Thanks a lot Geir for your answer and your proposition. I saw the message and I'll visit the website. Best regards

In the Joint Research Centre there is an ongoing project led by Jean Noel Druon aiming to identify the habitat of key marine species, such as bluefin tuna, fin whale and hake, using satellite-derived data of the sea surface in support of fisheries management and control and/or species protection. Check this website for more information: https://fishreg.jrc.ec.europa.eu/fish-habitat In this site you will also find links to related papers. The developed models for bluefin tuna in the Mediterranean are fully operational and JRC provides short-term predictions of feeding and spawning habitats. These predictions are used by national and EU authorities complementary to other information such as VMS data, to control the tuna fishery.

Dear Constantine After external beam RT, and a 6 month ADT, those patients have undetectable PSA and free PSA levels. The testosterone level would be detectable just for a very short time. But remember that level is a level the patient will live with rest of his life anyway and despite presence of native testosterone we don't use lifelong ADT. I would not use injections of testosterone. Many physicians think leukovorin is a chemo agent and indeed some insurance companies list it as chemo because of its concomitant use with 5 FU or concecutive use with MTX. Just FYI, it was because the subjective favorable effects in cancer patients, why my internist colleague tried it in non cancer patients. As you know folinic acid has better CNS penetration and yields higher tetrahydrofolate levels than frolic acid. So in patients with peripheral neuropathy and fatigue it appeared to have favorable effects. But you need to get a cheap source. My experience with leukovorin goes back to mid 1980s when a young woman with a PNET of brain stem developed debilitating fatigue and neuropathy after the 8 in 1 protocol, (unable to walk, play piano, climb up the stairs or button up her shirt). Leukovorin worked like a miracle. She had a completevrecovery within 10 days. I tried to present the case to my senior colleagues Mark Nesbit and BJ Kennedy, without telling anything about the leukovorin or the outcome, they both suggested the use of leukovorin. Apparently it has been used by the people who established the disciplines of pediatric oncology and medical oncology. I thought you would like to know the context

For some of these clients, being asked to focus on breathing only leads to panicked feelings of fear that they may become unable to breathe or swallow. It is often better to get the mind away from bodily sensations.

Fariba, I find out your method of analysis as fantastic approach and thank you for offering me help but on one side the problem is that I am not so into MATLAB, I mean I know some basics but I do not know how to apply those codes. On other side I must perform this analysis and filtering personally because it is my master thesis and therefore I have to know practically all applied methods in order to defend my thesis. Maybe if application of those codes is not complicated (I mean if is it possible to explain it in click-click manner) then I could apply them personally in MATLAB. Thanks. Best, Bojan

I had planned on using my echo Live Scribe pen but am having trouble getting the audio on my new Macbook Pro. Is anyone else having this problem? I spent several hours last week with their tech support uninstalling and installing the software. It still doesn't seem do to everything it should.

Thanks for your feedback. Whilst I understand your point however during my research I found at least to sources [1] and [2] who remarked that there is a difference at least between Cooperation and Coordination. This is the main reason why I got confused. It seems that coordination implies negotiation to resolve any conflicts. [1] http://web.itu.edu.tr/sariel/thesis/sariel_PhD_Thesis_2007.pdf [2] http://books.google.com.mt/books?id=HaS91phGuRQC&pg=PA271&dq=cooperative+coordination+robot&hl=en&sa=X&ei=33afUd-qIejb4QTD5oDgDA&redir_esc=y#v=onepage&q=cooperative%20coordination%20robot&f=false Thanks

I mean after transformation reaction (100 µl of Cells) I grew them in 1ml for 2 hours, then I plated this suspension on LB with amp/IPTG/xgal.

Yes, interesting discussion, but it doesn´t change the fact that crystallite has a clear definition, for which the size can be calculated or estimated by Diffraction techniques. There is often a confusion between grains and crystallite, as it can be the same thing... or not. In conclusion, the definition and clarity can only comes when you specify the technique that is used to examine the material. But back to the question of Shaopeng, there is indeed no problem. He is asking for crystallite size.

So, you need your own mutex semaphore with blackjack and hookers? Thats not unix way.

