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- 6How many can we days store 20 micrometer slices on adhesive slides?
I have cut 20 micrometer slices using Cryotstat and put them on adhesive slides, Can anyone tell me for how many days i can stores this slides and at what temperature, in case i want to use it for some more immunofluorescence staining to be done later.
You can store 20 micron thick sections at -20 degree for 1 year for Immunocytochemistry ( in box). I Didn't try for longerFollowing
- 4If the percent of third monomer in terpolymer is very low is it possible using copolymerization kinetics?
Recation follows copolymer kinetic or no?
If the percent of third monomer in terpolymer is really very low - the problem to detect its presence will entirely depend on sensitivity of the method applied for detection. Like in FTIR spectroscopy - very often it is impossible to detect the presence of co-monomer if its concentration is less than 1-2%Following
- 1How can I measure the number of photons absorbed in photocatalysis?
How Can I measure the Absorbed number /moles of Photons / Radiation?
First you must validate your experimental geometry with a chemical actinometer.
If you know the extinction coefficient of your compound and the radiant flux from actinometry...the number of the absorbed photon can be calculatedFollowing
- NewWhat is the best way to overcome flatfoot deformity in Cerebral palsy child ( GMFCS I &II)?
Does Kinesio taping have any beneficial effect to overcome flat foot deformity?Following
- 3Are VTEs risk factors in ICU?
Recently this article was published in Critical Care:
It included risk factors for VTE specific for ICU patients (such as sedation and vasopressors). table 3
My question, if we are to include these risk factors in a VTE risk assessment score, how many points each should be accounted for?
Requires RCT to analyse the impact of various variables contributing to VTE and add RAS for each.Following
- 4Where can we find a good description of male genitalia of species of Hymenoptera Diapriidae subfamily Diapriinae?
We want to make photo's of some male genitalia of Hymenoptera Diapriidae.
I already have Snodgrass (1941) and the publications with genitalia of members of the subfamilies Belytinae (in Nixon 1957) and Ambositrinae (in Naumann 1982).
Hi Theo, I just looked to see if Anthony Daglio was on Researchgate.net, and found he isn't. If you join Academia.edu, you will see masses of articles on wasps that he has uploaded to be shared. Various countries, various kinds. He has collected and is continuing to collect a treasure trove of articles/books on hymenoptera from all over.
I joined Researchgate to be able to see an article I was interested in, and Academia to get one pdf file. I think these communities do an invaluable service for researchers in many surprising areas. And, just as in Researchgate, people are not snooty about postings from people without much name for themself in a field.
- 4Do we have any comprehensive review of the data regarding prevention of fungal foot infections (Athlete's foot)?
Hi! Do we have any comprehensive review of the data regarding prevention/prophylaxis of fungal foot infections (Athlete's foot) in different categories of patients, including antifungal agents, certain devices, etc.? Thanks.
- 6How can I calculate the value of distortion for PO4 tetrahedra for single crystal data?
It is really distorted, two types of bond lenth and angles are different.
you can also calculate the "volume-deviation" from idealized tetrahedra as suggested by Balic-Žunic and Makovicky.
 T. Balic-Zunic, E. Makovicky, Acta Crystallogr. Sect. B 1996, 52, 78–81.
 E. Makovicky, T. Balic-Zunic, Acta Crystallogr. Sect. B 1998, 54, 766–773.
H. A. Höppe applied this Methode for different Phosphates, we have explained this methode for example here (Z. Anorg. Allg. Chem. 2010, 636, 1106–1110)
"The deviation of the PO4 tetrahedra from ideal symmetry
was calculated applying the method of all ligands enclosing
spheres on experimental data. In this method not only distance
variations inside a polyhedron are considered but also angle
deviations caused by larger distortions. In a first step the optimum
centroid of the four surrounding oxygen atoms, i.e. the
phosphorus atom, is determined by a least-squares refinement
 and gives a medium centroid-ligand distance rcentr. Subsequently,
the volume of an ideal tetrahedron given by Equation
1a is calculated using rcentr. Finally, this volume is compared
with the volume of the experimentally obtained body built up
by four vertices . This volume can be calculated using
8r 3centr/(9 √3) (1a)
where S is the base area built from three vertices and h is
the height of the body obtained by the distance of the fourth
vertex from the base"Following
- 2Is it safe to draw blood through a short peripheral catheter?
Research to substantiate blood draws through a short peripheral catheter
Obtaining blood from short peripheral catheter has lot of disadvantages as described by Barbara Hewer. It would not be recommended atleast by the current standards.
Results in failure of the catheter requiring new insertion,
Reliability of the sample obtained ?
