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- 3What selectable markers can one use for bacteria other than antibiotics for various purposes?
Specify for transformation,curing of plasmids,for pseudomonas,E.coli etc.?
Selection of markers depend on your study purpose and design, there are several visible / detectable biochemical characteristics, resistance to dyes, heavy metal salts, growth patterns etc. To read more you can see chapter 6 & 8 of the book at the following link.
- NewHow can you check if the DNA template used for PCR was contaminated or not?
The PCR outcome turned out negative and two reasons were suggested:
1. DNA polymerase used was expired
2. DNA template was contaminated
is there a possible experiment to confirm these two hypotheses?Following
- 8Can anyone recommend an encryption simulator?
Can anyone suggest me an encryption simulator?
gm respected AR Reddy sir,
sir i want to start my research work so from where to start in this area of developing and simulating performance.
sir can u guide me in this regard.
my email id is firstname.lastname@example.org.Following
- 1How can we simulate the Hodgkin & Huxley model by Matlab code and simulate action potential?
any details for answering is in the link below.
Hi, I'm not familiar with this kind of simulation but I've saw two very interesting examples on the internet.Following
- 2Anyone familiar with the immunofluorescence staining of MHC-II in dura mater?
Hi, all. I am staining of MHC-II-postive cells in the dura mater of mouse. I find that the MHC-II-postive cells contain two cell morphology, the rounded and the branched, please see the green cells in the figure of attachment. I think the cells contain lymphocyte and macrophages. But now i would like to distinguish the two types of cells using different immune makers？ Anybody can tell me?
Thank you very much!
Thank Kritika very much! I not sure whether there are only two APCs in the dura mater. I have double-stained for MHC-II (green) and CD11b (red) (a maker for macrophage), however, only few duble-stained cells were detected, see figure 3. I also stained for CD3e for the T-cells, but not stained for MHC-II because the two antibodies are made in same animal (Rat). Recent a data confirmed that few MHC-II-positive cells express CD3e, see figure1 and 2. Could you send me some researches about CD14 or CD64 staining for Macrophages (immunoflurescence method). Thank you again.
- NewIs there any guy works on piezoelectric Zno thin film for bulk acoustic resonator?
I am working on bulk acoustic resonator so i want to deposit Zno as a piezoelectric thin film.Following
- NewHow can i use different version(llumina beadchip) of maker position?
HI, nowdays i use illumina beadchip for GWAS analysis.
I have 2 type chip. It' s bovinesnp50_v1, bovinesnp50_v2.
But it has different chromosome, maker position in the SNP_map file.
So, How can i use different versions.
Can i imputation from bovinesnp50_v1 to bovinesnp50_v2.
If I can imputation it. After imputation, how can i use different version of maker position?
i wanna know answers. Have a nice day : )Following
- 2What is the best way to define promoter targets of two (or more) different transcription factors?
I'm performing some (very basic) bioinformatic analysis of our gene of interest, part of which is looking at the different transcription factors that may regulate it using the ENCODE Regulation track on the UCSC Genome Browser.
One particular question I'm interested in asking is: what promoters in human cells bind the same set of transcription factors as my GoI? In more specific language, which genes in the genome have a strong ChIP signal for two (or more) defined transcription factors within, say, 3kb upstream of their transcriptional start site.
I'm very new to this kind of whole-genome dataset analysis and as a result am a near-novice; any advice anyone could give on how to do this kind of analysis in a reasonable time would be greatly appreciated!
I'm expecting to need to download various genome viewer(s) and or datasets etc., but at largely at sea regarding: a) what best to use for this and b) the method/approach or coding required to e.gh. define the TSS distance and the requirement for double-positive ChIP signals is beyond me.
Alternatively, if there is a useful programme/took/database for this that I am unaware of, please do let me know!
Thanks in advance for all help!
Great, thanks Joseph! To be clear, I do know which TFs I'm keen to look at - but just wanted to try a couple of different pairwise combinations.
Just to clarify, is BEDTools a standalone programme or an extension to something like the UCSC Genome Browser? And is there a particular way of defining the, say, 3kb upstream? Or is this a built-in option to the programme? Apologies for the many questions - very much a "learning by doing" approach to bioinformatics!
- NewHow to design new component using FORTRAN in TRNSYS, kindly mention the step by step procedure please. I've already read the manuals?
I am struggling to create dll file of my newly designed component in fortran. Kindly send me suitable example please.Following
- NewWhat kinds of selective media can one use to confirm that a culture is, in fact, A. calcoaceticus?
