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  • Answer added in Endodontics
    55 What do you think about the lack of agreement concerning irrigation?
    By Wojciech Wilkoński · Collegium Medicum of the Jagiellonian University
    Yango Pohl · Rheinische Friedrich-Wilhelms-Universität Bonn
    Yes, maybe, I admit, I did not get deep into the issue. But, again, can you tell - in some sentences - how to do it practicallly? Concerning NaOCl. a... [more]
  • 1 Patch clamping cultured neurons
    By Gyorgy Lur · Yale University
    Miroslav Nenov · University of Texas Medical Branch at Galveston
    Hi! How about to try some HEPES-based saline solution with adjusted pH instead of using aCSF or neurobasal media which ususlly require oxigenation. ... [more]
  • Answer added in DNA Cleavage
    5 Is it possible to study the DNA cleavage activity of a water -insoluble complex?
    By Sujatha Arunkumar · University of Madras
    Kalyan Mukherjea · Jadavpur University
    you are welcome, Sujatha 
  • Answer added in Organic Semiconductors
    7 Why is the turn-on-voltage of OLED high and furthermore, the devices getting burned?
    By Sanjib Das · University of Tennessee
    Yongbiao Zhao · National University of Singapore
    Have you done O2 Plasma treatment of ITO? What is the thickness of each layer? The normal structure of this device should be ITO (O2 treated)/NPB(70 t... [more]
  • Question asked in Cell Line
    New Is SK-MEL-28 melanotic or amelanotic? I am getting publications showing both the ways?
    You have shown it as a melanotic cell line and even I believe so but there are some papers that show it the other way. Can you please give an explanat... [more]
    Psoralen activity and binding sites in melanotic and amelanotic human melanoma cells. M C Isoldi, A C Scarparo, R I Schumacher, A M Castrucci
    By Neel Fofaria · Texas Tech University Health Sciences Center
  • Question asked in filamentation
    New How does the nonlinear absorptive effects influence energy exchange between two filament-forming laser pulses?
    When the intense fs laser pulse is focused in air, it will generate a thin filament. If two filament-forming pulses spatial overlap and couple, one ca... [more]
    By P.J. Ding · Lanzhou University
  • Answer added in Plasmonics
    10 Can anyone suggest a Profilometer for measuring film thickness with nanometer resolution?
    By Venkatesh Sri · Sri Sathya Sai Institute of Higher Learning
    Adithi Umamaheswara · Mount Carmel College
    It is almost like a ellipsometer, where you don't need any step height. It can also give the refractive index. In ellipsometer you can get the thickne... [more]
  • Answer added in Gel Electrophoresis
    1 What is difference between TAE and TBE buffers and their properties regarding use in agarose gel electrophoresis?
    By Niraj Tripathi · Jawaharlal Nehru Krishi Vishwavidyalaya
    Upendar Golla · Indian Institute of Science Education and Research Bhopal
    TAE (Tris-acetate-EDTA) buffer is used as both a running buffer and in agarose gel. Its use in denaturing gradient gel electrophoresis methods for bro... [more]
  • Answer added in Clinical Pharmacology
    9 What is the best software for statistical analysis of pharmacokinetic parameters? Please suggest.
    By Jahnavi Srividya · Acharya Nagarjuna University
    Nilesh Meghani · Ajou University
    i would recommend winnonlin too as it is easy to handle and gives good enough data.... 
  • Question asked in Advertising
    New Can advertising create "want" in the market?
    Needs are innate , they can never be created. Needs lie dormant, marketers can only bring needs to the surface. Wants are more specific and can be inf... [more]
    By Soma Sinha Roy · IIT Kharagpur
  • Question asked in Open Access Publishing
    New What is the contribution of journal indexes to research quality?
    Questioning the foundations of journal indexation 
    By Rafael Hernandez Barros · Complutense University of Madrid
  • Answer added in CERN
    4 I wrote a paper named "Thermal Neutron Accelerator" which can accelerate neutrons ! I need to get my paper reviewed .... Please Help Me ???
    By C.Amar Prasad · Andhra University
    C.AmarEshwar Prasad · Andhra University
    Mr. Tarun Verma, and Mr. Bilal Shafique Sir actually my paper "ThermalNeutron Accelerator " accepted and received call from: i) (World Academy of S... [more]
  • Question asked in Microbial Molecular Biology
    New Are gram-negative microbes better communicators than gram-positive ones vis a vis the paper here?
    Periplasm may be an advantage to gram-negative microbes over gram-positive ones. 
    By Rakesh Yashroy · Indian Veterinary Research Institute
  • Answer added in Postharvest Handling
    2 I have a question regarding the measurement of the concentration of bacteria in the vase solution have?
    By Fatemeh Hashemi · University of Maragheh
    Fatemeh Hashemi · University of Maragheh
    Hi, You have a method for measurement of the concentration of bacteria in the vase solution? Thanks 
  • Question asked in Cell Culture
    New Please tell me any analogue for guanine?