Risk of infection to the patient both systemic and local with contamination are some of the factors to be consideredFollowing
- 5Has anybody got some experience with manipulating direct positive and negative cross-group contact via an online-survey?
Has anybody got some experience with manipulating direct positive and negative cross-group contact via an online-survey? Thanks in advance!
I am not a "tech freak" but is it any chance for some kind of video conference for example using skype?
- 2Is it mandatory to perform right heart catheterization to confirm pulmonary hypertension to get a publication through a peer review?
Although I exclude patients with pulmonary hypertension due to left heart disease. What echocardiographic parameters would allow to confirm pulmonary hypertension apart from mPAP (calculated from sPAP)?
Thanks in advance!
Thank you very much! It is truly a very helpful advice.
Best regards, VladFollowing
- 16Which Likert scale should I prefer that of 1-4, 1-5 or 1-7?
I'm making a questionnaire for my students about my new teaching style. My question is about the best Lickert scale that I can use in order to they evaluate some statements: 1-4, 1-5 o 1-7? I have read about this, but there are advantages and disadvantages in each of them. Can anyone help me out?
Thank you in advance.
Most studies concerning the use of either 5 or 7-item scales do not necessarily confer any absolute recommendation favoring one approach over the other. However, a study showed a slight support to use 7-point scale among respondents with more cognitive ability like student respondents, and use 5-point scale when respondents are general public (look at the reference 1 below). However, the authors seemed somewhat indifferent in using 5-point or 7-point scale at the end of their article. Another study conducted in European perspective utterly recommended 5-point scale to provide better quality of data compared to 7 or 11-point scale (look at the reference 2 below). Interestingly, the study falls in line with recommendations as outlined in reference 1, that 5-point scales be used when respondents are general public.
- Weijters, B., Cabooter, E., & Schillewaert, N. (2010). The effect of rating scale format on response styles: The number of response categories and response category labels. International Journal of Research in Marketing, 27(3), 236–247. http://doi.org/10.1016/j.ijresmar.2010.02.004
- Revilla, M. a., Saris, W. E., & Krosnick, J. a. (2013). Choosing the Number of Categories in Agree-Disagree Scales. Sociological Methods & Research, 43(1), 73–97. http://doi.org/10.1177/0049124113509605
Hope they help you in deciding on this issue.Following
- 3How do I calculate areas in continuous raster of species distribution models (5 classes)?
I just generated continuous models of species distribution models using MaxEnt. Now I would like to calculate the areas (in ha) occupied by each suitability class (5 classes: very low (0 - 0.2), low (0.2 - 0.4), medium (0.4 - 0.6), high (0.6 - 0.8) and very high (0.8 - 1)). Can anyone suggest methods or softwares for that?
ArcGIS can do this. Extract by Attributes allows you to select your ranges and this produces a separate raster for each range. Convert your floating raster (created from the asc file) to integer raster using Raster calculator (you might have to first multiply up your values by 10000 to eliminate the decimal). You can now use zonal statistics as table to calculate the area of your rasters. Make sure your data is in a suitable projection such as UTM rather than WGS84.Following
- 99+Is it time we shift emphasis from technological solutions to climate change & focus on the 'Human Dimension'?
Isn't the obvious solution and the elephant-in-the-room 'BETTER HUMAN BEINGS'? Shouldn't the focus be on better human beings rather than better technology? Why is it that everyone wants to develop better technology rather than focus on better humanity? Because no one has the answers and no one wants to change themselves? In environmental degradation, is it not obvious that nature can heal itself, if only left alone, and it is we humans who need regulation? Many natural parks managers do just that; seal off the area from human interference to let nature heal and recover. It is classified as 'Strict Nature Reserve"by IUCN. Complacency and inaction are not advocated here, as many have misunderstood, but the shifting of focus from technology to the human being. As technology is no match for human greed, isn't introspection & restraining ourselves more relevant than developing more technology, which caused the mess in the first place, by making it easy for a few to consume more? Since technology is only a short term quick fix which fails after a short time, isn't the real problem our addiction to material consumption & our lack of understanding about human nature? Isn't developing more technology sustaining the addiction instead of correcting it, leading to more complex problems later on, needing more complex technological quick fixes like higher drug dosages, more ground troops & equipment, (along with their debilitating side effects) in the future? Isn't this the vicious addiction circle we are trapped in? As researchers, do we merely buy more time with technology OR go to the very root of the problem, the human being?
A lot of hue and cry is made about climate change and the environment in general. Public and private money is poured into research to study its effects on the environment, sustainability etc. Should we study nature or ourselves?
