Questions in Topics
Ask, share and discuss - with a global network of researchers.

Topics makes it easy to ask questions, get answers and share interesting content with other researchers. Browse topics by discipline to find out what's happening in your field.

Browse Topics

A B C D E F G H I J K L M N O P Q R S T U V W X Y Z
  • Question asked in Cervical Cancer
    New Can any one send me this paper please? Expression and humoral response of a-kinase anchor protein 4 in cervical cancer.
    AKAP4 in cervical cancer 
    By Nirmala Jagadish · National Institute of Immunology
  • Answer added in Online Learning
    2 How can we best measure the effectiveness of our instructional material?
    By James Baker · Carnegie Mellon University
  • Answer added in Theoretical Chemistry
    10 What will be the problem in a Geometry Optimization calculation if we are not given Grid3 option in the input file?
    By Priyabrata Banerjee · Central Mechanical Engineering Research Institute
    Susanne Klein · Max Planck Institute for Chemical Energy Conversion
    The input file you uploaded still has an %scf block. So maybe I misunderstood your post. Would you please upload the output file? It would it make eas... [more]
  • Answer added in Microbiology Techniques
    2 Can anyone please tell me the institution offering SEM service for bacterial sample in India?
    By Ali Asger Bhojiya · Mohan Lal Sukhadia University
    Ali Asger Bhojiya · Mohan Lal Sukhadia University
    thanks for reply. I contacted AIIMS and IIT-Delhi for SEM service and they both replied that there SEM/TEM systems will not be functional for 3-4 mont... [more]
  • Answer added in Entomology
    17 How can I prevent insect hemolymph from coagulation?
    By Basma Gabr · Damietta University
    Panna Ali · Bangladesh Rice Research Institute
    Hi How you collected, I want to know? Now-a-days frequently I am collecting hemolymph but no coagulate. Please more clarify ur methods and purpose. 
  • Answer added in Medical Research
    4 Is there any pubmed indexed journal which accepts videos along with medical case reports?
    By Gyanshankar Mishra · Government Medical College, Nagpur
    Esther de Ru · GoPhysio & Centro Mar y Salud
    The BioMedcentral journals are all open access. Go to http://www.biomedcentral.com/journals for the full long list of the many journals involved. 
  • Answer added in Organic Semiconductors
    10 Why is the turn-on-voltage of OLED high and furthermore, the devices getting burned?
    By Sanjib Das · University of Tennessee
    Narayanan Unni · Indian Institute of Technology Kanpur
    Since you don't do oxygen plasma treatment, your ITO workfunction will be somewhere near 4.6-4.8. NPB has a LUMO of 5.2 eV. That means the offset can ... [more]
  • 7 Gaseous Flux Measurements
    By Manthan Tailor · The Maharaja Sayajirao University of Baroda
    Simone Mereu · Università degli Studi di Sassari
    Wow! This is an interesting problem. I also never worked with landfill and I'm not an expert on Methane emissions (although they are also very interes... [more]
  • Answer added in P Value
    75 What is the use of reporting the exact p value in a research paper? Is it necessary?
    By Brijesh Sathian · Manipal College of Medical Sciences
    Jochen Wilhelm · Justus-Liebig-Universität Gießen
    No, Eik, I think you did not get me (or did I make a typo using the wrong sign somewhere?). P(p>0.9|H0) = 0.1, that's right, but this would be a test ... [more]
  • Answer added in Plant Ecology
    10 How to remove wax from the leaves of Populus to make stomatal imprints?
    By Anna Jasińska · Polish Academy of Sciences
    Mirko Granata · University of Pavia
    Dear Anna, the real problem in Populus tremuloides, Populus alba is not the wax but the problem is caused by the hair. If you use some simple adhesive... [more]
  • Answer added in Quantum Mechanics
    11 Why in the ground state of any QM system (s state) is the angular momentum always zero...?
    By Abdul Kaliyar · Indian Institute of Technology Madras
    Tuhin Mukherjee · The Institute of Mathematical Sciences
    It's the expectation value of the angular momentum in the ground state which is zero. 
  • Answer added in Tropical Diseases
    9 How has the Dengue epidemic in your area been this year?
    By Indrajit Suresh · JSS Medical College, Mysore
    Rafael Toledo · University of Valencia
    increase the BSA or milk concentration in the blocking buffer 
  • Answer added in Conservation Biology
    26 Looking for a reliable website with pictures and botanical identification of plants
    By Rita Lemos · Universidade Federal de Ouro Preto
    Anna Jasińska · Polish Academy of Sciences
    Http://www.atlas-roslin.pl/ 
  • Answer added in Theoretical Physics
    57 Can singular points be actual?
    By Juan-Esteban Palomar Tarancon · I am retired
    Dear Professor Juan-Esteban In applied mathematics the term singularity is used to indicate that a conventional way of modelling a certain physical ... [more]
