Plant Biotechnology

• Karen Pachchigar

  Identification of anti-cancerous gene in a plant

  I'm currently working with an unexploited medicinal plant from India that has proved to have anti-cancerous effects in chinese medicine. I want to identify the anti-cancerous gene from that plant.I'm currently working with an unexploited medicinal plant from India that has proved to have anti-cancerous effects in chinese medicine. I want to identify the anti-cancerous gene from that plant. Can anyone give me some advice on which systematics should follow in order to identify this potential gene?

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  • 
    Baleshwar Meena replied

    Dear Sir, We are working in our lab on systematics and diversity of plants, using molecular markers we try to resolve the taxonomic and systemacs problems among various taxa. Your problems is like aDear Sir, We are working in our lab on systematics and diversity of plants, using molecular markers we try to resolve the taxonomic and systemacs problems among various taxa. Your problems is like a gene expression problem. So you must approach a molecular biologist working on gene expression. Baleshwar

    7 hours ago
• Cristine Menezes

  How can I study floral polymorphism into the same specie?

  I tought in to study the genes expression, but there aren't nothing printed about which responsable genes or pathways metabolic for polymorphism of the colour and size flowers for this specie. SomeI tought in to study the genes expression, but there aren't nothing printed about which responsable genes or pathways metabolic for polymorphism of the colour and size flowers for this specie. Some suggestion?

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    Karen Pachchigar replied

    go 2 KEGG pathway nd chk pathway of flavonoids as in most case flower pigmentation comes wid phenolocs expression nd mostly favonoids nd anthocyanin so jst chk it nd write clearrly for which type ofgo 2 KEGG pathway nd chk pathway of flavonoids as in most case flower pigmentation comes wid phenolocs expression nd mostly favonoids nd anthocyanin so jst chk it nd write clearrly for which type of problem u want to study gene expression...best f luk

    8 hours ago
• Morsli Abdelkader

  RAPD or RFLP of some Algerian populations of Datura

  I work on the genetic diversity of Datura in Algeria. I lack references and the technical means, and need help to finalize work on the morphological diversity. Any recommendations? Thank you

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    Cristine Menezes replied

    Hi Morsli, both RAPD and ISSR primers are random. I think good options: Operon primers to RAPD and ISSR primers from the University of British Columbia, Biotechnology Laboratory (UBC, Vancouver,Hi Morsli, both RAPD and ISSR primers are random. I think good options: Operon primers to RAPD and ISSR primers from the University of British Columbia, Biotechnology Laboratory (UBC, Vancouver, Canada). Other possibility which can better reproducibility to RAPD is to use high annealing temperature (~ 48°C; HAT-RAPD) or convert markers RAPD and ISSR in SCAR markers.

    22 hours ago
• Pradeep Shrestha

  I am about to perform a novel plant tissue culture. What are the important factors for optimizing the culture ?

  I have to do the suspension culture too

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    Pradeep Shrestha replied

    thanks for the comment, these are all set up but i am not getting what are the factors that i need to consider for getting the callus, usually hormones are done but why only the MS media , are therethanks for the comment, these are all set up but i am not getting what are the factors that i need to consider for getting the callus, usually hormones are done but why only the MS media , are there any supplements to be added. I am to perform the tissue culture of new plant that is indigenous to Nepal. Thanks

    1 day ago
• Reena Sharma

  Can anyone tell me the method to measure total phosphorus in dry plant tissue?

  I have got a digestion method but it is not very clear. Could you suggest or reference any protocols?

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  • 
    Reena Sharma replied

    Thanks...Tesha.

    4 days ago
• Leonardo Guzzetti

  What kind of material should I extract for a Sorghum germination library?

  I need to build a library for one-hybrid assays with Sorghum. It has to be a germination library and my main question is: What kind of samples should I collect to extract the needed RNA?

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  • 
    Leonardo Guzzetti replied

    I just realized that we are trying to construct a library with the wider range of conditions. We have 35 DAP embryos and I might get some other samples to buiild the library. We don't want toI just realized that we are trying to construct a library with the wider range of conditions. We have 35 DAP embryos and I might get some other samples to buiild the library. We don't want to prove any hypothesis, we're just trying to know wich FTs interact with a cloned promotor. Later on we'll build another library or some other libraries for different comparisons between different DAPs and conditions as you people suggested. THANKS A LOT for the answers.

    9 days ago
• Antonino Testa

  18th of May 2012, first international "Fascination of Plants Day" http://www.plantday12.eu/
• Darshi Patni

  Can any one tell me which is better for in-vitro micropropagation*-- liquid culture media/ solid culture media/ semisolid culture media?

  I also want to know the reason for it and there comparision

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  • 
    Dong-mei Kong replied

    It's hard to say which is better or absolutely best. It depends on your cultured material and purpose. Solid culture is most common used culture method. But for cell ture, maybe, liquid culture isIt's hard to say which is better or absolutely best. It depends on your cultured material and purpose. Solid culture is most common used culture method. But for cell ture, maybe, liquid culture is better. If you want to carry out somatic embryogenesis, liquied culture coule also be sonsidered.

    11 days ago
• Meenu Ghai

  Calculate the size of a restricted fragment of LAMP product

  To ensure that the LAMP reaction amplified the target DNA, we normally restrict the product and run it on gel. How can I calculate the size of a restricted fragment of LAMP product?
• Reby Skaria

  Can anyone recommend a method for checking anti oxidant activity in plants?

  Can anyone recommend a method for checking anti oxidant activity in plants, particularly banana? Is the crude extract required.

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  • 
    Chander Malik replied

    The best is to estimate total phenols; polyphenol oxidase; SOD activities. There are both enzymatic ( catalaser, SOD, peroxidases) and non-enzymatic antioxidants ( ascorbic acid, glutathione,The best is to estimate total phenols; polyphenol oxidase; SOD activities. There are both enzymatic ( catalaser, SOD, peroxidases) and non-enzymatic antioxidants ( ascorbic acid, glutathione, tocopherol, carotenoids, etc)

    14 days ago
• Reby Skaria

  Can anyone suggest the best way of cryopreservation in banana?

  How we can cryopreserve the musa germplasm?
• Muthukumar Chelliah

  Can anyone tell me the method for quantification of total terpenoid content in the plants?

  Can anyone tell me the method for quantification of total terpenoid content in the plants?
• Faheem Khan

  Wheat transformation

  I know the best possible explant for transformation in wheat is immature embryos but the problem related to it is, it's too laborious and the time period to isolate it is too limited. Can anyone helpI know the best possible explant for transformation in wheat is immature embryos but the problem related to it is, it's too laborious and the time period to isolate it is too limited. Can anyone help me know what can I do if I missed that crucial time for immature embryo isolation. Which explant I can use for transformation except immature or mature embryos in wheat?

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  • 
    Hatem AbdEl-Ghany replied

    I did this work many times, it is very intersting and enjoyable but it need more time and Lab possibilities

    25 days ago
• Faheem Khan

  Determination of trehalose in wheat with HPLC

  I wish to know which is the best available way to determine trehalose concentration in wheat plant?

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  • 
    Prof. Zoran Vujcic replied

    or http://www.megazyme.com/downloads/en/data/K-TREH.pdf

    25 days ago
• Stephen Chakravarthi

  Which tissue culture method is best for anther culture in pearl millet?

  Also need male sterility mechanism in bajra