Nano+Bio+Technology =Nanobiotechnology - 9 = Nano 10 Bio=biology =known organisms Technology =use of knowledge Nanobiotechnology = use of knowledge Nano particles and biology for known organisms and effects on cells

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• Pablo del Pino

  How does surface charge of nanomaterials influence on their toxicity?

  Is there a general consensus about the influence of charge (cationic, anionic, neutral) on the toxicity of engineered nanomaterials on culture cells and in vivo?

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    Hugh J Byrne replied

    Lots of work on PAMAM dendrimers - eg “In vitro mammalian cytotoxicological study of PAMAM dendrimers -towards quantitative structure activity relationships”, Sourav Prasanna Mukherjee, MariaLots of work on PAMAM dendrimers - eg “In vitro mammalian cytotoxicological study of PAMAM dendrimers -towards quantitative structure activity relationships”, Sourav Prasanna Mukherjee, Maria Davoren, Hugh J. Byrne, Toxicology in Vitro, 24,169-177 (2010) “Reactive Oxygen Species (ROS) induced cytokine production and cytotoxicity of PAMAM dendrimers in J774A.1 cells”, Pratap C. Naha, Maria Davoren, Fiona M. Lyng and Hugh J. Byrne, Toxicology and Applied Pharmacology, 246, 91-99 (2010) "Mechanistic studies of in vitro cytotoxicity of Poly(amidoamine) dendrimers in mammalian cells", Sourav Prasanna Mukherjee, Fiona M. Lyng, Amaya Garcia, Maria Davoren, Hugh J. Byrne Toxicology and Applied Pharmacology, 248, 259–268 (2010)

    3 days ago

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    Kiruba Daniel replied

    Encapsulation has to be done based on its application. if it is drug delivery, whether it is oral or IV, where it is supposed to reach , whether you are going to target using any antibody or peptideEncapsulation has to be done based on its application. if it is drug delivery, whether it is oral or IV, where it is supposed to reach , whether you are going to target using any antibody or peptide or any other means? all these plays a role in determining the encapsulating agent... You have to think and find which is the best encapsulation which can satisfy all ur needs. Wish you all success...

    3 days ago
• Amy Pieters

  Nanotechnology and teens. How can it interact.

  Dear collegues, I would like to know your thoughts about this ambiguous video http://bit.ly/tcmxq2 Thank you

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    Denis Zinchuk replied

    One of the most imnpressive russian movies on this topic

    18 days ago
• Patricia Khashayar

  Is developing a bionanosensor for osteoporosis feasible and possible? Anyone has experience in this regard?

  I am a bionanotechnology PhD student and am looking for a topic for my thesis. As I am working in the osteoporosis research center, I thought maybe it would be a good idea to choose a related topic.I am a bionanotechnology PhD student and am looking for a topic for my thesis. As I am working in the osteoporosis research center, I thought maybe it would be a good idea to choose a related topic. There are not many articles on a bionanosensor for osteoporosis so I wanted to know is there anything wrong with developing such a sensor?

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    Iris Miraballes replied

    Thanks Orestis

    24 days ago

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    Anand Lodha replied

    300 to 350 nm is the Range for Zinc Oxide nano particles.....

    25 days ago
• Swarup Roy

  How I can measure the concentration of Ag ions?

  I am trying to determine the concentration of silver ions. Is there a known estimation process?

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    Swarup Roy replied

    Thanks to all.

    29 days ago
• Sudheesh Kumar

  What is the tolerance limit of Gadolinium in humans?

  Gadolinium salt toxicity

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    Tejabhiram Yadavalli replied

    That would be a tough question Sudeesh. The parameters are so many. The size and shape for starters. then it would depend on the area being exposed to the metal. For a simple example we had done aThat would be a tough question Sudeesh. The parameters are so many. The size and shape for starters. then it would depend on the area being exposed to the metal. For a simple example we had done a DBT project on silver based membranes for water filtration. In that when we had to find out the toxicity of the silver nanoparticles, we had to specifically mention the size and shape of our material along with the area being exposed (in this case, the gastrointestinal tract), for us to say that silver nano is non toxic. Moreover the area being exposed is very essential because lungs are usually very very sensitive to foreign particles and sometimes even develop uncontrollable tumors (carcinoma) because of them. To be generic, if i'm not wrong, 30-300 ppm is allowable dosage for almost any material inside the body and will be slowly taken away by the RES. The material which are present in higher concentration and are not toxic are considered non-toxic materials.

    Apr 23, 2012
• Lalit Samant

  Is it possible to add nano particles to a plant-drug and do docking studies for a particular protein?

  I want to know whether we can do docking of nanoparticle + plant extract against a particular diseased protein?

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    Mohammad Reza Nejadmoghaddam replied

    Yes, exactly

    Apr 21, 2012
• Saurav Kumar

  effect of pH on the size of the particles during synthesis process?

  during any chemical coprecipitation or sol gel method what is the pH of solution is there any impact of that pH on size determination.

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    Mahmoud Reda replied

    The nano-size particles distinguish themselves from the bulk materials in the very high surface to volume ratio. The pH values at the surface may be different from the bulk value which is measuredThe nano-size particles distinguish themselves from the bulk materials in the very high surface to volume ratio. The pH values at the surface may be different from the bulk value which is measured experimentally.Low inter-facial pH means that the surface of the nano-partilces are positively charged.