I have a stock culture that I plan to use for further experimentation, but I first need to ensure that it is, in fact, A. calcoaceticus. I do not have the ability to get any type of sequencing done, other than performing PCR with primers that I can order. What are my options?Following
- NewWould you suggest good literature to differentiate mediator, moderator and interaction effect in social science research?
I am getting confusion.Following
- NewPlease tell me,How piezoelectric Zno thin film works?
I want to deposit piezoelectric Zno thin film for high overtone bulk acoustic resonator.Following
- NewHow to create new component using FORTRAN in TRNSYS, please mention the step by step procedure
It is also requested to please send documents/example file if anyFollowing
- 5Does anyone have any interesting references on memory distortion and eyewitness evidence?
How can trauma affect the distortion of a memory?
Any studies on brain activity?
The Neuroscience of Memory: Implications for the Courtroom
also (can not use directly) buy look to the references: https://en.wikipedia.org/wiki/Eyewitness_testimony
How does a traumatic event, such as witnessing a crime, cause ...
www.researchgate.net/ post/ How_does_a_traumatic_e...
(I'm a doctorate student as well as a law student)Following
- Mudassar Nazir added an answer in Microelectromechanical Systems (MEMS):6Is anybody working on piezoelectric thin films?Is anybody working on piezoelectric thin films?
Please tell me about the working of piezoelectric Zno thin film.Following
- 10What is the effect of intensive exercise on diabetic patients?
We want to find the effect of intensive exercise therapy on diabetic patients for reducing their blood glucose levels. Would like to know what kind of measurement tools can be used other than blood glucose readings, HbA1c levels.
In terms of long-term adaptations there is not much conclusive data. A typical characteristic in many people with T1DM is post-exercise hyperglycemia which is one of the top complaints in T1DM and one of top reasons why they don't stick to an exercise program. If high intensity exercise, not rare to see severe hypoglycemias in the middle of night. They take too much insulin to correct post-exercise hyperglycemia and they end up with hypos.Following
- 2From a spatial view: What can be considered the "landscape scale" ?
Something I always asked myself: In the field of Landscape ecology. How do we define and parametrize the "landscape scale" from a spatial view? What counts as a landscape and what is just the minimal covering area of all field sites? Are landscapes intrinsically defined by the social-ecological boundaries of humans that interact within them or is there sth. like a general theorem that defines the spatial scale (per ecological or environmental process) that a landscape can operate on?
My current view is that landscapes as such seem to be limited (in terms of spatial scale) by the question a researcher intends to address. Some processes (such as dispersal) act on a greater landscape scale including the surrounding, while others require a much finer focus towards the studysites. But this is obviously inherently biased by my (or others) perception as a human and other species might "perceive" a landscape not (only) in terms of processes.
Appreciate any cool references to seminal or review papers that investigated this.
I think the best approach is to consider what constitutes a landscape for the species or processes under study. The landscape impacting on an ant is generally going to be reduced compared to a deer for instance. We need to get away from defining landscapes based on human perceptions, and consider how the species we study perceive them.Following
- 9Is cervical traction is effective managaement for cervical spondylitis?
MRI impression : patient of cervical spondylitis with c5 c6 c7 disc herniation indenting subarachnoid space. Age 40 yrs, Spinal stenosis lumbar region 2years back treated with medicaion and exercise.
Now my query is about cervical traction can be given to this patient with interferential therapy followed by neck exercises. or only steroid medication effective for this condition.
Hi Vaner Koksal,
Its 12 mm according to the MRI.Following
- NewShould medical directors reconsider how hemodialysis facilities categorize, capture, escalate and analyze HD related hypotensive episodes?
Would standardizing coding utilizing accessory modifiers that better describe and discriminate dialysis-related hypotensive episodes improve risk prediction models, result in effective escalation and mitigation strategies, strengthen problem-solving approaches, enhance comparative research, and reduce repeat preventable harm to patients?
Validated research over time may prove that a more stratified hemodialysis hypotension classification will reliably predict impending doom, and effectively crack the code to prevent failure mode.Following
- 8What is minimum required sample size to compute Cronbach's alpha reliability for questionnaires/ scales?I am going to develop and validate women's autonomy measurement scale.
For correlating items, use correlation matrix among items.
Your second question is not clear, I think, you mean, three levels of rating. Whether three point or five point or more, no matter you can use cronbach's alpha for internal consistency test ...Following
- NewWhy did lemons evolve to be so acidic?
