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  • Nathan Weatherdon added an answer in Pedophilia:
    Can anyone share studies, author or any information about pedophile, pederasty in the social realm?

    I'm doing a qualitative research about pedophilia and pedereasty in my region and i need help connecting theoretical information about my topic, so i would appreciate if sombody helpme, maybe not a complete study about but an author, or an article, etc. Greetings!

    Nathan Weatherdon · Nathan Translates

    I would be half inclined to wonder what would turn up if you could interview people who had done time for such awful misdeeds, to probe for previous political views and potential brainwashing experiences. For real.

  • Chaojun Tang added an answer in Red Blood Cells:
    Is there any method for in vivo labeling of red blood cells in mice?

    Specific labeling for RBCs.

    Chaojun Tang · Soochow University (PRC)

    Many thanks!  I know the transgenic model in zebrafish not in mice. I will check it. And I will check with the glycophorin A antibody as well.

  • Rama Kant Dubey added an answer in Rhizosphere:
    What is the difference between antibiotic and pesticide (Bactericide)?

    I would like to know the difference between antibiotics and pesticides. I guess the term pesticide is applicable only to plants. Isn't it? in addition pesticides are applied on surface of the plants or seeds or applied in rhizosphere. Whereas, antibiotics are given orally or intravenously to human beings and animals. Am I right? Please answer.

    Rama Kant Dubey · Institute of Environment & Sustainable Development , BHU,Varanasi,U.P.

    Dear  Senthilarasu Gunasekaran,

    Antibiotics are microbial product use to suppress/kill the other microbes hosted within human as well as the plant body.

    Pesticide are  synthetic/chemical compounds generally used to control the plants specific pests.

    Bactericide specifically used as a killing agent for the Bacteria only. 

  • Nathan Weatherdon added an answer in Online Surveys:
    Online survey tool that allows you to re-contact respondents

    Does anyone know of an online survey tool that can be used in longitudinal research re-contacting the same participants several times.

    Nathan Weatherdon · Nathan Translates

    Survey monkey can do that. But I struggle to think of how to maintain anonymity in the process.

  • Nathan Weatherdon added an answer in Social Media:
    Impact of e-marketing and social media on SME's

    Hi..given limited studies on evaluating the impact of emarketing and social media on SME/SBE's is it really neccessary to have a theoretical framework to build the study on?.

    Nathan Weatherdon · Nathan Translates

    Each part of your theoretical model should be justified according to analogous research, or alternatively you can explain why you think they are wrong and testing for something different.

    If you don't enter with a strong theoretical model, justified using existing analogous research, then you run a very high risk of trying 20 models and getting a result that is 95% likely to pass statistical threshold by random chance.

    Anyone can construct a dataset and pick it apart until you find a p < 0.05 somewhere, somehow. That's why you need to justify it theoretically first. Justify the theory before crunching numbers so you don't find yourself in a situation of mining the data and arriving at results with fake statistical certainty. imo

  • Anthony Nash added an answer in Correction:
    How should one go about comparing the difference between two geometry minima in Gaussian


    In short I am getting a bit over whelmed with the difference in nomenclature used by the Gaussian output, text books, and people's suggestions.

    I am trying to pin point the most energetically favourable conformation between two geometry optimised structures (A and B). Both structures are in a minumum with no imaginary frequencies. What 'value' would be the best to report. Here is an example of where I am looking in a Guassian log file after a frequency calculation.

    Structure A)

    Zero-point correction= 0.549237 (Hartree/Particle)
    Thermal correction to Energy= 0.580114
    Thermal correction to Enthalpy= 0.581058
    Thermal correction to Gibbs Free Energy= 0.486170
    Sum of electronic and zero-point Energies= -1484.656742
    Sum of electronic and thermal Energies= -1484.625865
    Sum of electronic and thermal Enthalpies= -1484.624921
    Sum of electronic and thermal Free Energies= -1484.719809

    Structure B)

    Zero-point correction= 0.549667 (Hartree/Particle)
    Thermal correction to Energy= 0.580703
    Thermal correction to Enthalpy= 0.581647
    Thermal correction to Gibbs Free Energy= 0.483468
    Sum of electronic and zero-point Energies= -1484.647529
    Sum of electronic and thermal Energies= -1484.616493
    Sum of electronic and thermal Enthalpies= -1484.615549
    Sum of electronic and thermal Free Energies= -1484.713728

    I should then (hopefully) be able to report on the difference of X (my question) between structure A and structure B.

    Thanks for your help. I'm still very new to ab initio and I am far more used to Free Energy MD calculations.

