Mohammad Yasin added an answer:Does anyone know the half-life of leupeptin?
I would like to test leupeptin by injection intraperitoneally into rats who have received a brain injury, but need to know the half-life in order to determine the best dosing time-points. Any insight would be much appreciated!
Usually it is 7 min.Following
Jens Bak Sommer added an answer:Does anyone know how to deal with reduced motivation in the accelerating rotarod test?
We are currently using the accelerating rotarod test to assess vestibulomotor functioning following experimental brain injury in rats. In this regard, we have encountered that it can be very difficult to motivate animals to run on the rotarod: Some animals jump off the rod after a few seconds, whereas others seem to develop a strategy where they deliberately ‘fall’ off the rod in a more or less convincing manner after a relatively short time interval (e.g. 30 seconds). In both cases the performance/score does not appear to reflect the true vestibulomotor functioning of animals, and the performance of some animals vary significantly from trial to trial.
In order to establish a baseline performance level we have been giving animals 10 trials (5 days of 2 trials) before injury. Subsequently animals have been tested on the rotarod for 7 days (2 trials per day) post-injury. We are using a commercially available rotarod from PanLab/Harvard Apparatus (fall height 21 cm, rod diameter 60 mm, acceleration 4-40 rpm over 2 minutes).
If anyone has had similar experiences and has any ideas or advice on how to increase motivation in the rotarod test, it will be highly appreciated.
Thank you for your reply and data, Hanna. I will let you know how everything turned out after my next round of experiments.
Thanks again :0)
Raoul Christian Sutter added an answer:Can anyone recommend a validated observer-rated pain scale for nonverbal adult patients with a stroke/brain injury?
The setting is a neuro rehabilitation unit, patients aged 18 years and up. Thank you for your help.
Dear Krystal, perhaps this link and the attached paper will be of some help...
Grace Elizabeth Pluhar added an answer:Can anyone recommend a protocol for GLIOMA animal modelling ?
Has anyone tried GLIOMA animal modelling ? It will be really helpful if anyone could kindly suggest me a good protocol to follow.
Regardless of the induced model you choose, remember that there are limits to their translational relevance. Pet dogs, specifically brachycephalic breeds, develop high grade glioma spontaneously, and are a highly relevant model.Following
Maurizio Iocco added an answer:Have you ever seen in peripheral traumatic nerve injury a neurological repair without functional recovery?
I'm sure that in a few cases we have seen patient with persistent loss of function after a peripheral traumatic nerve lesion, but their conduction was normal.
Have you too?
Do you know why?
Yes I understand. It's true. But this is known for the central mechanism of recovery. but why in peripheral? I'm starting with a study about this problem.Following
Nima Derakhshan added an answer:Can glibenclamide be used as an adunctive therapy for cerebral edema in traumatic brain injury?The use of glibenclamide to control plasma glucose after TBI had no significant effect on patient outcome at discharge, but it could reduce the LOS-NICU (p<0.05). Glibenclamide also had no apparent effect on the presence of PSH in TBI patients with type 2 diabetes mellitus.Dear Kenneth
Corticosteroids are contraindicated in TBI as they resulted in worsening of outcomes in recent studies
It s a guideline according to evidence based dataFollowing
Yanick Simon asked a question:What is the reason for projectile vomiting and nausea due to elevated intracranial pressure?Elevated intracranial pressure is often associated with vomiting. I would like to know how the pressure triggers the vomiting.Following
John Daugherty added an answer:Management of post head-injury Bruxism.Patients with severe head injury esp , diffuse axonal injury may have associated bruxism which, to my experience, lasts for 10 -14 days. I have been studying these patients in recent years with trials of pramipexol and benzodiazapines. I have come across the botox inj studies as well. I wish to have suggestions from colleagues.Non-invasive treatment modalities with symptom appropriate ancillary care can be very efficacious. With any head injury the trigeminal system is ramped up and it interrupts afferent signals improperly, even normally innocuous signals. Well developed night guards can reduce the excessive motor intensity of V3 and possible on the over all upper quarter pain.
Svensson, P; Jadidi, F; Baad-Hansen, L; Sessle, B.J. Relationships between crainofacial pain and bruxism. Journal of Oral Rehabilitation, 07/2008, Volume 35, Issue 7, pp. 524 - 547.
Lobbezoo, F. Naeij, M. Bruxism is mainly regulated centrally, not peripherally. J Oral Rehabil, 2001. 28(12): p. 1085-91.Following
Mustafa Volkan YAPRAKCI added an answer:Hi everybody!This is a newly started group for everybody interested in neurotraumatology.
Closed account added an answer:Physician ScientistHallo I am new memeber of the groupWelcome!Following