Tissue engineering is the use of a combination of cells, engineering and materials methods, and suitable biochemical and physio-chemical factors to improve or replace biological functions. While it was once categorized as a sub-field of bio materials, having grown in scope and importance it can be considered as a field in its own right.

• Enrico Lucarelli

  Ovine Demineralized Bone Matrix

  I am biologist involved in the research in Tissue Engineering and Regenerative Medicine in an Orthopaedic Hospital in Italy. Bone harvested from human donors (cadavers) is often used to reconstructI am biologist involved in the research in Tissue Engineering and Regenerative Medicine in an Orthopaedic Hospital in Italy. Bone harvested from human donors (cadavers) is often used to reconstruct bone. Donors’ bone is frequently used after having been ground and demineralised. I am currently looking for ovine demineralised bone matrix, I would appreciate it if somebody could suggest where I can find it.

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• Karthick Govindaraj

  Can anyone advise on the most suitable substrate for growing corneal fibroblast from the following substrate options?

  PEO-PPO copolymer (Pluronic F127) coated TCP (Tissue Culture Plastic) Polycaprolactone – Aligned Nanofibers on coverslip Polycaprolactone - Film on coverslip Which is the best ?

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    Boris V Stanzel replied

    you might find that your cells will grow faster on PCL NF, in any event, PCL is a good material to try. would be curious to hear what you find. we work mostly with epithelial cells.

    21 days ago
• Basel Kotsa

  Can anyone recommend where to find reliable information about biomaterials usable for bone regeneration?

  Biomaterials

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    Hans Leemhuis replied

    As Anabela already posted, I would suggest to look for a good review. There are plenty of Biomaterials for Bone Reg. and which to choose depends on the application you have in mind. Below someAs Anabela already posted, I would suggest to look for a good review. There are plenty of Biomaterials for Bone Reg. and which to choose depends on the application you have in mind. Below some reviews that coudl be useful: Bone regeneration: current concepts and future directions (2011) Rozalia Dimitriou1,2†, Elena Jones3†, Dennis McGonagle3† and Peter V Giannoudis1,2*† http://www.biomedcentral.com/1741-7015/9/66 Bone grafting, orthopaedic biomaterials, and the clinical need for bone engineering. (2010) Brydone AS, Meek D, Maclaine S. PMID: 21287823 Bone tissue substitutes and replacements. (2011) Theler JM. PMID: 21677587 Enjoy !

    26 days ago
• Philippe Bouaziz

  Cutting 3D scaffold

  Does someone know a strategy to cut ECM base scaffold for confocal analysis, I tried cryosectioning but cryogenize ECM material are just to difficult to cut properly (include in OCB glue)?

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    Philippe Bouaziz replied

    Thank you for all your responses, I try myself all the methods you suggest but I find that just cutting with a scissor the scaffold between two layers of paraffine work if you searching to do aThank you for all your responses, I try myself all the methods you suggest but I find that just cutting with a scissor the scaffold between two layers of paraffine work if you searching to do a transversial view. Cheers. Philippe.

    Apr 26, 2012
• Xiao Li

  A problem about collagen hydrogel with primary hepatocytes in it

  Recently I'm confronting a hugely disturbing problem about collagen hydrogel. I prepared SD rat's tail collagen type I by myself basically according to a paper on "Nature Protocols". And I usedRecently I'm confronting a hugely disturbing problem about collagen hydrogel. I prepared SD rat's tail collagen type I by myself basically according to a paper on "Nature Protocols". And I used 10xDMEM (10% in the fina mixedl solution), Collagen in HAc (50%), fresh SD rat's hepatocytes solution in WE medium (40%) and a little NaOH to mix neutral solution, as to form collagen hydrogel with hepatocytes. I placed this solution in incubator (37 °C and 5% CO2) for about half an hour, and this solution turned to be very yellow, which indicated that it was acidic. I used tested these samples and found pH below 6. And thus, collagen wasn't able to gel, for the lack of neutral environment. For the purpose to fix this problem, I added HEPES into the mixed solution and carefully modified the pH to be 7.4 before putting it into incubator. But it still became yellow after a longer time, and failed to gel. This problem is very very frustrating. Has anyone met this similar situation? Or would you give me some possible explanation to this? I'll appreciate your help very much! FYI, in the mixed neutral solution, the collagen concentration was 6 mg/ml, and the cell density was approximately 8E6/ml.

