U can generate the current distortion and voltage distortion in terms of faults and u can use the switching of circuit breakers for that

Dear Pavan, Very glad to know you are an oral surgeon now.. Thanks for sharing your experience ... such long term follow up with records will go a long way in changing and improving treatment modalities.

There are a lot of factors that can affect benthic biodiversity along a depth gradient like water temperature, light availability, oxygen (and other elements) concentration and pressure. If you want to observe benthic biodiversity at varying ocean depths, I would suggest that you monitor the distribution and activity patterns of bacteria that are inhabiting the area. Through microbiological and biochemical methods, you can follow the community shifts of bacteria at different ocean depths. This would reveal a lot about the state and condition of that part of the benthos. This is an interesting paper I found that might be relevant to your study: http://www.mbari.org/news/publications/ar/chapters/08_BenthicBiodiversity.pdf And also this study about a long-term observation of benthic activities in central Japan might aid in your own expedition: http://www.oceanobs09.net/proceedings/ac/FCXNL-09a02-1646360-1-KITAZATO%20et%20al%20Ocean%20Obs_withFigs2.pdf

Dear Rohail, A plasmid vectors may be used to clone DNA insert of up to 15 kb in size but all plasmids are not capable of that. only smaller plasmid can take large inserts. Again with the increase in the insert size the efficiency of transformation process decreases. If you are planning to clone large DNA fragment I suggest use differ vectors.

Agree with Guillermo, there is no better predictor than you when it comes to reactions. You just need to take some of the core chemistry classes. JChem might help but might not provide essential information.

Example of frequency bands used in US

Hi there, My suggestion would be to check for intracellular markers. They are more robust in terms of M1 and M2 differentiation. For example, for the classically activated M1 macrophage, you would want to look at iNOS, TNF, and the p38 MAP kinase. On the other hand, the alternately activated M2 macrophage should have high Arginase 1, IL-10, and STAT6. Good luck!!!

Biosurfactants are surface active substances derived from living cells useful in a wide range of industrial application.

agree wid Prof Walid, your question will indeed help us to provide better clarification. Thanks to all contributors.

Alice, could you share any written report/paper/account from that group you know works in Africa?

I think it can be a good idea for Adnan to post your results as a map, so others can either comment on ways of improvement, if needed, or learn more from your case.

In order to use ELISA to detect a protein's presence, you would need to have some purified enzyme already to produce the antibodies required for the assay. In purifying/characterizing the enzyme of interest, SDS-Page would likely be a useful step in determining the degree of purification, molecular weight and/or subunit structure of the enzyme. One reason synthesis of the two EFA's in humans cannot occur because we do not have the enzymes needed to desaturate the C-C bonds at the proper location in the fatty acid chain, so we have to consume these molecules to use them as precursors for making other long chain PUFAs. I would assume this is along the lines of the unidentified enzyme you are looking for. I know that studies have shown some inverts synthesize the EFAs de-novo... so perhaps looking up some of those papers could give you ideas for methods to characterize this metabolic potential in harpacticoids (maybe stable carbon isotopes in acetate, etc.) and the purification schemes used to isolate and characterize the enzymes involved in EFA synthesis (acyl transfer proteins, 'desaturases', things like this). I've never done such work, but I think that a good place to start would be searching a protein / mRNA database for something unique to de-novo EFA synthesis in invertebrates (or maybe even plants/algae/bacteria/yeast; even though they are less likely to be analogous in sequence/structure...they would probably be more prevalent and well studied). A variety of techniques could be used to determine similar molecular characteristics in the copepod. If you found anything interesting/similar/exactly the same then you could attempt to purify and characterize the enzyme (likely involving a whole slew of techniques, not just one), and determine how it is regulated in response to diets differing in their EFA content (protein coding/regulating transcripts, enzyme abundance/location within cells, etc.). These are just a few ideas. Definitely a tedious, time consuming, and difficult process, but something interesting to pursue given the opportunity!

This website might help: http://www.antenna-theory.com/measurements/radpattern.php

I agree with Prof Yuriy, but Baseer sir have you also tried LT PL for slightly higher thicknesses (like 200-300nm), that may give you the rough idea about whether the measurement or the sample is having any issues ?

Thank you Manohar, I read that paper. I maintain the temperature of the bath at 37 to 39 C. But it results tree formation along with nodular deposit. What may be the reason?

Dariusz ,thank you very much.

Plants metabolites (Both primary as well as secondary) that serve as a rich source of bio active chemicals can be evaluated against the target pests (Both pre and post harvest) is an example of Bio prospecting of bio actives. This is a review on "Plant metabolites: an alternative and sustainable approach towards post harvest pest management in pulses" http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3550355/

dear Alberto thank you for helping to me do you think how tools suitable for modeling OTSs? best regard

I think this is happening due to time dependency present in your implementation. Code in debug mode runs slower compared to release mode. Also, different versions of visual studio use different versions of framework and library (generally incremental) and hence you may be getting different results. In Debug mode, lot of debug related information is present in final executable of the program to facilitate debugging (i.e. knowing variable values, seeing stack and automatic variables, step through each line of code and see results etc.). This makes program run slower. In release mode (configuration), the debug information is not present and your code runs straightforward. Debug config is useful in early stages of program development and verification of its correctness. Once it is debugged for errors if any, then release mode executable is produced and shipped (or in technical terms released). Hence debug version of executable is for programmers/developers while release version of code/executable is for the users.

Almost 30 years ago, my teacher taught us how to color slides so that the words would be yellow and the background blue, because he said that research had shown that this was best for the eyes of the viewers. After having watched so many presentation, I still agree with my professor. Your eyes do not get tired so easily when using yellow on blue. So I also ask my students to prepare slides that follow this rule.