There's only one possible answer I can think of and I don't know if it's correct. This is one possible answer:
Lemons at first were slightly more acidic than oranges. Because of that they could corrode teeth faster than oranges. Any animal that corroded a hole in the enamel layer would be less likely to rear as many children and those that ate more lemons were more likely to corrode a hole in the enamel layer so natural selection selected for animals that slightly dislike lemon and prefer to eat oranges if they're available to eat. In addition to that, lemon trees were more likely to reproduce if fewer of their lemons got eaten and the ones with more acidic lemons had more of a tendency for animals to learn their lesson not to take a second lemon and eat it so natural selection selected for trees that produce more acidic lemons. That in turn caused even faster natural selection for animals that dislike lemons, which in turn produced faster natural selection for trees that produce more acidic lemons until it it starts approaching an evolutionary stable strategy where lemons are so acidic that it's so rare for animals to eat them that the biological cost of producing more citric acid is equal to the biological gain of having fewer animals eat their lemons as a result of producing more citric acid.Following
- 2Can I use the factors derived out of principal component analysis for conducting Pearson correlation analysis?
I have run PCA to reduce my metabolomic data to 5 meaningful factors. I was wondering if I could use Pearson's r to show a linear dependence between one of these factors and C-reactive protein. Does it make any sense?
Reducing data via PCA and then make a correlation analysis is a usual data analysis strategy.
Some statistical software allows you to include "supplementary" variables in the PCA. I guess that it would be usefull in your case ... so I suggest you to explore that option.
- 12Method development for determine VOC using HSGCMS?
I been running a method validation using Head space GCMS, but encounter some problem, now is there is high saturation of chromatogram at the start? what else can I improve and have a proper peak?
Other comment also appreaciate.
Attached with all the setting and chromatogram
Before I enter next phase, I encounter some problem and currently asking the GCMS engineer to help me,
When I using the sim mode, there is totally no peak come come, I don't know why. But if I use scan mode there will be peak come out, i still waiting for engineer solution for it.
I think our column and setting is different, that why eluent time is different for benzene.
Btw are you using the same method for testing? in what sample? Thank for the help to remove the methanol peak, scan from 40 really help to remove methanol peak.
- 1Streetlights r deliberately manufactured to provide illumination with reddish color.Based on EMR and spectral reflectance relationship suggest why?
please answer with reference..
Looks like these lamps are very energy-efficient, check Wikipedia:
- NewCan anyone suggest me a good co culture model for n2a and glial cell line?
Im trying to establish a co culture platform for N2A and glial glial cell line for neurodegenerative disorder study..
I see some papers have directly cocultured cell lines.. but I need a system where I can control the cells seperately but have them in same environment.
I would appreciate any suggestions.
- 14Can we use Structural Equation Modeling (using AMOS), with small data sample (n = 40) ?
I have a data set of 40 participants, and I want to develop an model using SEM, can we use Structural equation modeling with this small data set (n = 40), if all recommended GoF are satisfactory.
If you know please answer / reference.
The sample size is too small. In addition, there would be difficulty with external validity based on whatever results you find whether using SEM or Smart PLS. Lastly, it is doubtful such results would be publishable.Following
- 4Vascular surgery: What is the energy needed to obliterate a great saphenous vein using EVLT 1470nm? In total J ? and J/cmAnd the energy needed using 980nm laser ?
I've been using radial fiber since October 2009. More recently, the double ring radial fiber. I use 40 to 50J/cmFollowing
- NewReference oil used in Oil and Grease in FTIR determination?
Now I develop the method for Determination of oil and grease by FTIR
After I search through some reference, I notice the reference oil use in calibration standard is different to each other:
Method 418.1 Hexadecane, isooctane and chlorobenzene
EPA 5520C isooctane, hexadecane and benzene
Hach 10056 Stearic acid and hexadecane
Do I know any different? i know hexadecane is aliphatic and isooctane is branded and benzene is aromatic, but I don't understand why HACH using only aliphatic alkane for calibration? Is it any specific reason?
Hope someone can help me.Following
- 2What are the best opensource tools for packet capturing in network research. The aim is minimize packet losses experienced in wireshark(50%)?
Different solutions have been employed but packet loss ratio is very high. The best up till now has been wire-shark. Kindly guide as to how we can increase the capture ratio and what software are commonly used in the community for this purpose.
Thanks in advance for your help.
Can you give more details on what you are looking for? It sounds like your Wireshark causes packet loss during capture, but I don't think that is likely. The software just puts a network interface in listening mode and receives all packets, even packets that are not addressed to your machine. It is not a computationally expensive task, so I can't think of a reason Wireshark should cause losses. At least other software will have the same problem, they probably also use the standard libpcap to capture the packets.Following