Microbiology is a broad term which includes virology, mycology, parasitology, bacteriology, immunology and other branches.

• Shagufta Liaqat

  What are the different methods to prevent proteus swarming on any culture media????

  Swarmming of proteus on the whole agar plate
• Rosa Elena Cordero-Reyes

  Which the best culture media for thermophiles?

  The water contains sulfates and carbonates... Thanks

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    Henson Jebajose replied

    Neha, As B.patel said don't complicate it.You can follow above statement or Please go through APHA Book May be chapter 23 or 24. That will use full for you to isolate and identifyNeha, As B.patel said don't complicate it.You can follow above statement or Please go through APHA Book May be chapter 23 or 24. That will use full for you to isolate and identify Thermophilic acidophilic bactera.Since you have to identify the Thermophlic you must give a heat shock in the respective diluent then go for the Plating. APHA will guide you clearly.

    2 days ago
• Retno Nurhayati

  Aerobic formate-utilizing bacteria/microorganism

  I have searched for reports about microorganisms which can utilize formate as the sole carbon source in aerobic condition. I found that only syntrophic bacteria (usually mix between methanogenI have searched for reports about microorganisms which can utilize formate as the sole carbon source in aerobic condition. I found that only syntrophic bacteria (usually mix between methanogen bacteria and hydrogen consuming bacteria) which have such ability. Is there any report about single bacteria/microorganism which can utilize formate as solely carbon source aerobically? Thanks

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    Dimitry Yu Sorokin replied

    Of course, plenty of them. This are C1-utilizing methylotrophs, for example. I personally worked with a carboxydotrophic haloalkalphiles which also grew aerobically on formate, genera AlkalispirillumOf course, plenty of them. This are C1-utilizing methylotrophs, for example. I personally worked with a carboxydotrophic haloalkalphiles which also grew aerobically on formate, genera Alkalispirillum and Alkalilimnicola from the Gamma. All such bacteria actually growing autotrophically, i.e. they oxidize formate as e-donor and use CO2 as carbon surce

    4 days ago
• Prema Viswanath

  Catalase enzyme isolation from bacteria

  I want to use catalase enzyme to beakdown hydrogen peroxide that is added to milk. I would appreciate any suggestions on the best bacterium to get this enzyme from.

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    Prema Viswanath replied

    Thanks a lot Peter. I understand that if I grew it in a synthetic medium, harvested the cells and then lysed the cells, I could get some crude enzyme.

    4 days ago
• Michel Laramee

  Bacterial flagella staining method

  I am looking for a very good method and protocol for staining bacteria's flagella and capsules, could you help me out?

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    Mohammed Shannaq replied

    You can do the procedure present in this article : a method of staining bacterial flagella and capsules together with a study of the origin of flagella

    6 days ago
• Juan Cristóbal Jiménez

  Has anybody tried to develop a "One-step deletion of genes using PCR products" (like the Lambda-Red recombination system) for Gram positives?

  In E. coli and related species you can use the Lambda-Red system developed by Datsenko & Wanner to generate allelic recombination, with just 20 bp homology regions in each end of your PCR product.In E. coli and related species you can use the Lambda-Red system developed by Datsenko & Wanner to generate allelic recombination, with just 20 bp homology regions in each end of your PCR product. Wondering if there was something similar for Gram positives?

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    Salvatore Mandalà replied

    Try to search for anything concerning the Redirect Technology for Streptomyces. http://www.ncbi.nlm.nih.gov/pubmed/15251278 http://www.ncbi.nlm.nih.gov/pubmed/12563033

    6 days ago
• Bastian Herzog

  What media could be best for the isolation of specific microorganisms that are able to degrade Benzotriazoles from activated sludge?

  I am trying to isolate microorganisms from enriched activated sludge that are able to degrade benzotriazoles. The enriched mixed culture is able to degrade the desired compounds but I failed till nowI am trying to isolate microorganisms from enriched activated sludge that are able to degrade benzotriazoles. The enriched mixed culture is able to degrade the desired compounds but I failed till now to identify the organism that is responsible for that. Does someone know a good isolation method or a good (solid) enrichment media? I tried R2A, LB, THB, and some other minimal media without any success. Thanks a lot for helping me.

