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ResearchGate Q&A lets scientists and researchers exchange questions and answers relating to their research expertise, including areas such as techniques and methodologies.

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  • Nissar Kuchay added an answer in Hospitality:
    What qualitative techniques will be best suited for Hospitality Research?

    What qualitative techniques will be best suited for Hospitality Research to Find out consumer preferences on Green initiatives and Why? Please share any study using qualitative techniques. Thank you.

    Nissar Kuchay · University of Kashmir

    You can use questioners with a range of qualitative answer options. which will allow the respondents to respond in a free and qualitative manner and could be later converted in to scores and weights and put to quantitative analysis through composite indexing as well.

  • H.-P. Schulze added an answer in Electrical Discharge Machining:
    How do I perform thermal erosion analysis on ansys?

    I want to analyse the tempreture of the plate after electric discharge machining on ANSYS.

    And how to apply temperature on a single point or particular area in ANSYS.

    H.-P. Schulze · Otto-von-Guericke-Universität Magdeburg

    Xiaobing has been correctly noted that we need to know in advance whether a single discharge or the areal removal should be considered.
    In ANSYS from a heat source to an active surface (plasma radius) is assumed. The energy density should be determined on the measured function curves of u (t) and i (t). For initial tests and linear simplifications or the average energy density can be recognized.
    Then you have to decide whether you want to simulate a constant plasma radius or a variable radius. You will find some solutions in the literature (see also H.-P. Schulze). The most complex is to obtain the correct parameters of the electrode materials. For a more accurate simulation they must also consider the melting and boiling enthalpy.
    Those are the first important prerequisites.
    For the calculation of ablation volumes (subsequent discharges) you can quickly Aachen under the literature of the WZL.

  • Ru-Jeng Teng added an answer in High-Frequency Ventilation:
    What is your current perspective on use of HFOV in children??

    There is a lot of concern regarding use of HFOV especially after the publication of the twin trials, OSCAR and OSCILLATE. Though these were adult trials, it has definitely dented the confidence on HFOV as a mode of ventilation for children with ARDS. 

    What is your personal practice? How do you look at the effectiveness of HFOV in  light of your clinical experience? When do you consider its use in children with ARDS?

    Ru-Jeng Teng · Medical College of Wisconsin

    We, at least myself, do not use HFOV as primary ventilator mode for ARDS. Permissive hypercapnia is the only strategy I will use. It all depends on my patient's response and I will attempt to switch to HFOV and determine which ventilator benefit the specific patient. 

  • Michał Bogdziewicz added an answer in Seeds:
    Why seeds of the same plant individual has different size?

    Hi,

    is there any work pointing the benefits of producing seeds of different size be the same plant individual? I find a work of Poulsen et al (2014) J Ecol (attached) about the benefits of producing dimorphic seeds (hard and soft). I wonder if we know something about other traits? 

    I do know that there is the trade-off between number of seeds produced and their size. But why seeds produced by one individual can vary so much? For example, in red oaks it can range from 1-5 g acorns produced by one tree. 

    Any (good) papers on the subject?

    thanks!

    Michał Bogdziewicz · Adam Mickiewicz University

    Thank you. In the mean time I found numerous papers dealing with that problem. As far as I can see this is largerly unasnwered question. For some species this is unpredictible environment, for some different part of plant that are resource-limited, for some amount of pollen that they will recive. This is really interesting!

  • Narayanan Nair Mohanan added an answer in Herbarium:
    Are there any priority wise uses defined for herbaria?

    We all know that globally besides having role in authentication and reference for describing new species herbaria played a critical role in studies pertaining to climate change, ethanobotany, ecology, DNA bar coding etc. Can any one tell me if there are any priority considerations in use of herbaria for various purposes? I shall also be happy to receive the articles which describe role of herbaria in documenting biodiversity.    

    Narayanan Nair Mohanan · Jawaharlal Nehru Tropical Botanic Garden and Research Institute

    Voucher specimens well prepared and deposited in an approved Herbarium is the only proof of identity for any scientific research using plants, from very basic to to most advanced. Do not underestimate the roll of Herbarium.

