• Agnieszka Barbara Najda added an answer:
    What is the retention time of lactoferrin when using TFA and acetonitrile as mobile phase in a reverse phase C18 column?

    when I use C18 Reverse phase column , TFA and acetonitrile as mobile phase , gradient elution at a flow rate of 0.5 ml/min , the retention time is around 3.58 mins and I am not sure about the peaks that are eluted , when i compare it with the whey I get the same pattern of peaks but at aroun 5.45 mins. Can someone help me how to analyse with the same. Thank you

    Agnieszka Barbara Najda

    I agree with the opinion Jandirk Sendker.
    But also important are the parameters for whom he recalls Wołodymyr Skalka. 
    Are internal pattern, also you get kakie results? The matrix of whey may also cause some displacement. But what is most important, then check whether your gradjent is well configured.

  • Mohammed Al Marjani added an answer:
    What is the effect of lactoferrin on MDR bacteria?

    What is the effect of lactoferrin on Multidrug resistant bacteria?

    Mohammed Al Marjani

    Thank you Traci for your helping

  • Aletheia Lima Souza added an answer:
    Does anyone know if I can reverse denaturation by urea (7M)?

    I am working with lactoferrin and over my protocol for expression in vitro I have been used urea (7M) buffer for denaturation, however, I would like to proceed an assay with my lactoferrin and it should be intact. Can I reverse denaturation by urea of my lactoferrin? I appreciate any idea.


    Aletheia Lima Souza

    Hey all, I performed my assay, and right now I am waiting for the results.

    Thank you guys for your reply. It was ver helpful.

    Obrigada Noelita, eu estou plotando os dados pra ver se a proteina mantem a atividade. Muito bom ter noticias suas. Bjao amiga :-).

    Jorge, amigo, valeu a dica. QQ coisa bato um fio pra ti. A gnt segue aqui com os experimentos. Bjao

  • Vesna Karapetkovska Hristova added an answer:
    What are the conditions of RP-HPLC for lactoferrin and lysozyme detection?

    Can anyone suggest how I can prepare samples (cow's milk) and protein standards (lactoferrin and lysozyme) for RP-HPLC? What conditions (mobile phase, gradient, operating temperature, flow rate) I can use for RP-HPLC whey protein detection?

    Vesna Karapetkovska Hristova

    Dear Kristina!

    I m wishing to you a BIG success in this hard work, but believe me, here in Macedonia also we have problems with lactoferin detection in colostrum, in normal milk etc, and also a lot of money for this....I agree with d-r Bianchi, try to do this by ELISA instrument, ask in your local


  • Prof. Ravi K. Sharma added an answer:
    Does Bovine lactoferrin have antioxidant effect?

    lactoferrin is used as immunostimulant

    Prof. Ravi K. Sharma

    A Google search on "Bovine lactoferrin antioxidant pdf returned about 2,14,000 hits

    Antioxidant Effects of Bovine Lactoferrin on Dexamethasone ...

    first page dump:
    by L Safaeian - ‎2014 - ‎Related articles
    Nov 19, 2013 - International Scholarly Research Notices is a peer-reviewed, open access journal covering a wide range of subjects in science, technology, ...
    Missing: search
    Effect of selenium-saturated bovine lactoferrin (Se-bLF) - DRO
    by H Burrow - ‎2011 - ‎Cited by 6 - ‎Related articles
    Apr 5, 2012 - bovine lactoferrin ... The states of all antioxidant enzymes (glutathione peroxidase (GPx), glutathione reductase (GR), ... Search Google Scholar.
    Antioxidant enzyme activities of iron-saturated bovine ... - DRO
    by H Burrow - ‎2011 - ‎Cited by 17 - ‎Related articles
    Dec 5, 2011 - Antioxidant enzyme activities of iron-saturated bovine lactoferrin (Fe-bLf) in human gut epithelial cells under ... Search Google Scholar.
    Patent CA2141961C - Antioxidant - Google Patents
    The present invention provides a safe antioxidant applicable for food, drug medicine, non-medical drug and other products. ... Advanced Patent Search .... Kogyo Campany), bovine lactoferrin (manufactured by Sigma Company), hydrolysate of ...
    Patent US7326775 - purification of transferrins ... - Google
    Feb 5, 2008 - Advanced Patent Search ... 3) purifying said antioxidant-treated lactoferrin with at least one polyphenol to form purified lactoferrin; and .... Oral administration of bovine LF (40 mg/day) in healthy human volunteers (n=17) ...
    lactoferrin 339615-76-8 - The Good Scents Company
    Food Additive : Functional use(s) - antioxidants. ... Scientific Opinion on bovine lactoferrin:page or pdf ... Google Scholar : Search, Google Books : Search.
    Lactoferrin Cancer Miracle- Super Immune-Booster - percys ...
    Dec 12, 2014 - Your ads will be inserted here by. Google Adsense. .... antioxidant activity of an oral supplementation of bovine lactoferrin in humans. Using an
    Lactoferrin - Advanced Health and Life Extension
    Lactoferrin boosts immune function and has antioxidant properties. ... Advanced Health & Life Extension. Google. Custom Search ... The lactoferrin concentration in bovine (cows) milk is only 0.5% to 1.0% while human breast milk can contain ...
    Full Text - Poultry Science - Oxford Journals
    by L Wang - ‎2008 - ‎Cited by 24 - ‎Related articles
    Institution: Google Indexer; Sign In as Personal Subscriber ..... (2008) found the similar effect of bovine lactoferrin, an antioxidant, can improve antioxidant ...
    Patent US4668771 - Method for separating bovine ... - Google
    Advanced Patent Search ... Method for separating bovine lactoferrin from cow's milk and purifying same ... A method as claimed in claim 1 wherein the bovine lactoferrin adsorbed to the ..... US7326775, 2 Dec 2005, 5 Feb 2008, En-N-Tech, Inc. purification of transferrins using surfactants, antioxidants and flavonoids, than ...

  • Maged El-Ashker added an answer:
    Does any body know of a Company that produces bovine lactoferrine, protected fish oil and equine recombinant IL-7?

    Shall any body inform me by the source for these products, I appreciate his/her kind help.

    Maged El-Ashker

    Thank you so much Mr. Saad for your Answer, I will try this.

About Lactoferrin

Lactoferrin (LF), also known as lactotransferrin (LTF), is a multifunctional protein of the transferrin family. Lactoferrin is a globular glycoprotein with a molecular mass of about 80 kDa that is widely represented in various secretory fluids, such as milk, saliva, tears, and nasal secretions.

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