Please give me protocol for the study of heavy metal tolerance and antibiotic resistance property in bacteria isolated from tannery effluent.

The method apply to antibiotic resistance protocol, is factible to evaluate de heavy metal tolerance. In the following link you will be found the basis of this kind of tests
http://textbookofbacteriology.net/resantimicrobial.html

"Implications of Microbial Heavy Metal Tolerance in the Environment"
http://www.ruf.rice.edu/~rur/issue2_files/PDF_Final/spain.pdf

"Heavy-Metal and Antibiotic Resistance in the Bacterial Flora of Sediments of New York Bight"
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC243070/pdf/aem00212-0077.pdf