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Question asked in BiochemistryNew Do we have examples of oxidases that donot follow the ping pong mechanism when 2 substrates are present?Generally most oxidases follow the ping pong mechanism, when 2 sustrates are present. Is there an exception to this rule.
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Answer added in Computer Science Education92 What happened with “The Fifth Generation Computer Systems project (FGCS)”?
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Answer added in Sport8 What is the best filter to process accelerometer dataMansour, The calculation of peak power during a jump from accelerometer data could be very sensitive to the inertial characteristics of the task. Ma... [more]
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Answer added in HLA2 What is the best way to set up a distributed simulation with third-part simulators?It is really good! Thanks a lot :)
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Answer added in Thin Layer6 How to clean dust-like pollutant from thin film?Exactly .. just immerse your film in acetone and IPA then wash it with DI water and dry it with Nitrogen or Just use the spinner and ofcourse all of t... [more]
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Answer added in Genetic Analysis4 difference between HLA Allele frequency and HLA phenotype frequencyOk, I got the drift now. Thanks
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Answer added in Cell Culture63 What are alternatives to using gelatin as a coating for adherent cells on glass slides?You could also use tissue culture platic slides. Just cut some directly from culture dishes with a Dremel (I am serious...). Also, if you experience s... [more]
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Question asked in Air QualityNew What is nano electron beam for determine air contaminants?...?
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Answer added in Gravitation7 What is the status of Einstein's equivalence principle?Gravitation is indistinguishable from a force-free state of rest, not inertial acceleration, no matter how local. Falling in a gravitational field, or... [more]
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Answer added in Cancer Biology8 Which specific patient population with prostate cancer should undergo surgery: Are there biomarkers that identify patients who need surgeryDear Fatih: Good questions all. I take them up below: Management of Some Adverse Events from Prostate Cancer Androgen Deprivation Therapy (ADT) 1. ... [more]
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Question asked in AlgaeNew How does Chlamydomonas reinhardtii reacts on an oversupply of oxygen?I have cultivated Chlamydomonas in the dark on acetat with bubbled air. Could there be an growthinhibition because of an oversupply of oxygen.
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Answer added in Organic Synthesis7 What are the methods to deprotect methylene dioxy groupTry to use acids like sulfuric acid ...hydrochloric acid ....in organic solvent
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Answer added in DFT1 What about the way to produce scientific results from the work of my group near the Chemistry Dept. in University of Trieste ?All the NEXAFS angle-resolved spectra contained in those works were computed by using ADF-External (copyrighted), an angle-dependent dft implementatio... [more]
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Answer added in Mouse Models5 T-47D breast cancer xenograft model in SCID mice?I agree with Licun that increasing cell number can help plus the age of the mice, I faced similar problem with our mammary carcinoma model where we fa... [more]
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Answer added in Immunology3 How to test in vitro whether an extract activates immune cells?I think that you could search a cytosolic production of these cytokines by a pre-treatment of cells with brefeldin A, fix, perm and marking by fluores... [more]
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Answer added in LC-MS31 How to determine the concentration of analytes using the LC-MS/MS when there is serious matrix effect?Try to use standard addition method and use LCMsMs system with Q_trap system
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Answer added in Food & Nutrition2 How do I analyse the influence of taste perception / taste evaluation on the relation between BMI and food intake?I think better you try to use the improve of BMI by that has low taste perception around the especific follow up.
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Question asked in EpigeneticsNew Bed to wigHi everybody, does anyone have any tools that converts bed to wig format??? thanks a ton
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Answer added in SYBR green23 Anyone seen qPCR curves like this?Hi Jason. I agree with comment from Roberto Nussenzveig. But I was wonderng, have you checked youre PCR instrument? Do you have an assay you know wil... [more]
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Answer added in Cognitive Neuroscience1 Can one sensory modality perceive a specific aspect/feature of an object/experience which is normally perceive by another sensory modality?There is definitely quite a large body of evidence showing that humans are able to recognise objects they are only allowed to explore haptically when ... [more]
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Question asked in Applied LinguisticsNew Who came up with the term "additional language"?Although the term has been extensively used in Applied Linguistics, I haven't found any specific references on the origin of this notion.
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Answer added in Water Quality3 I want to install a water purification plant for drinking water for an Ashram.Dear Friends, the TDS value is not very high. thus no RO requirement. Use roughing filters with in line coagulation system. the tretment plant would... [more]
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Answer added in Global Health1 How about forming a "Public Health Research Community (PHRC)" on researchgateI think researchgate is doing this job. Through this site we can share ideas and know each other.
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Answer added in Computational Chemistry12 Gaussian program error: "Normal termination" repeatedI'm just curious, is "Normal termination of Gaussian" the only part of your output that's doubled? Are there any redundant parts in the results, for ... [more]
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Answer added in Organic Synthesis2 Is it possible to introduce a aldehyde group by Vilsmier reagent on fused nitrogen heterocylcles or benzazepines? Has anybody tried?Protect your alcohol by willeamson synthesis to protect hydroxyl group by adding carbon atoms
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Answer added in Physical Education1 Is there any possibility for physical education study field to be a crossdiscipline or interdiscipline in higher education?Absolutely, and I believe it already is! Cross-cultural studies, sociology, and psychology (to name a few disciplines) have already been widely integ... [more]
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Question asked in Immunological MethodsNew ELISA Standard Curve InterpretationHello, I wonder if someone can tell me if the standard curve (attached) for a sandwhich ELISA is good to use. It puzzles me because it is the first ti... [more]
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Answer added in Medicinal Chemistry3 How to seperate compound -peptide from the triethyl amine hydrochloride salt in peptide synthesis?Try dissolving it in 0.1% TFA in water more than 3 times and purify using reversed-phase HPLC
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Answer added in Real-time PCR5 How to deal with conflicting results that occur at normalisation to three different reference genes?No problem! We usually test 5 different reference genes (GAPDH, SDHA, HMBS, HPRT1, TBP), but I guess the more, the merrier. :) But of course the sele... [more]
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Answer added in Cheminformatics and Computational Chemistry3 How to use molden interface with Gaussian software09W for getting geometry optimization of two interacting molecules?Do you want to optimize a geometry of the system that consists of two distinct parts, just like a dimer of water? If that's the case, there's no probl... [more]