Dear Jose, I'm a non-physicist, but I know of some physicists who actually question the existence of dark matter - at least in specific conditions. As a physicist, do you really think that gravitational lensing provides "undeniable evidence" of dark matter's existence? As I understand, justification for the existence of dark matter was established within the physics community by the evidence presented of a 'galaxy rotation problem' - that the rotational velocities of test objects within spiral galaxies did not diminish as a function of their radial distance. Afterwards, confirmation was found in evaluations of gravitational lensing - from a discrepancy between the magnitude of distortions identified in background objects and the estimated aggregated mass of compound lensing objects. No requirement for dark matter has ever been identified for a discrete object of mass, such as a black hole or neutron star, or any planetary system. Only evaluations of very large scale, compound objects comprised of many billions of discretely interacting massive objects have produced discrepancies between approximations of the aggregated masses of observed objects and identified gravitational effects. This was noticed early on by some who conjectured that gravity works differently at large scales and proposed changes to gravitational theory to account for the identified discrepancies - with mixed results. As a highly experienced information systems analyst, these problem conditions suggested to me the that there has most likely been errors in the application of well proven (for discrete objects) simplistic methods of gravitational evaluation to much more complex, enormously compound, self-interacting objects. In the brief, informal essay posted to my profile, I've identified formal, published studies that have at least largely addressed the perceived 'galaxy rotation problem' by much more completely representing the actual planar distribution of mass and gravitational interactions found in spiral galaxies. The use both Newtonian and relativistic gravity without modification or compensatory mass. Perhaps we can discuss gravitational lensing - now commonly considered to be the best evidence for dark matter - later. There are additional issues, even for some physicists.

My mistake, it is , of course 800°C and 350°C.

I don't know what organism you are working on, but you would probably have the sequence of the genes ( mRNA ) that you want to test expression on, or you can find it on a database? . You can predict the size of the PCR product based on the location of the primers on the sequence. In fact, if you are performing two-step rt-PCR, I would recommend that detection primers, if possible, generate a PCR fragment spanning an intron, so that you can either distinguish DNA from cDNA amplification (DNA amplified products should be larger), or that only cDNA amplification is possible (depending on the size of the intron).

Old references : A. Bouacha, " Alors dans le discours pédagogique: épiphénomène ou trace d'opérations discursives? ", Langue Française, n° 50/1981 M.-B. Hansen, " Alors and donc in spoken French: A reanalysis ", Journal of Pragmatics, n° 28/1997, p. 172. Fernandez, J. (1994), Les particules énonciatives, Paris, P.U.F. On line Robert Bouchard, 2002, Alors, donc, mais..., « particules énonciatives » et / ou « connecteurs » ? Quelques considérations sur leur emploi et leur acquisition, Syntaxe et sémantique 2002/1 (N° 3), http://www.cairn.info/revue-syntaxe-et-semantique-2002-1-page-63.htm

i think the convention of negative frequency arise simply from introducing Euler's theorem for mathematical simplicity. And for the directional approach, if we start defining the frequency as a vector quantity including direction, like a wheel rotating in anti clockwise direction or clockwise direction, we can think of negative frequency. My idea is that the occurrence of negative frequency will be only in the mathematical world.

The answer by Zaid, especially no. 3 will be main driving for LTE-TDD. As WiMax is going away and all is heading to LTE, data companies, like Sprinit-USA, which were main advocates of WiMax, which is TDD, will go to TD-LTE rather than to FD-LTE to effectively utilize the spectrum previously assigned to their WiMax systems without the need for paired spectrum. Companies which evolved from the 3GPP stream are more slower in heading to TDD mode of LTE. Thanks. @AlDmour.

I don´t think it is necessary as long as your positive results lie within standard range. Do you have any experience about this assay and what values you have to expect? If there are values bejond the 1000pg point you should increase your standard range of course. The positive measurements must lie on the curve so you can be sure your assay works properly. Based on own experiences there are assays that run within a certain "standard window" and lie beyond the curve when this intervall is exceeded. On the contrary, other assays have a much wider efficiency range.

Hi, Jannie. The quote is interesting, thank you. Can you give us the full citation, please?

a proliferation assay with CFSE it´s not too expensive (even less expensive than MTT).

Dear SabitaChaichandran How will you determine vaginal site specific defect for cysto and rectocele? Can you tell me the sensitivities of those procedure?

Correct and being connected to either an AC or DC source matters very little.

I agree with Uttam. Very good explanation. Another cheap method is to use TLC and revealing by a ninhydrin solution. It is ver sensitive for amino acids and cheaper because you don't need a HPLC system/solvents or UV reader.