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• Gutierre Andrade in Wireless Sensor Network

  Personal I need a help, anyone have any WSN simulation module?

  I need to experiment with simulations, the simulator installed OMNET + +, if anyone has any code of a network simulator for this please send me (email: gutierreandrade@gmail.com). thank you
• Theia'a Al-Sabha in Chemistry

  Is their a specific reagent for spectrophotometric determination of arsenic ?

  All the published spectrophotometric methods for determination of arsenic depend on the synthetic samples and the used reagent reacted with other metal ions which present in the sample solution suchAll the published spectrophotometric methods for determination of arsenic depend on the synthetic samples and the used reagent reacted with other metal ions which present in the sample solution such as river, sea and well waters. Therefore, I search for specific reagent for determination of arsenic.
• Andy Greenspan in Neuroscience

  Blatant Falsification of Data in a CELL paper! Has anybody seen this yet? Not much is being done about it within the group that authored this paper. What do you think aboutBlatant Falsification of Data in a CELL paper! Has anybody seen this yet? Not much is being done about it within the group that authored this paper. What do you think about this? http://autismresearchers.wordpress.com/2012/05/09/image-data-falsification-in-a-cell-paper/
• Thomas Weidinger in Cancer Biology

  Specific tumor antigens of pancreatic adenocarcinomas?

  Does anybody know specific tumor antigens of pancreatic adenocarcinomas that could be used for active immunotherapies? I know that there are trials using MUC-1 and CEA. Is there experience withDoes anybody know specific tumor antigens of pancreatic adenocarcinomas that could be used for active immunotherapies? I know that there are trials using MUC-1 and CEA. Is there experience with further antigens of pancreatic cancer? And if not, which techniques might be used for isolating such antigens?

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• Joseph Arul in Educational Management

  What are the factors that constitute personal effectiveness in the context of educational leadership?

  In other words, what competencies are required in a person who wants to be an effective leader in education?

  Recent replies ⋅ Show All (1)

  • 
    Paul Dorra replied

    I think this mainly depends on the modalitys. A relation between one teacher and one student may be quite different to one teacher and a class of students. In both cases empathy is sure one keyI think this mainly depends on the modalitys. A relation between one teacher and one student may be quite different to one teacher and a class of students. In both cases empathy is sure one key competence. Moderational instructions that lead to a learning situation in which the teacher vanishes behind the educational "stuff" seems to me to be another key factor. I hope a wiser person can break down my unsophisticated statement.

    35 minutes ago
• Joseph Arul in Knowledge Management

  What are the factors that constitute personal effectiveness in the context of educational leadership?

  In other words, what competencies are required in a person who wants to be an effective leader in education?

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  • 
    Joseph Arul replied

    I have now posted this question under Educational Management. The question may, therefore, be deleted from the Knowledge Management section. (I do not know how to delete it!)

    2 hours ago
• Denis Solomons in Carbohydrate Chemistry and Glycobiology

  Do you think that an insulin pill could become a reality ?

  An insulin pill would be wonderful for type 1 diabetics or for brittle diabetics who are dependent on insulin. I am sur that Novo would be interested
• Thomas Wong in Methods

  Size Bias in Minipreps - Which method is best?

  I am doing high-throughput sequencing of a library of barcoded plasmids in E. coli. The experimental plan is like this: -grow two populations of cells, one control and one experimental (say, addingI am doing high-throughput sequencing of a library of barcoded plasmids in E. coli. The experimental plan is like this: -grow two populations of cells, one control and one experimental (say, adding a certain metabolite) -isolate plasmid DNA from each population -mix and multiplex sequence the barcodes using Illumina HT-seq The basic idea is that the prevalence of certain barcodes indicates that cells containing those plasmids are more prevalent in the population, and therefore have a faster growth rate. It's therefore important that the population of the cells be accurately represented in downstream purification steps. For this reason, at each step the DNA should accurately reflect what the population of cells was like. This also means that the protocols used to isolate, purify, and amplify DNA not have any size or sequence bias. My library currently consists of members anywhere from 4kb to 11kb, but mainly between 4kb and 7kb. What methods are best for minimizing size and sequence bias? In our lab we use Qiaprep columns from Qiagen for routine work - does this have a size bias in the 4kb to 7kb range? Does alkaline cell lysis followed by ethanol precipitation have a size bias? Are there any other methods that would work for this application? Thank you!
• Thomas Wong in DNA Isolation

  Size bias in Minipreps - Which method is the best?