    Anthony Nash · University College London

    Thanks for clearing that up Alexander. 

  • Ramis Örlü added an answer in Gradient:
    Could anyone tell me what is the best method to estimate turbulent boundary layer wall skin friction for wind tunnel experiment?

    I want to use Clauser method to predict zero pressure gradient boundary layer characteristics in our wind tunnel to be used for a baseline in adverse pressure gradient turbulent boundary layer experiment. I tried to used wall skin friction for pipe as suggested in many books for an assumption but the result seems to have a margin of error.

    Ramis Örlü · KTH Royal Institute of Technology

    If the aim is to determine the skin friction from available data, there are a number of methods besides the Clauser chart method that could be used. Few are referenced here:

    Örlü, R., Fransson, J.H.M. & Alfredsson, P.H., 2010. On near wall measurements of wall bounded flows--The necessity of an accurate determination of the wall position. Progress in Aerospace Sciences, 46, pp.353–387.

  • Zamin Mamiyev added an answer in Materials Science:
    What are the synthesis parameters difference among nanostructures of same kinds of materials?

    Materials Science

    Zamin Mamiyev · Institute of Physics of Azerbaijan National Academy of Sciences

    Dear P.Ahmad 

    It depends on the synthesis technique. changing of pH, precursors amount, temperature give possibility to manage distribution and size of nanoparticles on matrix or substrates.

  • Does anybody know of any qualitative research exploring UK attitudes to the welfare system?

    (Whether immigrants should get benefits and why/why not for example.) I expected there would be some (particularly given how much is known quantitatively through things like the British Social Attitude Survey and the topicality of this issue) but I am coming up empty on google scholar. (This may partly be a problem of rather "generic" search terms like attitude, welfare, immigrants and so on.)


    Nathan Weatherdon · Nathan Translates

    A priori, I would guess that such a politically laden question of study will be dominated be low quality ideologically driven research. I would immediately lend credibility to those researchers who demonstrate awareness of sensitivity of results to the ways in which questions are framed and are openly self critical in this regard.

  • Alam Khan asked a question in Conflict:
    Among these three internal conflict, external conflict and terrorism which one has more effect on economic performance?

    Economics, political science

  • Raman Jeet Singh added an answer in SimaPro:
    Can we do life cycle analysis (LCA) without the use of Simapro or any other software?

    Life cycle analysis is a tool which estimates environmental indices (Eutrophication potential, acidification, global warming potential) of any input added to ecosystem.

    Raman Jeet Singh · Central Soil and Water Conservation Research and Training Institute

    @ Stefan and Katia

    If we have our own database i.e. inputs used in crop production then is it necessary to use Ecoinvent or USDA database. If your answer is no, then how we can add that data to OpenLCA software. 

  • Tomo Saric added an answer in Incubators:
    Do you have any tips for hMSC culturing and subculturing?

    Dear All,

    I have recently started working with hMSC's (Bone Marrow-Derived). My issue is when I culture from stock they seem okay, but once I sub-culture it I see lot of cell death and debris. Also several of the live cells have vacuoles because my experiment requires a study on actin cytoskeleton of hMSC's. I feel the presence of vacuoles will not help my aim. 

    Any suggestions are welcome.

    Culture conditions:

    Gibco DMEM(low glucose)+10%FBS (GIBCO) +PE/ST in an incubator 37 deg Cel, 5% Co2.

    Culturing currently in 10 cm dish. 

    I need good cell numbers at least 5x10•5 or more per ML

    Tomo Saric · University of Cologne

    You are welcome, Sri. Good luck with your experiments!

    Best, Tomo

  • Stability analysis of Smith predictor controller wrt time delay of plant?

    I would like to analyse the stability of a closed-loop system in which the controller is built around a Smith predictor. I am interested in particular with the stability analysis with respect to uncertain time-delay in the plant. Thank you.

  • Paul Louangrath added an answer in Data Analysis:
    Do I need to test for the validity and reliability of an instrument while my research requires me to use secondary data only?

    Am analyzing on qualities of accounting information by using auditor reports.

    Paul Louangrath · Bangkok University

    ISSUE: Validity of an instrument & reliability of an instrument

    The issue of this question requires a careful reading. The questioner is not asking about the validity or reliability of the “data used in the audits.” She asks about validity and reliability of the “instrument for the audit reports.” It is the “audit reports” that are the secondary data, not the financial statements, but the reports themselves. The instrument is the “what produce the audit report”---thus, the instrument is the audit methodology.

