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Answer added in Natural Resource Management10 Is anyone aware of examples from around the world where subsistence fishers are investing financially in ongoing fisheries management?For examples from South Africa, you could look up the work of Samantha Williams. I am not in fisheries, but in sustainable harvesting of plant species... [more]
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Answer added in Research Ethics5 Are there any types of human experiments for which ethics committee approval is generally not needed?Hm, to do research on your fellow staff might easily evoke ethical concerns, for example if a senior staff member asks juniors to take part, then juni... [more]
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Answer added in Elemental Analysis1 During elemental analysis of an organic material are the total solids destroyed or is there some residual ash content left?Elemental analysis for C, N, S, H, H,and O. The mechanism is like reactor. The combustion is occurred during analysis first sample goes to furnass wh... [more]
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Question asked in Organic SynthesisNew Is it possible to introduce aldehyde group by Vilsmier reagent on fused nitrogen heterocylcles or benzazepines?Has anybody tried?I want to avoid oxidation of alcohol to aldehyde
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Answer added in Conservation Biology11 Where should we publish or how many publications should we have? Which is better for young ecologists?A major determinant of accepting ecological research by prominent journals in ecology is the experimental design, how current the methodology is and t... [more]
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Answer added in 1H-NMR19 How to avoid a broad signal in H-NMR spectra due to self-aggregation between analyte molecules?I agree with Conner. When running a chemistry NMR facility, the most common cause of broad lines/aggregation I saw was high sample concentration. If y... [more]
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Answer added in XRD Analysis6 SAED patternIf your sample is "large" enough you could use SEM to obtain Kikuchi pattern. Something like: http://www.ebsd.info/A52.pdf
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Answer added in Evolution4 Is complete lineage separation possible in the process of sympatric speciation?Dinesh, can you please elaborate.
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Answer added in Food Chemistry3 What edible chemical could be used to increase the gelling of Custard?I would try methyl cellulose; it thickens the custard cold, interacts with the starch to strengthen its gellation and if the custard is heated, it the... [more]
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Answer added in Mammalian Cell Transfection2 Have you ever tried serum starvation right after siRNA transfection?If you are doing 2 transfections is it because you are dealing with a long lived protein, which is harder to knock down? If so I believe before you d... [more]
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Question asked in Molecular Biological TechniquesNew Problems with Transformation / DH5-alpha / Plasmid DNA ??!!We have cloned a gene in vector pVITRO2 (GFP-HYG-LacZ; 10KB), and transformed it into DH5-alpha E.coli cells with suitable antibiotic (of course in th... [more]
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Answer added in Conservation Biology18 How to measure spatial isolation throughout the distribution?Getis-Ord General G (Spatial Statistics) is a High/Low Clustering tool which measures concentrations of high or low values for an entire study area. A... [more]
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Answer added in 5' RACE5 Mammalian genes with very short 5' untranslated region?Peter, Generally, 5' UTR is highly correlated with translational regulation, especially for the regulation on initiation. Thus, I think the variatio... [more]
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Answer added in Apoptosis Assays2 Does anyone know a protocol to demonstrate phagocytosis of apoptotic sperm in testes of insects?We produce magnetic particles to deplete apoptotic sperm. I would suggest the capture of apoptotic semen then subjecting them to 50 nm or less magneti... [more]
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Answer added in Cell Culture151 What are the best kits for RNA isolation?Qiagen is the best
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Answer added in Image Processing11 What is the best method for edge detection that gives the biggest amount of data, code available?you can find many in Matlab Image Processing ToolBox ,, like edge(), del2(),LoG, canny, sobel, prewitte operators Morphological operators can also gi... [more]
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Question asked in NeurobiologyNew Serotonin receptors antibody for fishDear all I am currently trying to preform some immunohistochemistry studies in goldfish and I need to find anti-bodies for the HTR1F, HTR1E, HTR3a an... [more]
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Answer added in Algorithm Development4 Finding whether an array is sorted or not@Joachim. For the problem precisely described in the question, try to do better!
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Question asked in Basal GangliaNew Seeking to team-up for project in dopaminergic and serotonergic signaling in choice behaviourI am PhD student available for 3 - 16 months research project in neuroimaging out of Italy. I would be grateful for any research contacts/projects in ... [more]
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Answer added in PCR6 qPCR (absolute quantification using the standard curve method)Just one comment on Elia's note: While the use of an absolute standard curve is accepted as the most reliably form of qPCR quantification, I need to c... [more]
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Answer added in Education1 Is co-educational or single sex education best for primary school children?In my opinion co-ed school is better at all levels of education.
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Answer added in Immunohistochemical Staining1 Hi, i use to get some detach part of my cells during the process of IHC! Does any one recommend to incubate my cells prior embedding with sucrose?Try using these slides: (https://uk.vwr.com/app/catalog/Product?article_number=631-1349 I have answered a similar question here regarding wax sectio... [more]
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Answer added in Signal, Image and Video Processing131 Which language is the best for image and video processing?open cv , c++, vc etc and Matlab is also good tool for image/video processing
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Answer added in Cognitive Neuropsychology14 What are the best tests to use when trying to measure peak cognitive performance?Sorry, I missed your reply to other questions, clarifying your design. Some of my suggestions are still relevant, however.
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Answer added in Applied Linguistics300 Are there any languages that appear to have no grammar?@Klaus Have learned four languages wfrom age 0 to age 16 , easily without pressure now over 55 i try to learn bulgarian, german, and russian wow I f... [more]
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Answer added in Applied Microbiology1 TA cloning failure with pGEM-T easy vector !!
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Answer added in Gravitational Physics271 Can gravitons be discovered?Matts Roos - you stated: "Individual galaxies are usually not very efficient in producing strong lensing effects, galaxy clusters and superclusters do... [more]
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Answer added in Heat Transfer7 The influence of sample size on the result of glass transition temperature of glass using Differential Scanning Calorimeter,Dear Victor Ponce I have found a few papers that use Differential Scanning Calorimeter (DSC) test on fluoridate glass for example http://www... [more]
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Question asked in Embedded SystemsNew I want to Analyze Pipeline efficiency, Can you suggest me bit easy way? Also some links to download Object oriented C++ code.Friends, I am not so good in Programming, I want to Analyze Pipeline efficiency, Can you guide me bit easy way? how I do it? Suggest Any Simulator e... [more]