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  • Answer added in Western Blot
    2 Unspecific 50 kDa band on Westerns from human tissue
    By Aneta Dobierzewska · University of Kentucky
    Kai Albring · Universitätsklinikum Jena
    Hi Aneta, I also work with proteins with appr. 50kDa and goat anti-light chain HRP give no background for heavy chain. So I would suggest that you us... [more]
  • Question asked in XRD and solid state
    New How do one determine the Wyckoff functions of particle inorganic compound? Say LaF3?
    I want to know the Wyckoff functions of some inorganic compounds. What are all the sources from which i can get it. Is there a method to calculate ba... [more]
    By Suriya Murthy · Indira Gandhi Centre for Atomic Research
  • New Help on formuating the objective function in Genetic Algorithm.
    I am doing optimization using Genetic algorithm on the effect of parameters on the temperature gain in a particular building, that is on the differe... [more]
    By Alex Matofali · Sokoine University of Agriculture (SUA)
  • Question asked in Chemical Application
    New please guide me about the reaction and manufacturing technique of Polyurethane foam using Polyol and MDI
    can anyone guide me about the mixing ratio of Polyol and MDI for the formation of Polyurethane foam(PUF) and Polyurethane coating liquid. 
    By Ashray Gupta · Punjab Technical University
  • Answer added in Carbon Nanotubes
    1 Different texting voltage can affect the value of the resistor ?
    By Ke Li · Zhengzhou University
    Takuji Ogawa · Osaka University
    If the contact is Ohmic, the electrode distance is not within tunneling regime, and the composite does not perform any chemical reactions by passing t... [more]
  • Answer added in Transcriptomics
    2 What work has been done to determine the degree to which any single strand of DNA exhibits complementary subsequences?
    By William Buckley · California Evolution Institute
    William Buckley · California Evolution Institute
    I notice that with a slight alteration of the shustring software (that I have already sent you), the output will be pairs of complementary strings and... [more]
  • Question asked in Embedded Systems
    New What would be the best Closed Loop Control Techniques for autonomous stabilized Quadrotor/Quadcopter?
    As of now I am totally dependent on PID Algorithm to stabilize my Quadrotor. But, it seems very slow to react and solve the errors. To get rid if I in... [more]
    By Bhaumik Khamar · Nirma University
  • 12 which are the better satellites for soil moisture studying in arid zones?
    By Daniel Montaner · University of Chile
    Kariche Jugurtha · Center for Research in Astronomy and Astrophysics Geophysics
    think that ENVISAT good satellite , you will show in good condition ,small perturbation of deformation incha Allah .  
  • Answer added in Materials Science
    1 how can i form NaHTe or NaHSe stable solution?
    By Huang Wang · Shanghai Jiao Tong University
    Takuji Ogawa · Osaka University
    Do you mean reported methods like the following does not work? "Studies on tellurium-containing heterocycles. Part 18. Preparation and structure of 2-... [more]
  • Answer added in Statistics
    28 What is the descriptive power of a model with R-squared equal to 1%?
    By Demetris Christopoulos · National and Kapodistrian University of Athens
    Demetris Christopoulos · National and Kapodistrian University of Athens
    Anyway, @George' s summary is the good way for empirical model-description of the reality: it is so according to the data, but it does not produce a g... [more]
  • Answer added in Embedded Systems
    33 What is the best option for learning and teaching embedded systems; Raspberry Pi or Arduino?
    By Alisson Brito · Universidade Federal da Paraíba
    Bhaumik Khamar · Nirma University
    Go for Arduino. It would be lot more easier to have some hands-on and understand the Controller's functionality. You need to know only C/C++ to code u... [more]
  • Answer added in Wind Engineering
    15 How to do 3D vector addition?
    By Anna Seres · University of Miskolc
    Kariche Jugurtha · Center for Research in Astronomy and Astrophysics Geophysics
    God give you the answer , good day for you madam 
  • Answer added in Plant Biotechnology
    40 Can anyone recommend a method for checking anti oxidant activity in plants?
    By Reby Skaria · Tropical Botanical Garden and Research Institute
    Hakimeh Mansouri · Shahid Bahonar University of Kerman
    In the plant there are two kinds of anti oxidant; anzymitic (such as SOD, PPO, CAT) and non enzymetic anti oxidants (such as carotenoids, tocopherol, ... [more]
  • Answer added in Programming Languages
    219 Which programming Language is best to learn? Java or .net?
    By Radha Jayaraman · Bharathidasan University
    Bhaumik Khamar · Nirma University
    JAVA.. Platform Independence. Open Source. Flexible. Dynamic. #BeOpen&Free Support Java. Learn JAVA. 
