Aletheia Lima Souza added an answer:Does anyone know if I can reverse denaturation by urea (7M)?
I am working with lactoferrin and over my protocol for expression in vitro I have been used urea (7M) buffer for denaturation, however, I would like to proceed an assay with my lactoferrin and it should be intact. Can I reverse denaturation by urea of my lactoferrin? I appreciate any idea.
Hey all, I performed my assay, and right now I am waiting for the results.
Thank you guys for your reply. It was ver helpful.
Obrigada Noelita, eu estou plotando os dados pra ver se a proteina mantem a atividade. Muito bom ter noticias suas. Bjao amiga :-).
Jorge, amigo, valeu a dica. QQ coisa bato um fio pra ti. A gnt segue aqui com os experimentos. BjaoFollowing
Vesna Karapetkovska Hristova added an answer:What are the conditions of RP-HPLC for lactoferrin and lysozyme detection?
Can anyone suggest how I can prepare samples (cow's milk) and protein standards (lactoferrin and lysozyme) for RP-HPLC? What conditions (mobile phase, gradient, operating temperature, flow rate) I can use for RP-HPLC whey protein detection?
I m wishing to you a BIG success in this hard work, but believe me, here in Macedonia also we have problems with lactoferin detection in colostrum, in normal milk etc, and also a lot of money for this....I agree with d-r Bianchi, try to do this by ELISA instrument, ask in your local laboratories........best
Prof. Ravi K. Sharma added an answer:Does Bovine lactoferrin have antioxidant effect?
lactoferrin is used as immunostimulant
A Google search on "Bovine lactoferrin antioxidant pdf returned about 2,14,000 hits
Antioxidant Effects of Bovine Lactoferrin on Dexamethasone ...
first page dump:
by L Safaeian - 2014 - Related articles
Nov 19, 2013 - International Scholarly Research Notices is a peer-reviewed, open access journal covering a wide range of subjects in science, technology, ...
Effect of selenium-saturated bovine lactoferrin (Se-bLF) - DRO
by H Burrow - 2011 - Cited by 6 - Related articles
Apr 5, 2012 - bovine lactoferrin ... The states of all antioxidant enzymes (glutathione peroxidase (GPx), glutathione reductase (GR), ... Search Google Scholar.
Antioxidant enzyme activities of iron-saturated bovine ... - DRO
by H Burrow - 2011 - Cited by 17 - Related articles
Dec 5, 2011 - Antioxidant enzyme activities of iron-saturated bovine lactoferrin (Fe-bLf) in human gut epithelial cells under ... Search Google Scholar.
Patent CA2141961C - Antioxidant - Google Patents
The present invention provides a safe antioxidant applicable for food, drug medicine, non-medical drug and other products. ... Advanced Patent Search .... Kogyo Campany), bovine lactoferrin (manufactured by Sigma Company), hydrolysate of ...
Patent US7326775 - purification of transferrins ... - Google
Feb 5, 2008 - Advanced Patent Search ... 3) purifying said antioxidant-treated lactoferrin with at least one polyphenol to form purified lactoferrin; and .... Oral administration of bovine LF (40 mg/day) in healthy human volunteers (n=17) ...
lactoferrin 339615-76-8 - The Good Scents Company
Food Additive : Functional use(s) - antioxidants. ... Scientific Opinion on bovine lactoferrin:page or pdf ... Google Scholar : Search, Google Books : Search.
Lactoferrin Cancer Miracle- Super Immune-Booster - percys ...
Dec 12, 2014 - Your ads will be inserted here by. Google Adsense. .... antioxidant activity of an oral supplementation of bovine lactoferrin in humans. Using an
Lactoferrin - Advanced Health and Life Extension
Lactoferrin boosts immune function and has antioxidant properties. ... Advanced Health & Life Extension. Google. Custom Search ... The lactoferrin concentration in bovine (cows) milk is only 0.5% to 1.0% while human breast milk can contain ...
Full Text - Poultry Science - Oxford Journals
by L Wang - 2008 - Cited by 24 - Related articles
Institution: Google Indexer; Sign In as Personal Subscriber ..... (2008) found the similar effect of bovine lactoferrin, an antioxidant, can improve antioxidant ...
Patent US4668771 - Method for separating bovine ... - Google
Advanced Patent Search ... Method for separating bovine lactoferrin from cow's milk and purifying same ... A method as claimed in claim 1 wherein the bovine lactoferrin adsorbed to the ..... US7326775, 2 Dec 2005, 5 Feb 2008, En-N-Tech, Inc. purification of transferrins using surfactants, antioxidants and flavonoids, than ...Following
Maged El-Ashker added an answer:Does any body know of a Company that produces bovine lactoferrine, protected fish oil and equine recombinant IL-7?
Shall any body inform me by the source for these products, I appreciate his/her kind help.
Thank you so much Mr. Saad for your Answer, I will try this.Following
Lactoferrin (LF), also known as lactotransferrin (LTF), is a multifunctional protein of the transferrin family. Lactoferrin is a globular glycoprotein with a molecular mass of about 80 kDa that is widely represented in various secretory fluids, such as milk, saliva, tears, and nasal secretions.