Questions in Topics
Ask, share and discuss - with a global network of researchers.

Topics makes it easy to ask questions, get answers and share interesting content with other researchers. Browse topics by discipline to find out what's happening in your field.

Browse Topics

A B C D E F G H I J K L M N O P Q R S T U V W X Y Z
  • Question asked in Cancer Biology
    New Is there any way to concentrate the protein after the sample buffer has been added ?
    I need it for Mass spec, western blot. 
    By Arun Pradhan · Cincinnati Children's Hospital Medical Center
  • Answer added in Ethnoecology
    23 Are high-mountain medicinal plants popularly considered more effective than lowland ones?
    By Guillermo Benítez · European University of Madrid
    Saurabh Pandit · Jaypee University of Information Technology
    Hello all. High mountain medicinal plants consist of high medicinal value secondary metabolites. Actually with altitude secondary metabolites content ... [more]
  • Question asked in Primates
    New Any and all Chlorocebus locality data would be greatly appreciated to assist mapping areas of anthropogenic risk to these adaptable monkeys
    I am conducting my PhD research in Primate Conservation at Oxford Brookes University, UK. I am focused on the Chlorocebus genus and the conflict that ... [more]
    By Aoife Healy · Oxford Brookes University
  • Answer added in Plant Tissue Culture
    1 Does somebody have experience with plant in vitro culture on acrylamide gels?
    By Jouve Laurent · L&J Biotech
    Pamela Moon · University of Florida
    another option: Sigma makes a high gel strength agar. It's product number A9799. We use it at 8-9 g/L. 
  • Answer added in Gel Electrophoresis
    2 Is there any way to concentrate the protein after the sample buffer has been added ?
    By Arun Pradhan · Cincinnati Children's Hospital Medical Center
    Arun Pradhan · Cincinnati Children's Hospital Medical Center
    I need it for Mass Spec. 
  • Answer added in Plant Biology
    118 Why are plants green?
    By Ralf Niemann · University of Cambridge
    Buhara Yucesan · Abant İzzet Baysal Üniversitesi
    Now the idea of need energ for seperation of e- from h+ charges is clear to me... :) 
  • Answer added in Fisheries Management
    5 Someone knows a fish population that was administered by more than one stock, no biological justification???
    By Francisco Santa Cruz · University of Concepción
    Allain Barnett · Arizona State University
    Here is one paper written by James Acheson that describes the Maine groundfishery and its shortcomings. Coming up empty: Management Failure of the Ne... [more]
  • Answer added in Teaching English
    8 How do we encourage greater participation in asynchronous discussions?
    By Gary Stobbs · University of Abertay Dundee
    Marie Joubert · University of Nottingham
    In my experience there are a number of things to consider. For example, does the situation feel authentic? Is there a good reason for students to cont... [more]
  • Answer added in Geomorphological Mapping
    3 How do the sampling strategies affect the quality of logistic regression models for landslide susceptibility assessment?
    By Cristian Patriche · Academia Romana
    Filippo Catani · University of Florence
    Dear Cristian, your question is a very central one in landslide susceptibility assessment and it has no definite answer so far. Depending on several ... [more]
  • Answer added in Drug Development
    4 Should serum be added to drug dilutions made in cell culture media?
    By Neha Singhal · International Centre for Genetic Engineering and Biotechnology
    Susan Ross · The Hamner Institutes for Health Sciences
    If your cells are growing in serum, then you are always going to have the issue of drug binding to serum proteins. If you are truly interested in elim... [more]
  • Answer added in Ecology
    5 How can i best economise biological sampling effort on such a large scale, while attaining a sufficient level of richness representation?
    By Ashley Hand · University of Derby
  • Answer added in Ecology
    4 Will the venation pattern of two or more plants of the same species exposed to different light intensities differ?
    By Keth Reston · Ateneo de Manila University
    Saurabh Pandit · Jaypee University of Information Technology
    There are no. Of variations mainly the size, shape and seration of leaves of same species may vary with different exposure to light. But these changes... [more]
  • Answer added in Electrophoresis
    3 What happens when less TBE than mentioned in the recipe was used to prepare gel and run DNA?