  • Neeraj Pandey added an answer in InSAR:
    Can anyone provide me with InSAR interferometric data?

    Can anyone provide me InSAR interferometric data having interferograms?

    Neeraj Pandey · Instruments Research & Development Estalishment

    Thanks very much Mr. Cesar.

  • Assss Dddd asked a question in Motion Estimation:
    Which algorithim is best for motion estimation in H265

    Hi I am implementing the HEVC on FPGA but I have issue regarding to logarithm used for motion estimation in HEVC as there are lot of algorithm available for motion estimation

  • K.M.Sajid Ali added an answer in Crop Science:
    Can any one suggest me any article in terms of Winter Wheat LD50 value for EMS?

    for Mutation Breading purpose

    K.M.Sajid Ali · Maharashtra Hybrid Seeds Company Limited

    0.3 to 0.7 is being concluded in various articles  with different temp as well as treatment  duration but more specifically I am looking for spring wheat.,

    @ Anupam Barh thanking you for your consideration, I have gone through the paper that you suggested, but the wheat verity which is being used in first link is summer wheat.

    "Looking for more specific reference, with spring wheat "

  • Arun Sharma added an answer in Anti Microbial Activities:
    How can I calculate the anti-microbial activity of nano-particles ?

    I have made Polymeric nano-particle loaded with anti-microbial drug (Levofloxacin), I want to perform anti-microbial study. As nano-particle is believed to give controlled drug release . Regarding the same how can I represent the controlled anti-microbial study. Kind Suggestions are welcomed.

    Arun Sharma · Jaypee University of Information Technology

    Thanks For your kind suggestions 

  • Wonyong Lee asked a question in CD4:
    CD4 T cell adoptive transfer and choosing CD4 mAbs?

    Hello there,

    I am trying many in vivo experiments and most of them includes CD4 T cell adoptive transfer between mice. But not many results show significant difference and now I am worried if the T cells I transferred might be defective.

    My concern is that anti-CD4 fluorescent monoclonal antibodies I am using for sorting CD4+ T cells might block CD4 signaling and inactivate T cells. In my lab, we have GK1.5 and RM4-5. Application notes on BioLegend website say they both can block CD4 T cell activation, or even deplete CD4 T cells.

    I hope anyone with experience in CD4 T cell transfer can answer if my concern is just or irrelevant.

    Thank you.

  • Gyu-Sung Cho added an answer in Enterococcus:
    Help with MIC for E. faecalis using tryptic soy broth. The only article I can find on this is Langfield, 2004. Does anyone know of other articles?

    Determining MIC for E. faecalis using micro-titer plate  

    Gyu-Sung Cho · Max Rubner-Institut

    Hi

    maybe check the CLSI M100 S24 release. there are some Information about E. faeclais MIC test and break Point.

  • R. Baby Suneetha added an answer in Spectrum Analysis:
    Are the adsorbent materials valid for taking UV spectrum analysis?

    The adsorbent materials with different concentration I took for Uv spectrum analysis, In that I got transmittance is increased when increased my concentration level of my additive. Actually adsorption is a surface reaction only. Then how it is possible to increase the transmittance value of adsorption materials. 

    R. Baby Suneetha · V.O.Chidambaram College, Tuticorin

    please be more clear

  • Abdullah Alhameed added an answer in Econometric Modeling:
    If have a model with higher R adjusted but with one factor insignificant and other model with lower R adjust but significant factor which is better?

    I have a econometric model to see how currency (USD) affect the ADRs returns in countries of Latin America.

    For two countries I have run the regression with two and three variables:

    The model with 2 variables: dependent variable: ADRs returns and independent variables: changes in currency rate and US market return

    The model with 3 variables: independent variable are:changes in currency rate and US market return and home market return.

