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Answer added in Molecular Biological Techniques11 Is there a Taq (or other DNA polymerase enzyme) that can be used to amplify large fragments (20 - 30 kb) and does not have 5'-3' exonuclease activity?Hi Fernando, I would definately look at the enzymes from Kappa Biosystems as was mentioned in a earlier post - we have found these ezymes to be excell... [more]
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Answer added in Organic Synthesis1 Hi everybody, how to prepare 3 hydroxy 6 methylheptanoic acid starting from a structure with five carbon atoms ????Add the Grignard reagent derived from Me2CHCH2CH2Br (5 carbón atoms) to 3-Oxo-propionic acid methyl ester (hope the enolic form is not a severe probl... [more]
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Answer added in Sport4 What is the best filter to process accelerometer dataThank you dr jim
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Answer added in Evolution47 Are individuals "better" adapted?By the way, Richard, I note that Jon makes reference to a book that attempts to show how intelligence evolved intelligence, so the question clearly is... [more]
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Question asked in Drug DevelopmentNew Compound intake: Adherent cell Vs suspension cellIf everything else is equal, which cell type will intake compound (in DMSO) faster and retain higher concentration in cell? or there is no significant... [more]
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Answer added in Staining2 Can I use Eosin as a counterstain for Sudan Black?Hello Gaber, I am trying to identify patches of brown adipose tissue in non fixed frozen cryostat sections. I have prepared Sudan Black saturated in... [more]
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Answer added in Planetary Science11 Did an asteroid hit the Earth 12,800 years ago?Using Google you may found about 300 000 interesting articles on the question. See, please, “Comprehensive analysis of impact spherules supports the... [more]
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Answer added in Natural Sciences249 Philosophy and Science, what is the connection?
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Question asked in Proton and electron conductivityNew How can we measure proton and electron conductivities for polymer electrolyte membrane for fuel cells?How can we distanguish between protn and electron conductivity in polymer electrolyte membrane for fuel cell.
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Answer added in Cell Culture59 RAW 264.7 culture issuesWe also use RANKL from R&D (cat. #462-TEC-010/CF) at a concentration of 20ng/ml. You even get nice osteoclasts at 10ng/ml if you don't seed the cells ... [more]
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Question asked in Flow CytometryNew Are there any good markers for cell cycle for use in flow cytometry?I would like to use a DNA-independent marker to tell me what stage of the cell cycle my cells are in at the time of flow cytometry. I have read a few ... [more]
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Answer added in Plant Genetics15 Removing PCR inhibitors POST-extraction?Hi, What you are experiencing is not uncommon in the plant world as the post above attest to. It is most likely your contaimination is due to the pres... [more]
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Answer added in Pulmonary Embolism9 How do you follow patients with pulmonary embolism?A therapeutic strategy based on imaging is intriguing, provided that it has been formally validated. However, a couple of issues should be considered... [more]
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Answer added in Myeloma10 MTT assay problem in multiple myeloma cell lines?Heya, the inconsistency in expt may be due to reason that when you aspirate the supernatant you are loosing some cells. I have used this assay for sus... [more]
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Answer added in Molecular Docking12 Should energy minimization and/or charge estimation be done before using mutant proteins for docking?Sounds reasonable. The foldX solution proposed above (or modeller) seems also good ones to me. Whatever makes your job simpler I would say.
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Question asked in 16th Century HistoryNew Download ots copdaqui bookVicerregal in america
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Answer added in Clinical Biochemistry6 How to process urine samples for storage?Is useful the use of 0,01 % NaN3 to prevent bacterial and micotic growth, than to store the samples at least - 40°C
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Question asked in Real-time PCRNew How to deal with conflicting results that occur at normalisation to three different reference genes?I am currently calculating relative expression values "by hand", using the following equation: Ct(1+Efficiency)^(-Ct). I then normalize these data to ... [more]
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Answer added in Fluorescence Spectroscopy7 Have you ever seen the error message "overflow" during a spectrofluorometer analysis?No, vice versum. Excessive illumination in not possible in UVVis spectroscopy. I think you are just getting 3A+ absorbance. Try dilution, recheck wave... [more]
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Answer added in Bioinformatic Tools4 Can anyone please suggest me a pattern recognition software to identify the 3'UTR regions?I've used Daniel's method before and it works perfectly :) good luck
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Answer added in Liquid Chromatography4 How to efficiently clean a GFC polymethacrylate column after noting the backpressure increased?Dear Matteo Glad to hear DMSO helped.This is the a first step to get rid of the proteins and possibly most of the biological contaminants. Now the qu... [more]
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Answer added in Logic Programming9 Is there any logic language to implement quantum programming?maybe the link below can help you: http://www.quantiki.org/wiki/Quantum_Programming_Language#Quantum_Computing_Language
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Answer added in Higher Education Research98 How do we encourage students to produce more interesting/engaging presentations?Providing access to great examples is a good place to start. There are some great tools out there that do fantastic things and use these to inspire yo... [more]
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Answer added in Bone Research20 Bone Composition AnalysisIt's been a while, but if you are still following this question i can only recommend NMR to you. It can provide quantitative data on bones (compositio... [more]
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Answer added in Oral, Head and Neck Cancer23 Why does oral cancer affecting non-smoker females have a worse prognosis than that affecting smoker males?Dear Tatini as many studies has been done on HPV in relation to OSCC lt they find no significant relation to smoking and alcohol consumption so Ithin... [more]
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Answer added in Reproductive Biology14 What is the physiological definition of "sexual maturity" in fish?Also physiologically the fish can be said to be sexually mature when there is a significant switch of energy allocation from somatic growth to develop... [more]
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Answer added in Science 2.014 Which online survey tool can you recommend?I have used a number of tools including surveymonkey to zintelligence, surveygizmo and qualtrics. It depends on the skills of your survey designer and... [more]
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Question asked in rainfall harvestingNew Rainfall harvesting in arid zonesI am looking for a new approach to rainfall harvesting in arid zones using new technologies such as Nano. Is that any experience in this field?
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Answer added in Psychobiology3 How is clinical depression different from clinical anxiety?From a slightly different perspective, The involvement of limbic - cortical regions of the brain is indicated. The uncoupling of the amygdala from emo... [more]
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Answer added in Molecular Docking3 Can you recommend a docking software able to include structural water molecules?Thanks for your answer. I have done redock implementing a water molecule into the binding cavity, but once I check the docking pose, it can be seen Au... [more]