As Neeraj has suggested, if you use fluorescent nanoparticles, you can use a IVIS camera to detect the fluorescence within the body in vivo without sacrifying the animal. Near infra red dyes are the most appropiate ones in at least in mice since the body does not show autofluorescence at this wave length. We have used this technology with GFP dyeing also but the animals show high autofluresce and you need high nanoparticle accumulation to clearly see the biodistribution.

"...we do not know what thing the universe is. "The world - David Hume writes - is perhaps the rudimentary sketch of a childish god, who left it half done, ashamed by his deficient work; it is created by a subordinate god, at whom the superior gods laugh; it is the confused production of a decrepit and retiring divinity, who has already died" ('Dialogues Concerning Natural Religion', V. 1779). We are allowed to go further; we can suspect that there is no universe in the organic, unifying sense, that this ambitious term has. If there is a universe, it's aim is not conjectured yet; we have not yet conjectured the words, the definitions, the etymologies, the synonyms, from the secret dictionary of God. The impossibility of penetrating the divine pattern of the universe cannot stop us from planning human patterns, even though we are concious they are not definitive." J.L .Borges. http://www.alamut.com/subj/artiface/language/johnWilkins.html. Human patterns: we are working on it. This is the "true" mediated by individual experience and the unity of human experience, if you are believer or not. This is the human experience, this is the mater of Science. YOU need to leave something as a signal of what you have done in your life. Think that God needs the same is a very poor conception of divinity. YOU need to let your fingerprint on whatever you do. Search for fingerprints of God to "'filling the gaps' imposed by the methodological naturalism bounds of modern synthesis" puts god into an arrogant position. Be arrogant. OK. but do not translate your arrogance into an arrogant "Designer" of the world. ID: NO. Tannenberg, 2004 tsunami, the holocaust, etc., are also IDs? Your anthropocentric view of intelligence let the Designer as a very stupid guy. Let's go ahead with Darwin!

The compacting pressure is very low, can't expect great changes in te electric properties

I am a PhD student working at the Microsurgery Unit of the Jesús Usón Minimally Invasive Surgery Centre wich is placed in Cáceres, Spain.

As I understand the computations on elliptic curves are actually much harder compared to other crypto systems, and therefore you need less key bits to get the same security level (e.g., 160bit ECC vs. 1024bit RSA). Depending on the implementation it can still be faster though. But compared to AES, I think it should still be slower by orders of magnitude. So when you encrypt 10,000bit with AES, you can encrypt let's say 1 bit or 10 bit with asymmetric crypto (wild guess). Why would you want to do that?

Dear Dr. M. Kechouane, please send me too the software Exoptic.exe. Thanks in advance. my email ID is anwar_manzoor_rana@yahoo.com

Nice, i got the answer, thank u for your kind reply and finding me the answer.

I guess you are interest to do remote sensing data analysis then you can use GRASS a free and open source software if not openCV or Matlab can be used.

Peter, the best way to be sure that you've got a full length 5' UTR is by RACEing it (is that your oligo capping?). In the old good days of cDNA cloning, people used to work until a full ORF was cloned , usually not being interested in the 5'UTR (trying to obtain longer 5'end sequences was difficult and time consuming). This is why most of the sequences at the GenBank are incomplete by their 5'UTR.

Dear Peter, many thanks. Do you maybe know where I can find a theoretical document about it. Kind Regards Alessandro

Some anthocyanins could be mono- or di-acylated and stability of these compounds strongly depends on pH, temperature and others. It is suggested that "slight" acidification of solvents prevents degradation of these metabolites (Fan et al 2008 Optimizing conditions for anthocyanins extraction from purple sweet potato...)

Dear Nenad, Thank you for this answer. If we speak about permutation ... do you have an opinion about the "what if simulation" to measure the importance ? Vincent

True talk Bhanu, a web search will serve.

Hi Al, Actually, it was Neil who wrote that. I meet people suffering "non-bizarre" delusions all the time. Sometimes they even ride their bicycles to my house, knock on my door, and ask permission to try and persuade me to share their beliefs. Paul.