    The thing is for model with 3 variables I have a higher R adjusted but  on variable become insignificant and for the model with 2 variable has a lower R adjusted but the two variables are significant. then which model i need to choose?

    Abdullah Alhameed · Al-Jouf University

    Why not try transforming all your variables into another unit root-free format e.g. 1st. differences or log-differences?

    Have your tested for serially correlated errors and unit roots or co-integration?

    However, regardless of the statistical significance of your right-hand variables, you should always lean toward keeping those variables suggested by economic theory.

    Good luck.

  • Artur Braun added an answer in Empowerment:
    What is the difference between discussion and discourse?

    Discussion and discourse are very important tools of communication and learning. They also help in promoting opinion-making in desired direction. These terms can also be seen as tools of involvement and empowerment. But the difference between these terms is not quite clear. I seek your help in this regard.

    Artur Braun · Empa - Swiss Federal Laboratories for Materials Science and Technology

    This is a very good question. I believe the "discussion" is the proper way to discuss this question. It cannot be a discourse yet because to me it seems not really established where a discussion is only a discussion or a discourse can only be a discourse.

    Habermas is associated with the term discourse and I believe discourse comes where implicit claims about the meaning of contents of communication are made, where opinions play a role. Whereas the discussion in my opinion (you don't see me contradicting here, do you?) may come from discussion partners who do not have a strong opinion on their opinion. I would say the discourse may go deeper in the topic than the discussion. Also I believe a discourse can be made by one person, whereas a discussion would suggest at least 2 partners.

    In the end discussion and discourse can be taken as tomatoes vs. tomatoes, a semantic difference only.

    Hopefully in addition to the discussion going to be made here about your question, you and some other experienced deep thinker may be able to produce a discourse over the topic.

    :-)

  • Kamalashwaran Sivasubaramanian asked a question in Alloys:
    How to characterize high entropy alloys from conventional alloys of same composition?

    high entropy alloys

  • Yuriy Pilgun added an answer in NIR:
    What fraction of container must be filled with liquid sample by NIR-measurement?

    What fraction of container must be filled with liquid sample by NIR-Measurement to get perfect spectrum?

    Thank you in advance

    Yuriy Pilgun · National Taras Shevchenko University of Kyiv

    Intensity of a spectrum is proportional to the length which light travels through the sample. If the sample "thickness" is too small, you will get bad spectrum with small and noisy peaks. But if the sample is too big, absorption will be too large and no light will pass through the sample, so you will get saturated peaks. It all depends on the strength of absorption peaks of particular liquid.

    I think you should find out which direction light travels in your instrument, and then try different sample "thicknesses" along this direction (there should be different containers for liquid) for your range of concentrations to understand which is best for your sample.

  • Varun Varma added an answer in GeoTIFF:
    How can I import a Matlab matrix in ArcGIS? Is it possible to obtain a DEM from a GeoTIFF, in ArcGIS?

    I imported a DEM from ArcGIS to Matlab (R15a) and I used it as initial matrix in a model I wrote. Now I have the final matrix representing the final surface after N model steps and I would like to import it back in ArcGIS. I tried to export it as a GeoTIFF from Map Viewer, after having built the X and Y geolocation matrices that Matlab requires. What I got is a spatially referenced 3 RGB channel image with 8 bit depth, that I imported in ArcGIS. My problem is that I need to have the DEM of the final surface in order to make comparison with the original one, and the GeoTIFF does not allow me to do that because values of pixels are not altitude values but number from 1 to 255. Does anyone know if there is a more convenient way to export a grid from Matlab to ArcGIS? Or maybe if it is possible to obtain, in ArcGIS, the DEM from the GeoTIFF?

    Varun Varma · Tata Institute of Fundamental Research

    I agree with Essayas. Try creating your output matrix as an ASCII grid and then importing it into ArcGIS. If you are unfamiliar with it, think of the ASCII file (*.asc) as a text file which has each pixel value in a tab separated format and importantly, has header information specifying parameters of the raster, such as numbers of rows and columns, x and y coordinates of the origin (of the layer), cell size, no data value, etc.

