Molecular Biology of the Microbes

• Yasemin Sertel

  Genomic DNA isolation from Aspergillus niger

  I have isolated genomic DNA from Aspergillus niger. After the last part of isolation, TE Buffer addition (dilution process), the TE Buffer is still black due to pigment. Pigment of A.niger can't beenI have isolated genomic DNA from Aspergillus niger. After the last part of isolation, TE Buffer addition (dilution process), the TE Buffer is still black due to pigment. Pigment of A.niger can't been removed. How can I remove this pigment? Could anybody send me protocol for genomic DNA isolation from A. niger?

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    Praveen Rahi replied

    That's grt you got solution for your problem...

    25 days ago
• Kamla Kant

  how to isolate DNA of mycobacteria mannualy ?

  i want to isolate DNA of mycobacteria by mannual method from growth on LJ media for further molicular studies. how can i extract the DNA and send them to another laboratory for identification andi want to isolate DNA of mycobacteria by mannual method from growth on LJ media for further molicular studies. how can i extract the DNA and send them to another laboratory for identification and sequencing ?
• David Ngmenterebo

  Which selective antibiotic marker can be used to construct a vector for the deletion of genes in a Klebsiella pneumoniae homomlogous recombination?

  I want to to delete a cluster of genes that code for uncharacterised proteins in Klebsiella pneumoniae strains by homologous recombination, and I want a good antibiotic marker to construct the vecorI want to to delete a cluster of genes that code for uncharacterised proteins in Klebsiella pneumoniae strains by homologous recombination, and I want a good antibiotic marker to construct the vecor for this purpose. Also, I will use this marker to screen for my mutants. I will equally be glad if you can suggest the type of vector I should use for the antibiotic marker.

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    David Ngmenterebo replied

    Thank you all for your wonderful ideas, you are great friends in need

    Apr 20, 2012
• Vijayalakshmi Gunasekaran

  Proteome extraction from Bacterial consortium?

  I am looking for a protocol to extract the total proteome from a bacterial consortium which consists of both gram positive and negative strains, could you help me out?

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    jp Dangy replied

    The second method generates clearly much more materials extracted. The pattern that you see is kind of normal since you generate a random collection of proteins all at different size so it canThe second method generates clearly much more materials extracted. The pattern that you see is kind of normal since you generate a random collection of proteins all at different size so it can appears as a smeary pattern. Do you see discrete band in your gel when you stain with commassie blue ? I would repeat your gel and load less material ans separate the samples away from each other to avoid distorsion of the lines, remember to load sample buffer only in the empty wells. Take a color photo and we will see ! Another control that you can do is to transfer your extracts on a western blot and probe with an antibody to see whether you can recognize known proteins

    Apr 17, 2012
• Ly Tran

  How to break the chains of lactis?

  I'm using particle tracking to investigate the lactis transport through mucus, but these strains that I'm using form a chain, i.e 3-4 cells combine to each other as a line. Do you have any idea toI'm using particle tracking to investigate the lactis transport through mucus, but these strains that I'm using form a chain, i.e 3-4 cells combine to each other as a line. Do you have any idea to break the chain without affecting on lactis cells?

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    Ly Tran replied

    Shingo Hino: as I know Lb. rhamnosus GG persist in human gastrointestinal track for one week

    Mar 30, 2012
• Asmaa Kasem jbr

  Electron microscopic study of biofilm matrix

  Can we identify the matrix of biofilm or composition of the matrix (polysaccharides, proteins etc.) by using electron microscopy, or can anyone recommend a procedure to study the matrix?

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    Asmaa Kasem jbr replied

    Thanks all, i working on klebsiella biofilm , and I need to identify the composition (protein , polyscchride and ets ) of biofilm matix of klebsiella pneumoniae to degrade this biofilm , i found aThanks all, i working on klebsiella biofilm , and I need to identify the composition (protein , polyscchride and ets ) of biofilm matix of klebsiella pneumoniae to degrade this biofilm , i found a method to extract EPS of Pseudomonas fluorescens and Alcaligenes denitrificans from paper that cited ( the protective effect of gluteraldehyde in the extraction of EPS from biofilm ) by Joana Azeredo, Mariana Henriques, Sanna Sillankorva and Rosário Oliveira , but it seems to be difficult , that we must to keep the cells alive , so if you can help , and i will very appreciated

    Mar 24, 2012
• Gyaben kofi darko

  Aspergillus fumigatus

  After I have isolated the fungi, how do I characterize the aspergillus fumigatus from the isolate?

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    Preeti Aahir replied

    Take 2 g of soil,and dissolve it in 8 ml 0.2 M NaCl-1% Tween 20. Take 100 μl from this and plate on Sabouraud dextrose agar or Aspergine agar plates. Incubated them at 37°C for 24-36 hours. FurtherTake 2 g of soil,and dissolve it in 8 ml 0.2 M NaCl-1% Tween 20. Take 100 μl from this and plate on Sabouraud dextrose agar or Aspergine agar plates. Incubated them at 37°C for 24-36 hours. Further for single spore culture you can u can streak the diluted spores on SDA plates for 12-24 hours at 37°C. Aspergillus colonies can be identified as green to gray color and under microscope conidiophores appears as vesicle flask shape with series of sterigmata, each bearing a chain of unicellular smooth, globular conidia. http://www.aspergillus.org.uk/indexhome.htm?secure/speciesdatabase/fumigatus/fumicult.php~main.

    Jan 5, 2012

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• Garima Srivastava

  oleoginous fungus

  I 'm working on biodiesel production from fungi, if you have any information regarding to that please give your opinion

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    Rahul Allapure replied

    some fungal taxa from ascomycetes r able for this research

    Jul 18, 2011
• Nguyen Linh

  Electroporation for marine bacteria, Help me!!!

  Dear friends, I hope to get your comments for my trouble now. I want to introduce transposon Tn5 to marine bacteria. I think electroporation is the effective and simple way to introduce DNA intoDear friends, I hope to get your comments for my trouble now. I want to introduce transposon Tn5 to marine bacteria. I think electroporation is the effective and simple way to introduce DNA into host livin cells. But now I get trouble. Before electroporation, it need to wash cell by poration buffer and then resuspend cells in the buffer. My marine bacteria seemed to be very sensitive. Normally, cell will be washed by ddH2O containing 10% glycerol. I tried to take colony from agar plate and suspend into ddH2O containing 10% glycerol, and then spread on the agar plate. But no colony appeared on the plate after incubation. I think my bacteria were lysed in this condition (no salt). I also tried to use other electroporation buffers containing MgSO4, sucrose, HEPES, glycerol. And I aslo checked bacterial viability under this condition. But no colony appeared. Now I donot know how to treat this marine bacteria suitably before electroporation. Can someone help me? Thanks alot

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• Damrongsak Arlai

  Fluorescence In Situ Hybridization

  I am interesting topic for study pathogen in food.
• Amola Alfrado

  bioremediation ?

  what the difference between traditional methods and molecular biology methods in bioremediation ?

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    Chinnathambi Velu replied

    which are the countries are focusing research on bioremediation ?

    Jun 20, 2011