extract of PBL fruit showed the strongest inhibitory effects. GC-MS identified piperine (14.22%) and diisooctyl phthalate (14.67%) as major compounds, with piperolein B, piperanine, β-caryophyllene oxide, and α-caryophylladienol as minor compounds in the hexane extract of PNL, while hydroxychavicol (81.89%) and chavibetol (12.01%) were predominant in the aquadest extract of PBL. Molecular docking revealed that piperolein B and piperine had strong binding affinities to MRSA proteins 4DKI, 6H5O, and 4CJN, comparable to ciprofloxacin. In conclusion, this study confirms the potential of PBL and PNL as sources of novel anti-MRSA agents, supporting further research to develop new therapies.
... For antimicrobial activity testing, Mueller-Hinton agar (MHA) was added to Petri dishes. A 0.5 mL suspension of MRSA bacteria, prepared to match the McFarland standard of 0.5, was evenly spread across the surface of the MHA using a sterile swab [19]. The Petri dishes were then divided into three sections, one each for negative control, positive control, and test samples. ...
This study explored the antimicrobial potential of Piper betle L. (PBL) and Piper nigrum L. (PNL) extracts against MRSA. Plant parts including stem, leaf, and fruit were extracted using aquadest, methanol, and hexane, resulting in 18 distinct extracts. FT-IR combined with cluster analysis (CA) categorized the extracts, and anti-MRSA activity was assessed through the paper disk dif-fusion method. The most potent extracts were further analyzed using GC-MS to identify bioac-tive compounds. Additionally, molecular docking studies were conducted for MRSA protein targets (4DKI, 6H5O, and 4CJN). The hexane extract of PNL and the aqueous extract of PBL fruit showed the strongest inhibitory effects. GC-MS identified Piperine (14.22%) and Diisooctyl phthalate (14.67%) as major compounds, with Piperolein B, Piperanine, Beta-caryophyllene ox-ide, and Alpha-caryophylladienol as minor compounds in the hexane extract of PNL, while hy-droxychavicol (81.89%) and Chavibetol (12.01%) were predominant in the aquadest extract of PBL. Molecular docking revealed that Piperolein B and Piperine had strong binding affinities to MRSA proteins 4DKI, 6H5O, and 4CJN, comparable to ciprofloxacin. In conclusion, this study confirms the potential of PBL and PNL as sources of novel anti-MRSA agents, supporting fur-ther research to develop new therapies.
