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Question asked in AcetylcholinesteraseNew What is the best way of keeping recombinant human AChE as long as possible without loosing its activity?I have recombinant human AChE (powder) purchased from a vendor. The vendor's instruction described to store it at -20 C. Would it be OK if I make ali... [more]
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Answer added in Delusion50 What constitutes a "bizarre delusion" that meets Criteria A for a DSM-IV diagnosis of schizophrenia?Pardon my very late entry into the discussion. My pink chicken was distracted by other concerns. While I suppose there is some intellectual fascinati... [more]
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Question asked in Polymer CompositesNew I need some references for PP/PET blend.How much PP can be blended to PET
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Answer added in String Theory12 Do we believe that quarks are elementary, or are they composite?Just as the "physical electron" is an infraparticle and does not correspond to the non-interacting "elementary particle" or field excitation which app... [more]
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Answer added in Molecular Docking8 Should energy minimization and/or charge estimation be done before using mutant proteins for docking?Absolutely yes!
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Answer added in Protein Purification8 how to go about purifying a his-tag protein, that needs a reducing agent, using a Ni column?Thank you all for the answers. I tried washing the column first with buffer that had 0.5M Imidazole. This turned the column very blue. I then washed i... [more]
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Answer added in Molecular Biological Techniques9 Is there a Taq (or other DNA polymerase enzyme) that can be used to amplify large fragments (20 - 30 kb) and does not have 5'-3' exonuclease activity?I've successfully used Sigma AccuTaq LA to make 30kb products from E. coli genomic DNA. It's a mixture of Taq and a proof reading polymerase (as Joach... [more]
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Answer added in Tumor Cell Culture3 Can I store the drug solution made in cell culture media?1. You say the drug to be insoluble in DMSO and soluble in media, wondering why not in water which makes up the media. 2. The media (animal cell cultu... [more]
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Answer added in Statistics41 Can you recommend a free (open) software that has similar statistical analysis to SPSS/PASW?Try OPENSTAT open ware/ free download available
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Answer added in Predator - Prey Interaction4 Are mesopredators always smaller than the apex predator?I already am aware of the definition. All I am asking is whether there are any examples of a system where a mesopredator is larger than the apex given... [more]
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Answer added in Science 2.013 Which online survey tool can you recommend?I think SurveyGizmo is the best online tool out there. You get way more with the free version than any other option. I've used it for academic work, a... [more]
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Answer added in Metabolomics6 What method should I use to stop cellular metabolism and to extract metabolites from cancer cells?Personally, the best way to rapidly quench any samples for any further mass spec analysis is use a dry ice-ethanol slurry. Then dip a tube containing ... [more]
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Answer added in Conventional PCR4 Multiplex PCRThank you guys for you suggestions. I just wonder if i do multiplexing in one PCR tube then how to run gel for the products? I mean, all gene products... [more]
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Answer added in Planetary Science8 Did an asteroid hit the Earth 12,800 years ago?Thank you Andjelka, I have downloaded it and will give it my best attention.
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Answer added in Academic Writing455 Citing Wikipedia?Thanks for the clarification, David.
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Answer added in Graph Algorithms3 Recommendations of papers related to vetrex cover which is also an independent set?That i knew as for a vertex cover it has to cover all the edges
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Answer added in Business Intelligence Tools2 Can someone help me in understanding what is k-means and r-means and their importance in data mining ?K-means is a simple method that is often used to partition (cluster) the data in an unsupervised way. The centers move around over many iterations unt... [more]
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Answer added in Plant Genetics11 Removing PCR inhibitors POST-extraction?I think that problems are not in the methods or sophisticated protocol of extraction DNA. Maybe the problems are in the condition or standarization o... [more]
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Answer added in Plant Tissue Culture13 Can any one tell me about breaking dormency in the caesalpinaceae family of plants?A couple of suggestions to the many already proffered should the problem continue AND should the material in question be viable (many seemingly sound ... [more]
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Answer added in 1H-NMR15 How to avoid a broad signal in H-NMR spectra due to self-aggregation between analyte molecules?enhance dilution of the analyte until 10-5 Molar or use another deuterated solvent as dimethylsulfoxide sometimes its works
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Answer added in Analytical Chemistry Instrumentation52 Can anyone explain when to use an external standard and when to use an internal standard method in HPLC?I found the following resource quite helpful. I had similar questions as yours.
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Answer added in Diagnostic Radiology8 MR brain volume spatial normalization?Well, if the two brains are not from the same subject, then you can't expect the ventricles to align perfectly since their anatomy will be locally dif... [more]
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Answer added in Transfection2 Caco-2 cells transfection method?Which protocol are you using? Is it published?
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Answer added in Molecular Biological Techniques3 RNA isolation protocol from Euphorbiaceae?Dear Valesac, Euphorbia may be pretty tricky due to the milk-like terrible substances that some of these plants have. I recommend to use a technique ... [more]
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Answer added in Spatial Analysis20 How to make soil Maps from point data?Here is another approach for totally qualitative data - where quantification is not possible. Plot the data points - Use ArcGIS or QGIS. Create th... [more]
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Answer added in Statistical Analysis8 I'm doing a research on English vowel sounds.Given your ordered categorical outcome variable, running t-tests will most likely yield biased estimates due to the normality assumptions being violat... [more]
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Answer added in Native PAGE12 Eluting Biotinylated DNA from streptavidin beads without denaturing?I do not think you can elute the biotinylated DNA from streptavidin using just biotin and non-denaturing conditions. However, you can do that if you u... [more]
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Answer added in Plant Biotechnology2 What are Homeologous Genes?In your case that would be two genes from duplicated chromosomes (homeologs). Any gene on one chromosome in the A set should have a counterpart in the... [more]
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Answer added in Animal Models20 How fast does a drug in solution injected i.p. reach the bloodstream?I really do not know how fast Dexamethasone or other drugs may Need to reach their full pharmacological potential, but I could Imagine that even a sat... [more]
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Answer added in Obesity Epidemic131 Is sugar over consumption the underlying cause of the obesity epidemic?In response to Heidi Kocalis: Dr. Lustig does not claim Fructose alone causes obesity. He clearly discusses its relationship to metabolic dysfunction,... [more]