    See details of what an ASCII file needs to contain here - http://help.arcgis.com/EN/arcgisdesktop/10.0/help/index.html#//00120000002s000000

    A crude procedure (do this only if the output is small, i.e. file size upto a a few 10s of MBs) to make this file would be to save your output matrix data as a .txt file. Open it in a standard text editor like notepad or gedit and append the header info required (as outlined in the link above). Save as a .asc file and import in ArcGIS.

    For larger files, I would not recommend opening it in a text editor. But use Matlab or a similar environment to access the text file and append the header info at the start of the file, then save as a .asc file. I do not use Matlab so cannot elaborate on the procedure. But in python, a simple .append function does the trick.

    Once imported into ArcGIS you will need to define the imported layer's projection.

  • Ali Akbar Shaikhzadeh added an answer in Seismic Design:
    How can I measure the seismic capacity for a high rise building?

    how can i measure the seismic capacity for a high rise building

    Ali Akbar Shaikhzadeh · Ferdowsi University Of Mashhad

    The seismic capacity of any structure including high-rise buildings depends on the type of the seismic resisting system you provide. For high-rigse buildings there are some special provision you have to consider while designing. 

  • Govindan Selvakumar added an answer in Soil Fertility:
    Why don't we talk about microbial solubilization of micronutrients?

    The enigmatic role of soil microbes is gradually being de-codified towards better understanding of soil fertility transformations. We frequently talk of N-fixation , phosphate solubilisation , potassium solubilisation ..... but hardly debate about potential role of microbes including the AMF in improving the available indices of  micronutrients in soil with respect to micronutrients like Fe, Mn , Zn , Cu , Mo, B etc .The widespread deficiency  of these micronutrients is so ominous and of so far reaching consequences on both soil as well as plant health , in addition to quality of edible parts  . I invite the comments of our friends on the role microbes including the AMF in  transformation and availability of micronutrients across crops and soils.

    Govindan Selvakumar · Indian Institute of Horticultural Research

    While screening for Zinc solubilization, I would prefer to get the final stamp of approval from ZnO solubilization since the percent Zn is highest in this salt and the ability of an organism to release soluble Zn from this substrate would be the ultimate

  • Balasubramani pk added an answer in Hematopoietic Stem Cell Mobilization:
    How can I remove early stage erythroblast when isolating HSC from cord blood?

    I'm trying to isolate human HSC from cord blood. I use Ficoll to get  mononuclear cells and then perform lineage depletion,CD34+ seletion at last. Yet I found that early stage erythroblast as nucleated cells couldn't removed by Ficoll density centrifugation. Also they couldn't be remove by lineage depletion. For the worst, they express some extent level of CD34. So I get huge contamination of early stage erythroblast in CD34+ HSC. I know that Rosette form STEMCELL Technologies could remove those cells before CD34+ selection, besides that,any idea on how to remove these cells, like unique markers?

    Balasubramani pk · Cryo Stemcell pvt ltd

    @ Yu Zhou

    We diluted CB with PBS/EDTA (not for mobilized peripheral blood).

    Concentration of PBS/EDTA used in the process?

    We used Ficoll to get mononuclear cells as you mentioned. It is very common some red blood cells trapedin the Ficoll layer.

    So ParmLyse was used to lyse red blood cells.

    Volume of ParmLyse used and Time period of incubation?

    Sometime we treated the pellet twice. Then we used CD34 magnetic selection to purify HSC (Miltenyi system).  We did not  find ery-lysis step affect function (proliferation or differentiation) of the HSC.

    1. Concentration of PBS/EDTA used in the process?
    2. Volume of ParmLyse used and Time period of incubation?

    Thank You in advance !!

  • Shama Mujawar added an answer in Medicinal Chemistry:
    What is the best free software for QSAR and molecular docking?
    Apart from ArgusLab
    Shama Mujawar · RASA Life Science Informatics

    @Renjith Pillai for multiple ligand docking you can use AutodockVina

  • Sudhanshu Maurya added an answer in IPv6:
    Are there any methods to mitigate the vulnerabilities of IPv6 in cloud?