  • Answer added in Computer Aided Drug Design
    1 Who could explain the steps of biological evaluation of new synthesized molecules?
    By Amac Tuyun · Beykent Üniversitesi
    Taibi Ben Hadda · Université Mohammed Premier
    Send me your e-mail, I have some thing for you. http://www.youtube.com/watch?v=lFo3hZpVut8 http://www.youtube.com/edit?video_id=lFo3hZpVut8&video_ref... [more]
  • Answer added in Bacterial Cellulose
    5 Dispersion of nanofiberous cellulose
    By Fatemeh Ostadhossein · Sharif University of Technology
    Fatemeh Ostadhossein · Sharif University of Technology
    Thanks for all answers with regard to Mr. Heidarian, I wanted to add that I have followed the same procedure (stirring+subsequent ultrasonication) but... [more]
  • Answer added in Protein-Protein Interaction
    3 Can I use Co-IP to evaluate interaction over time?
    By Consuelo Olivares · Pontifical Catholic University of Chile
  • Question asked in Gene Regulation
    New How to get the entrez id from gene symbol?
    Gene symbol is known but how to find entrez id 
    By Anupama Tripathi · Vellore Institute of Technology
  • Answer added in Rumen Microbiology
    14 Running a genomic DNA quality check on a gel - can anyone help?
    By Lindsey Angharad · University of Queensland
    Felix Eikmeyer · Universität Bielefeld
    Hi Lindsey, I am a bit confused because your positive control indicates a fragment size of about 200-300 bp, so what does this control contain? Sever... [more]
  • Answer added in Cellular Biology
    10 Which is the best reference protein for Western blotting on plasma membrane?
    By Fatima Perez de Heredia · Liverpool John Moores University
    Luisa F. Jimenez · Max von Pettenkofer-Institut
    If you can prepare your samples in non-reducing conditions (meaning without Mercaptoethanol or DTT in your sample buffer) you can use the detection of... [more]
  • Answer added in Information Technology
    1 What is the difference between data & information??
    By Nyeinchann Aung · Albukhary International University
    Marc Walterbusch · Universität Osnabrück
    Data: Combined characters for the purpose of processing Information: Action-relevant knowledge More input can be found here: http://www.diffen.com/di... [more]
  • Answer added in Programming Languages
    632 Which first language do you think is the best for learning programming techniques?
    By Carlos Bazilio · Universidade Federal Fluminense
    Wojciech Borkowski · University of Warsaw
    I teach mostly psychologist with almost no computer science background. Many years I used simplified Pascal with easy graphics (called ALGO) . Now it ... [more]
  • Answer added in Biomarkers
    11 What are good cellular markers of aging? How can I accelerate aging in cells?
    By Alexander Henke · Universität Köln
    Alexander Henke · Universität Köln
    Hello Andrei, all valid criticism. A few clarifications, just to avoid misunderstandings: HEK293 is an adherent cell line but with low attachment to... [more]
  • Question asked in Animal Models
    New Which are the most precise methods of measuring dopamine levels in different parts of the rat brain?
    I was thinking about PET scan of dopamine system, but I think dopamine tracers are not so easily available to order. 
    By Jelena Suran · University of Zagreb
  • Answer added in Radio Telemetry
    2 I have radiotagged data on 9 individuals. In order to find the home-range, should I use them separately or put them together and find just 1 homerange?
    By Alessandro Ferrarini · Università degli studi di Parma
    Alessandro Ferrarini · Università degli studi di Parma
    Hi Vladimir, thank you The species is Lesser Kestrel Falco naumanni. I'm interested in particular in habitat preference, I've done analysis using Neu,... [more]
  • Answer added in Sleep
    18 A good biomarker for sleep quality measurement?
    By Bruno Giacobbo · Pontifícia Universidade Católica do Rio Grande do Sul
    Wessel M.A. van Leeuwen · Stockholm University
    I would highly recommend to use the Karolinska Sleep Diary to determine sleep quality. See the attached paper. 
  • Question asked in Social Services
    New What curriculum is most effective in training direct care staff on how to work with traumatized youth in a group home setting?
    I run a short term assessment program for children and youth who have frequently experienced various forms of trauma and many present with challenging... [more]
    By Coll Felton · Springfield College
  • Answer added in Genomics
    3 facing problem about the cDNA purity...Rna extracted by LiCl method
    By Ravinder Mehra · Chaudhary Devi Lal University
    Estanis Navarro · IDIBELL Bellvitge Biomedical Research Institute
    As Martin said, the critical point is the downstream application. I've never purified a cDNA, and for standard applications (PCR, qPCR,....) I don't t... [more]