  I am doing high-throughput sequencing of a library of barcoded plasmids in E. coli. The experimental plan is like this: -grow two populations of cells, one control and one experimental (say, addingI am doing high-throughput sequencing of a library of barcoded plasmids in E. coli. The experimental plan is like this: -grow two populations of cells, one control and one experimental (say, adding a certain metabolite) -isolate plasmid DNA from each population -mix and multiplex sequence the barcodes using Illumina HT-seq The basic idea is that the prevalence of certain barcodes indicates that cells containing those plasmids are more prevalent in the population, and therefore have a faster growth rate. It's therefore important that the population of the cells be accurately represented in downstream purification steps. For this reason, at each step the DNA should accurately reflect what the population of cells was like. This also means that the protocols used to isolate, purify, and amplify DNA not have any size or sequence bias. My library currently consists of members anywhere from 4kb to 11kb, but mainly between 4kb and 7kb. What methods are best for minimizing size and sequence bias? In our lab we use Qiaprep columns from Qiagen for routine work - does this have a size bias in the 4kb to 7kb range? Does alkaline cell lysis followed by ethanol precipitation have a size bias? Are there any other methods that would work for this application? Thank you!
• Angom Lenin Singh in Genetic Engineering

  Is anyone have a copy of the following paper ?

  Is anyone have a copy of the paper "THE ESTIMATION OF GENE FREQUENCIES IN A RANDOM-MATING POPULATION" by R. CEPPELLINI, M. SINISCALCO and C. A. B. SMITH. Please kindly do coperate to my researchIs anyone have a copy of the paper "THE ESTIMATION OF GENE FREQUENCIES IN A RANDOM-MATING POPULATION" by R. CEPPELLINI, M. SINISCALCO and C. A. B. SMITH. Please kindly do coperate to my research work. Looking forward to your invaluable support. Thanks Lenin Singh. (angom.lenin@yahoo.in)

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  • 
    Angom Lenin Singh replied

    I sincerely appreciate you Mr. Catalina for your loyalty. Thanks a lot. God bless you. thanks once again

    2 hours ago
• Jon Moulton in Muscular Dystrophy

  Gene Therapy for Muscular Dystrophy: Lessons Learned and Path Forward. Mendell JR, Rodino-Klapac L, Sahenk Z, Malik V, Kaspar BK, Walker CM, Clark KR. Neurosci Lett. 2012 May 17. [Epub ahead ofGene Therapy for Muscular Dystrophy: Lessons Learned and Path Forward. Mendell JR, Rodino-Klapac L, Sahenk Z, Malik V, Kaspar BK, Walker CM, Clark KR. Neurosci Lett. 2012 May 17. [Epub ahead of print]

  http://www.ncbi.nlm.nih.gov/pubmed/22609847
• Salman Beg in Programming (Java, C, C++, C#,...)

  How to handle a single player game with computer using C program.

  I want to write a game program by using one player means where a user can play the game with computer.For which i have tried a lot but i can't find any type of solution regarding it. So, pleaseI want to write a game program by using one player means where a user can play the game with computer.For which i have tried a lot but i can't find any type of solution regarding it. So, please provide me some of the logic for which i can implement it in my program.Thank you.

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  • 
    Jimmy Allen Davis replied

    Not sure I'm following. Do you want to write a full game? Like CoD, MW3, or WoW? What exactly are you trying to do?

    3 hours ago
• Rick Anthony Ricketson in MicroRNA

  I have a potential novel miRNA from ebolavirus. Suggestions as to how this may be processed in the cytoplasm?

  5' UTR of the nucleoprotein gene (genomic) from EBOV (Pandora Element) showing miRNA precursor (ab initio). No pseudoknot as seen in RESTV.

