Another option which comes to my mind is the "Divina Commedia" (The divine Comedy) from Dante Alighieri. In this work (of course, is not a novel) the author puts a lot of the mathematical/theological ideas of that time to build a "divine" world which is run by rules and that has a precise (i would say) "geometrical" structure.

Urvesh Patel....Thanks a lot for sparing out time for my question. As you say adding serum will create almost a bodily environment in vitro, well, that is not significant for the experiment as the drugs are gonna be screened at primary level only. the only concern was if we can store those dilutions for a week and reuse them at different time-points or not , because the drugs will be degrading eventually .

try freeling a c++ library

I think it is possible to see changes in response to climate change/variability, but it is difficult and ultimately undesirable to factor out other drivers of change. As argued in several of my publications, if we start with the premise that adaptation is a real, on-going socio-technical process, then factoring out climate variability from climate change, or factoring out non-climate variables from climate variables, is a useless and misguided effort. Instead, it makes more sense to take an integrative and holistic perspective on adaptation/change as a process that take place in real places and in complex ways. I have sometimes met resistence to this perspective amongst global systems modelers, because implies that models are not the central reference point for adaptation. This is not to say that they are without use, but that we need to identify their utility within the context of real ongoing adaptation processes.

you can prepare a stock solution and keep it at -20ºC

I posted the figure again and found that I couldn't open it either. I am a rookie at the researcegate. I wonder tha if I can deliver the figure to you and you do me a favor to post it at the researchgate. Thank you very much!

I agree with all of the above. If I want to guarantee the drug enters the bloodstream rapidly then I go I.V. via tail vein. This is not a procedure for those who haven't done this before though as it really does take practice and some just can't manage it (particularly in pigmented mice). As regards ip injections, when I inject cannabinoids I see effects after 10-15 minutes as a general rule. I don't see a problem with dexamethasone but wonder whether annexin will gain entry to the bloodstream (I'd go iv with this).

Yes you can and I agree with Dmitry. We use Percoll 51% (prepare a stock e.g for 100ml: Percoll [Sigma 71644] 51 ml, Water 38.5 ml, 10X HBSS [Gibco 14185] 10 ml, Sodium bicarbonate solution [Sigma S8761] 0.5 ml). Remember to centrifuge without beak after you add you L15 medium cell suspension on Percoll layer, you'll find leucocytes at the interface layer, so you can carefully collect them using a Pasteur pipette (top:L15 medium-brown layer:cell suspension-Percoll-bottom:erythrocytes). then follow with the other steps. Hope it is useful!

How about adding RNase A and Potease K

Well, one approach is to react an aryl magnesium halide with a diazonium BF4. Works!

I understand that. I'm merely a little puzzled why one would use one symbol with two different definitions. Maybe you should consider additional indices to clearify whether work is done by the system or the environment.

It might be that the fluid is at a similar density to the virus, meaning that it acts as a cushion. I assume that you are using something like HBSS or similar to make the volume back up? This will decrease the density of the allantoic fluid, and enable you to spin the virus out of suspension.

KapaBiosystem in South Africa has long range DNA polymerases series that you may wanna look at.

In phytoplankton ecology, stability is often considered a hypothetical situation where a maximum of three species dominate the assemblage for, at least, three weeks without considerable change in total biomass. Some useful information on this topic can be found in a Special Issue (502) of the journal Hydrobiologia published by Springer in 2003.

Dear Peter, that is exactly my theory - give the total economy an absolute value which is measurable by one physical unit. I very well know that no economic theory is saying this is possible. But I already convinced some economists by long discussions - these economists are also physicians knowing the real world better than economists. I just want to say: Be careful in saying "that does not exist" - better say "that does not exist until today". My theory is now five years old - and I started publishing it this january after some years of trying to argue myself out. I was not successful in finding loopholes. The value of a total economy is bound to physics - and it is totally plausible and there is no wrong conclusion in my theory. I am reading most of the old important books - and I find so many logically wrong conclusions ... because of the wrong question, the wrong perspective. I know that it sounds strange - but I already convinced some econonmists who are also physicists. And for your last sentence: It is absolutely not senseless to search for this question. At first it is - in my opinion - always good to search for better answers. And to have an economic theory binding macroeconomics to real masurable units is - in my opinion - a better answer. Second this gives new answers to our big problems of today. Why are we in an financial mess nearly all over the world? Maybe the answer is that the energy driving most of our worldwide economy does not grow in flowrate anymore? Maybe the answer is, that more and more of all efford (and money) has to be invested into finding and taking the energy out of the ground? Maybe one result of my theory is that there is a new tool of measuring the "real productive part" of the share of the GDP which - in real - comes out of the "financial industry"? Ludwig Poullain, former chief of the WestLB said in an interview 2010 "you have to make a difference when comparing 5 billion earned by a car producer like daimler and a bank with 5 billion earned. There is a difference ... " What if my theory can give an answer to these questions? What if 150 years of economic theory are not enough to give all possible answers? What if I am right? What if there is a possibility of really measure the total size of an economy? What if there is a possibility of bindung the floating amount of money to a real measurable size of any given economy? Well. I just think it is worth the discussion and not worthless. And I am happy because I absolutely know that I am right ... ok, maybe I am wrong with this ... by why not thinking about it? So, thanks for your reply! Olaf

FRAP, DPPH, NO are some routinely used assay that can help you.

more or less yes you can find the detail protocol on this paper Samuele Voyron, Sophie Roussel, Françoise Munaut, Giovanna C. Varese, Marco Ginepro, Stephan Declerck, Valeria Filipello Marchisio. 2009 - Vitality and genetic fidelity of white-rot fungi mycelia following different methods of preservation. Mycological Research 113:1027-1038.

If participation reading is a continuous outcome variable and you want to test for mean differences in that variable between your five lists of words, then the latter is your categorical exposure variable and thus ANOVA (or linear regression) would be a suitable model.

NMR, HPLC and GC are some great technique which can help you to identify the metabolite, you can look for chromatography, followed by its broad group characterization.