" Our studies must begin with our selves and not with the heavens. "-Ouspensky
Human activities have been found to have a direct correlation to climate change and its impact on the environment(I=P x A x T, the Ehrlich and Holdren equation), in spite of what some complacent sections say to protect their own self interests.
We hardly know about Human nature. We can scarcely predict human behavior. We need to find out why we think like we do and why we do what we do and why, in spite of all knowledge and wisdom, consume more than what we need, in the form of addictions to consumption and imbalance not only ourselves but also the family, society and environment around us..
Humanity is directly responsible for all the unnatural imbalances occurring on the planet. Yet we refuse to take responsibility and instead focus on climate change, or fool the public exchequer with a 'breakthrough in renewable energy just around the corner'. We scarcely know what drives human beings. If we had known, all the imbalances around us would have had solutions by now, given the amount of money plowed into finding such solutions. Are we blindly groping in the dark of climate change because we don't know the answers to our own nature?
Is it not high time we focus on what makes us human, correct our consumptive behavior and leave nature to take care of climate change? Why focus effort on 'externals' when the problem is 'internal'- 'me'?
Aren't we addicts denying our addiction and blaming everything else but ourselves?
" We are what we Think.
All that we are arises with our thoughts.
With our thoughts, we make the world." - Buddha
IMHO, We don't need to save the World. It is enough if we save ourselves from ourselves. The need of the hour is not vain glorious interventions, but self-restraint and self-correction!
The Mind is the Final frontier.
Once again, we've come full circle to the issue of population! The root driver of population growth is natural selection, which I commented on seemingly countless times. Here is where we can do some constructive work, along with better understanding the consequences of our actions. Its Thanksgiving day in the US, and we are preparing for the celebration!Following
- 3Does anyone faces problems in expressing soluble Fab from pCOMB3XSS vector in TOP10 bacteria?
Does anyone faces problems in expressing soluble Fab from pCOMB3XSS vector in TOP10 bacteria? if yes how do you solved..our anyone have experience in expressing in other amber codon supressor bacteria strain to express soluble Fab? Thank you
I got lost midway. You extract phage DNA after selection by panning, and then transform it into TOP10. At this stage you get individual colonies, but none of them expresses a soluble Fab. How was it that you determined that "after transform the TOP10 bacteria with these pools DNAs I successfully have expression of Fab"?
By the way, how are you measuring expression? Coomassie-stained SDS-PAGE, Western blot, ELISA of rupture supernatants...?Following
- NewWhen soil solution is concentrated four times, K-Ca activity ratio in solution will change?
As we know that activity ratio is K /Ca or K /Ca+Mg is influences soil solution.Following
- 1Why does the shift shock phenomenon occur in lower gears as compared to higher gears in automatic transmission engine?
Shift shock is the jolt felt when transmission changes gear. This jerk or shock feeling is observed in lower gear shift (i.e 1-2 or 2-3) and not in higher gears shift (i.e 5-6 or 6-7). What is the reason for such behavior.
It is the rotational moment of inertia which scales up with i²Following
- 2SSPA or TWTA, which one should I consider to design High power amplifier in c-band satellite communication?
SSPA is linear and TWTA is more efficient. How are linearity and efficiency of high power amplifier related? Where should I concentrate more; on linearity or efficiency?
Thank you Md. Sakir Hossain.
I appreciate your participation. If i am not wrong, Higher the efficiency more the transmission power required. Sometimes if some amount of transmission don't take place then it creates heat in a concentrated area of the transceiver. If this happens then it is challenging to keep PA cool. this is my concernFollowing
- 2Can anyone help me to identify ectoparasites samples that I found on a long eared hedgehog?
Can anyone help me to identify ectoparasites samples that I found on a long eared hedgehog?
thank you for your helping. best regardsFollowing
- 1Is there anyone who used Kerns Secure Attachment test before?
I am struggled with the data entering. If anyone used this scale for attachment, could you please inform me about that? Should I just use cut-off points (45) as determine secure/insecure attachment or is there any other classification system for this measurement?
and anyone knows how to enter data into the SPSS for Secure attachment?
Thanks in advance
I think you can use cut-off score and you can do this by using one sample t test as following:
analyze + compare means + one sample t test
then enter the dependent variable and the test valueFollowing
- NewHow i can be sure of the facultative endosymbionts in aphids are alive by measuring by PCR ?
If i have a wide range of facultative endosymbionts in Acyrthosiphon pisum, how i can measure or determine if these are alive or death by PCR.Following
- 9Are there any papers for 'school as a service'?
I am interesting in this field and i would like to read some papers (if there are any).
