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  • Answer added in Cell Culture
    22 Which is more economical: coverslips or a specific haemocytometer cover?
    By Triona O'Connell · Dublin City University
    Abdul Waheed · Biocon
    Most of the error in counting is due to floating of the regular coverslip when more sample is injected into the chamber (forceful rather than capillar... [more]
  • Answer added in RNA Analysis
    1 Extraction of platelet RNA
    By wu Weibin · Sun Yat-Sen University
    Darrell Green · University of East Anglia
    What RNA extraction kit did you use? Did you blank the nanodrop with nuclease-free water? 
  • Answer added in Economic Growth
    10 Why using money for "measuring" of any given economy? Why not using a real unit, a physical unit?
    By Olaf Schilgen · Burg Giebichenstein Kunsthochschule Halle
    Peter Stauvermann · Changwon National University
    Dear Olaf, I think you would like to get something like absolute value of something, but that does not exist. All things have only a relative value, a... [more]
  • Answer added in Drug Addiction
    5 Which medication approaches could target the stress response in drug addiction?
    By Wilfrid Raby · Albert Einstein College of Medicine and Columbia University
    Jack Chamberlain · Ivytech Community College
    Admittedly this is not a new idea, but there are a few newer drugs that probably have not been investigated. I would look at drugs that target nucle... [more]
  • Answer added in Conducting Polymers
    3 I am trying to measure OFET characteristics with Keithley 4200 SCS Set up. Can someone please explain me what are Source Current and Gate current?
    By Sujit Kumar · IIT Kharagpur
    Sujit Kumar · IIT Kharagpur
    Hi Martin, Thanks for pointing that out. So Do you mean to say the gate electrode and the drain electrode are somehow connected?But do you notice the ... [more]
  • Answer added in Computer Hardware
    10 Can a rack server run an additional NVIDIA Tesla GPU?
    By Dag Lyberg · University College Cork
    Jonathan Hogg · Science and Technology Facilities Council
    K20 should have EEC too - though you'll find things run faster with it disabled. Depends on how mission critical data integrity is (we've never had a ... [more]
  • Answer added in Animal Physiology
    11 In the case of hypoxia, what gas(es) becomes the dominant species that replace oxygen in the body?
    By Yong Lin · University of Virginia
    John Raphael Almanza · Ateneo de Manila University
    Oxygen is not necessarily replaced. The organism can undergo anaerobic respiration which produces an acid by-product which decreases affinity of hemog... [more]
  • Answer added in Ontologies
    4 How many Business and Enterprise ontologies are there and how do they relate to each other?
    By Wim Laurier · Facultés Universitaires Saint-Louis
    Michael Brückner · Naresuan University
    Your question is about quantity, but I think you actually want to relate to appropriate ontologies. The Gellish project aims at supporting business an... [more]
  • Answer added in Phase Transitions
    2 What is the surest way to distinguish between displacive and order-disorder phase transition?
    By Tapan Chatterji · Institut Laue-Langevin
    Kirill Zhuravlev · Carnegie Institution for Science
    Try Raman, you should see the softening of one (or more) of the lattice modes. Raman can give you decent signal even for micron size samples. Another ... [more]
  • Answer added in Ecology
    2 In mullerian mimicry, why does a poisonous animal copy another poisonous animal?
    By Tomas III Lazatin · Ateneo de Manila University
    Nadine A. Gund · Bayerische Landesanstalt für Landwirtschaft
    Hi, an explanation: The phenomenon can be understood by imagining two poisonous species that do not resemble one another and are also prey to a commo... [more]
  • Answer added in Western Blot
    3 What is the best loading control for membrane fraction western blot?
    By Susitha Premarathne · Griffith University
    Olga Issaenko · University of Minnesota Twin Cities
    You could use ab against integrin or cadherin subunits. Just be carefull when selecting antibody because both have cytoplasmis domains 
  • Answer added in Physical Chemistry
    1 What is the difference between alpha and gamma alumina?
    By Bayu Ardiansah · University of Indonesia
    Shubhankar Bhattacharyya · Defence Research and Development Organisation
    Its differ in crystal packing...of these solids.... 
