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Answer added in Architecture53 Specific tools to evaluate the design of urban public spaces?In doing so the term "Sustainability" should be taken as "mutual sustainability". It must not go towards "Self sustainability" Because firstly it is i... [more]
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Answer added in Rheumatoid Arthritis1 Does the HLA-DRB1 shared epitope bind citrullinated peptides?Dear Jean, I have not studied this but J Hill et al have published this paper which you may be interested in:: Hill JA, Southwood S, Sette A, Je... [more]
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Answer added in Thermal Analysis2 What are the simplest experimental methods that will differentiation between eutectic and co-crystal forming pharmaceutical materials?This is not so easy, because one first needs to be sure that a stable co-crystal has formed. In first instance, mixing two components would lead to eu... [more]
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Answer added in Organic Synthesis83 What are the best reagents to reduce aromatic nitro to amine? Does anyone have suggestions other than Raney Ni, Pd/C, Sn/HCl, Fe/HCl?SnCl2 in 96% EtOH...generous reaction ;-) it always gives over 95% yields...easy and straight forward but use DCM for a work up as SnCl2 is soluble in... [more]
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Question asked in Heavy Metal PollutionNew I am establishing a journal of trace elemnets.......Is any body interested to join us as a member of editorial board.Contact me directly using this e-mail address; moshtaghie@pharm.mui.ac.ir
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Answer added in Plant Tissue Culture3 Osmotic priming or other methods to synchronize germination of radiata pine seeds?Hi, Adrian, I agreed with Frida, GA3 may help, but we used 20-30ppm for rice seed. Good luck!
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Answer added in Fisheries Science3 Is there an example of a subsistence/small-scale fishery that is managed by the fishers themselves, without subsidies, where fish stocks are sustained?Dear Sven, I believe Brazil has such examples, but the hardest point of your question - sustained fish stocks - is almost impossible to get a precise... [more]
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Question asked in Electrical EngineeringNew Can we store the potential generated by the clouds and how to measure it.Lightning strike
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Answer added in Text Mining9 Available tools for text mining, NLP and sentiment analysis.
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Question asked in Surface CharacterizationNew How to measure the surface dipole moment of a solid polymer film?Dipole moment
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Answer added in Semiconductor3 How can i determine the crystal structure nd lattice constants , when XRD fails (very small concentration)?Dear Shivesh Yadav, One possibility to determine the structure of your samples is to inspect them using high-resolution transmission electron microsc... [more]
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Answer added in Cell Signaling3 Hi can anybody suggest me about FRET experiment???Goutam- and do you see the same recovery of signal in both your YFP-tagged gene of interest and an untagged YFP control? FRET is a difficult business,... [more]
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Answer added in Insect Physiology1 Gall insects versus plant chemical defensesHe, have a look at this publication: Plant Resistance Against Gall-forming Insects: The Role of Hypersensitivity Tatiana G. Cornelissen, Daniel Negr... [more]
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Answer added in Environmental Monitoring6 How long can extracted water samples (SPE) in methanol be stored and at what temperature?Wrapped in aluminium foil...
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Answer added in Tumor Immunology8 Optimizing CFSE proliferation assays for T-cells from fresh mouse spleen?Hi Fabienne, I've been meddling with this for a while now. I'll try to answer ur questions in an orderly manner: - how many cells do you label? Whic... [more]
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Answer added in Cell Culture6 Shifting of cells from liquid nitrogen to -70C@ priyanka viabilty will affect a bit less than -120 shows affect if u stores for longer period below to this temp
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Question asked in Text MiningNew Does there exist such spell-checking system that is able to detect not just incorrectly written words, but also syntactic errors?*Boy is playing football. (missing an article) *A boy that five years old. (missing a verb) *A boy give a girl a book upon (extra preposit... [more]
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Answer added in ResearchGate8 Should RG provide a nucleus of expert scientists for specific areas that is available for guidance to a budding scientist?Dear Steingrimur, Nevertheless, asking a question does not assure the reaching of the appropriate audience. The matching of a question via key works p... [more]
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Answer added in Molecular Genetics2 Does the size of the DNA molecule in base pairs correspond exactly to its sequence?Hi Vivian, In general, if it is agarose gel and your fragment is linear, then yes. If it is polyacrylamide gel then it is not always. DNA curvature, ... [more]
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Answer added in Plant Tissue Culture3 Artificial seed productionThe short-medium storage is possible by using encapsulated explants in many species at 4°C. Our experiences were conducted on apple, kiwifruit, lilum... [more]
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Answer added in Fluorescence Spectroscopy10 PMMA film emission spectrum under blue excitation?Nikodem, you can find pure PMMA emission spectrum under excitation at 355 nm in Fig 2b of the paper by Molard et al. in Chemistry - A European Journ... [more]
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Answer added in Protein-Protein Interaction15 Problem detecting the input after a co-immunoprecipitation experiment?Hi Christian, I completely agree with Jason. It is not about winners but interaction with your peers makes you foresee the reactions and concerns that... [more]
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Answer added in Agricultural Science9 Can anyone suggest how we can select heat tolerant plants?I am suggesting to select as many plants as possible and conduct an experiment where you plant that during the time in the year that is hottest in you... [more]
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Answer added in Cyclic Voltammetry12 Is it necessary to use a three-electrode setup to do cyclic voltammetry?Dear Jose, - In general, to use a three-electrode setup is a good idea, as it allows you to decouple current flow from the determination of the pote... [more]
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Question asked in Social ScienceNew Can you recommend literature or do you have any interesting thoughts on theory-induced intervention and/or evaluation studies at PhD-level?I regularly teach PhD-candidates who live and work in a developmentally challenging context so to speak. Their research interests are primarily based ... [more]
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Answer added in Architecture1 Calculation of humidity in construction materialsMight contact the WUFI team and see what they have: http://www.wufi.de/index_e.html
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Answer added in Plant Tissue Culture12 Which is the best surface sterilization proccess?The procedure could change depending on species and season of collection. In general, ethanol sterilizes the surface, but it can dehydrate the tissues... [more]
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Answer added in Bioinformatics and Computational Biology1 Virtual ScreeningSay you have a library of small molecules and an enzyme to target. You want to know which one is the best molecule to inhibit that enzyme. You can scr... [more]
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Question asked in Synthetic Medicinal ChemistryNew what are the differences between CYSTEIN THIOLS found in KINASES and those found in PROTEASES.The mechanisms underlying the action of Proteolytic enzymes are mainly to catalyze the hydrolytic cleavage of peptide bonds while the kinase enzymes c... [more]
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Answer added in Optometry15 Upto what magnitude of Astigmatism do you prescribe without hesitation?Indeed, Very helpful. Thanks.