- Lei Shang added an answer:How can I identify the cell with the staining of Annexin V (-)/PI (+) using flow cytometry?Annexin V (+)/PI (+) represent necrosis and later apoptosis, Annexin V (-)/PI (-) represent normal, Annexin V (+)/PI (-) represent early apoptosis. So what about Annexin V (-)/PI (+), please help me and attach your related references.Maybe someone give me the answer about primary necrosis, but how to understand this kind of cell death, what the relationship between primary necrosis and necrosis??Following
- Demba Sarr added an answer:Does anyone know the best way to induce Necroptosis in cells? TNF, TRAIL...?I would like to induce necroptosis in trophoblast cell line.Thank you Scott. I will keep you posted and sorry for the delay.Following
- Bohyun Choi asked a question:How can I identify necroptosis cells?Is there a marker for necroptosis cells?
Is it able to distinguish necroptosis cells from apototic cells?Following
- Lei Shang added an answer:How to knockdown the expression of RIP3?I cannot find a inhibitor of RIP3 to make the deletion. Can somebody give me some information to attenuate protein level?Thanks Linkermann!! I agree with your comment.Following
- Dominique Vanhecke added an answer:Is there a natural situation (in vivo) where intact endosomes could be released from cells?What happens to endosomes following cell death (apoptosis, necrosis etc).Thank you very much for your input.
But I was wondering whether in addition to these microvesicles there can also be intact organelles, in this case intact endosomes be released under some circumstances. A big difference is that in contrast to microvesicles the endosomes would be so-called "inside-out" with respect to the orientation of membrane bound proteins.Following