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Answer added in Statistical Analysis3 How count data on species be analysed, i am using R program?Hi all, The way you present it (analysing count data along environmental gradient, here elevation), I would suggest that you look into generalized lin... [more]
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Question asked in Computational Fluid MechanicsNew Master CFDI want to master CFD but I don't know how to start could you help me ?
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Answer added in Enzymology5 Can lactic dehydrogenase catalysis phenyllactic acid to phenylpyruvic acid in present of NAD+?It looks like the lactate dehydrogenases from 2 or more species ( P falciparum and Lactobacillus helveticus) can utilize the phenyl substituted substr... [more]
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Answer added in Statistical Analysis4 Goodness-of-fit test for the Von Mises-Fisher distribution?Woow Balázs! I don't know what to say, thanks a lot! However I have tested the code and there is something that is not working. The test passes also ... [more]
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Answer added in Systematic Reviews2 What is the main advantage of using the "mean differences adjusted for baseline" over the commonly used "mean differences" to analyze effect size?Dear Eik, thank you so much for your enlightening answer! Right now, I'm conducting a systematic review with few trials (although all of them are ran... [more]
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Answer added in experimental physiology3 Femoral artery catheterization in ratshttp://www.jpharmacol.com/temp/JPharmacolPharmacother32172-4186747_113747.pdf check it:)
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Answer added in Real-time PCR2 What to use as a control for Quantitative Real Time PCR for Human and Mouse cell lines? There is some controversy on which to use: L32, GAPDH, etcThanks Gowri. I have heard that too. Just some people whom do QPCR tell me not to used GAPDH or Beta Actin because the RNA expression levels vary in... [more]
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Answer added in Differential Equations9 Does a 3-D chaotic system (flow) with only a curve equilibria or with only a surface equilibria exist? Is that possible?Sajad, that is interesting. I didn't think of that (obviously)!
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Answer added in Epigenetics5 Design of Methylight RT-qPCR primersFor in silico checking of methyl-specific primer specificity, this site is quite useful. For help in deciding inputs, just hover your pointer over ea... [more]
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Question asked in Plant Tissue CultureNew Is somebody have experience with plant in vitro culture on acrylamide gels?I would like a gel that is stronger than agar, comparable to gelrite, without the vitrification trouble of gelrite, and that stay solid for a long ti... [more]
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Answer added in Protein Phosphorylation7 Protease/phosphatase inhibitor cocktail, which is the best?Hi, Sergio, Roche inhibitors are known to be the best among others. All my colleagues recommend these tablets too.
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Answer added in Wireless Networks2 Network Optimization to Optimizable protocolsPlease google "Software Defined Networking"...maybe it will answer a few of your questions! ;-)
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Answer added in Spine Surgery48 How can we improve the spine research projects?Dr. Wingerden. Yes, I agree :)
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Answer added in Molecular Markers6 Can I do a PCR succesfully without the last elongation stage?Yes of course Lebogang, this last step is sort of voodoo anyhow. It is not the last step that decides on success. It's more all the steps before! I ne... [more]
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Answer added in Tumor Immunology21 Optimizing CFSE proliferation assays for T-cells from fresh mouse spleen?Hi Fabienne, As we can see from the answers above there are many ways to do this. I work mainly with NOD and BALB splenocytes and have found that diff... [more]
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Answer added in Delusion34 What constitutes a "bizarre delusion" that meets Criteria A for a DSM-IV diagnosis of schizophrenia?Hi William, I also like your definition. However, is your belief still bizarre if 90% of people in your culture believe in Aliens who regularly rep... [more]
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Answer added in Thermoregulation7 My research deals with the effect of different corn particle size in the diet of naked neck laying hens on the quality of eggs and thermoregulation.Thanks for the corn particle size details. To find out the reason for poor shell quality, I would focus on two factors; calcium sources & level and fe... [more]
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Question asked in Molecular Biological TechniquesNew Does anybody know of a Inter-alpha trypsin inhibitor (ITIH1 or ITIH2) neutralizing antibody?Need to neutralize/block ITIH1 or ITIH2 present in FBS.
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Answer added in Instructional Design9 Do interactive whiteboards change the way we learn and what role do mental models play in the process?The study that we carried showed the importance of sharing the interactive whiteboard with the students. The interactive whiteboard is not the exclusi... [more]
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Answer added in Cholecystectomy3 Do you check the patient as routine for the Rome II or III questionnaire criteria before an elective cholecystectomy?The latter sentence cited from Eriksen JR, Boel T, Schulze S, Kristiansen VB. / Hvad sker der hos personer med galdeblaeresten, hvis de ikke far foret... [more]
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Answer added in Protein Purification4 how to go about purifying a his-tag protein, that needs a reducing agent, using a Ni column?Thanks Kai I was thinking of doing that. Will start with 5mM BME and see what happens.
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Answer added in Concrete Technology2 Specific Heat of concreteIf you measure thermal diffusivity with a transient method and thermal conductivity with a static method, the calculated result of specific heat value... [more]
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Question asked in Lab experimentsNew Can you please tell me how to viably freeze mouse cells from spleen, lymph nodes and BM? Thank youAfter mashing spleen and LN through a mesh, wash cells, counted cells and resuspended in the appropriate volume of 90% FCS and 10% DMSO, placed in Mr ... [more]
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Answer added in Islam33 The Impact of Culture and ReligionWith all due respect to all, the reason if any that some believers do not adopt the reproductive health advise from their religious vendors is that it... [more]
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Answer added in Heat Transfer1 The influence of sample size on the result of glass transition temperature of glass using Differential Scanning Calorimeter,sample size definitely affect the thermal parameters of the sample being sudied through DSC. excess quantity of sample will take long in completing t... [more]
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Answer added in Satellite Image Analysis2 Please I need 2010 Landsat ETM data with path 190 row 055 (bands 2, 4, and 7), any help please.I download it in ten minutes , do you have any ftp site?
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Question asked in Animal GeneticsNew Hi friends, I'm using ARLEQUIN 3.5. I'm getting negative Fst values. What does a negative Fst value tell?Population genetics
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Answer added in Gravitational Physics258 Can gravitons be discovered?I apologize -- I missed some of the messages discussing this issue further until just now. I'll try to address some of those points as soon as I can.
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Answer added in Patch Clamp12 What are the simplest electrophysiology experiments one can perform in the lab, for undergaduate students?In my experience, the cheapest experiments in electrophysiology can be performed with extracellular field recording. only nees stereoscopic mycroscope... [more]
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Answer added in Instructional technology225 Has digital information/communication technolgy increased or decreased learning efficiency in the classroom?Is this the right question to ask though? Isn't it like comparing apples with oranges? I often get asked a variant of this question and struggle with ... [more]