For the mass analysis of small organic molecules, especially bioactive compounds, which ionization technique is best in Liquid Chromatography Mass Spectrometry (LC-MS)? It is evident that most of the literature is dominated by ESI based LC-MS.

Wanting to construct a schematic of early embryonic development

We need to inventory the valuation methods or analysis methods for sustainability of natural systems. Thus, in this discussion, can you please indicate the methods already developed and indicate a link to related articles or publications?

I'm synthesizing some supported catalysts batches with silica and alumina support. Support particles are in granular shape. The final catalysts should be ground into powder form prior to loading into the reactor. I was wondering if there is a significant difference or any preference between 1) First grinding the granular support, followed by impregnating and drying and 2) Impregnating and drying, followed by grinding.

Before answering this post, make yourself sure you are not a quack, else you shall be deleted. Please check: 't Hooft on bad physicists http://www.staff.science.uu.nl/~hooft101/theoristbad.html John Baez crackpot guide http://math.ucr.edu/home/baez/crackpot.html Siegel's Are you a quack? http://insti.physics.sunysb.edu/~siegel/quack.html

I´m using as secondary antibody: HRP Conjugated anti chicken IgY (http://www.genetex.com/Chicken-IgY-HL-antibody-HRP-GTX26753.html) and TMB substrate (http://www.mpbio.com/product.php?pid=02190277). I´m having problems preparing the substrate. I´m adding 30mg TMB + 5ml DMSO to prepare stock solution. The working solution is prepared as described: 100ul stock solution TMB + Then add 9.9ml of acetate buffer 0.1M and 30ul of H2O2 (30%) for a final concentration of 0.01% H2O2. Please if some one has any suggestions I would really appreciate it. I´m not very experience with this technique.

I have done an EDC Coupling reaction of 3-amino-N-Benzyl pipiperidine with acid chloride. The reaction went neat and clean and the product was isolated. While in H1 NMR I saw a geminal coupling of N- benzylic methylene splitting with 13.2Hz at 3.2 and 3.8 delta scale. I didn't understand how this happened.

One of our students would like to solve antenna placement (or radio network design) problem, using evolutionary computing methods. We have to formulate an optimization problem for her. As I am not expert in antennas, it is difficult for me to point out the most important antenna parameters for such an optimization problem. The formulation of the optimization problem should be as simple as possible (with reduced set of antenna’s parameters), because the focus will be on developing heuristic algorithms. The simplest idea emerging is to place antennas in a set of given sites, so that maximum radio coverage with minimum costs is achieved. In this case the fitness function of an individual will be calculated by radio coverage and costs (for placing or for rental). Would you, please, advise me if such a problem formulation is meaningful?

I'm trying to use the assay described in this protocol to assess bone marrow-derived macrophage migration. Rather than using 35mm dishes, I'm using 6-well plates, which have wells with the same area as a 35mm dish. I find that the medium I've loaded into the wells cut into the gel disappears in an hour or less. I've tried filling the inter-well spaces of the plate with water, but this doesn't prevent the loss. Are my wells drying out or can the medium leak under the agarose? How can I keep this from happening?

I want to count live and dead bacterial cells in the image.

I took 200 sugarcane leaf samples preserved in silica gel abroad for DNA extraction and SSR analysis. After the DNA is extracted and checked in 0.8% agarose gel, the DNA quantity and quality seems good but it failed to amplify using SSR markers. What could be the possible reason?

What are the best alternatives for ethidium bromide in terms of how safe it is to work with? Other factors including price and resolution that it can produce also obviously play a part as well. Any response would be greatly appreciated links would be even better!!

Do you have any insights about it? Case studies of companies, best practices? I am trying to set up a research group on this topic around the following conceptual core: "Milton Friedman consacrated an understanding of the relationship between ethics and business eventually known as 'The Separation Thesis', the main tenet thereof is that business decisions have no moral content, moral decisiones have no business content. This view has been mainstrean in management theory and practice for decades, yet it is being increasingly challenged from many sides. This research line aims at taking stock of recent developments in cognitive sciences, economics, sociology, philosophy, organization studies, and corporate responsibility that point to the manifold ways in which ethics and business are actually embedded. Building on this, it is our purpose to delve into the question, how ethics is driving innovation in every aspects of business, including strategy, organizational design, product, decision making processes, and culture". If you are interested, please contact me at cgcanton@gmail.com

Good day all, I am trying to get the Signal to Interference and Noise Ratio (SINR) and the Signal to Noise Ratio (SNR) at the Physical layer and using a cross layer i will use them at the routing layer. any one who has an idea how i can Compute the SINR and the SNR in NS2.34. I am using the routing protocol AODV. Regards!

