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  • Answer added in Delusion
    35 What constitutes a "bizarre delusion" that meets Criteria A for a DSM-IV diagnosis of schizophrenia?
    By Neil Krohn · Sr. Analyst, WellPoint, Inc.,
    Dale Pietrzak · University of South Dakota
    It becomes grey quickly really - as I see it. As it is really the persons cultural/social group that is of import here. There may be very few of a su... [more]
  • Answer added in Western Blot
    7 What is the best loading control for membrane fraction western blot?
    By Susitha Premarathne · Griffith University
  • 35 Imidazol concentration for His-Trap purification
    By Anette Martensson · Lund University
    Gary Laco · The Roskamp Institute
    I agree with Mark about your low binding problem, if the imidazole concentration was too high your protein would not bind at all. It could be that you... [more]
  • Answer added in Distribution
    1 What can I do with severely skewed distribution before applying a factor analysis or Alpha reliability?
    By Mona Ahmed Hassan · King Abdulaziz University
    Alessandro Giuliani · Istituto Superiore di Sanità
    Factor Analysis is a descriptive technique so why bother about distributions ? The only possible problem could arise by the sensitivity of correlation... [more]
  • Answer added in Animal Nutrition
    4 Does anyone know if guava is harmful to some primates like lemurs ?
    By Yesenia Escorcia · Fundación Zoológico de Barranquilla
    Stacey Tecot · The University of Arizona
    Eulemur rubriventer also eat Chinese guava in Ranomafana National Park (Overdorff 1991; Tecot 2008). 
  • Answer added in Organic Synthesis
    3 What is the best condition for Diels-Alder reactions using Acrolien as dienophile?
    By Mujeeb Sultan · King Saud University
    Marcin Budny · Nicolaus Copernicus University
    Lithium perchlorate in diethyl ether is also popular. 
  • Answer added in Western Blot
    12 How to separate lipid contaminants during protein extraction of animal tissues?
    By Sureshbabu Angara · Yale University
    Sophie Cazanave · Virginia Commonwealth University
    a little expensive but absolutely amazing to get rid of this fatty layer is to use the Columns from the RNaesy minelute cleanup kit from qiagen. You j... [more]
  • Answer added in Membrane Proteins
    1 Acrylonitrile ultrafiltration membranes
    By Somayeh Alibakhshi · Isfahan University of Technology
    Espinoza-Gomez Heriberto · Autonomous University of Baja California
    In most cases, to make ultrafiltration membranes, acrylonitrile copolymers are used (maleic acid, 1-methyl-pyrrolidone, polysulfone, acrylamides, e tc... [more]
  • Answer added in PCR
    2 How long can I keep the prepared master mix before running the real time pcr reaction?
    By Sahar Tahvili · Lund University
    Issaka Maman · Institut National d'Hygiène, Lomé-Togo
    I agree with Maria. I think if you meet a problem just after mix everything even RNA or DNA, it's also possible to store your preparation about one to... [more]
  • Answer added in Diabetes
    65 Benefits of the "Paleo" diet for health and performance. Are there any? And is it relevant to consider what we evolved to eat?
    By David Hamilton · University of Stirling
    Alexander Schauss · AIMBR Life Sciences Inc.
    Sydney, you raised a very important point about the "vitamin E" studies that reported a slight increased risk when taken as a dietary supplement by to... [more]
  • Answer added in Gel Electrophoresis
    3 Alternatives to Trans-Blot Turbo PVDF packs
    By David Place · Pennsylvania State University
    Aman Makaju · University of Utah
    I use my own gel, and my own blotting stack and i have had no problems so far. The transfer seems comparable. I usually use the 7 min mixed MW transfe... [more]
  • 4 White precipitates in eluted histagged purified protein?
    By Nur Faezee Ismail · University of Science, Malaysia
    David Bateman · National Institutes of Health
    Sounds like your protein is aggregating. You should know the concentration after purification, use OD or Bradford. Just check now to see what the OD... [more]
  • Answer added in Amyloid
    2 Unexpected response to carbonic anhydrase in IAPP Fibrillation?
    By Jetaime Ross · University of Maryland, Baltimore
    Jetaime Ross · University of Maryland, Baltimore
    Wow, thank you for bringing that to my attention! I believe carbonic anhydrase naturally contains zinc. I'm now wondering how others who worked on my ... [more]
  • Question asked in Targeted Drug Delivery
    New What drug delivery systems are promising for cancer, particularly HCC?
