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Answer added in Fungal Biology4 I want the fungus cryopreserved at -80 ° C, but I do not know what is the concentration of glycerol used ?I tried diferent glycerol concentration and I agree with Linda Hanson in the most of the case 10% is ok,
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Question asked in Neurological DiseasesNew How is the synchronization in neuronal network for healthy subjects?There is nonlinear synchronization in the different brain region and it caused fast information propagation. There are both local and global synchroni... [more]
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Answer added in Architecture55 Specific tools to evaluate the design of urban public spaces?Fortunately There is a huge amount of software. For analysis use any of the below. Most are free. http://en.wikipedia.org/wiki/Spatial_network_analys... [more]
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Answer added in Cell Culture2 General Question..we can use 60% alcohol also to decrease rate of evaporation. That will be more cheaper. Even in absolute alcohol, we will be able to dissolve chemical... [more]
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Answer added in Bioinformatic Tools2 what do the letters in front of NCBI mRNA transcript sequences representThank you Daniel for you answer. I finally know! Do you know perhaps know if there was any differences in the methods used in the gathering of gene ex... [more]
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Answer added in Biology3 Does science contribute to "life" more so than religion, philosophy, culture, literature, and the arts?Very closely related to your question, there recently was an article published by Natalie Wolchover titled, "Science & God: Will Biology, Astronomy, P... [more]
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Answer added in Wildlife Management2 When does the sixth mass exctinction started ?There is an ongoing debate about whether current extinction patterns actually reach the level of a "mass extinction", as compared to previous mass ext... [more]
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Answer added in Chromatographic Techniques22 Which protein purification equipment is best for developing purification methods for proteins that will be used in FDA clinical trials?Cobus, The other route to think about is purchasing a used system. The way the GE systems are made a used system should be as good as new, relatively ... [more]
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Answer added in Heavy Metal Pollution1 I am establishing a journal of trace elemnets.......Is any body interested to join us as a member of editorial board.What are the aims and scope for the new journal?
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Question asked in Examination QuestionsNew How can I create ulcer with H. pylori on rats?I want create ulcer with Helicobacter pylori on rats. But I find a few article on rats and H. pylori. a lot of research about on Gerbils or mouse. Why... [more]
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Answer added in Adolescents’ Self-Concept: Understanding The Role Of Gender1 How do one publish with you.Yes, I publish articles but mainly science-based research. Publishing article involves systematic and painstaking arrangement of your reasearch outcom... [more]
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Question asked in SAS statistical softwareNew I need to do a competing risks analysis using SAS version 9.2.It will be with PROC PHREG. Does anyone have sample code for competing risk analysis?
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Question asked in SAS statistical softwareNew Time dependent covariates code in SAS.I need to add a time-dependent covariate into a Cox regression in SAS version 9.2 (PROC PHREG). Does anyone have sample code for this?
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Question asked in Mass SpectrometryNew Do urine proteomics have the potential to diagnose Kawasaki disease?Http://acceleratingscience.com/proteomics/urine-proteomics-may-have-potential-in-diagnosis-of-kawasaki-disease/
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Question asked in Heat TransferNew Measuring heat on the surface of a machineI would like to measure heat on the surface of a machine. I would like to know what kind of material is good at transferring the heat and can be magne... [more]
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Answer added in Power Electronics6 Why are we not considering the total output voltage in Sine-PWM Inverters?Hi everyone, First of all, I thank everyone who are contributed in this post. I apologize for any confusion caused because of the fact that my ques... [more]
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Answer added in Oral Medicine36 What is a safe and effective medication to use for severe recurrent and idiopathic oral ulcers in medically fit patients?Thalidomide as it is a reserve drug, and to be used in dentistry it is also far flung option keeping in mind the serious side effects as Majda pointed... [more]
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Answer added in Microbial Molecular Biology8 Does anyone have a good protocol for DNA extraction from deep sea sediment?However, if you decide to use your own method I would use a standard Tris/EDTA/NaCL/SDS extraction buffer and bead beat (zirconium beads) your sedimen... [more]
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Answer added in Statistical Analysis10 Which is the best statistical program for data analysis SPSS or SAS?I haven't experienced SPSS crashing with large sample sizes but it does have limitations compared to SAS. In my mind, SPSS language is a bit less rob... [more]
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Answer added in Tumor Immunology13 Optimizing CFSE proliferation assays for T-cells from fresh mouse spleen?I am not used to work with mouse spleen cells. i work with human lymphocytes (PBMC). Usually, I label lymphocytes (or PBMC) ten milion of cells/ml wit... [more]
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Answer added in Social Theory8 Is it possible for one person's habitus to become another person's field?Habitus should not be conceptually treated, or being conceived in terms of, field. Habitus is a pattern of an actor doing things habitus ( akin to Ari... [more]
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Answer added in Process Safety1 Scaffolding safetyFrom your question it seems you want to perform hot work in a confined space. The issue with the scaffold is not such a problem if it is properly cons... [more]
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Question asked in Fisheries ManagementNew Someone knows a fish population that was administered by more than one stock, no biological justification???In Chile, has been demonstrated (genetics, morphology, parasites) that the population of patagonian toothfish population is a single unit, but has bee... [more]
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Answer added in Botany2 Any idea what this might be?Close-up of 'spore'
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Answer added in Text Mining1 Does there exist such spell-checking system that is able to detect not just incorrectly written words, but also syntactic errors?I AM SURE you are aware of the imperfect Microsoft Word spelling and grammar checker. It can, of course, not only find misspelled words but also gramm... [more]
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Answer added in Soft Computing174 Statistical significance vs. Biological relevanceThis problem is a great one, every biostatistician has seen many times. Biological and statistical significance do not signify the same thing. You can... [more]
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Answer added in Molecular Biology4 How can I construct a vector to express a human protein for commercial use?You can certainly buy it, but if it is covered by patents, you will not be able to commercialize it without entering into a licensing agreement. You'... [more]
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Answer added in Odds Ratio8 Why is the odds ratio THE outcome measure in case-control studies?The rare-disease assumption is not needed when estimating effects from case-control studies. When e.g. samling controls longitudinally from the source... [more]
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Answer added in Navigation20 What novel ways are there for robot navigation?Actually there might be some situation in which the robot moves under some chair, table or some other furniture and not able to look at the ceiling. I... [more]