Hi Qaisra, In general there is a limit to how many passages you can take your cells through. Apparently RAW cells should not be passaged too many times, refer to the recommendations of the cell msupplier. Also, it can take time for any cell line to grow optimally and it can take a number of passages. Why don't you measure the ROS in all your passages. Also ensure that your culture conditions are optimal and just in case check the quality of your LPS. Your ROS measurements must be very high not to find difference with LPS so I would definitely check what passage number gives you the healthiest cells so that you get differential production in response to LPS.

Don't know what you mean by "recent" - this is a feature of essentially all GUTs going right back to the first ones. A simple example of how this works is the (now ruled out) SU(5) model of Georgi and Glashow. This model unifies quarks with leptons. For example (d_r, d_g, d_b, \nu_e, e), the red, green and blue down quarks, and the electron neutrino and electron (left chiral) are grouped together in the 5* rep of SU(5). There are gauge bosons that transform the down quarks into the leptons and vice versa. These gauge bosons are the "big sisters" of the ordinary gluons and weak gauge bosons. They disappear from low energy physics because they get large mass ~10^15 GeV by a simple generalisation of the Higgs mechanism.

Max, thank you for the description. May I ask what the video otoscopy biomarker is? I am glad to see that among various underlying problems, you do find that some factors are as simple as <nutritional deficiencies, dehydration, incomplete or poor quality sleep, and/or environmental/food sensitivities>. Dehydration is of particular interests because it is related to the hypothalamus, a center also for temperature, apetite, and driving (like a reactive rheostat) behaviour and emotions (ettect directly in the brain) -- things that affect women more than men, like FM. To <medicine side effects> and < Medications of that class tend to cause the very symptoms they were meant to relieve once the underlying fators are addressed.>, I would add that our own endogenous 'medicines' can also have these effects (eg pushing stress hormones such as energetic/anti-depressant Norepinephrine too far ends up suppressing sleep, apetite, nutrient processing in cells, and energy production itself).

What mean text belove the question: "I am looking for a low cost catalyst or method"? You are looking for extraction method or catalyst for what? Conversion to biodiesel? Please, be specific with your question, otherwise you can not expect answer... Regarding my answer, using Google with that keywords you can find a lot of information...

@ Constantine "learning a language is much easier when we are young the younger the better" - that's an old prejudice.

We don't work on this plant, but an interestig paper was published on direct shoot bud induction and proliferation from cultured leaf and nodal explants taken from mature plants by Pourvi Jaina, Sumita Kachhwaha and S.L. Kothari (Scientia Horticulturae 119 (2009) 315–319)

Well - in order to stimulate the discussion I like to summarize my views concerning linear/non-linear etc: 1.) Strictly spoken, there are no elements/parts/circuits which really are linear. Linearity is a mathematical term that cannot be applied to real electronics. 2.) However: It is good engineering practice to treat some elements as linear as long as the deviations between linear and non-linear are acceptable and are below the application specific requirements. 3.) Thus, using the term "linear" one should know that the real meaning is "quasi-linear" (or "linear enough"). Otherwise, one always would have to mention the "degree of non-linearity". (As another example: In the field of electronics there is no formula that is correct by 100% - but we use, nevertheless, the equality symbol "="). 4.) In this sense: A linear part (example: resistor) has a static resistance (ohmic resistance), which is identical to the differential (dynamic) resistance of the device. 5.) A part having a non-linear characteristic (example: pn-diode) has two different resistive properties: (a) R(static)=DC voltage-to-DC current ratio (b) r(dyn)=ac voltage-to-ac current ratio (around a fixed dc bias point). 6.) Explanation to 5(b): This is a small-signal value only - identical to the slope of the non-linear V-I characteristic (differential quotient). 7.) Finally (with respect to earlier discussions): The terms linear/non-linear cannot serve as a criterion for the classification active/passive. Lutz vW