@Vladimir Teif thank you for the paper.

The paper suggests that the nucleosome positioning at the 5'end is ATP driven despite obstructions. What the paper doesn't suggest is why..

When we consider why is the process ATP driven we can understand that the 5'end has a progressive transcription activity which has mechanisms to keep it nucleosome free. When the nucleosome formation tries to occur in these regions it has to overcome against the repelling mechanisms, that's where our ATP comes into picture.

What I don't understand is why should there be a nucleosome formation occuring in this region. I had always understood the nucleosome formation to be a DNA sequence and histone compatibility bonding. Why should there be an excessive energy spent on nucleosome formation if the DNA sequence doesn't approve it. Epigenetic mechanisms to hypermethylate may prove to decrease transcription activity by clouding the TSS and prompter region so that the Transcriptional machinery cannot access it also become histone repelant.

But is the epigenetic process also involved in nucleosome packing at 5'end I believed it was the opposite.
Not only that the area around the TSS and the 5'end has a predominantly high transcription activity irrespective of the exon constitution. We must not forget about the pervasiveness of the 5'end being one of the factors for the TSS's high transcription activity.

So why does nucleosome packing occur at the 5' end?

Second question under consideration is that 5' nucleosome packing operates faster than replication-dependent nucleosome assembly why ? Can anyone clarify that at any point of time a span of DNA may be subjected to replication, transcription at the same time. If so then what decides the nuclesome packing. When does the 5' nucleosome packing occur and when not.

thnx got it

yeah paper no accessable

Hi this really is a great topic, I don't have access to the paper but can you give a gist of the points presented in the paper? I'm concerned more with the elements present at the vicinity of the TSS which disturbs the chromatin remodelling. CpG islands .. also interested hw the nucleosome organization can give access to transcription of may regions which were not found to be transcribing before.

interesting