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(A) Amplification of the LAV2 genome by 5’ and 3’ RACE and RT-PCR. M, GL DNA Marker 5000. (B) The predicted secondary structures of the 5’ and 3’ UTRs of LAV2. (C) Schematic representation of the genome organization of LAV2.

(A) Amplification of the LAV2 genome by 5’ and 3’ RACE and RT-PCR. M, GL DNA Marker 5000. (B) The predicted secondary structures of the 5’ and 3’ UTRs of LAV2. (C) Schematic representation of the genome organization of LAV2.

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A novel plant virus with a double-stranded (ds) RNA genome was detected in Lilium spp. in China by high-throughput sequencing and tentatively named “lily amalgavirus 2” (LAV2). The genomic RNA of LAV2 is 3432 nucleotides (nt) in length and contains two open reading frames (ORFs) that putatively encode a ‘1 + 2’ fusion protein of 1053 amino acids (a...

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... The partial ORF1 and ORF2 encode an RdRp fusion protein resulting from a frameshift at the + 1 PRF motif, consisting of 1,057 amino acids with a predicted molecular mass of 118.6 kDa (Fig. 1C). An alignment of the amino acid sequence of the RdRp encoded by SGAV1 with those of 10 related amalgaviruses revealed that the SGAV1 RdRp contains all seven conserved motifs (I to VII) ( Supplementary Fig. S1A) described previously [8,9]. ...
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A novel monopartite dsRNA virus, tentatively named “sponge gourd amalgavirus 1” (SGAV1), was discovered by high-throughput sequencing in sponge gourd (Luffa cylindrica) displaying mosaic symptoms in Jiashan County, Zhejiang Province, China. The genome of SGAV1 is 3,447 nucleotides in length and contains partially overlapping open reading frames (ORFs) encoding a putative replication factory matrix-like protein and a fusion protein, respectively. The fusion protein of SGAV1 shares 57.07% identity with the homologous protein of salvia miltiorrhiza amalgavirus 1 (accession no. DAZ91057.1). Phylogenetic analysis based on the RNA-dependent RNA polymerase (RdRp) protein suggests that SGAV1 belongs to the genus Amalgavirus of the family Amalgaviridae. Moreover, analysis of SGAV1-derived small interfering RNAs indicated that SGAV1 was actively replicating in the host plant. Semi-quantitative RT-PCR showed higher levels of SGAV1 expression in leaves than in flowers and fruits. This is the first report of a novel amalgavirus found in sponge gourd in China.