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  • Answer added in Protein Purification
    1 Necessity of Bradford's standard plot !!
    By Prem Prakash · Indian Institute of Technology Bombay
    Dogan Demircioglu · Eberhard-Karls-Universität Tübingen
    This is an interesting issue...I would say, that the mean of 3 such determinations should be enough. The only reason to do this standard curve is to b... [more]
  • 13 What is "passive" and what "active" in electricity and electronics? Can we make passive elements become "less passive", "neutral" or even active?
    By Cyril Mechkov · Technical University of Sofia
    Gregg Pulley · Piezo Technologies
    Lutz - Correct. I agree for now that it's a sufficient criterion only. To define further would require that we dig deeper into what specifically we... [more]
  • Answer added in Biological Assays
    4 Have you had experiences of dispensing processes affecting your high-throughput screening results?
    By Antony Williams · The Royal Society of Chemistry
    Joe Olechno · Independent Researcher
    @Michael Berger. I agree that people often believe that hydrophobic compounds are transferred at higher concentrations than expected in serial dilutio... [more]
  • Answer added in Philosophy
    76 Does certainty or absolute truth exist?
    Raja gopalan · IBEXI in India
    Jonte, Truth can't be realized through seeking because mind deceives us by perpetuates this seeking, But truth can be realized through direct seeing.... [more]
  • Answer added in Acrylamide Gel
    1 Why do my SDS-PAGE gel have problems to polymerizate?
    By Francisco Navarro · Universidad de Jaén
    Dogan Demircioglu · Eberhard-Karls-Universität Tübingen
    What kind of SDS gels do you use? (8%,10%,12%,...) Depending on this, the volume of APS and its molarity is important to let a gel polymerize complete... [more]
  • Question asked in Cognitive Linguistics
    New Do we really need a 'Cognitive Sociolinguistics'?
    So far, the field of Cognitive Sociolinguistics has remained fairly close to the Cognitive Linguistics side, being very careful to include factors tha... [more]
    By Jeroen Claes · University of Antwerp
  • Answer added in Evolution
    43 Are individuals "better" adapted?
    By Lluvia Flores-Rentería · Northern Arizona University
    I couldn't ask for better proof of my point than the platitudes that have erupted from the bozosphere in response. 
  • Question asked in Applied Econometrics
    New Is it effective to use standardized coefficients in fixed effect model?
    I would like to employ within transformation in panel data analysis. Market Value Added represents dependent variable. Various value drivers (advertis... [more]
    By Wilhelm Lengler · National Research University Higher School of Economics
  • Question asked in Human Factors Psychology
    New Does anyone have any recomendations about heartrate monitors that can measure HRV several days in row. I
    Heartrate monitor should be able detect small chances in heart rate variation. I found one called bodyguard 2, but Im uncertain if it is accurate enou... [more]
    By Petteri Simola · University of Helsinki
  • Answer added in Maternal Health
    7 What dose of metformin do you use in women with PCOS (with and without prediabetes) who wish to conceive?
    By Eliska Potlukova Horakova · Charles University in Prague
    Mina Moeini · Isfahan University of Medical Sciences
    I prescribe 1000-1500mg daily,divided dose for 3-6month.patient before,during and after use should be checked ;liver enzyme,cr,... 
  • Question asked in Protein Analysis
    New BSA std curve with lysis buffer
    from your experience, what is the preferable range of the diluted BSA to be used in order to obtain a linear curve? I am using 2ug/ul BSA diluted in b... [more]
    Quantitation of protein. James E Noble, Marc J A Bailey
    By Maria Chatzikonstantinou · University of Ioannina
  • Question asked in PEG precipitation
    New TCA protein precipitation and PEG
    I have my purified proteins in a solution containing 37% PEG 8000. I tried to precipitate the proteins by using TCA but it does'nt work. Does anyone k... [more]
    By Francisco Navarro · Universidad de Jaén
  • Answer added in 1H-NMR
    17 How to avoid a broad signal in H-NMR spectra due to self-aggregation between analyte molecules?
    By Marius Ciobanu · Universität Osnabrück
    Serhan Turkyilmaz · University of Notre Dame
    I concur with Serge Söntjens. I prefer to add a small amount of methanol (CD3OD) to chloroform (CDCl3) solutions. This works miracles with lipids for... [more]
  • Question asked in Are interested in a cross-cultural research projects?
    New Are interested in a cross-cultural research projects? Are you from former soviet countries, from the European and Asian continents?
    We are currently looking for potential partners for a series of socio-psychological and organizational studies, joining together partners from Western... [more]
    By Ticu Constantin · Universitatea Alexandru Ioan Cuza
  • Answer added in Organic Synthesis
    3 Plz help me !!
    Frank Kayser · Nurix, Inc.
    Buy 2-pentanone (commercially available), dissolve in THF, react with vinylmagnesium bromide (also commercially available), done. 