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    Mohammed Shannaq replied

    you can use N.A media and cover surface with benzotrizoles and streak with isolated bacteria then incubate at 30c for 7 days, if you see hallo zone around colonies, your results positive.

    7 days ago
• Sameena Khan

  Difference between Hemolysis and Hemodigestion?

  When we see a blood agar plate in transmitted light how can we diffentiate between hemolysis and hemodigestion?How will we differentiate transparent and translucent in NA plate?
• Dixon Davis

  How could we determine the effectivness of a particular group of bacteria in decomposition?

  A large number of bacterias are involved in vermicomposting. From that large group how it is possible to find a single bacterial family which shows the maximum decomposition rate?

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    Dixon Davis replied

    Thanks ridzky...but other than the c/n ...can you suggest anything else....

    10 days ago
• Niti Jadeja

  Is anyone dealing with Metagenomic library construction from sludge DNA ?

  I am to begin one such task and have decided on Bac as my cloning vehicle, and restriction digestion as the next step using BamH1 and pst1.

  Recent replies ⋅ Show All (3)

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    David Hayes replied

    Hi Niti, http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3235511/ I think this will link to an article which provides an earlier 'fundamental' ref'ce (Rondon et al., 2000 AEM) to BAC vector metagenomicHi Niti, http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3235511/ I think this will link to an article which provides an earlier 'fundamental' ref'ce (Rondon et al., 2000 AEM) to BAC vector metagenomic libraries for detection of enzyme activity. I'll assume you have already looked through these. Never know!!!! Maybe post your query on linkedin also? Good Luck.

    11 days ago
• Gunjan Pandey

  Can anyone explain the difference between Bile Esculin Agar and Esculin Iron Agar used in Esculin Hydrolysis Test?

  The composition of both the Chemicals is almost same except for the presence of Ferric Citrate in the former and Ferric ammonium citrate in the latter.
• Henson Jebajose

  Guidelines about the Production of antibiotics or Vitamins by Genotype modulation in Fungi?

  I am looking to change the gene sequence to increase the rate of production. Please suggest a reliable method (Mutation or Recombinant , etc)

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  • 
    Sidek Ahmad replied

    Pichia would be a good choice to start with

    13 days ago
• Shirin Qureshi

  Which are the online free access journals for microbiology?

  I am new to this subject, and want to identify bacteria and fungi.

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    Shirin Qureshi replied

    Thank you all..

    18 days ago
• Mohsen Arefnejad

  Correlation of growth rates of environmental and clinical bacteria.

  Could anybody tell me what correlation there is between the growth rates of environmental and clinical bacteria ( one strain)? If I found rate is the same or different, what is your conclusion? ICould anybody tell me what correlation there is between the growth rates of environmental and clinical bacteria ( one strain)? If I found rate is the same or different, what is your conclusion? I know this rate is affected in a host, for example this rate for clinical isolates in vivo is depend on acute or sever, nutrition, immune system and so on, but I want to understand the correlation between the environmental and clinical rates I get invitro.

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    Mohsen Arefnejad replied

    Thanks again Apurva Pathak.

    19 days ago
• Natalie Haddad

  Surface area of Listeria monocytogenes covered in Internalin A

  Does anyone know what percentage of Listeria monocytogenes surface area is covered in the protein, Internalin A (InlA), which induces phagocytosis of L. monocytogenes into human epithelial cells? ForDoes anyone know what percentage of Listeria monocytogenes surface area is covered in the protein, Internalin A (InlA), which induces phagocytosis of L. monocytogenes into human epithelial cells? For example, how many molecules of Internalin A per square unit of L. monocytogenes?

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    Hélène Marquis replied

    Dear Nathalie, I don't have the answer to your question, but I would recommend that you ask Marc Lecuit from pasteur Institute. He is the expert in Internalin A. Helene

    23 days ago