    Analogue for guanine that can be used in cell culture 
    By Anushree Gupta · Bangalore University
  • Question asked in Nanoparticle Synthesis
    New VEGF PLGA nanoparticles
    I am currently working with VEGF PLGA nanoparticles for lymphedema treatment. I am using PVA 1% as a stabilizer and dissolving 0.1gm PLGA in 4ml DCM. ... [more]
    By Kshitiz Shrestha · Catholic University of Korea
  • Answer added in Cell Culture
    7 Does anybody know what is the safe solution of DMSO for cell cultures?
    By Carlos Moro · Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria
    ChandraSekhar Misra · Manipal University
    I am agree with Sundarajan Kannan and Angie Dull. I have done experiments on RAW cells. To increase the solubility I have used 1% of DMSO. But when I... [more]
  • 2 A patient who is classified as "heterozygous" could have a mutation that has not yet been identified. Can false heterozygous cases be ruled out?
    By Janine Jagger · University of Virginia
    Janine Jagger · University of Virginia
    Thank you for your informed response. Would sequencing of the whole exome stand to yield anything more than sequencing of the MEFV gene? Are you look... [more]
  • Answer added in Ecology
    3 Measuring the sustainability of an ecosystem by the relationship of species.
    By Konstantin Kitaev · Ufa Scientific Center of the Russian Academy of Science
    Konstantin Kitaev · Ufa Scientific Center of the Russian Academy of Science
    I know diferential equations but this isn't I need. Relationship of species isn't only predator-prey relationships. And my model isn't deterministic,... [more]
  • Answer added in Crystallography
    3 Where can I find the crystal structure of 2-Formylbenzonitrile, 3-Formylbenzonitrile, and 4-Formylbenzonitrile?
    By Shaumik Ray · Council of Scientific and Industrial Research (CSIR), New Delhi
    Vaibhav Kaware · University of Pune
    Check Crystallographic Open Database. I too am searching for 3 and 4 Formylbenzonitrile cell parameters. 
  • Answer added in Security Studies
    11 What role has Iran played in the current war in Syria and can its influence withstand the fall of the current regime?
    By Chistine Roberts · Deakin University
    Chistine Roberts · Deakin University
    Agreed. It must also be remember that Rowhani lead the nuclear negotiations and has started he believes Iran should have a nuclear capacity although h... [more]
  • Answer added in Glycobiology
    7 Is there any bioinformatic tool(s) to predict protein-carbohydrate (glycan) interaction?
    By David Simon Davis · Australian National University
    Nandakumar Yellapu · Sri Venkateswara University
    Exapsy prosite tool can predict all post translational modification sites in a single step. just need to scan the protein sequence, but not sure how f... [more]
  • Answer added in GPU programming
    6 GPU: floating-point double precision vs. 64-bits fixed-point? What will be the difference in execution time on an Nvidia TESLA?
    By Mario Casu · Politecnico di Torino
    Lubomir Antonov · University of Copenhagen
    Robert, gaming GPUs have exactly the same precision as "non-gaming" GPUs. The difference is in using ECC memory (and more memory), and in the case of ... [more]
  • Question asked in multi-depot vehicle routing problem
    New Please, I want a version of this paper.
    Please, I want a version of this paper. 
    By Abdelmonaem Abdallah · University of New South Wales
  • Answer added in Oxidation
    2 Can i get the details of aldol reaction?
    By Sankar Hari · Don Bosco College
    Krishnasarma Pathy · NET MATRIX INDIA LTD
    Carbon-Carbon bond forming reaction and the basic mechanism of the aldol condensation reaction.A useful carbon-carbon bond-forming reaction is known a... [more]
  • Answer added in Engineering
    10 Why 'Good Teaching' is not rewarded?
    By Girish Kumar Ramaiah · Don Bosco Technical Institute
    Manasi Patil · SVERI´s College of Engineering
    In my opinion research and teaching should go hand in hand and balanced. Whenever this balance is not there problems will be created. Without researc... [more]
  • Answer added in Wave Propagation
    3 What kind of interaction is possible between a sound wave and an EM wave?
    By Srinivasa Raju · Indian Institute of Space Science and Technology
    Srinivasa Raju · Indian Institute of Space Science and Technology
    Thank you Mircea Pop & Patrick Bisson 
  • Answer added in PCR
    5 Could histones interfere in a PCR process?
    By Norberto Santillán Padilla · National Autonomous University of Mexico
    Deepak Modi · Indian Council of Medical Research
    We routinely do PCR of crude samples (mouse tails often). Except for a quick protease treatment b4, everything is petty much there (no purification cl... [more]