    INSTRUMENT: The instrument of this case is the “audit methodology.” The data that the questioner deals with are the reports generated or produced by the auditors. The Audit report is the data. The income statements used in the audit report are not at issue. To rephrase the question: (i) Is the instrument valid, i.e. does the audit methodology produced what it intended to produced? and (ii) Is the instrument reliable, i.e. if an independent auditor engages the audit using the same method, would they produce the same result?

    STANDARD OF PRACTICE: If the accounting or financial statements used for the audit were in compliance with IFRS, IAS or GAAP, then the issue of “accounting data reliability” becomes moot. Assume that that IFRS, IAS or GAAP was used. Accounting data reliability is not an issue. Are the questions of validity and reliability test still an issue in this case? The question presented is about “validity and reliability tests of the instrument.” What is the instrument? The data is the audit report. The instrument is the audit methodology used to produce the audit report (data). This different language points to different points of analysis.

    SUBJECT MATER: The subject matter for the analysis is not the original accounting data or financial statements generated by the companies. The focal of the analysis is the “accounting reports” themselves. Note the use of the term “reports”---plural. This means that the audits may have come from many auditing firms. Different auditing firms may use different auditing methodology in completing the audit. Therefore, the issue of validity and reliability of the audit result become legitimate questions.

    VALIDITY: Validity is the test for precision. Does the data (observation) hit the target as predicted or hypothesized? In this case: does each accounting report confirm the asserted statements? If the audits had been signed off as “passed” audit, then this issue becomes moot because the observed data (reports made by the firm) and the theoretical data (standard of reviewed imposed by auditor) matched---hence the auditor signed it off as passed, i.e. confirmed the asserted statements in the financial statements by using the generally accepted principles and methods in auditing.

    RELIABILITY: Reliability is a test for reproduction. The issue is whether if other auditors using the same accepted principles and methods in auditing would produce the same result? If subsequent review by independent auditors under generally accepted principles disagree that the result should have been different then the audit reports does not pass reliability test. Hence, the “audit reports” are the “data” in this case, it is then said that the data does not pass reliability test. Note it is the audit report that is the data, not the financial statement. Then the researcher must look into the sources of disagreement, i.e. auditing method or different treatment of certain categories of event in the accounting at different time period that would have produced different result.


  • Huong Ha asked a question in Cellular Structures:
    Have you seen a quenching effect of zinc ionophore on fluorescent intensity?

    Hi all, 

    I am using the ionophore (sodium pyrithione) from Sigma to do several experiments either with zinc indicator or with another fluorescent tagged protein. I am looking at both soma and small cellular structures like presynaptic boutons or postsynaptic spines from dissociated hippocampal culture from rat embryo. When the ionophore was used in soma imaging using FluoZin-3, we did not observe any quenching effect. Instead, there was a proportional increase in fluorescent intensity with increasing zinc concentration. We used 10x objective in this experiment. That was exciting. However, over the past few weeks, it has occurred to me that the application of  2 uM ionophore plus either 10 uM or 300 uM ZnCl2 seemed to quench the intensity of both the dye and the fluorescent protein when looking with high magnification objective (63x). Did any of you see this before? Would you have any suggestion for another ionophore?

    here is the link to the product that we are using:


    Thanks all!


  • Graeme Smith added an answer in Brownian Motion:
    What are the differences between Brownian Motion and Matlab's rand() command?

    please explain the difference.

    Any Rand command in a computer language is an example of a pseudo-random number generator. These do not actually create random values, because no one knows how to create real randomness, instead they approximate it by using relatively non-repeating strings of digits from a prime number. Brownian motion on the other hand may not be truely random either, but seems random because we don't know what the forces are that are acting to change the locations of sub-atomic particles.

  • Ahmad Taher Azar added an answer in MPPT:
    Can anyone help me with Fuzzy MPPT for PV systems in MATLAB?

    I tried modelling the fuzzy MPPT with E=dp/dv and CE=E(n)-E(n-1).

    I have some problems with the model I designed. I think the problems are associated with the saturation block and the memory block I added in. The model is not working properly. 

    I need help in this regard.

  • Dheerendra Gupta added an answer in RNA-Seq:
    Do anyone knows about which kit is better to use for isolating mRNA from total RNA for RNA-seq between Illumina and NEB?

    I am right now using NEB mRNA oligodT magnetic isolation module and took 5ug total RNA but after following the instructed protocol when I tried to quantify the mRNA I found almost nothing in the read, machine showed very low concentration which was unable to be detected.I don't know where problem exist my all six samples failed? Can anyone help me in this regard?

