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Question asked in Mammalian Cell TransfectionNew Transfection with human mast cellHi all, What method do you usually use for transfection with human mast cell (HMC-1 cell line)? Does Lipofectamine work well for that cell line? Thank... [more]
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Answer added in Molecular Docking1 I have a base question about analyzing of Docking Score value in docking study for choosing the best pose.Hello. This is always a trick question. For instance, VINA have a standard error of -2.85 kcal/mol, which means that a -12 kcal/mol value have a huge... [more]
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Answer added in Material Characterization16 What are the reasons for peak broadening in the case of nanoparticles compared to the bulk?I read with interest the debate on this issue. However, for me the question is unclear. Author asks to inform about the peak broadening which occurs w... [more]
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Answer added in Statistics12 What is the descriptive power of a model with R-squared equal to 1%?Dear Demetris, Let me reply to all the points mentioned hoping to help further. “1) We cannot escape from the definition of R^2=SS(regr.)/SS(total)... [more]
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Answer added in Nanomaterials9 Could anyone explain how graphene oxide, which is negatively-charged, can become positively-charged when nickel nitrate is added to the GO solution?Although Ni-coordinated graphene oxide is positively-charged, its surface charge is still relatively weak (compared to negatively-charged GO) to preve... [more]
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Answer added in Analytical Chemistry3 we looking for an Indian partnerThank you for your interest, we need just know if you belong for any of those research structures, because our goverment launched a call of project to... [more]
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Answer added in Continuing Medical Education4 What is the importance of journal clubs for junior doctors and medical students?I am a medical student and started a journal club with some colleagues: I find it very helpful to look for the evidence of a therapy or diagnostic pr... [more]
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Answer added in Cell Culture15 Cells remain adherent irrespective of trypsin treatment?Hi Meera, according to the posted site, 10 minutes trypsinization time is the norm. How long it will take depends on how fast your flask warms up. As... [more]
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Question asked in Minimum parameters to evaluate and standardiz the formulatioNew Methods to optimize the the TransfersomesFormulation prepared but the methods to optimize to be followed.
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Question asked in Cognitive ConflictNew Thanks to tue author(s) for sharing this piece. It would be very difficult to find otherwise.We are conducting research on depression using the Repertory Grid Technique. From the time the analysis was done we have advanced new ways of analyzin... [more]
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Answer added in CdTe2 How can i prepare the positive and negative charged surface CdTe quantum dots?thanks fro ur response. Can I prepare CdSe or CdS (positive or negative ) by using same capping?
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Question asked in Management SkillsNew Applied Emotional Intelligence Applications in the Hotel & Leisure Industry - SurveyNowadays Emotional Intelligence is a trending topic, defined as “the ability to notice and to manage emotional cues and information”, therefore I ... [more]
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Answer added in Sustainable Development2 Synergies to increase efficient heat utilizationHello and thank you for your reply, no unfortunately I do not have but I am looking for possibly arena or other engineering/systems simulations to pro... [more]
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Answer added in Material Characterization2 what is the range of Li2O in infrared emission spectrum??To my knowledge, Li20 has no emission in FTIR spectrum range (see : Journal of Physics: Condensed Matter Volume 14 Number 13, 3587 doi:10.1088/0953-8... [more]
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Question asked in Development and evaluation of transdermal productsNew Transdermal delivery of Antiasthmatic agentsTransdermal delivery and evaluation paramters related to the product
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Answer added in Plant Biotechnology32 Sterilization of Arabidopsis seeds?For those seeds sterilization normally try this simple protocol: 1) Put the seeds in water with 2% domestical bleach, during 30 to 40 minutes, keep t... [more]
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Answer added in PCR4 What annealing temp should i use?touch down PCR start with higher temperature (65-67) will work.and 30 -45 second is enough.
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Answer added in Bioremediation4 Why we use acids for heavy metal extraction?Amino acid is highely found in plant, source of growth regulater and many toxic heavy metal found in acidic solution . then we are analysis in HCl ext... [more]
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Answer added in Anaesthesiology7 How frequently do you use awake fibreoptic intubation for predictable difficult intubation?In our center, a reference hospitalary center for pediatrics, in cases with previous failed intubation, fibre-optic is used from the begining.
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Answer added in Analog Electronics17 Why do we prefer using voltage than current as a data carrier in electronics? Why are most of electronic devices implemented as voltage converters?"But if an ideal current source will deliver some energy to a closed circuit-:)?" Provided - such a source does exist in reality. Does it? Cyril, yo... [more]
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Answer added in Medicinal Plants and Herbs7 For the extraction of tagetes erecta,first defatting with pet. ether is essentially necessary?Can Extraction can be done without defatting with ethano...The Lutein esters are soluble in lipophilic solvents. Therefore pet. ether or hexane solve the esters and can not use in a pre defatting process. Fo... [more]
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Question asked in Gel ElectrophoresisNew can anyone give name of free software for determining fragments size of AFLP and RAPD geli want to determine size of all fragments of AFLP and RAPD gel. is there any automated free software which can determine size?
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Answer added in Material Characterization39 What is the difference between XRD pattern of amorphous and crystalline material?Some liquids even show phase transitions from the high density liquid (HDL) to low density liquid phqse (LDL) phase. We have come now very far from o... [more]
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Question asked in Pure MathematicsNew Can we use this dimensional properties for strong projective dimensionStrong dimension concept
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Answer added in Cancer Biology2 Adding FBS and DHS to Growth Media?Dear Rebecca, I would not add FBS to the horse serum. It is only a bottle medium. I would throw it out and start again. Cheers
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Answer added in Bioinformatic Tools3 Which tool will be best for Phylogeny analysis of Protein sequence?MEGA 5 is a good one and also for protein seq
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Answer added in Cancer Biology1 Membrane choice for Western blotting of HIF-1Hi Sean, if anything, PVDF should work better. Did you prewet the membrane with methanol or ethanol before transfer as this is critical? Cheers
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Answer added in Western Blot1 How to stop cytoplasmic proteins from precipitating upon storage?Hi Aparna, perhaps your extraction protocol needs to be modified. Perhaps it is not the pellet that traps the protein but your transfer needs modific... [more]