    Apr 20, 2012
• Hanh Tran

  5 fold concentrated Au NP

  Hi everyone. I'am a biology student. Now, I 'm finding a way to conjugate DNA and Au-NP. I meet troubles with the term" 5 fold concentrated Au NP solution". Are there anyone can help me, plz?

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    Stephan Barcikowski replied

    this term is not useful, since there is no concentration standard. the closest may be an OD (optical density) of five. some suppliers give optical density values in their catalogue

    Apr 16, 2012
• Denis Zinchuk

  NT-MDT is happy to invite to the webinar “Tip-Enhanced Raman Scattering. Approaching 10 nm spatial resolution in Raman imaging” by Dr. Pavel Dorozhkin. Tip-Enhanced Raman Scattering (TERS) is theNT-MDT is happy to invite to the webinar “Tip-Enhanced Raman Scattering. Approaching 10 nm spatial resolution in Raman imaging” by Dr. Pavel Dorozhkin. Tip-Enhanced Raman Scattering (TERS) is the technique utilizing a special AFM probe (nano-antenna) to localize light at the nanometer scale area near the probe apex. When scanning the sample with respect to the probe, the obtained optical (Raman or fluorescence) maps have lateral resolution which is not limited by the light diffraction. A deep integration of AFM with confocal Raman microscopy is required for successful TERS experiment. In this webinar we will review the recent TERS results obtained by NT-MDT customers. Enhancement factors of a few orders of magnitude are observed using gold or silver TERS probes. 2D Raman maps with lateral resolution down to 10 nm are obtained for different types of samples: graphene, carbon nanotubes, organic molecules etc. Webinar speaker Dr. Pavel Dorozhkin, Head of Product Management and Application Division Date and time: Europe https://www2.gotomeeting.com/register/382823522 Tuesday, March 20, 2012 11:00 AM - 12:00 PM CET (2:00 PM - 3:00 PM Moscow daylight time) USA https://www2.gotomeeting.com/register/749748714 Wednesday, March 21, 2012 10:00 AM - 11:00 AM PDT (9:00 PM - 10:00 PM Moscow daylight time)

  http://www.ntmdt.com/news/view/the-webinar-ters-approaching-10-nm-spatial-resolution-in-raman-imaging

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    Dave Mitchel replied

    Thanks for records of the webinar, Denis! Next time I'll be online for sure.

    Apr 8, 2012
• Carlin geor Malar

  Will the type of nanoparticle affect its application?

  I am synthesizing magnetic nanoparticles (iron oxide) and immobilizing pectinase in it. If i use it for bioscoring, will the process depend only on the enzyme or the nanoparticle also? And since theyI am synthesizing magnetic nanoparticles (iron oxide) and immobilizing pectinase in it. If i use it for bioscoring, will the process depend only on the enzyme or the nanoparticle also? And since they are nan particles, will the separation process be tedious?

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    Gowhar Naikoo replied

    Type of nanoparticle changes properties also. As microporous materials(< 2nm in diameter) have different properties than macroporous materials(> 50nm diameter).

    Apr 3, 2012
• Tejabhiram Yadavalli

  Can someone suggest the easiest and best way to PEGylate my particles?

  I have synthesized metal ferrite nanoparticles in the size range of 40-50nm (ferromagnetic). I would like to now coat them with Polyethylene Glycol. Could someone suggest the easiest and best way toI have synthesized metal ferrite nanoparticles in the size range of 40-50nm (ferromagnetic). I would like to now coat them with Polyethylene Glycol. Could someone suggest the easiest and best way to PEGylate my particles such that the composite size doesn't cross 100nm.

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    Tom Morgan replied

    You may be able to work with what you have already. If your particles are already dispersed in water it likely means they are stabilized by some organic acid (EDTA, citrate, etc.). If this is trueYou may be able to work with what you have already. If your particles are already dispersed in water it likely means they are stabilized by some organic acid (EDTA, citrate, etc.). If this is true you can use the EDC/NHS chemistry that Ana Bohorquez (see above) has suggested. In this case you would want to attach mPEG-amine to your surface. I've done this successfully in the past using citrate stabilized calcium phosphate particles. Also, if you are concerned about increasing your particle size, there are a number of shorter chain mPEG-amines out there.

    Mar 28, 2012
• Suresh Kumar

  Has any study been done on the effect of nanogold/organic complexes on peptides/aminoacids, in vivo/in vitro for neurodegenerative diseases like AD?

  There is a lot of discussion in ancient treatment systems, as well as current traditional medicine practices that report positive effects of gold bhasma [essentially gold processed in herbal extractsThere is a lot of discussion in ancient treatment systems, as well as current traditional medicine practices that report positive effects of gold bhasma [essentially gold processed in herbal extracts and calcined, as particulate materials ofnano/micro gold organic complexes]. I have also been associated in an advisory capacity in this respect to certain lab programs that report effect of nanogold/gold complexes on globulin/peptides with corelative results, under investigation, with some promise in respect of neuroregenration.