For researchers, professors, graduate students, post-docs.

• Annapoorani C L

  Cytotoxicity studies

  Hi all! I am doing my project regarding Magnetic nanoparticles combating tumor cells... How can I proceed the Cytotoxic studies for the same.?!?!

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  • 
    Audra A Kerwin replied

    You could also assay for apoptotic markers such as caspase 8

    9 days ago
• Nikhil Chauhan

  Which type of nanoparticles should we use which are bio-compatible and targeted to organs, while also easily eliminated from body?

  Nanoparticles in drug delivery

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  • 
    Audra A Kerwin replied

    PLGA is FDA approved very biocompatible. Silica dioxide mesoporous nano particles are looking very promising as well.

    9 days ago
• Adam Hughes

  Is there a gold nanoparticle equivalent to the standard biacore SM5 dextran-matrix coated gold surfaces?

  I have had success binding proteins to gold nanoparticles with n-alkanethiol monolayers; however, almost every subsequent protein introduced into the system will non-specifically bind to this layer. I have had success binding proteins to gold nanoparticles with n-alkanethiol monolayers; however, almost every subsequent protein introduced into the system will non-specifically bind to this layer. BiaCore chips use a custom carboxymethylated dextranmatrix to, among other things, limit non-specific binding. I know that PEG and OEG layers on gold nanoparticles can also limit this non-specific binding; however, I have not found a golden recipe to effectively build a surface on gold np's with similar properties to BiaCore chips. Does anyone know of such a coating that would make gold np's: 1. Resistant to non-specific interactions. 2. Have a covalently reactive headgroup for targeting a receptor protein (for example an NHS-Ester to covalently bind proteins displaying primary amines). I affix the nanoparticles to a surface so aggregation is not an issue.

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  • 
    Stephan Barcikowski replied

    you may checxk also the company website www.particular.eu, they specialized on nano-gold with high purity and bioconjugation

    17 days ago
• Marwa Matboly

  I need a method to quantify the number of amino groups on amino functionalized Magnetic nanoparticles

  We are synthesizing amino functionalized magnet particles to conjugate with a thiol modified oilgonucleotide probe. We need to know the number of amino groups on the iron in to order to adjust theWe are synthesizing amino functionalized magnet particles to conjugate with a thiol modified oilgonucleotide probe. We need to know the number of amino groups on the iron in to order to adjust the amount of thiol modifed oligonucleotide to optimize cojugation through the cross linker .

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  • 
    marwa matboly replied

    thank y v much Bhart

    19 days ago

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  • 
    ABDUL RAZAK MOHAMED SIKKANDER replied

    Dear Amro A.HUSSEIN, Please refer the following paper : J Biomed Mater Res B Appl Biomater. 2009 Oct;91(1):213-20. In vitro/in vivo biocompatibility and mechanical properties of bioactive glassDear Amro A.HUSSEIN, Please refer the following paper : J Biomed Mater Res B Appl Biomater. 2009 Oct;91(1):213-20. In vitro/in vivo biocompatibility and mechanical properties of bioactive glass nanofiber and poly(epsilon-caprolactone) composite materials. Jo JH, Lee EJ, Shin DS, Kim HE, Kim HW, Koh YH, Jang JH. Source Department of Materials Science and Engineering, Seoul National University, Seoul, Korea. Abstract In this study, a poly(epsilon-caprolactone) (PCL)/bioactive glass (BG) nanocomposite was fabricated using BG nanofibers (BGNFs) and compared with an established composite fabricated using microscale BG particles. The BGNFs were generated using sol-gel precursors via the electrospinning process, chopped into short fibers and then incorporated into the PCL organic matrix by dissolving them in a tetrahydrofuran solvent. The biological and mechanical properties of the PCL/BGNF composites were evaluated and compared with those of PCL/BG powder (BGP). Because the PCL/BG composite containing 20 wt % BG showed the highest level of alkaline phosphatase (ALP) activity, all evaluations were performed at this concentration except for that of the ALP activity itself. In vitro cell tests using the MC3T3 cell line demonstrated the enhanced biocompatibility of the PCL/BGNF composite compared with the PCL/BGP composite. Furthermore, the PCL/BGNF composite showed a significantly higher level of bioactivity compared with the PCL/BGP composite. In addition, the results of the in vivo animal experiments using Sprague-Dawley albino rats revealed the good bone regeneration capability of the PCL/BGNF composite when implanted in a calvarial bone defect. In the result of the tensile test, the stiffness of the PCL/BG composite was further increased when the BGNFs were incorporated. These results indicate that the PCL/BGNF composite has greater bioactivity and mechanical stability when compared with the PCL/BG composite and great potential as a bone regenerative material. (c) 2009 Wiley Periodicals, Inc. PMID: 19422050 [PubMed - indexed for MEDLINE]

    Apr 24, 2012
• PRITAM KUMAR Dikshit

  Which nanoparticles show good activity against microbes?

  Which nanoparticles show good activity against microbes?

