Discussion of the plant sciences

• Zeinab Abdo

  Determination of tree age

  Any one work in determination of trees age by using core cutting or know of any protocols or research in this topic?

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    V.S. Ramachandran replied

    By carbon dating is one method:By counting growth rings is another method.

    2 hours ago
• Rita Zomuanpuii

  Morphological identification of Orchids to species

  I have few orchids of my own which are indigenous to my state (Mizoram) as a hobby. I don't have any idea on their morphological identification to species. I have photographs of the flowers. Can II have few orchids of my own which are indigenous to my state (Mizoram) as a hobby. I don't have any idea on their morphological identification to species. I have photographs of the flowers. Can I have some help? PS: I have uploaded thirteen (13) photos if you scroll down the comments.

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    Rita Zomuanpuii replied

    Thank you all, You have been very helpful

    4 days ago
• John Adams

  Plants containing alkaloid-terpenoid complex: is there any histochemical method to detect this complex and prove its complexity?

  I am working on plant histochemical studies. I detected Alkaloid and terpenoid by using Dragendroff's and vanilin- perchloric acid tests respectively. I want to prove it in a single test to give theI am working on plant histochemical studies. I detected Alkaloid and terpenoid by using Dragendroff's and vanilin- perchloric acid tests respectively. I want to prove it in a single test to give the positive result to complex form of Alkaloid -terpenoid using an histochemical method

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    Suresh Bansal replied

    Ok, what is the difference between the crude oil and bark oil as both are biotic in origin and linked with living organism.

    12 days ago
• Sajna Cholakulangara

  Which buffer and pH suits best for protein isolation from rice varieties?

  To extract total protein form rice variety matta triveni

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    Suresh Bansal replied

    If we have a sample of bark oil from any tree. Can be identify in labs that this oil is from any living thing without knowing it prior?

    13 days ago
• Celeste Varela

  Can anybody help me with a technique for measuring abscicid acid and its metabolites of shurbs?

  the aim of my study is to measure ABA and its metabolites of shurbs from Patagonia Argentina (the shurbs is Larrea divaricata). My problem is that the leaves of this shrubs have a lot of resin formedthe aim of my study is to measure ABA and its metabolites of shurbs from Patagonia Argentina (the shurbs is Larrea divaricata). My problem is that the leaves of this shrubs have a lot of resin formed mainly for NDGA (Nordihydroguaiaretic acid) and this compound form a complex with ABA, which does not allow the extraccion of the hormone for subsequent quantification in LC-MS/MS. Before the step of extracting of the hormone for its cuantification, I tried washing the leaves to remove the resin and the NDGA, but it turned out the washing solutions used also extracted the hormone. The solutions tested were NaOH and Chloroform at different concentrations. Could someone advise me another solution to wash the leaves that do not extract the hormone? Celeste Varela · 32 minutes ago · Delete this question · Edit this question
• Anesia Tampus

  What makes mangosteen fruit famous?

  Zango drink has been proven to be very effective herbal medicine against high blood pressure, heart failure, cholesterol level, and help almost in all diseases. It is 97% mangosteen in its contentZango drink has been proven to be very effective herbal medicine against high blood pressure, heart failure, cholesterol level, and help almost in all diseases. It is 97% mangosteen in its content and remaining are preservatives, organic sugar and etc.

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    Wim Goeteyn replied

    do you mean Xango instead of Zango?

    26 days ago
• Natallia Laspina

  Genome size of Polygonum species.

  I need help about the genome size of Polygonum species. I haven't found any paper on this. Any recommendations?

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    Natallia Laspina replied

    thaks! I found this site. May be helpfull for others. RBG Kew DNA C-values

    27 days ago
• Maryia Ivanova

  Is it possible to ex vitro conditions of growing to equate to greenhouses conditions? And why?

  Is it possible to ex vitro conditions of growing to equate to greenhouses conditions? And why?

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    Maryia Ivanova replied

    Jose Mejia · Light for Hope Foundation × Maryia, Do you call Tissue Culture micropropagation? yes. my last publication: http://phytomorphology.org/PDF/MP2/02053054.pdf

    Apr 27, 2012
• Anju Babu

  Is browning of the callus a problem?

  I studied browning of the madia and encountered a problem in tissue culturing. This may occur as a result of nutrient depletion, contamination etc.. After subculturing of callus and a period ofI studied browning of the madia and encountered a problem in tissue culturing. This may occur as a result of nutrient depletion, contamination etc.. After subculturing of callus and a period of incubation how should we know the callus is responding positively? Is browning of the callus a problem?