  • Answer added in Radar Systems
    1 Has anyone any experience in combined seismological (seismometer)-geodetical (GPS) and interferometric (InSAR Corner Reflector) permanent stations?
    By Zbigniew Kowalski · Państwowy Instytut Geologiczny
    Kariche Jugurtha · Center for Research in Astronomy and Astrophysics Geophysics
    Very good question !!!! : ) 
  • Answer added in Autism Spectrum Disorders
    7 Are guanfacine and/or clonidine useful in the treatment of autism?
    By John Giblin · Baptist Medical Center, Little Rock, AR
    Geir Bjorklund · Umeå University
    See my answer above about autism. It exists better documentation for the use of selective alpha-2 agonists in ADHD than autism. It is a need for furth... [more]
  • Question asked in Bioremediation
    New Scale up Process
    i am doing project in waste water treatment using bacteria in 5L stirred tank reactor. i have to Scale up this process......... what are the parameter... [more]
    By Puhazh Selvan · Anna University of Technology, Coimbatore
  • Answer added in Quantum Chemistry
    34 Can anyone tells me how to reduce time for calculation in gaussian ?
    By Kapil Dhaka · Birla Institute of Technology and Science Pilani
    Takuji Ogawa · Osaka University
    "i still got error by using %nprocshared=4, even i have 4 cores in my system. %mem is working." I guess your Gaussian does not support parallel comput... [more]
  • Answer added in Proteins
    1 TCA protein precipitation and PEG?
    By Francisco Navarro · Universidad de Jaén
    Didier Vertommen · Université Catholique de Louvain
    Dear Francisco, Two ideas: 1) increase PEG concentration until your protein precipitates, spinning it down would allow you to have a pellet that can ... [more]
  • Question asked in Environmental Biotechnology
    New Scale up the process
    i am doing project in waste water treatment using bacteria in 5L stirred tank reactor. i have to Scale up this process......... what are the parameter... [more]
    By Puhazh Selvan · Anna University of Technology, Coimbatore
  • Answer added in Spatial Analysis
    3 Scale in Google Earth
    By Burnie Nawn · Independent Researcher
    Kariche Jugurtha · Center for Research in Astronomy and Astrophysics Geophysics
    yeah it's one of the problems , but i think that the good way to resolve this , is the consideration of Mr Joshua Kent , to use another GIS and to exp... [more]
  • 14 What are the preprocessing methods to enhance EEG data for general purpose?
    Kaleb McDowell · Army Research Laboratory
    You might want to look into some work by Vernon Lawhern: see "Detection and classification of subject-generated artifacts in EEG signals using autoreg... [more]
  • Answer added in Action Research
    7 Does anyone know of experiments assessing bidirectional influences of action on perception?
    By Roy Salomon · École Polytechnique Fédérale de Lausanne
    Jean-Jacques Temprado · Aix-Marseille Université
    Look at these papers: Acta Psychol (Amst). 2008 Oct;129(2):217-27. doi: 10.1016/j.actpsy.2008.06.001. Epub 2008 Jul 15. Non-linear gaining in precisio... [more]
  • Answer added in Academic Writing
    483 How far are scientists willing to go to publish their rejected articles or scientific reviews?
    By Alessandro Diana · Hôpitaux Universitaires de Genève
    Sanjay Mishra · IFTM University
    I am exhausted, and thus stopped to further justify my previous statements. 
  • Answer added in can anyone help me to find out suitable topic on fine motor
    1 Can anyone help me to find out suitable topic on fine motor development on girls student attention , thank you!
    By Maryam Salehi · Urmia University
    Kaleb McDowell · Army Research Laboratory
    I suggest you ask Jane Clark, she is very knowledgable and may be able to provide some advice. 
  • Question asked in has anyone got this paper from ICEM 90 proceedings
    New "design critera of an IPM machine suitable for field weakening operation"
    ICEM 1990 proceedings 
    By Abhishek Agarwal · Bhabha Atomic Research Centre
  • Answer added in Semiconductor
    4 How can i determine the crystal structure nd lattice constants , when XRD fails (very small concentration)?
    By Shivesh Yadav · Indian Institute of Technology Bombay
    S. Pandey · University of Bologna
    I think you should use HAADF-STEM method for more accurate analysis. 
  • Answer added in Gender Studies
    38 Gender differences in moral identity?
    By Susanne Täuber · University of Groningen
    Larry Carlson · United Tribes Technical College
    I am not trying to dismiss all of Elis’s results, of course. The real issue to me is why some people might prefer to believe that male/female brain ... [more]
  • Answer added in Sports Science
    8 Does diagnosis affect therapy treatment?
    By Fred Bagares · Midwestern University