    What are the advantages and problems with using bacterial magnetosomes? How about for iron nanoparticles? My main concern stems from 1) the biodegrada... [more]
    By Luke Lazatin · Ateneo de Manila University
  • Answer added in Evapotranspiration
    5 Is there any website offering historical meteorological data?
    By Noha Abdulwarth · Agricultural Engineering Research Institute
    Mohamed Mattar · King Saud University
    Hi Noha, you can use this website: http://eosweb.larc.nasa.gov/cgi-bin/sse/interann.cgi 
  • Answer added in Isotope Geochemistry
    12 I need to date two samples (Ar/Ar methods) for my PhD proposal. Can anyone help by doing this for me? Iran does not have geochronology facilities.
    By Majid Ghasemi · Kharazmi University
    Martin Menzies · Royal Holloway, University of London
    Majid is a PhD student. I presumed like many PhD students he has little time and/or funding, so Rb-Sr could work out easier, cheaper and faster than A... [more]
  • Answer added in Hedgehogs
    5 Does litter size provide a reliable indication of the health of a species?
    By Toni Bunnell · The University of Hull
    That is great. Sound like you have an unique opportunity to examine several key factors together, which has not been possible for many other situatio... [more]
  • Question asked in Genetic Association
    New I can't get clear bands on agarose gel from wsp region, Does anyone know how they can be sharp or clear?
    I can't get clear bands on agarose gel from wsp region, Does anyone know how they can be sharp or clear? 
    By Sara Rohollahi · University of Tehran
  • Answer added in Spatial Analysis
    6 Which is the recommended method for spatial mapping of soil characteristics as pH texture?
    By Sachin Patil · Indian Institute of Technology Bombay
    Jorge Delgado · Universidad de Jaén
    I recommend you the use of geostatistical tools -estimation like kriging or simulation-. For this type of applications (ph mapping), I prefer conditi... [more]
  • Answer added in Statistical Analysis
    7 Is inappropriate sampling (e.g., non-random assignment) considered to be major weakness of a submitted manuscript to be accepted?
    By Fathi M Sherif · University of Tripoli
    John Kern · Kern Statistical Services, Inc., University of Wyoming, Montana State University
    I don't think justifying "why" the sampling was non-random is enough unless it is also possible to quantify and correct the bias that is transferred ... [more]
  • Answer added in Cancer Biology
    12 Can you suggest a good review on ion channels and cancer?
    By Elena Adinolfi · Universita degli studi di Ferrara
  • Answer added in Formal Verification
    5 How to select the best Verification Tools for Observational Transition Systems (OTSs)?
    By Alireza Souri · Islamic Azad University
    Alberto Lluch-Lafuente · IMT Institute for advanced studies
    I agree Pierre and Dorel. As for Maude I would suggest the Primer (http://maude.cs.uiuc.edu/primer/) as a first introductory text/tutorial. Then you c... [more]
  • 20 Should we choose open-source GIS softwares or the commercial ones?
    By Sokunthet Sok · Hochschule für Technik Stuttgart
    Jorge Delgado · Universidad de Jaén
    A couple of additional options of free GIS software Kosmo: http://www.opengis.es/ gvSIG: http://www.gvsig.com/welcome?set_language=en I hope that the... [more]
  • Answer added in Drug Analysis
    6 How can you avoid problems in a GC column due to an excess of a derivatizing agent?
    By Luis Bartolomé · Universidad del País Vasco / Euskal Herriko Unibertsitatea
    Which compound are you derivatizing? Do you need the derivatization step? 
  • 4 Why is p-type ZnO not stable?
    By Gopinath Danda · KIIT University
    Yuriy Gnatenko · National Academy of Sciences of Ukraine
    P-type ZnO can be obtained by Sb doping. This information is in next paper. 
  • Answer added in Monitoring
    7 Are you still using CVP for fluid management, do you find it useful most of the time?
    By Ghazi Aldehayat · King Hussein Medical Center
    Souvik Maitra · All India Institute of Medical Sciences
    Most of the time CVP is useless, it is particularly evident when you compare CVP with SVV in a particular clinical scenario. 
  • Question asked in Education
    New Does anyone know of research undertaken in the filed of visual learning and second language development?
    Does anyone know of research undertaken in the filed of visual learning and second language development? 
    By Audrey Beaumont · Liverpool Hope University
  • Question asked in Evidence Based Medicine
    New Can someone tell me how it is globally sought to make people sensitive to problem-oriented knowledge about health and disease
    Do we really only need GOOGLE knowledge, or transformation of science into all areas of life? 
    By Heinz Spranger · Interuniversitäres Kolleg für Gesundheit und Entwicklung