The attached links will be of use to youFollowing
- NewSocrates:Regarding sound and color, would you not say that both are? Theaetetus: Sure, also that each is different and identical to itself. How not?
The 'principle of identity' is defined also as a philosophical principle that generally indicates the equality of an object relative to itself.
In relation to other objects, identity is everything that makes an entity definable and recognizable, because it has a set of qualities or characteristics that make it what it is and, thereby, set it apart from all other entities.
The concept of identity thus connects with Aristotle and the concept of substance, a principle that allows things to remain identical to themselves in time and which causes two things to be identical between them because they have the same substance. From Aristotle's view it will result that in criticism to the concept of substance in modern philosophy, as in David Hume, it will be necessary to challenge the character of identity.
In philosophy starting from the eighteenth century with Christian Wolff, Alexander Gottlieb Baumgarten and Immanuel Kant it spreads the expression of "the principle of identity and non-contradiction" that was historically preceded by the "judgment of identity" which has a different meaning. Judgment, in fact, that is to report, asserting or denying a predicate to a subject, affirms that "one thing, if it is, can not be other than that which is. It denies the possibility of any judgment in which the predicate is different from the subject, that is not identical judgment. "If "Socrates is" the identical judgment will say that "Socrates is Socrates"; you will never make a judgment that says "Socrates is not Socrates."
Then when it comes to the principle of identity (A is A) that would re-awaken the useless judgment of identity if you did not link to the Aristotelian "principle of contradiction" whereby "or A is B, or A is not B" where the Eleatic contrast between being and not being is outweighed by the fact that to say "is not B" is not to deny the being of A, but simply that A (keeps being in that) is different from all the other things other than it . So the subject is at the same time itself (identity) and other (otherness) by anything else.
The "principle of identity and contradiction" is developed within Scholastica that puts together the Aristotelian principle of contradiction with the assertion, not Aristotelian of identity of subject and predicate
"A is A, A is not A"
in an attempt to reaffirm the Eleatic immutability of being in the identity of subject and predicate: The principle of identity of indiscernible.
Distinct from ‘principium identitatis et contradictionis’ is instead the special principium identitatis indiscernibilium mentioned in the age of ancient philosophy, medieval and modern art for which it is impossible that there are individual objects perfectly identical (in which case it would not constitute a plurality of reality but of a single reality) then you can only affirm the identity of an object with itself not we being able to grasp the distinction between objects absolutely identical, indiscernible among themselves.
Kant criticized supporting this principle as a multiplicity of actually completely identical to each other, however, retained their multiplicity which could be grasped by observing how these were at a distinct and different mode in space.
The philosophy of identity belongs to those philosophical systems which, starting from the distinction between two heterogeneous realities then considers the same in relation to a higher reality to which the first two belong. An example of these doctrines is Schelling's philosophy where the two spheres of the real and the ideal imply each other since they are the two poles of which one is the power of the other and both are expressions of the Absolute to which two moments are essential: the identity and the difference, or in other words, units and opposition. The absolute is that which is defined as the identity of "identity and difference."
The introduction of the difference makes multiplicity possible. The explication of the absolute in the infinite multiplicity of the universe is really necessary because the time difference is as essential as that of identity. This activity occurs in a polarity of opposing forces, one positive and one negative (+ / -), but the positive (attraction) configures it as A, the negative (repulsion) configures it as multiple and polarized, such that each pole is in turn the union of a '+' and '-'. The One is found in many, and many are endless facets of the One.
The identity in the strict and absolute sense will be, according to Leibniz, when of two things, everything that can be predicated truly of the one can really be predicated also of the other, that is, when the name of the one is interchangeable with that of the other in all the judgments in which this appears, without altering the value of truth.
Logical thinking has taken the contemporary definition of Leibniz, but ruled that it is permissible to speak of identity in an absolute sense, because the identity is always relative to a determined linguistic set, that is, to a certain attribution of meaning to names. The principle of identity puts it this way: everything that is, is or also everything is identical to itself. Hired as a fundamental principle of logical truths with Kant, was a very important part in the post-Kantian philosophy, to Hegel, who took the paradoxical character, so it is effectively denied to every proposition in which subject and predicate are different terms, that is, from most cases of which it makes use (of the form "A is B"). For Hegel, however, the principle of identity must become the principle "of identity and non-identity," "preserving" and "overcoming" at the same time the contradiction.Following
- NewAny lab for molecular identification of carnivore scats in world top 200 Universities???
Any lab for molecular identification of carnivore scats in world top 200 Universities???Following
- 2Does anyone know any papers or useful information on cognitive bias that relates to age?
