The identification and study of the totality of the neurons and nervous tissue in an organism

• Smitta Shukla

  Fetal emotions

  Do fetal or neonatal emotion exist in the same or similar manner to that of adults?

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  • 
    Syed Abedin replied

    I have similar experience of dog bite and subsequent fear of dogs.

    4 days ago
• Kobra Tahmasebi

  What is relationship between zinc and memory?

  Topic in this subject

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    Farzin Safaiee replied

    The subsynaptic distribution of reactive zinc suggests three possible modes whereby synaptic zinc could affect cognitive processes: (i) tonic binding of zinc to extracellular protein domains withThe subsynaptic distribution of reactive zinc suggests three possible modes whereby synaptic zinc could affect cognitive processes: (i) tonic binding of zinc to extracellular protein domains with nanomolar affinity; (ii) phasic modulation of surface proteins following exocytosis of vesicular zinc; (iii) tonic or phasic modulation of cytosolic proteins in pre- or post-synaptic compartments.[This is part of a paper with this title:Modulation of Neuronal Signal Transduction and Memory Formation by Synaptic Zinc- PMCID: PMC3211062]

    5 days ago
• Üner Tan

  Is the superior cerebellar pedunculus crossed in apes, too?

  The superior cerebellar pedunculus is crossed in humans. I'd like to konw if this crossing also exists in other primates, too.
• Kristiina Pulli

  What are the main general differences in mouse and human brain tissue composition?

  We are routinely preparing mouse brain homogenites, from whole hemispheres and also from microdissected tissues like striatum, hippocampus etc and detecting Huntigtin protein from those samples byWe are routinely preparing mouse brain homogenites, from whole hemispheres and also from microdissected tissues like striatum, hippocampus etc and detecting Huntigtin protein from those samples by MSD and western blot. We use ceramic beads and FastPrep24 homogenizer, and our lysis buffer contains 20mM tris, pH 7.5, 150 mM NaCl, 1mM EDTA and 1mM EGTA supplemented with protease and phosphatase inhibitors and PMSF). And ususally it works very well. Recently we tested our procedure on human brain samples (a small piece of cortex and striatum), but we were not able to efficiently extract Huntigtin in the lysate, instead most of it remained in the unlysed pellet (confirmed by western blot). Maybe this could be due to some general differences in the composition of mouse and human brain tissue? Does anybody have experience/knowledge what those differences might be? Or in general experience on working with human brain lysates? This might help us to optimize our procedure. Any ideas are appreciated, thank you in advance!

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    Daniel Clark replied

    Kristiina, you might find some helpful information from this similar question which was answered just last week:Kristiina, you might find some helpful information from this similar question which was answered just last week: http://www.researchgate.net/topic/Neuroscience/post/I_need_to_know_the_neuroanatomy_of_embryonic_mouse_brain_cortex_ie_mainly_which_neurons_form_the_cortex_n_hippocampus_extend_their_axons_where Hopefully that can help you out!

    12 days ago
• Yingpei Wong

  I would like to know the growth condition for SH-SY5Y cell lines in the medium and without differentiation, as well as subculturing.

  Apparently, I am growing the SH-SY5Y cells. But it grows very slowly and doesn't reach the expected confluency after a few days. I couldnt do the subculture because the cell confluency was too low.Apparently, I am growing the SH-SY5Y cells. But it grows very slowly and doesn't reach the expected confluency after a few days. I couldnt do the subculture because the cell confluency was too low. What is the best way to do it?

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    Vicente Lieberknecht replied

    Actually, as soon cells detach from de flask or plate we add 1:1 medium with FCS 10% to neutralize trypsin. Than, we aspirate this mixture (cells, trypsin and medium FCS 10%) centrifugue, ressupendActually, as soon cells detach from de flask or plate we add 1:1 medium with FCS 10% to neutralize trypsin. Than, we aspirate this mixture (cells, trypsin and medium FCS 10%) centrifugue, ressupend the cells with medium and dillute the cells as much we want to make them grow in experimental determined time. In my experience, a million cells will grow in four or five days.

    Apr 14, 2012
• Trisha Theile

  Tissue Stains

  I am looking to find any information on Tissue Stains; I am staining slices of the brain of Huntington's Disease mice. Currently I am using TTC but would like to incorporate other stains into myI am looking to find any information on Tissue Stains; I am staining slices of the brain of Huntington's Disease mice. Currently I am using TTC but would like to incorporate other stains into my study as well. Does anyone have any suggestions and protocols for the usage? Any help would be greatly appreciated! Thank you, Trisha

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