    Dheerendra Gupta · Institute of Life Sciences

    Yes I did the estimation of purified RNA it was around  2.5ng/ul i.e total RNA was 35ng in total volume of 15ul..Is this concentration sufficient for preparation of mRNA library? Or any other suggestion or protocol change I can follow for further preparation of more libraries..

  • Is there example of FCM and ANFIS working collaboratively in practice?

    Anyone have a clear practical example of how those two algorithms work together ? 

  • How can elements of either cooperative and non-cooperative game theory be applied to study either the internet of things or big data?

    How can elements of either cooperative and non-cooperative game theory be applied to study either the internet of things or big data?

    Shahab Shamshirband · University of Malaya

    These papers may encourage u to choose cooperative or non-cooperative game.

    1) http://www.sciencedirect.com/science/article/pii/S0952197614000311

    2) http://www.sciencedirect.com/science/article/pii/S0952197613000766

    3) http://www.sciencedirect.com/science/article/pii/S1084804514000666

  • How can the cost of soil conservation be estimated in terms of "payment of ecosystem service (PES)"?

    Soil serves us many goods and services while soil erosion affects these economic and ecological services. I am interested to know how these impacts can be quantified in terms of PES, if conservation measures is used to check soil erosion?

    Debashis Mandal · Central Soil and Water Conservation Research and Training Institute

    Thanks, Costantini for sending the paper.

  • Analyzing and detecting cloud anomalies using anomaly detection

    What methods/tools i might require and what will i be measuring for anomaly detection in cloud computing environment?

    Please guide  me how to approach this question.

    Thank you

    Shahab Shamshirband · University of Malaya

    The main question is that which type of anomalies do u want to focus in Cloud computing environment.

    I suggest you to find a type of attack or anomaly in cloud environment, then you may design a agent based architecture to detect the type of attacks.

    You can apply the simple method like Fuzzy Rule Based in your first effort . Later you can expand your methods with Game theory based IDS in cloud.

    Please see recent published papers.

    1) http://www.sciencedirect.com/science/article/pii/S0952197614000311

    2) http://www.sciencedirect.com/science/article/pii/S1084804514000666

  • Helen Wilding added an answer in Intervention:
    Can anyone recommend literature relating to the promotion of family inclusion strategies within mental health inpatient care?

    I am looking at improving the engagement and involvement for families/carer's within a treatment and recovery centre. We currently offer structured Family Intervention but my aim is to create a more person centred, stepped approach which can offer inclusion and interventions built around need.

    Helen Wilding · St. Vincent's Hospital Melbourne

    And a systematic review:

    Eassom, E., Giacco, D., Dirik, A., & Priebe, S. (2014). Implementing family involvement in the treatment of patients with psychosis: a systematic review of facilitating and hindering factors. BMJ Open, 4(10), e006108-e006108. doi: 10.1136/bmjopen-2014-006108

  • Rohit Upadhyay added an answer in Plagiarism:
    How much plagiarism is allowed for the acceptance of research paper?

    Does the research paper from own thesis is also considered as self plagiarism?

    Rohit Upadhyay · IIT Kharagpur

    You can opt for iThenticate or Turnitin softwares.

  • Jazeel Hussein asked a question in Galaxy:
    What are the dynamical information that can be derived from the rotation velocity?

    What are the parameters that I can get from the rotation velocity of the galaxy?

  • Is there any viable and innovative solution for the world’s food crisis and food-shortage problems?

    The earth’s population may grow by three billion in the next 50 years or so. The world’s ever-growing number of people points toward a greater demand for food in spite of the already-scarce resources. Famine, hunger and starvation are accordingly a major issue in poor countries worldwide! In your opinion, would the world’s food production/industry suffice to feed the hungry stomachs (given the land and water limitations/scarcity)? What are the world’s options in this respect?

    Thanks so much for your contribution!

    Shafig I. AL-Haddad · Princess Sumaya University for Technology

    Have you heard about “pinkhouses”

    This is a “new age” farming method, based on the fact that plants mostly need the red and blue colors of the spectrum to thrive. The more they get, the faster they grow. So vertical farmers are installing tracts of computer-controlled LEDs to maximize plant growth.

  • Vasuki Soni added an answer in Magnetic Field:
    How can I accelerate electrons flowing through a wire using a magnetic field?

    My motive is to speed up electrons in a wire using a magnetic field.

    What arrangement is preferred for such an experiment?

    Vasuki Soni · ABB

    Ok fine. Thats nice. I will explore more on this.


  • Ashok Behuria asked a question in LMS:
    Q) Can anyone explain me about the detailed procedure of converting lms into xlms algorithm?

    Please include detail explanation using diagrams