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    Enrico Marani replied

    See Mouveroux et al Dev Brain Res 122 (2000) 59-66

    Apr 26, 2012
• Xiao Li

  Anyone has experience of injectable hydrogels in tissue engineering or drug delivery?

  Can collagen be gelled in situ? In other words, is collagen hydrogel an alternative as injectable hydrogels? Besides, does anyone know how to reduce the gelling time of collagen hydrogels, maybeCan collagen be gelled in situ? In other words, is collagen hydrogel an alternative as injectable hydrogels? Besides, does anyone know how to reduce the gelling time of collagen hydrogels, maybe through some kinds of modification?

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    Enrico Marani replied

    See Mouveroux et al Dev Brain Res 122 (2000) 59-66.

    Apr 26, 2012
• Philippe Bouaziz

  Does anyone have experience on spraying stem cells ?

  Stem cell spray?

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    Sridhar Rao Kunchala replied

    yes, Fiona Wood is one of the pioneers of stem-cell spray for treatment of burns. Many technologies similar to this are now available in many clinics.

    Apr 20, 2012
• James Kobler

  FDA - implantable hydrogels

  Does anyone have experience with FDA IDE applications for implantable hydrogels? I am interested to hear of your experiences.

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    Hardik Baviskar replied

    Mr. Joseph; I agrees with you; as per teh earlier comments; it seems that Mr. James is formaulating a new product; then as per the defination; iet will go under the new drug class; the sameMr. Joseph; I agrees with you; as per teh earlier comments; it seems that Mr. James is formaulating a new product; then as per the defination; iet will go under the new drug class; the same would require the clincial trials; pre clinical data if available on the same product can be adopted by the regualtory bodies; depending on the quality and the relevence of the data that would be products; I can sugges to add those relevent literatures ;etc. so that it will strenthen the application and can be exemted.

    Apr 20, 2012
• Karthick Govindaraj

  The incorporation of Electro spun polymeric scaffolds in bone engineering.

  Can anyone explain how the topography plays an important role in bone engineering?

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    Hans Leemhuis replied

    I think for bone engineering, as for almost all concepts in regenerative medicine, the scaffold structure (i.e. topography, pore size, pore structure) is essential and maybe one of the most importantI think for bone engineering, as for almost all concepts in regenerative medicine, the scaffold structure (i.e. topography, pore size, pore structure) is essential and maybe one of the most important factors. Sure, scaffold material, mechanical and chemical properties are also important. But scaffold structure plays a very important role in how the cells seed and remodel the scaffold. In bone, there is no clear opinion, which factor is most dominant. But from my point of view, scaffold structure is critical and in most cases underestimated.

    Apr 18, 2012
• Ashwini Kumar

  What clinical progress has been made in tissue engineering except that of dental implants and bone graft? Any other organ been successfully used?

  I wanted to know if any artificial organ has been transplanted successfully yet?

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    Hazem Orabi replied

    urinary bladder-urethra

    Apr 12, 2012
• Divya Rathore

  Can anyone tell me upto what extent tissue engineering is safe?

  As tissue eng is not a easy deal . I just wish to know that what are all would be the harmful effects of tissue engineering?

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    Divya Rathore replied

    No problem. Its ok sir :-)

    Apr 12, 2012
• Jothi Muniyandi

  What is the role of retenoic acid in stem cell research?

  describe the Mechanism of the retenoic acid in stem cell research?

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    Jothi Muniyandi replied

    Thanks you..........................

    Apr 8, 2012
• Yang Liu

  How to test the strength and elasticity of a piece of cell sheet.

  I want to test the strength and elasticity of a piece of cell sheet about 2mm^2 size. Is there any advise, any method and any instruments? The cell sheet is too small and too fragile to pick up.

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    Mythili Jagannath replied

    Thanks will look up

    Apr 4, 2012
• Divya Rathore

  How single gene defect causes brain tumor?

  

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    Divya Rathore replied

    Thank you sir ........ Can we treat it by using gene therapy or nt?

    Mar 7, 2012
• Ramesh Bulbule

  tissue engineering

  I have been looking for courses in Tissue Engineering with application for Dentistry. Does any one know any place where these courses may be available. I would only like modular or distance educationI have been looking for courses in Tissue Engineering with application for Dentistry. Does any one know any place where these courses may be available. I would only like modular or distance education courses or part time courses. I cannot leave my present job and take up full time studies.

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    Ramesh Bulbule replied

    Thanks James. But its irrelevant to my querry. Thanks again.

    Feb 24, 2012