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Answer added in Drug DevelopmentNew I have general question about the conformation of the drugs in human body?Your question is not clear but as much i can understand you are talking about the predicted docked pose, its binding insilico and its binding with pro... [more]
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Answer added in Plant Tissue Culture5 Is there any protocol for mass multiplication of Stevia (Stevia rebudiana) under in vitro conditions?http://www.academicjournals.org/AJB/contents/2012cont/5Jun.htm Please find the last article in Plant and Agricultural technology section. Regards
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Answer added in Drug Development13 Should serum be added to drug dilutions made in cell culture media?I think it is better to use serum free medium, as the drug may interact with the serum proteins and this may affect the activity of the drug. For me I... [more]
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Answer added in Biotechnology Industry1 Can anybody explain poultry litter for biogas production tips to follow smooth operationFirst and for most the search microorganism which can turn this litter into proteins and amino acids. After this go for methanogenesis
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Question asked in BiogasNew Does anyone know where to find the variation of biogas production by food waste?I would like to know the difference between summer and winter time.Does anyone have or know where to find the variation of biogas production by food waste? I would like to know the difference (in termi of biogas produ... [more]
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Answer added in Cell Culture9 Can anyone please show me how to increase transfection efficiency when lipofectin 2000 was used on the cell line 3T3 or neuronal cell line GT1-7My experience with NIH3T3 transfection is also that lower densities work best for transfection (8000 cells per square cm), or about 25-30% confluent. ... [more]
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Answer added in Myeloma8 MTT assay problem in multiple myeloma cell lines?Thank you very much for your ımmedıate reply ın my ıssue. In each experıment I used dıffrent alıquots of IGF-1, ın order to avoıd freeze-thaw... [more]
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Answer added in Animal Models18 How fast does a drug in solution injected i.p. reach the bloodstream?My experience with i.v. injection in mice is, that is is usually not that easy for most scientists or even rather difficult and needs lots of experien... [more]
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Answer added in Diagnostic Radiology4 MR brain volume spatial normalization?OK thanks but what I was wondering is that if two brains are not perfectly matched which sense has the voxel based analysis? By the way, thank you fo... [more]
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Question asked in Enzyme ActivityNew What are the approaches or softwares i should use to determine which carbohydrate molecules have enzymatic activities?By computation how to find out the carbohydrate molecules which have catalytic activity?
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Question asked in MATLABNew How to present different visual stimulus according to serial input (trigger) received in Psychtoolbox in Matlab?I'm using Psychtoolbox for Matlab for presenting visual stimuli. I need to change the visual stimuli according to a serial trigger received from anot... [more]
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Answer added in Pulmonary Embolism6 How do you follow patients with pulmonary embolism?In the study we are running in Sarajevo, we were able to follow up PE patients after 3 month, and if normalized perfusion was observed and no other ri... [more]
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Answer added in Remote Sensing Applications28 GPS Analysis Software Running on WindowsNDA Professional SW (developed by Galileian Plus) runs on Windows OS and has been validated by Polytechnic of Milan. Results are comparable at 1-2 cm ... [more]
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Answer added in Bioinformatic Software3 I would like to start a company for providing bioinformatics services, if you have experience with that what are the most demanded services?Based in my experience teaching bioinformatics, I think that it will depends of the customer target. For example, for plant biology it is common RNAse... [more]
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Question asked in Yeast GeneticsNew How to perform a PCR based gene disruption, when the target gene is 'oriented with respect to the Crick (bottom) strand' ?I have been trying to generate a Saccharomyces cerevisiae disruption strain using standard PCR amplicfication of the HIS3 cassette followed by recombi... [more]
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Question asked in Soil PhysicsNew What is meant by the avaiable water holding capacity of a soilWhat does the concept of available water holding capacity mean to you, and how do you measure it. This seemingly simple question hides some complexity... [more]
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Answer added in Instructional technology229 Has digital information/communication technolgy increased or decreased learning efficiency in the classroom?I find all the responses interesting. Don Phillip's response on the subject is very enlightening.
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Answer added in UAV1 which controller is used for designing a MAV ?Hey Venkatatakrishnan, Check the attached pdf. Hope it helps
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Question asked in vessel registryNew can a heavy fine on FOC be a solution to increase national vessel registryPage no 700-701
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Question asked in Cognitive RoboticsNew Any vibrator to measure human muscle vibrations in real time?I need to measure human muscle vibrations. Could you please suggest any available high precise one?
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Question asked in General RelativityNew Предлагаются к обсуждению наброски для Общей Теории Хаоса (outline of the General Theory of Chaos)С чего всё началось находится здесь http://www.twinqueens.blogspot.com/2013/04/index.html а к чему всё сейч... [more]
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Answer added in Genetic Diversity17 What software, besides Structure (Pritchard et al 2009), could I use for population structure analysis?Maybe you could have a look at STAGECOACH (Cochran and Ellner 1992)...
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Answer added in Fungal Biology9 I want the fungus cryopreserved at -80 ° C, but I do not know what is the concentration of glycerol used ?You can the concentration ranging from 20 to 50% depending upon the culture
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Answer added in Photovoltaic Systems8 Can solar photovoltaic(PV) technology work efficiently in high altitude mountains ranging from 3000-4000m? Are there any instances of the same?For more altitude better. More solar energy by squar meter
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Answer added in Embedded Systems3 Any suggesions to increase the bit resolution of measurement when done with arduino in millivolt range scale ?If you can live with lowered sampling rates, Marek has already shown the cheapest and most intelligent approach! If you don't mind additional hardwar... [more]
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Answer added in Surface Characterization5 Can microspheres (Latax/Polystyrene microparticles), having amine-modified surface groups, be considered cationic?Thanks again for your answers. I'm planning to purchase those particles and get also an experimental answer to my question.
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Answer added in Discrete Mathematics8 Who knows of an integrator for the general first order ODE which is second order and needs only one evaluation of the equation's rhs per time step?Ulrich, you haven't understood what is the order of a method. The method may yield the exact solution in certain cases, and still be of first order. ... [more]