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Answer added to:1 Gateway vectorYou can check this paper, the basic principles of Gateway cloning are quite well explained there, and it also explains some of the applications for pl... [more]
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Answer added to:19 Transformation efficacyYou want to keep the volume of DNA less than 10% of the volume of the cells. Preferentially as little as possible.
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Answer added to:7 I am cloning a 4400 base pair gene using pET-28a vector. After many attempts I am not successful. Please suggest how can I get a full gene.Dear Deepan Shah, Thanking you very much for the information .I will be doing the changes as per your suggestions .Will also be changing molar ratio.A... [more]
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Answer added to:8 How to identify false positive clones in transformation?Thank you all so much. Yes the problem was with the antibiotic concentration. I made new agar plates and the culture media with antibiotic and I am ge... [more]
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Answer added to:1 pU3Z-MCS-NOS plasmid mapIs this used for sugarcane ?? chk these out http://patentimages.storage.googleapis.com/EP2348116A2/imgf0021.png http://www.docstoc.com/docs/77996571... [more]
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Answer added to:1 Nucletoide bases in mRNA.At the 5' end, bases are removed and altered, I seriously doubt that bases are added. Think about how DNA is replicated.
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Question:Open How to transfer a resistance gene from Triticum durum (Tetraploid) to Triticum aestivum (hexaploid)?Rust resistance breeding
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Answer added to:12 Cotton Transformation...Dear Asif for my knowledge it will be better i.e. protocol for Coker 312 & 310.......So please can you send me that on santosh_ippar@rediffmail.com.
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Question:Open Is there a possibility for local rhizobium species from Malaysia to be used to transfer foreign genes into plants?A few locally isolated rhizobium were used mainly in improving crop yield. I'm inspired by the fact rhizobiums could transfer genes as efficient as ag... [more]
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Answer added to:17 Does anyone know if Kanamycin (aminoglycoside bacteriocidal antibiotic) actually promotes plant growth?Dear Piet van der Linde, i have the same guess before when i observed this occurance,i am glad that you have carried out great work about it. TQ
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Answer added to:5 How can I over express a protein in a wild type strain?I can not suggest for any suitable vector for bacillus, but you may find lots of literature on successful vector for overexpression in Bacillus. I jus... [more]
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Answer added to:5 Any thoughts on "best practices" for Saccharomyces paradoxus transformation?Hello, it has been a few months. Could you tell me if your transformation work? I am trying to integrate 10 kb yeast expression plasmid 7kb + my GOI 3... [more]
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Answer added to:1 Have you tried in planta transformation of maize?What about this method ? Have you got satisfying result ? I used shoot tip of maize as the transformation receptor mediated by agro ,but the efficien... [more]
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Answer added to:3 Hi. What is the best method or protocol for transformation of Bacillus subtilis?Your welcome, I like helping people.
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Question:Open Hai, I need the diarmed Agrobacterium strain C58C1 carrying the plasmid pRiA4 of Agrobacterium rhizogenes for the transformation of medicinal plants....Please help me
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Question:Open Does anyone have a transformation protocol for Cryptococcus curvatus?I am working with wild type C curvatus and am not aware of an existing transformation protocol.
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Question:Open In search of a lab to perform genetic transformation of tomato for wilt resistanceI have a grant to develop protocol and stably transform our local tomato varieties for disease resistance. I am in search of a suitable laboratory whe... [more]
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Answer added to:4 Topo cloningCheck via through invitrogen process... Catalytic process should be in ur favor,,because the kinetic complexity increases in in ampicillin....or use a... [more]
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Answer added to:2 Fungal transformation - protoplast preparation.Dear Dr. Cheng: Thank you very much for the link to the protocol for Protoplast preparation from Aspergillus. Will this work for A. niger or A. oryz... [more]
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Question:Open Transformation problem...unable to express pseudomonas in dh5alpha host of e.coliRespected Sir, Do u have any idea about the ideal vector for Pseudomonas species expression? Can i use DH5alpha vector of E.Coli to express Pseudomona... [more]
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Answer added to:5 Agrobacterium transformationHarpreet, Congrats.
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Answer added to:1 efficiency of transformationIt's easy. Using untransformed cells, take a sample of your competent cells (I suggest 10uL) and perform 10-fold dilutions and plate each dilution on... [more]
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Answer added to:7 so confused.....:-(one more thing...prepare competent cells of E.coli for transformation.
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Answer added to:2 L. lactis transformationThanks, i have used the protocol mentioned in NIZO sites, i wil try the protocol you have mentioned, hope for best
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Answer added to:11 plant tissue cultureI did some work on maize tissue culture some years back, but can assist if you interact at stefedha@gmail.com
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Answer added to:5 Himay i know about your details
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Answer added to:4 Transformationyes, I performed. competent cells, my vector and my construct. Thanks,
About Transformation (Genetics)
In molecular biology transformation is the genetic alteration of a cell resulting from the direct uptake, incorporation and expression of exogenous genetic material (exogenous DNA) from its surroundings and taken up through the cell membrane(s). Transformation occurs naturally in some species of bacteria, but it can also be effected by artificial means in other cells.