  • Answer added in Immunohistochemistry
    7 Some of the tissue cores in my TMA cross sections are coming off after autoclaving. Does anybody know why?
    By Michael Kahramanian · University of Sydney
    Margarita Chernova · University of California, Irvine
    Dear, Michael, we have the same problem. I would like to ask Karen or anyone who has suggestion: since the slides are dried at 50C to activate the bon... [more]
  • 9 Why cut-off regularization is not Poincare invariant?
    By Guruprasad Kadam · Physical Research Laboratory
    Zahid Zakir · Centre for Theoretical Physics and Astrophysics
    Planck length as the grav. radius of the particle appears in spherically-symm. solution and the cutting concerns the radial momentum. The grav. radiu... [more]
  • Answer added in Data
    8 Difference between "interval" and "ratio" variable: How to explain?
    By Monika Wahi · Laboure College
    Clerot Fabrice · Orange Labs,
    woops, had overlooked Jochen answer above ! sorry for the spam ... 
  • Question asked in Numerical
    New Help about Julia
    I need help from someone who understands Language Julia [http://julialang.org/]. Why change the matrix B in this code? A = rand (5,5); for i = 1:5 A ... [more]
    By Sebastian Hernandez · Universidad Nacional de la Patagonia Austral
  • Answer added in Medicinal Plants
    1 What compound is responsable of the antipyretic or analgesic activites in medicinal plant??
    By Amel Boudjelal · Université de M'sila
    Jack Silver · Teledyne ISCO, Inc
    Depends. Salicylic acid is one compound with these activities, there are probably others. 
  • Question asked in Numerical Mathematics
    New Help about Julia!
    Necesito ayuda de alguien que entienda sobre Lenguaje Julia [http://julialang.org/]. ¿Por qué modifica la matriz B en este código? A=rand(5,5); fo... [more]
    By Sebastian Hernandez · Universidad Nacional de la Patagonia Austral
  • Question asked in Real-time PCR
    New How can I reduce the Ct value for the desired gene?
    Ct value for internal control is 20 for 18S rRNA but for the desired gene it is 32. I added 2 ugm RNA for RT PCR and the quality of the RNA was good. ... [more]
    By Inamul Kabir · State University of New York Downstate Medical Center
  • Answer added in Algae Biofuel
    2 Suggestions for protocol for extracting oil from algae to produce biodiesel?
    By Yasser Awad · Suez Canal University
    Yasser Awad · Suez Canal University
    Thank you Dr. Sandra for your answer. Could you give me more details. I appreciate your kind help very much 
  • 24 Should undergraduates aim to get published?
    By Jahnekque Blake · McMaster University
    Raoof Mostafazadeh · Tarbiat Modares University
    Siliva, Interesting, my answer has been rated by Muhammad Firoz Khan. But I have prepared the answer to the question raised from the link below, by ... [more]
  • Answer added in Immunology Techniques
    24 What is the best way to separate human granulocytes from whole blood or buffy-coat? How long can neutrophils survive in culture media?
    By Rocco Cantisani · Novartis Vaccines
    Paige Lacy · University of Alberta
    You can also lyse the RBCs after Ficoll sedimentation of granulocytes. Our lab routinely lyses RBCs after collecting the pellet from Ficoll centrifuga... [more]
  • Answer added in Cardiovascular Surgery
    3 What is your first choice inotrope?
    By Mahdi Najafi · Tehran University of Medical Sciences
    Senol Yavuz · BURSA YÜKSEK İHTİSAS EĞİTİM VE ARAŞTIRMA HASTANESİ
    In our current practice, we prefer dobutamine or norepinephrine as a first choice inotropic to support hemodynamics in cardiac surgical patients. The ... [more]
  • Answer added in Apoptosis
    4 Microvesicles vs apoptotic bodies?
    By Jing Xu · BC Cancer Research Centre
    Jing Xu · BC Cancer Research Centre
    I'm reading through it now. Thanks! 
  • Answer added in Satellite Image Processing
    16 What is the best software to implement an image processing algorithm?
    By Saeed AL-Mansoori · Emirates Institution for Advanced Science and Technology
    Venkatakrishnan Muralidaran · SASTRA University
    Matlab can be used ... But for that u need a computer.. But if u go for OpenCV you can program on a uC ... It would be easy ...  
  • Question asked in Finite Element Method
    New How to assign harmonic load of known forcing frequency for harmonic analysis in ANSYS
    FEM harmonic analysis 
    By Pritesh Bansod · IIT Kharagpur
  • Answer added in Gravitational Physics
    268 Can gravitons be discovered?
    By Prakash Kumar · Vinoba Bhave University
    Dear Yurij Baryshev, Sorry for the delay, and sorry to go so far off-topic, but I'd like to respond to statements you made in a previous response to m... [more]