I applying IBA and NAA in diffrent concentration (0.3, 0.6, 0.9 , 2 mg/l IBA and 0.3, 0.6, 0.9 NAA and combinatiom of IBA + NAA 0.2, 0.3, 0.4).

An educated populace is the foundation of democracy and is essential to modern economies. Yet student debt is approaching a crisis stage in the US. We not only need to do better, we may need a revolutionary change.

I wish to analyze the lipid content in a solution I created. The surface lipids of lizards were extracted by soaking previously deceased lizard in hexane for 4 hours. I then removed lizards, added ethanol and evaporated hexane from the solution. The resultant mixture was surface lipids with ethanol. I wish to confirm the extraction was successful by measuring lipid content.

Exploration and exploitation of mineral resources from the earth crust and their subsequent conversion of some of the minerals into gaseous form, which are released into the atmosphere, one may conclude that the earth is decreasing in mass. Also, some science and engineering activities may have something to do with the expansion of the earth.

When do you perform a Biopsy in Hepatocellular carcinoma?

I am trying to mutate a residue in my gene of interest that is cloned into a pET28a vector. After transformation, I get no colonies from my LB/Kan plates. I re-designed the primers and repeated the experiment but it still failed. As a control, I tried to transform the template DNA into the XL1 Blue cells and it gave me >100 colonies. This protocol has always worked for me in the past. I was wondering what could be a possible explanation for this.

I need the following temperature dependent properties (varying with temperature) for Nickel (Temperature range of 25-1200 C preferable) 1. thermal conductivity, 2.electrical conductivity, 3.specific heat and 4.expansion coefficient I looked for it in the internet but didn't get them for different temperature.one help? can anyone help?

What different methods of implementing heat-spreaders have been used (wiuth success). I have an application with very small devices mounted to rather large heatsinks, each dissipating over 500W (up to 5 on a heatsink). I would like to come up with a more effective heat trasfer from the device to the cooling air. Heatpipes, and copper spreaders are but a few examples - I'm looking for more and different ideas.

Apparently there are three main categories: near-field acoustic holography, acoustic beamforming, and inverse methods. Are there specific criterias on how to select one from another?

For non-native speakers it can be easier to write in their mother tongue. Moreover, pride in ones language can play a role. However, the language of science is English and writing science in other languages has, therefore, drawbacks. I wonder how this issue is navigated internationally.

Will expression of the interested protein, behave similar in both stable and transient transfection protocols?

I used Lyso Tracker Red to characterize acidic organelles or structures within young maize endosperm cells. Surprisingly, the dye stains not only small structures like autophagosomes, it also labelled huge ring-like membrane structures of about ~ 15 µm in diameter.

I am making a coupled temperature-electrical-structural model for spark plasma sintering. I have used Abaqus standard. The model worked for simple geometry involving only 3 parts (2 punch and die), but it is not converging for my original model which has 2 punch, sample, die, 4 spacers. The error that I am getting is - Too many attempts made for this increment There are few warning messages aswell 1. Displacement increment for contact is too big 2. SURFACE INTERACTION "interaction name", ASSOCIATED WITH ONE OR MORE CONTACT PAIRS WITH SURFACES HAVING UNDERLYING ELEMENTS WITH ELECTRICAL, TEMPERATURE, AND DISPLACEMENT DEGREES OF FREEDOM, DOES NOT HAVE ANY ELECTRICAL INTERACTION DEFINED. NO ELECTRICAL INTERACTION WILL OCCUR. I used to SI units and applied a pressure of 5 MPa at the top of upper spacer. Can anyone help me? I have attached the input file here

I specifically want to find/be informed of a model/technology that can minimize waste pollution in surrounding rivers Dyeing is the process of adding colour to textile products like fibres, yarns, and fabrics. Dyeing is normally done in a special solution containing dyes and particular chemical material. After dyeing, dye molecules have uncut chemical bond with fiber molecules. The temperature and time controlling are two key factors in dyeing. source:wikipedia