    Nadine A. Gund · Bayerische Landesanstalt für Landwirtschaft
    Hi, Spaska is right. Your concentration is higher and the DNA will need more time to run through the agarose matrix. Cheers, Nadine 
  • Answer added in Natural Sciences
    240 Philosophy and Science, what is the connection?
    By Issam Sinjab ·
    Larry Carlson · United Tribes Technical College
    I am familiar with the idea of tradeoffs between energy/matter or wave/particle or position/momentum. An area of contention and confusion seems to de... [more]
  • Answer added in Semiconductor
    1 In chemical mechanical polishing(CMP), what role does the slurry chemical play?
    By Soumendu Sinha · Central Electronics Engineering Research Institute
    Abdelhalim Zekry · Ain Shams University
    I worked with chemical, mechanical and chemical mechanical polishing of silicon. I assume that you have chemical mechanical process as it is the best ... [more]
  • Question asked in Embedded Systems
    New How micro air vehicles are programmed ?
    Which language ? 
    By Venkatakrishnan Muralidaran · SASTRA University
  • Answer added in Delusion
    45 What constitutes a "bizarre delusion" that meets Criteria A for a DSM-IV diagnosis of schizophrenia?
    By Neil Krohn · Sr. Analyst, WellPoint, Inc.,
    Maybe you've all heard about Decarte's Waterloo when it came to the proof of his own belief when his hostess asked if he's like a glass of wine. "I t... [more]
  • Question asked in OPTICAL CHARACTER RECOGNITION
    New Is there any function , or any program to convert binary image to gray scale
    just like we are having a function rgb2gray,im2bw are we having any inbuilt function or any prog for this purpose I need them for determining recogni... [more]
    By Rakesh Singh · Punjabi University, Patiala
  • Question asked in Micro Air Vehicles
    New How micro air vehicles are programmed ? In which language ?
    Do they use a controller or a processor ? 
    By Venkatakrishnan Muralidaran · SASTRA University
  • Answer added in Enzymology
    8 What is the best way of immobilizing an enzyme on a modified glassy carbon electrode?
    By Thabani Mpanza · Mangosuthu University of Technology
    Harikrishnan Jayamohan · University of Utah
    I guess there are umpteen ways to do it. But any pointers on why one is better than other or more importantly what are the factors that determine a "b... [more]
  • Answer added in Academic Writing
    451 Citing Wikipedia?
    By Abdessamad Mouzoune · University Mohammed V Agdal
    Sherry Bryant · Ball State University
    Sometimes, I consult Wikipedia to find out the general idea or definition on a subject. Then, I look for there to be a bibliography to substantiate wh... [more]
  • Answer added in Satellite Image Processing
    15 What is the best software to implement an image processing algorithm?
    By Saeed AL-Mansoori · Emirates Institution for Advanced Science and Technology
    Filippo Catani · University of Florence
    I agree with Jorge suggestion. The coupled use of ENVI and IDL offers the best framework for a remote sensing programmer. Of course, at a cost. If you... [more]
  • Question asked in Nanoparticle Synthesis
    New Synthesis and Detection of Nanoparticles using Dual-Pulse Femtosecond Laser-Induced Breakdown Spectroscopy
    My Dear Friends, I am trying to defense my project "Synthesis and Detection of Nanoparticles using Dual-Pulse Femtosecond Laser-Induced Breakdown Spec... [more]
    By prof. Walid Tawfik · King Saud University
  • Answer added in Plant Biotechnology
    1 Does anybody have whole sequence information of pART7 vector?
    By Hana Zandkarimi · University of Tehran
    Sadeeq ur Rahman · VU University Amsterdam
    http://nanolight.com/Uploads/121%20%20pART27%20info%20PDF.pdf. this is the general map that would help you out for cloning but if you need the whole s... [more]
  • Answer added in Marine Ecology
    12 Is it possible for two Phytoplankton Blooms to occur in the same place, at same time?
    By Sai Elangovan · National Institute of Oceanography
    Florian Koch · Stony Brook University
    A bloom only occurs when growth processes are higher than the removal processes. Growth is influences by bottom up controls such as light and nutrien... [more]
  • 6 What is "passive" and what "active" in electricity and electronics? Can we make passive elements become "less passive", "neutral" or even active?
    By Cyril Mechkov · Technical University of Sofia
    Lutz von Wangenheim · Hochschule Bremen
    Quote Gregg Pulley: "Thus the traditional definition of passive -vs- active, in my thinking should really be based on the whether a device is capable ... [more]