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    Pallawi Upadhyaya replied

    Yes, browning of your cultured tissue is a problem and certainly hinders with its growth performance. therefore it is advisable to get rid of it ASAP. Also, a growing callus is visually different andYes, browning of your cultured tissue is a problem and certainly hinders with its growth performance. therefore it is advisable to get rid of it ASAP. Also, a growing callus is visually different and shows soft, friable cell masses or shoots/organs (whatever the case may be).

    Apr 25, 2012
• Rudolf Schlechter

  Is it fine to just cut leaf material and store it in 2.0 mL tubes at -80 ºC?

  I need to store leaf material for DNA extraction and I wonder if I can just cut frozen leaves (with liquid nitrogen) in small pieces and then store it at -80ºC in 2.0 mL tubes? There should be noI need to store leaf material for DNA extraction and I wonder if I can just cut frozen leaves (with liquid nitrogen) in small pieces and then store it at -80ºC in 2.0 mL tubes? There should be no problem with endonucleases, right? I accept gladly some tips :)

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    Pallawi Upadhyaya replied

    Of course it is alright if you have snap frozen it and your leaves are never thawed before coming in contact with the extraction buffer.

    Apr 25, 2012
• Anju Babu

  Why are phenolic compounds are produced during plant tissue culturing?

  Production of phenolic compounds in the medium is a disturbance or contamination during plant tissue culture, but how are these compounds produced? Is it due to any nutrient scarcity in the medium?

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    Wasim Rashid Kakroo replied

    Dear, it is not silver chloride...it is silver nitrate.....the concentration is needed to be optimised as per needs.....it is usually between 2-5 mg/l.......

    Apr 23, 2012
• Delphine Chinchilla

  Protein extraction from N. benthamiana

  I am searching for a good and quick protocol of protein extraction from N. benthamiana to avoid protein smir: any suggestion? I thought of adding DTT and PVPP but is that really essential? any otherI am searching for a good and quick protocol of protein extraction from N. benthamiana to avoid protein smir: any suggestion? I thought of adding DTT and PVPP but is that really essential? any other trick? I use benthi as transient expression system.

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    Ahmed Z. Abdel Azeiz replied

    Protease inhibitor cocktail is very essential to be added during protein extraction by any method to prevet protein hydrolysis.

    Apr 18, 2012
• Raksha Bawankar

  Is their any Institute or any other source who can supply Arabidopsis plants, or advise on appropriate growing conditions?

  We have grown Arabidopsis plants in our laboratory within the required growth conditions. But, maybe due to the lack of proper growth conditions, they are not showing good growth. I need to performWe have grown Arabidopsis plants in our laboratory within the required growth conditions. But, maybe due to the lack of proper growth conditions, they are not showing good growth. I need to perform phytochemical as well as gene level studies on Arabidopsis thaliana and need to know if their is any source available to supply Arabidopsis thaliana.

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    Raksha Bawankar replied

    Thanks a lot every body for your useful suggetions...realy it was helpful for me.. Best Regards

    Apr 20, 2012

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    Chengara Sreemadhavan replied

    You may want to pay special attention to the leaf architecture in your studies. I worked on the family leaf architecture of Acanthaceae and related families and found the variations in the leafYou may want to pay special attention to the leaf architecture in your studies. I worked on the family leaf architecture of Acanthaceae and related families and found the variations in the leaf architecture very useful. Hickey Yale) and coworkers have produced an ecellent manual of leaf architecture that can be used. I feel strongly that a key based entirely on leaf architecture can be created for the species of most genera. I did this for Andrographis (Acanthaceae) and Chamaesyce (Euphorbiaceae) of South Florida. MANUAL OF LEAF ARCHITECTURE BETH ELLIS, DOUGLAS C. DALY, LEO J. HICKEY, KIRK R. JOHNSON, JOHN D. MITCHELL, PETER WILF, AND SCOTT L. WING “Since the earliest days of scientific botany, subtle variation in leaf form has been both informative and confusing for specialists and nonspecial- ists alike. The Manual of Leaf Architecture places comparative studies of living and fossil leaves on a new and more secure footing. It will be indispensable for anyone using the leaves of living or fossil plants in their morpho-developmental, systematic, or ecological research.” —Professor Sir Peter Crane FRS, University of Chicago 216 pages | $29.95 hyflex | A Comstock Book 330 halftones, 33 line drawings

    Apr 19, 2012
• Kees Bakker

  Have anyone experience with using 6-Bap to get more buds on each spike of phalaenopsis?

  Phalaenopsis, 6-bap

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    Ashok Ahuja replied

    Spray BAP using 500ppm on plants in Green House to get more buds

    Apr 10, 2012