This group is for discussion about any influenza research related topics

• Eva Emmoth

  What is the advantage of isolating swine influenza virus on primary porcine kidney cells instead of on MDCK-cells (with trypsin)?

  Isolation of swine influenza virus

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  • 
    Peter J Krell replied

    MDCK cells can be used for isolation of swine influenza and for routine work on it. Primary cultures do have the advantage of being the "homologous" system but suffers from increased labour to makeMDCK cells can be used for isolation of swine influenza and for routine work on it. Primary cultures do have the advantage of being the "homologous" system but suffers from increased labour to make the cells and the possibility of the primary cultures containing other agents, e.g. non cytopathic viruses

    1 hour ago
• Nandhini Bhashini

  Is it possible to cultivate seasonal influenza isolates in MDCK cell lines instead of TPCK? And is it possible to use media without serum and without receptor destroying enzyme?

  As of my understanding, the three items are important for inf(influenza) cultivation, but i am yet to set up an order to get those. As i am already cultivating herpes in normal MDCK cell lines, IAs of my understanding, the three items are important for inf(influenza) cultivation, but i am yet to set up an order to get those. As i am already cultivating herpes in normal MDCK cell lines, I thought I could give it a try to grow seasonal inf isolate,if not a swine inf isolate.Does anyone have experience with that?

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  • 
    Irona Khandaker replied

    Thanks for your link, Mr.Thomas Rowe

    11 days ago
• Nandhini Bhashini

  Apart from Denka Seiken, where all can we purchase "RDE II" Receptor destroying enzyme of Vibrio cholerae origin?

  Which manufacturer's RDE are less expensive than Denka Seiken?
• Eva Emmoth

  Influenza growth curves

  We have made growth curves for swine influenza virus in swine primary cells with MOI 0.1, and titrered on MDCK cells. Maximum titres reached about 5 log10 TCID50 per 50 µl cell supernatant after 5We have made growth curves for swine influenza virus in swine primary cells with MOI 0.1, and titrered on MDCK cells. Maximum titres reached about 5 log10 TCID50 per 50 µl cell supernatant after 5 days, then decreased about 2 log10 up to 7 days. After that the titre increase again about 2 log10. What could be the reason for the late increase, since then probably all cells are dead?

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  • 
    Eric Frost replied

    Influenza virus requires 2 enzymes to become active. the neuraminidase of the virus will digest host receptors and free virus that has been clumped together. Clumped virus would give the impressionInfluenza virus requires 2 enzymes to become active. the neuraminidase of the virus will digest host receptors and free virus that has been clumped together. Clumped virus would give the impression of lower titre. the hemagglutinin also must be cleaved by a trypsin like activity to give HA1 and HA2. Otherwise the virus is not infectious. I would suspect one of these 2 enzymes is the cause of your late rise in titre. To be absolutely sure, you could titrate the viral RNA. You probably should treat your extracts with a small amount of trypsin before infection as we do with clinical samples.

    25 days ago
• MARIA SERENA Beato

  RDE treated sera

  How long can I store RDE treated sera at -20°C?

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    MARIA SERENA Beato replied

    Thanks,we generally treat the minimum amount to carry the test but if we could avoid to treat sera every time and do just once it would be easier. thanks a lot.

    Mar 23, 2012
• Soumendranath Bhakat

  Anyone interested in working on the above mentioned project regarding deign of new Anti-influenza agents please contact by email:soumendranath2009@gmail.com

  New Designed Drugs for H5N1,H1N1 and influenza.doc

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  • 
    Binod Kumar replied

    Can we have a collaborative work?

    Mar 14, 2012
• MARIA SERENA Beato

  In vivo studies with mice and influenza

  Has anyone experience with antibody response of mice following experimental infection with influenza? I am looking to share info about the antibody response on mice compared by HI,Has anyone experience with antibody response of mice following experimental infection with influenza? I am looking to share info about the antibody response on mice compared by HI, micro-neutralisation and ELISA test. Thank you

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    MARIA SERENA Beato replied

    Thanks a lot.

    Mar 11, 2012
• Soumendranath Bhakat

  I have some molecules which I think will work against H5N1,H1N!1 and other influenza types if anyone interested in seeing these molecules and is interested in collaborative research please contactI have some molecules which I think will work against H5N1,H1N!1 and other influenza types if anyone interested in seeing these molecules and is interested in collaborative research please contact soumendranath2009@gmail.com

  New Designed Drugs for H5N1,H1N1 and influenza.doc

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  • 
    Anil Prasad replied

    It is serious and needs careful rethinking about H5N!, Bird flu virus infection in human. I fully agree that the bird reservoir fist needs to be eliminated and the human contact reduced to nil. EachIt is serious and needs careful rethinking about H5N!, Bird flu virus infection in human. I fully agree that the bird reservoir fist needs to be eliminated and the human contact reduced to nil. Each human case to be segregated and treated with utmost care not to allow any further spread. The local Government and WHO should handle this on top priority for the disease containment. A K Prasad.

    Mar 1, 2012

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• Shakti Singh

  Pandemic H1N1 2009

  Is H1N1 really a pandemic or it is the beginning process in the making of a pandemic???

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• Veronica Koay

  So far, I heard about vaccine targeting on hemagglutinin protein, I'm curious to know any vaccine is targeting on neuraminidase protein?

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  • 
    Eric Frost replied

    Human influenza vaccines contain both the hemagglutinin and the neuraminidsase proteins

    Feb 7, 2012
• Veronica Koay

  Hey guys, do you know any software I can use to predict the residues that are prone to mutation?
• Ijad Madisch

  Welcome Influenza researchers

  Dear researchers, I hope this group can facilitate spillovers of information according to influenza research. Thanks for joining Best Ijad

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  • 
    Veronica Koay replied

    could I know how to check? Am I right to say that the sequence identity percentage will partition the sequences into a certain clade / subclade? Sequences within the clade will have certain residuecould I know how to check? Am I right to say that the sequence identity percentage will partition the sequences into a certain clade / subclade? Sequences within the clade will have certain residue at a particular position while others do not have.

    Feb 7, 2012