I am currently undertaking my dissertation, using Harding et al’s generic approach in a judgement bias test using dogs. The study uses a novel, portable apparatus to train an operant, auditory discrimination task and subsequently test cognitive bias. Dogs are required to perform an auditory discrimination task, and their latency to respond will be recorded to reveal their expectations and therefore their judgement bias. The study will collect data on judgement bias in a range of ages in dogs, and will investigate levels of optimism and pessimism and explore factors that may affect the expression of judgement bias.
If anyone has any relevant information i'd appreciate it :-)
I have used the judgment bias task to assess the affective state in pigs. I have recently published a paper assessing the consistency of the cognitive bias over time. My results show low consistency between the same test over time and one of the hypothesis is that the age plays an important role. The name of the paper is : Cognitive Bias in Pigs: Individual Classification and Consistency Over Time. If you don't have access to the paper, let me know and I will send you a pdf copy.
- NewWhich approach to reconstruct the human-induced and natural historical events in a remnant wetlands?
As wildlife manager (and applied ecologist) of a protected area, I'm reconstructing the history of natural and anthropogenic events that have featuring a wetland remnant ecosystem embedded in a fragmented and urbanized landscape in the last thirty years. I have observed how the management of these systems should take particular account both deterministic/periodical events and rare, stochastic, unpredictable events ('black swan' events).
I would like to know if this arena of historical applied ecology (Swetnam et al.) has already been investigated by some researcher in wetland management and which approach have been used.Following
- 3Can I use goat serum instead of mouse serum as a blocking agent for flow citometry?
I'm doing intracellular immunohistochemistry for flow cytometry. My conjugated antibody was raised in mice, so I know that for blocking when preparing my flow cytometry samples, I should be using normal mouse serum. I understand that this serum will compete with my antibody at non-specific sites.
The sample to be stained is of human origin.
My question is, can I use goat serum instead of mouse serum? Won't goat serum also bind at these non-specific sites?
Mouth mouse serum would be the most appropriate in this case. Several controls are indicated for immunohistchemical procedures such as absorption of first antibody with the specific antigen and cross reactivity with others antigens. Consultation with the manufactures of the antibodies and or the kit you are using in my practice has usually provided the best advice. They usually have technical service to answer specific question.Following
- 5What can explain the variability in peak sizes of my standard solutions using HPLC?
When I am making up a master mix including 3,4 dihydroxybenzoic acid, dopamine and serotonin (100ng/ml in 0.15M PCA) and I split this over multiple autosampler tubes, the peak sizes of these standards in each tube differ quite considerably when I run them on the HPLC. When I run these standards repeatedly, the general trend is a reduction in peak size for all standards (although this does not happen every time), but the relative differences in peak sizes between the tubes is maintained.
Standards are prepared on ice and tubes are kept in a refridgerated autosampler (4C) until they are run.
Has anyone encountered a similar problem? Or does anyone know what could possibly cause this or have any suggestions what to try next?
Thank you very much!
Thanks for the suggestions!
I will try stabilise my standards and will let you know how it goes.Following
- 4How can I do the interaction effect between D and R on the Dependent Variable A?
I test a structure equation model of five variables 2 of them independent and 2 moderate and one is dependent.
the relationships in mplus are
D by d1 d2;
R by r1 r2;
A by a1 - a4;
M by m1 m2;
S by s1 - s3;
A on R D S
S on M R
R on M
the question is:
How can I do the interaction effect between D and R on the Dependent Variable A??
I think you can use Two Way Anova for this purposeFollowing
- 4What type of sensors can be used to analyze a magnetic flux density on a surface of a sample?
I am planing to analyze magnetic flux density on a surface of samples. The material will be low alloyed steel with a ferritic microstructure. The range of the magneitc flux density will be lower than 0.2 mT. So far, I used Hall effect sensors. What other sensors can you suggest?
geometry includes lateral dimensions to be analyzed -- a magnetic force microscope is essentially an atomic force microscope with a suitably prepared magnetic structure on the cantilever. So, the lateral extent of the area which can be scanned is restricted and might not suit your needs.
And sorry, I am the wrong person to ask for expertise in MFM --- I have only heard and discussed on talks from which I have mostly remembered that this is not a push-the-button-and-get-a-valid-result type of method. I have never actually done this myself.
Depending on the required resolution (you were talking about microstructure, so you might not need to reach the nanometer scale), magneto-optical microscopy might be an alternative (depending on what exactly you need to measure). There is also a contact foil enhanced method people have used to image superconducting microstructures and their response to magnetic fields (if I remember right). Check the publications of Joachim Albrecht, there should be a PRL from maybe 10 years ago which made it to the title page.
And I'll browse to your page -- but not today, sorry.Following