Topic: water resources management

I use MBP tag to promote the soluble expression of our interesting protein which cannot expressed in E.coli fused with His6 and GST tag. Now, we can obtain the soluble MBP fusion proteins, but it is difficult to perform further purification. There were always some contaminated proteins which were GroEL and single MBP and maybe other proteins identified by MS. So in my opinion, this means the fusion protein maybe form soluble aggregate or at least they didn't fold perfectly. My questions are as following: 1, How can I confirm this assumption? I use sepharose 200 column to determine the molecular size of fusion protein. The apparent size is obviously larger than estimated size, but the estimated size is premised on global protein. so are there some other methods which can determine its aggregated state? 2, if the fusion protein did not fold properly, are there some more efficient strategies to optimize its expression and folding. we have tried different induction conditions such as different temperature and different IPTG concentration and induction time, we use DH5a and Bl21 to perform the expression, they all doesn't work. Others, the main function of this protein is to perform protein-protein interaction, so the activity is hard to confirm.

What are the new trends in control systems for phd ?

I was extracting DNA from Paraffin slides, and after adding xylene, a thick viscous liquid formed on top of the solution. So thick I can't pipette! Any ideas of what it could be?

This may be tricky. I know, that the typical approach is to make a crystalline derivative of an enantiopure compound and then using the typical crystallographic methods determine the absolute configuration. Yet, in my case, when working with specific non-proteinogenic amino acid derivatives this poses a problem, as getting a crystalline derivative seems impossible. I heard, that there is a possibility of establishing the absolute configuration of such molecules via CD or other spectra, via their comparison with modelled data. This would suit my needs, yet there seems to be not much data on the topic. Can anyone help me with that? What kind of equipment is needed, in what software should I build the model?

We are observing two different species populations recovering from disturbance. Both populations were reduced by about 80% with significant changes to their size structure. Following the disturbance, one population's size structure immediately returned while abundance remained low. The second population's abundance immediately returned while the size structure was unexpectedly different (shifted from a bi-modal to a uni-modal distribution). I am searching for some explanations for these different recovery patterns. Any help would be very much appreciated. Thanks!

I interested in some extract to apoptosis pathways in colon cancer cell line?

Phenolics, saponins, other glycosides

For many organizations, sustainability reporting, as gauged by GRI reporting requirements, is now the norm, yet a great number of them go about developing these reports based solely on GRI guidelines. Do you think that organizations could enhance or even facilitate such reporting with the aid of specific elements of ISO 14001?

I'm working in GRASS-GIS and need some help I have to analyze 10 years of MODIS images by using the product -MOD13Q1 NDVI. I understand this band have all pixel already with values of NDVI calculated. So, to get this band I used MRT to reproject the files into Geo-Tiff format. a.- Is it possible to use these Geo-tiff to analyze NDVI for every month in a full 10 years? Also, the region/area downloaded is just one tile, and the area to be analyzed is smaller (crop-land). I guess I will have to specify the geographic area. b.- would someone guide me in this process? by choosing pixels? or selecting a square inside the tile?

I have several Bt isolates which identified as Bt sphaericus. I try to conduct a bioassay on mosquito larvae. Is there anyone can provide me any detail method (density of bacteria, amount of larvae, parameter of obsevation, etc) which can be used to determine the toxicity of the isolates? thanks before.

I tried in Spdbv and VMD, but could not find.

I'm looking for publications about the influence of relative embedding depth on reading times. By relative embedding depth, I mean, for example, the change in embedding depth from word n-1 to word n. An alternative approach would be the number of opening and/or closing brackets. For my work, I want to generate hypotheses for reading corpus studies.

Now, I'm trying to finding some software that can serve me to analyze: - protein - protein interaction - drug (known structure) - protein interaction - drug - drug interaction Can you recommended a free software for this?

I know some of the methods like, Markov chain, Monte Carlo Markov Chain, using some probability distribution but i am looking for a metric which can simulate the variability more accurately.

Does harmonic filter parameters depend on resonance conditions due to harmonics(in a trans line where capacitors are used to neutralize the lagging pf).....or is it designed for frequencies just above fundamental to avoid harmonics and has no relation whatsoever with the resonance condition ....?