    I want to focus on IPv6 but don't know really how can I found a gap in cloud relates to IPv6 :(

    Sudhanshu Maurya · Jharkhand Rai University

    Visit the Links, it will help you definately:

    :https://www.sans.org/reading-room/whitepapers/detection/complete-guide-ipv6-attack-defense-33904

    https://www.sba-research.org/wp-content/uploads/publications/Johanna%20IPv6.pdf

  • Maureen F Legge added an answer in Physical Education:
    Is there any publication/research about diversity or masculinities using visual methodology in a sport or PE context?
    I know Hills' chapter in the Azzarito's book about visual method (Routledge), but is it the unique work abuot diversity/masculinities using this new methodology?
    Maureen F Legge · University of Auckland

    Can't help you there with a specific reference but I think it would make a great study. I have used visual methodology, with a colleague, to explore our teaching of outdoor education  which in New Zealand is a PE context. We look at the way we have used experiential learning in our teacher education for outdoor education.

  • Sharmila Shaik asked a question in Plasmids:
    How to store recombinants clones for a long time?

    How to store recombinants clones(storing a plasmid) for a long time?

  • Balraj Singh Gill asked a question in Triterpenes:
    Why triterpenes are less targeted to the PI3K/Akt/mTOR pathway?

    why plant and fungal triterpenes are less targeted to PI3K/Akt/mTOR pathway?

  • Narayanan Nair Mohanan added an answer in Plant Taxonomy:
    Does anybody know the name of this plant ?

    I need a common name or scientific name   or group to which it belongs

    Thanks

    Narayanan Nair Mohanan · Jawaharlal Nehru Tropical Botanic Garden and Research Institute

    Surely Araceae plant.   But the fruit looks  to me different from Anthurium polyschistum 

  • Mahdi Sharifi asked a question in Nonlinear Analysis:
    Nonlinear Analysis of Steel Frames using Abaqus without Axial force in Beam

    I am trying to model a simple one-bay steel frame using nonlinear analysis in Abaqus. I do not know how I should exert the lateral load and boundary conditions in order to avoid any axial force in the beam.

    It should be mentioned that:

    1) I turned on the Nonlinear Geometry (NlGeom), so there is a large amount of axial force in beam that cannot be ignored.

    2) I exerted the displacement load on both columns.


    I would appreciate it, if someone could help me to do this.
    Regards,

  • Ali Akbar Shaikhzadeh added an answer in Tecplot:
    Which software should I use for making plots for research?

    Hi all,

    For creating my plots of research papers, I use MATLAB. However, MATLAB has some limitations on number of line styles (it has only four).

    I wanted to kindly ask you guys to share your experience on which software is best to be used for making 2D plots of research papers, MATLAB, Excel, Tecplot?

    Thanks

    Ali Akbar Shaikhzadeh · Ferdowsi University Of Mashhad

    During the last year, from start of this question, I have tried many software for my research plots. Each has some good and weak points. However, based on my experience OriginPro is the best among the others. It has excellent capabilities to create any kind of plots. It can also import data from Matlab directly.

  • Maureen F Legge added an answer in Motivation:
    Are there any known studies on the impact of testing mode (paper/computers/mobiles) on student motivation?

    Any known studies on the impact of testing mode (paper/computers/mobiles) on student motivation?

    Maureen F Legge · University of Auckland

    I think Mark's comment about assessment by stealth is an interesting one. I think learning by doing and then being required to explain, what you have learned by doing is a great way of assessment. It is a 'test' of ingenuity and understanding as opposed to memory and recall, or even just hit and hope. I am a strong advocate for experiential learning and from that drawing 'assessment' for the students or allowing them to respond to their learning through writing about it... not to answer my specific questions but to unpack what they have learned. Hope this makes sense!