  • Answer added in Spatial Analysis
    11 What GPS mapping software do you use?
    By Nicolas Scharioth · Pollion - The Smartphone App for Research
    Jennifer Hooper · United States Department of Agriculture
    Since the agency for which I work has a license to the software, I use various programs for developing maps or analysis products which includes ArcMap... [more]
  • Answer added in Migration Studies
    6 Migration research
    Hasan Mahmud · University of California, Los Angeles
    Hi Mary, To ask a question in this forum is easy- on top of the page you see the RG home, you will also see topic, publication, projects, more. Clic... [more]
  • Answer added in Organic Synthesis
    2 How can I perform click reaction of propagyl bromide and sodium azide ???
    By Hussein Hassan · Wuhan University of Technology
    Shubhankar Bhattacharyya · Defence Research and Development Organisation
    this will occure one type of Banert cascade reaction....see this link... http://en.wikipedia.org/wiki/Banert_cascade https://www.thieme-connect.de/DO... [more]
  • Answer added in Analytical Chemistry
    3 What does two value of pKa denotes?
    By Sabin Shakya · Prince of Songkla University
    G V S R PAVAN KUMAR · Maharaj Vijayaram Gajapati Raj College of Engineering
    http://en.wikipedia.org/wiki/Acid_dissociation_constant 
  • Answer added in Animal Models
    6 How fast does a drug in solution injected i.p. reach the bloodstream?
    By Theodore Kapellos · University of Oxford
    Jason Fritz · United States Environmental Protection Agency
    As others have stated above, it depends not only on the drug and vehicle, but volume and frequency of administration. Is this going to be a single-do... [more]
  • Answer added in Medicine
    4 Would like to know what "randomized double blind crossover" means
    By Dani Kamel · Zagazig University
    Sharon Norman · Cardiff University
    Cardiff University provide an e-Learning stand alone Masters level module on Research, Statistics and Evidence Based Practice, which is appropriate fo... [more]
  • Answer added in Cell Biology
    11 portable CO2 incubator?
    By Nelson Ng · The University of Hong Kong
    John Raphael Almanza · Ateneo de Manila University
    There's a cheaper way. I don't if this was suggested already but you can use a jar with a candle inside which would deplete the levels of oxygen in th... [more]
  • Answer added in Protein Phosphorylation
    3 Protease/phosphatase inhibitor cocktail, which is the best?
    By Sérgio Ribeiro · University of Lisbon
    Juan Sironi · Albert Einstein College of Medicine
    I like Halt from pierce it is a x100 solution, you can get the aliquoted in 100ul each single use 24x100ul. It has protease or protease and phosphata... [more]
  • Question asked in Critical Thinking
    New What is 21st Century Literacy?
    I welcome questions and comments about the concept of "21st century literacy." I have written a new open and interactive textbook, which is freely ... [more]
    By J. Beach · University of Texas at San Antonio
  • Answer added in Gender Studies
    22 Gender differences in moral identity?
    By Susanne Täuber · University of Groningen
    Larry Carlson · United Tribes Technical College
    Very interesting Steffen. Study available for perusal? Any speculation as to cause of disparity between follower/leader results? 
  • Answer added in Psychotherapy
    49 Any suggestions for qualitative articles on clients' reports on their experience of individual psychotherapy for a "meta-analysis"?
    By Heidi Levitt · University of Massachusetts Boston
    Sabrina Cipolletta · University of Padua
    You might consider also: Salvini, A., Faccio, E., Mininni, G., Romaioli, D., Cipolletta, S., & Castelnuovo G. (2012). Change in and through psychother... [more]
  • Answer added in DFT
    4 Error in qst2 calculation
    By Shubhankar Bhattacharyya · Defence Research and Development Organisation
    Shubhankar Bhattacharyya · Defence Research and Development Organisation
    thank you Francesca May be u r correct. I dont understand why this occure..here r my error out put GradGradGradGradGradGradGradGradGradGradGradGrad... [more]
  • Answer added in ResearchGate
    13 Should RG provide a nucleus of expert scientists for specific areas that is available for guidance to a budding scientist?
    By Leonard Amaral · Universidade NOVA de Lisboa
    This is a bit more formal and structured interaction then I would be comfortable with, but others can give their opinion. 
  • 99 Is there any free software that can calculate optical constants from thin films reflect and/or transmetance spectra data?
    By Azzeddine Dehbi-Alaoui · Université Sidi Mohamed Ben Abdellah
    M. Kechouane · University of Science and Technology Houari Boumediene
    Dear Junaid Ali, Mohamed El Jouad and Leke Olarinoye I have just send you the program Exoptic.exe, you must first install Visual Basic (VB40) or VB60... [more]
  • Answer added in Cancer Cell Line
    14 Where can I find triple negative breast cancer cell lines?
    By Miguel Gutiérrez · Instituto Tecnológico y de Estudios Superiores de Monterrey
    Piedad Gomez-Contreras · University of Iowa
    Que bueno Miguel, si se te ofrece dime, yo tengo varias triple negativas aqui en EU. MDA-MB 468, Hs578t , MDA-MB 436, MDA-MB 453, MDA-MB 231. Tambie... [more]
  • Question asked in Speech Language Pathology
    New What is the research based recommendation for minimum number of words or utterances for connected speech sampling of child speech?
    100 utterances is the standard LANGUAGE minimum but there are few recent references for how many syllables, words or utterances for analysis of connec... [more]
    By Patricia McCabe · University of Sydney