It becomes grey quickly really - as I see it. As it is really the persons cultural/social group that is of import here. There may be very few of a subculture or culture who hold a view. Unless it is impacting daily life in some negative way I am not sure I would worry about the tiny chickens (I am am color blind) no matter the color. No matter how bizarre/possible it seemed to me. I am not the arbiter of possible - I hope. The Zietgiest changes and takes with it the definition of possible.

I agree with Mark about your low binding problem, if the imidazole concentration was too high your protein would not bind at all. It could be that you did not use enough resin, but it has a very high binding capacity so unless you are loading 20-40 mg of "His-tagged protein" per mL of resin, that is not the issue. Try doing a batch binding for 1 hr at 4oC with gentle rocking to keep the resin suspended, then gently centrifuge the lysate/resin to collect it and then either wash it in batch mode with wash buffer (but will wash away protease inhibitors too), or resuspend resin in a small amount of lysis buffer with PIs and load onto a column for washing and elution. I have found with Talon resin and my "current" protein that I need to do a batch binding to get good binding. Using more resin than needed only increases non-specific protein binding.

Factor Analysis is a descriptive technique so why bother about distributions ? The only possible problem could arise by the sensitivity of correlation (covariance) to outliers but this can prevented by a standardization approach or by log transformation.

Eulemur rubriventer also eat Chinese guava in Ranomafana National Park (Overdorff 1991; Tecot 2008).

Lithium perchlorate in diethyl ether is also popular.

a little expensive but absolutely amazing to get rid of this fatty layer is to use the Columns from the RNaesy minelute cleanup kit from qiagen. You just put your samples on the column and you centrifuge at max speed for 1 minute. Again, it is a expensive but really efficient.

In most cases, to make ultrafiltration membranes, acrylonitrile copolymers are used (maleic acid, 1-methyl-pyrrolidone, polysulfone, acrylamides, e tc.) or in mixtures with other polymers such as chitosan or polystyrene.

I agree with Maria. I think if you meet a problem just after mix everything even RNA or DNA, it's also possible to store your preparation about one to 3 hours in the freezer until your resolved your problem like disruption of machine or courant failed. But if you want to go quickly because you run a lot of RT-PCR, you could pepare mix with all ingredients without DNA polymerase and conserve at -80°C. When you are ready, you defreeze the mix just add the DNA pol and dispatch in the well of plate and RNA.

Sydney, you raised a very important point about the "vitamin E" studies that reported a slight increased risk when taken as a dietary supplement by tobacco smokers. However, as you accurately point out, the "vitamin E" used in these studies contained only one of the eight isomers of tocopherols and tocotrienols that constitute natural vitamin E. I would urge anyone interested in this subject to read both of the recent edited works on tocotrienols to understand their importance to human health: Tocotrienols: Vitamin E Beyond Tocopherols. Watson RR, Preedy VR. [eds.] AOCS Press: Urbana, IL and Taylor & Francis/CRC Press: Boca Raton, FL, 2009; and, Tocotrienols: Vitamin E Beyond Tocopherols. Tan B, Watson RR, Preedy VR. [eds.] AOCS Press: Urbana, IL and Taylor & Francis/CRC Press: Boca Raton, FL, 2012. The mitigation of the progression of pancreatic cancer studies in humans funded by U.S. NIH and reported in the 2012 work is particularly important to read as it suggests that alpha-tocopherol when taken alone may actually block the inherent anti-cancer bioactivities of two of the tocotrientols found in vitamin E, especially derived from a fruit also found in the Amazon, and now available as a dietary supplement ingredient. The 2012 work is a reporting of the second international conference on tocotrienols held in late May 2012 in Long Beach, California. The advances made in our understanding of the characteristics, properties, and bioactivities of tocotrienols is impressive and just starting to appear in discussions among vitamin E researchers who in the past have focused wholly on the tocopherols.

I use my own gel, and my own blotting stack and i have had no problems so far. The transfer seems comparable. I usually use the 7 min mixed MW transfer protocol. The transfer buffer i use is, Bjerrum and Schaefer (0.1% SDS). The tricky part is to replicate the blotting pads/paper. I had tried different kind of filter papers and non of them seem to work. So we switched to a material, which is more fabric than paper, with similar absorbing capabilities.

Sounds like your protein is aggregating. You should know the concentration after purification, use OD or Bradford. Just check now to see what the OD or concentration of the top (soluble) fraction. Also suck off the soluble fraction and transfer to a new tube. You say the band was thick, so you may have a very concentrated solution of protein that just can't be stored for long period of time. Take off what is still soluble, as the aggregated fraction can and will promote further aggregation of the soluble protein. Its also possible you have degradation and proteases present. Did you use PMSF and complete tablet in your purification? IF not you need to!

Wow, thank you for bringing that to my attention! I believe carbonic anhydrase naturally contains zinc. I'm now wondering how others who worked on my project previously avoided this.

Hi Noha, you can use this website: http://eosweb.larc.nasa.gov/cgi-bin/sse/interann.cgi

Majid is a PhD student. I presumed like many PhD students he has little time and/or funding, so Rb-Sr could work out easier, cheaper and faster than Ar/Ar.

That is great. Sound like you have an unique opportunity to examine several key factors together, which has not been possible for many other situations where we can only speculate because the data are not available.

I recommend you the use of geostatistical tools -estimation like kriging or simulation-. For this type of applications (ph mapping), I prefer conditional simulation geostatistical techniques that preserve the variability of the original data (the conditional simulation preserve the min, max values, the mean and variance, the histogram and the spatial variability expressed as variogram fuctions). You can apply them using free software tools like Gslib (http://www.gslib.com/) or the mGstat (a geostatistical Matlab toolbox) http://mgstat.sourceforge.net/. I hope that this recommendation will be useful for you.

I don't think justifying "why" the sampling was non-random is enough unless it is also possible to quantify and correct the bias that is transferred from the non-random sampling design to the statistical results. Sampling method determines the appropriateness of the statistical analysis, and when ignored can lead to grossly erroneous results. A transparent presentation of the non-randomness followed by a coherent and reliable analysis that corrects for the bias or quantifies the uncertainty generated by this bias is necessary for publication. If the bias is unavoidable, then specific statistical sampling designs need to be developed that include necessary auxiliary data to quantify and correct for the bias. The statistical sampling literature is full of examples including size biased sampling, cluster sampling, sampling with detection bias, non-response error etc. In general: Precision of estimation can be improved through increased sample sizes, but only correct and representative sampling can assure accuracy.

I agree Pierre and Dorel. As for Maude I would suggest the Primer (http://maude.cs.uiuc.edu/primer/) as a first introductory text/tutorial. Then you could go to the slides Narciso's introduction to Maude (http://maude.cs.uiuc.edu/talks/maude-padl10-slides.pdf)

A couple of additional options of free GIS software Kosmo: http://www.opengis.es/ gvSIG: http://www.gvsig.com/welcome?set_language=en I hope that these options will be useful for you.

Which compound are you derivatizing? Do you need the derivatization step?

P-type ZnO can be obtained by Sb doping. This information is in next paper.

Most of the time CVP is useless, it is particularly evident when you compare CVP with SVV in a particular clinical scenario.