For non-native speakers it can be easier to write in their mother tongue. Moreover, pride in ones language can play a role. However, the language of science is English and writing science in other languages has, therefore, drawbacks. I wonder how this issue is navigated internationally.

Our means of perceiving reality through our senses make us vulnerable to distortions and biases. But with scientific methodologies, can we claim that perception of objective reality is indeed possible? OR objective reality only in the context of known knowledge of the time/period? thoughts!

I'm synthesizing some supported catalysts batches with silica and alumina support. Support particles are in granular shape. The final catalysts should be ground into powder form prior to loading into the reactor. I was wondering if there is a significant difference or any preference between 1) First grinding the granular support, followed by impregnating and drying and 2) Impregnating and drying, followed by grinding.

I am using Gaussian for molecular optimization and energy calculation but now i want to learn how to do the same for dimer. In case of dimers orientation and different types of interaction plays a major role in optimization of system, so how to deal with all these parameters ?

Next week, we'll be holding the Second Workshop on Fine-grained Visual Categorization (FGVC^2, see http://www.fgvc.org) in conjunction with CVPR'13. We're in the process of collecting a list of questions for our panel of top computer vision researchers. What questions regarding fine-grained recognition would you like to have answers to? Thanks and hope to see you at the workshop!

I am searching for a paper that contains the origin of an approach. but when I searched in internet, I couldn't find it as PDF format. What is the solution to access papers like this? Carper, W., Lillesand, T. & Kiefer, R. (1990). The use of intensity-hue-saturation transformations for merging spot panchromatic and multispectral image data. Photogrammetric Engineering and Remote Sensing, Vol.56, No.4, (April 1990), pp.459-467, ISSN 0099-1112

All guides for hypertension make efforts to limit salt intake, but really do we know how much they daily take?

One of the many ways to synthesize silver nanoparticles is to reduce AgNO3 with NaBH4. However, the final product we obtain from this reduction process is a yellow colloidal silver. Is the yellow colloidal silver itself ok for direct analysis of silver nanoparticles (UV-vis , SEM, etc) or should I actually further process the yellow colloidal silver by centrifugation / drying in oven to filter out other chemicals present in the yellow colloidal silver in order to get pure silver nanoparticles (and then resuspend it in DI water again)? Does anyone have the exact protocol of the above mentioned steps? I am quite clueless on the sample preparation part for instrumental analysis. The chemical equation for this reduction process is : AgNO3 + NaBH4 ----> Ag + 1/2H2 + 1/2B2H6 + NaNO3 So is it correct to say that centrifugation and drying are needed to remove 1/2H2 + 1/2B2H6 + NaNO3 ? Will centrifugation and the drying process actually lead to the agglomeration of the silver nanoparticles? If yes, is there any way to avoid it? So far, in most of the journals that I've read, not many actually mentioned about the further processing steps that i mentioned above.

While Wikipedia is more and more popular with students, professors discourage them from using it and bar them from citing it. What are reasons (to cite or) not to cite Wikipedia? Jul 31, 2012

There seems to be a standard range of the level of organic matter in the soil in order to refer to a soil as fertile. For instance, according to Metson (1961) as adapted from Booker Tropical Soil Manual (ed. Landon); the level of organic matter of 17.2-34.4 in soil was considered high. Is this classification regional, continental or does it depend on ecological zone?

I have a cytidine•HCl analog, and would like to do some chemistry on it, but the HCl is limiting my solubility in organic solvents. I would like to remove the HCl, but the kicker is the subsequent reaction must be done in the absence of water. NaOH will give me NaCl, but then I would need to remove the salt from the solution. AgNO3 precipitates AgCl, but leaves the NO3 anion. Please note, this is a cytidine analog that is not commercially available, so purchasing it without the HCl is not an option.

I am synthesizing {001} facets films on Ti-Sheets by hydrothermal route. Can anyone please tell me how to BET of my prepared samples?

What is consciousness? Is it something that can be defined purely physically, is it something that does not yield to a physical explanation, or do you have an alternative way of explaining what consciousness is?

I have seen many articles say that the FWHM gives you an indication of the size distribution, but I can't see how they have been calculated. They often just state that a FWHM< xx nm (depending on material) means that there is a tight size distribution.