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  • 
    Stephan Barcikowski replied

    In case of inorganic nanoparticles like Ag, Cu, Zn, independent of the material, the ion release is important. Do you want to apply the nanoparticles as colloid or embed them? In the first case, theIn case of inorganic nanoparticles like Ag, Cu, Zn, independent of the material, the ion release is important. Do you want to apply the nanoparticles as colloid or embed them? In the first case, the medium affects dissolution. In the second case, ion release may follow first or second order kinetics (Ag or Cu), and combination of materials can allow to tune and sustain the ion release. Further information on ion release and therapeutic window please find here: Hahn, A. ; Guenter, S. ; Wagener, P. ; BARCIKOWSKI, S.: Electrochemistry-controlled metal ion release from silicone elastomer nanocomposites through combination of different metal nanoparticles. In: Journal of Materials Chemistry 21 (2011), Nr. 28, S. 10287–10289 Hahn, A. ; Brandes, G. ; Wagener, P. ; BARCIKOWSKI, S.: Metal Ion Release Kinetics from Laser-generated Nanoparticle-Silicone Composites. In: Journal of Controlled Release 154 (2011), Nr. 2, S. 164–170 Hahn, A. ; Stoever, T. ; Paasche, G. ; Loebler, M. ; Sternberg, K. ; Rohm, H. ; BARCIKOWSKI, S.: Therapeutic window for bioactive nanocomposites fabricated by laser ablation in polymer-doped organic liquids. In: Advanced Engineering Materials 12 (2010), Nr. 5, S. B156–B162

    Apr 16, 2012
• Ravishankar Bhat

  What happens to gold nanoparticles after interaction with tumor cells?

  Looking for background on the mechanism of action of gold nanoparticles as anticancer agents.

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  • 
    Ravishankar Bhat replied

    Thank you sir... for useful information

    Mar 31, 2012
• Adam Hughes

  How should I bind antibodies to gold?

  Hi everyone, My question is in regard to antibody-gold nanoparticle conjugation. I have my gold nanoparticles affixed to a substrate so that they cannont move. Is it ok to simply incubate theHi everyone, My question is in regard to antibody-gold nanoparticle conjugation. I have my gold nanoparticles affixed to a substrate so that they cannont move. Is it ok to simply incubate the particles with an antibody and let physiological absorption be the primary mechanism for binding? Or is there are preferred method for binding antibodies to gold? I've seen both instances in the literature. I was able to bind the antibodies just with physiological absorption in one case, but the antigen was not detected well and I'm trying to troubleshoot.

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  • 
    Adam Hughes replied

    Hi Badri, My thoughts exactly. The group from which we've obtained the proteins is confident they work, but I'll have to see it with my own eyes on a western blot or Biacore until I'm comfortableHi Badri, My thoughts exactly. The group from which we've obtained the proteins is confident they work, but I'll have to see it with my own eyes on a western blot or Biacore until I'm comfortable saying for sure its working. As you said, it's strange to see no signal... Thanks for the input

    Mar 1, 2012

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  • 
    Dr. Amitava Banerjee replied

    Dear Amro, Please follow this link or find in Google, it may help you some way.. "Review: biofunctionalized quantum dots in biology and medicineDear Amro, Please follow this link or find in Google, it may help you some way.. "Review: biofunctionalized quantum dots in biology and medicine ...vt.academia.edu/.../Review_biofunctionalized_quantum_dots_in_biol.." Have a gteat day. Amitava

    Feb 16, 2012
• Muthu Vignesh

  Journal which accepts hypothetical paper on nanotechnology

  Hello, I had completed a hypothetical paper on nanotechnology for implantable biomedical devices. I had submitted in BME LETTERS of Springer 2 weeks back. However today i received a mail from theHello, I had completed a hypothetical paper on nanotechnology for implantable biomedical devices. I had submitted in BME LETTERS of Springer 2 weeks back. However today i received a mail from the editor stating that my paper was rejected. Can you please tell me any journals which accepts such hypothetical paper, any journal would okay for me as i am desperately looking for more publications.

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  • 
    Annapoorani C L replied

    Yeah sure sir..

    Feb 5, 2012
• Hojatollah Rezaei Nejad

  Can deionized water discharge charged macrosphere?

  I am doing an experiment with some charged particles, but despite the fact that they have number of negative carboxylic group (OH-), they don't move in electric field. I examined their solution inI am doing an experiment with some charged particles, but despite the fact that they have number of negative carboxylic group (OH-), they don't move in electric field. I examined their solution in deionized water. but they don't move???? Please kindly share what you think.

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  • 
    Hojatollah Rezaei Nejad replied

    I just tried solution with pH 2.2 and pH 11, but in fact the particle just move when the droplet is on bare electrode and there is a current. However, I need to move them toward each electrode whenI just tried solution with pH 2.2 and pH 11, but in fact the particle just move when the droplet is on bare electrode and there is a current. However, I need to move them toward each electrode when the electrodes are covered with Teflon and there is no current.

    Feb 1, 2012
• Dave Mitchel

  The researchers have registered concerns about the possible adverse effects of engineered nanomaterials in consumer products on human health and the environment.

  naturenanotech_optics_volkov_raman_1.pdf
• Hanh Tran

  DNA-Au NP conjugation

  Can anyone help me to resuspend red pellet of Au-NP after salt aging in Au NP and DNA conjugation? Thanks.

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  • 
    Eman Bader replied

    Salam give me ur email to send u the paper

    Oct 3, 2011
• Sudhanshu Pati

  Magnetic nanoparticles

  Could anyone explain the effect of magnetic nano particles on Biological objects. What are those molecules leading to the direct application of these particles?