  
• Roberta Salgado Gonçalves da Silva in Academic Writing

  I have found a website to find conferences around the world, be free to visit http://www.clocate.com/

  I have found a website to find conferences around the world, be free to visit http://www.clocate.com/

  Recent replies ⋅ Show All (1)

  • 
    Sabine Berger replied

    Thanks for the tip. You also have http://lanyrd.com which is great.

    3 hours ago
• Niaz Ali in Genetics

  Why phylogenetic trees constructed with Maximum Likelihood (ML) algorithms are preferred over the neighbouring-joining (NJ) or UPGMA methods?

  I am a beginner, almost no knowledge of the background processing that different packages/software carry out while constructing the phylogeny. I have come across few statements in articles thatI am a beginner, almost no knowledge of the background processing that different packages/software carry out while constructing the phylogeny. I have come across few statements in articles that suggest ML is preferred other methods of phylogenetic reconstruction, but to be honest I did not see any reason why?. I wonder if someone has any suggestions/comments please. Almost 90% of the trees (from 2000-2010) that I come across with have used methods other than ML. Thanks a lot for your time.
• Lifang du in Immunology

  some question about the qRT-PCR

  I want to quantify the DNA copies of HSV2 in cervicovaginal lavage (CVL) specimens and used the gene extracted from the virus as the standard. Ten- fold serial dilutions were prepared with theI want to quantify the DNA copies of HSV2 in cervicovaginal lavage (CVL) specimens and used the gene extracted from the virus as the standard. Ten- fold serial dilutions were prepared with the standard. But when the copies were lower than 1000, the dot would derived from the standard curve. How can I solve the problem?
• Louise Slade in GPCRs

  Question about taxonomy of human bitter taste receptors

  Why are the human bitter taste receptors (hTAS2Rs) located with the frizzled family ? hTAS2Rs share sequence and structural homology with GPCRs of Family A, whereas, there is still some disagreementWhy are the human bitter taste receptors (hTAS2Rs) located with the frizzled family ? hTAS2Rs share sequence and structural homology with GPCRs of Family A, whereas, there is still some disagreement about whether frizzled membrane proteins are actually GPCRs. See http://gpcr.scripps.edu/gpcr_targets.htm
• Guo-Xiang Yang in Molecular Biology

  How to analyze score data by a statistic method?

  we try to evaluate the efficacy of medicine in the protection of hepatitis in animal model. Hepatitis was evaluated by scores according to the severity of disease. what kind statistic method can bewe try to evaluate the efficacy of medicine in the protection of hepatitis in animal model. Hepatitis was evaluated by scores according to the severity of disease. what kind statistic method can be used to compare the treatment and un-treatment group?
• Siddharth Yadav in Organic Chemistry

  Guys help me out on this one!

  Hello Guys! how are yah all! So guys from the O.Chem. Department help me on this one! Guys i am planing a synthesis and here i am stuck! I know how to convert the compound on the right into theHello Guys! how are yah all! So guys from the O.Chem. Department help me on this one! Guys i am planing a synthesis and here i am stuck! I know how to convert the compound on the right into the target but i cant get across this conversion! I want someone to tell me how to get through this step! And pls try to keep it to less steps! because the rest of the total synthesis is already 19 steps long! I even know that there is a bacteria enzyme that does this conversion in one step! but it unfortunately adds a OH to the double bond in the cyclohexene ring! So there mst be some way to do it in 3 or better two or one steps! Thanks guys!

  
• Fereshteh Sedaghat in Cognitive Neuroscience

  Based on your experience regarding attention and memory rehabilitation in MCI patients, what programs, PC software and devices do you recommend?

  Attention and memory rehabilitation strategies and techniques can be of great importance for managing patients with mild cognitive impairment. There are some programs which involve practice ofAttention and memory rehabilitation strategies and techniques can be of great importance for managing patients with mild cognitive impairment. There are some programs which involve practice of cognitive abilities including relaxation, ....and also PC-based programs as attention process training. What is your experience in this regard? Thank you

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