Environmental Biotechnology

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10 How to estimate sulfur in the water sample?
By Puhazh Selvan · Anna University of Technology, Coimbatore

Pierre-Jean Superville · Université des Sciences et Technologies de Lille 1

Voltammetry with the hanging mercury drop electrode is rather sensitive for elemental sulfur determination. Here are some links : http://www.sciencedi... [more]

Voltammetry with the hanging mercury drop electrode is rather sensitive for elemental sulfur determination. Here are some links : http://www.sciencedirect.com/science/article/pii/0003267092850182 http://www.sciencedirect.com/science/article/pii/S003991401300194X

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2 How to synthesis a Fe and Ni , or Fe/Ni nano particles using anaerobic microorganism ( which is involved in hydrogen production)?
By Sivagurunathan Periyasamy · Feng Chia University

Alejandro Pérez Larios · Universidad de Guadalajara

You can be the synthesis using the precipitation-depositation method.

You can be the synthesis using the precipitation-depositation method.

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12 Looking for researchers with an interest in the valorization of asparagus waste. Does anyone have experience with products based on asparagus waste?
By Catharina Meyer · Hochschule RheinMain

Martin Lemmen · UTS Biogastechnik

To add to my previous answer, I'd start with a Weender fodder analysis, as this way you could get a good estimate of the Biogas yield without doing a ... [more]

To add to my previous answer, I'd start with a Weender fodder analysis, as this way you could get a good estimate of the Biogas yield without doing a (more expensive) gas yield test. What's beeing done with the wastes now?

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5 Very low A260/A230 ratio in extracted DNA from sludge sample using FastDNA SPIN kit for soil.
By Teck Nam Ang · University of Malaya

Fabio Chinalia · Universidade Federal da Bahia

There are a few strategies you can use for equating the presence of humic substances and other potential PCR inhibitors. Sample dilution is one fast c... [more]

There are a few strategies you can use for equating the presence of humic substances and other potential PCR inhibitors. Sample dilution is one fast choice, but, when this does not produce a good result, you may try running the total DNA extract on agarose. This will separate humic substances from DNA and you will recover DNA from the gel by cutting the band, melting the agarose and precipitate DNA with ice cold ethanol (however, there are kits for that too).

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2 How to prepare glycerol stock for anaerobic bacteria?
By Puhazh Selvan · Anna University of Technology, Coimbatore

Budak kuantan · Universiti Malaysia Pahang

materials 20 mM Phosphate buffer pH 7 Resazurine solution (500 mg/l) Glycerol ~100 mM titanium citrate solution (filter‐sterilized) 10... [more]

materials 20 mM Phosphate buffer pH 7 Resazurine solution (500 mg/l) Glycerol ~100 mM titanium citrate solution (filter‐sterilized) 10 ml serum bottles(Sigma)) Butyl stoppers and crimp caps Method Prepare 100 ml phosphate solution Add resazurine (final concentration 0.5 mg/l) Bring the solution to a full boil and cool down under N2 stream Add 100 ml glycerol; this makes a 50% (v/v) glycerol solution Fill the anaerobic vials with 4 ml of the glycerol solution Stopper and cap them Change gas phase to 100% N2 or 80/20% N2/CO2; fill with 0.2 atm overpressure Autoclave the vials Inject 3 – 4 ml of a late log phase bacterial culture to the sterilized glycerol solution using a 5ml syringe. Final concentration of the glycerol should be 25 – 30 % Using 1 ml syringe, inject aprox. 3 drops of filter sterilized titanium citrate solution to each vial to reduce the stocks Freeze them at –80c

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1 How to effectively PCR amplify DNA recovered from aquatic environments?
By Jun Hamabe · Tokyo University of Agriculture and Technology

Amit Kumar · Stazione Zoologica Anton Dohrn di Napoli

you can use 0.5 microliter of 1mg/ml concentration of BSA besides all necessary reagents for the PCR amplification. Generally avoid using large concen... [more]

you can use 0.5 microliter of 1mg/ml concentration of BSA besides all necessary reagents for the PCR amplification. Generally avoid using large concentration of template DNA and increase accordingly the result obtained with the less template. Always use a negative control in order to check the correct amplification of target microbial communities.. if you are using a universal bacterial primer be sure to work in a clean environment and always with gloves. Make aliquotes of reagents and discard if any contamination occurs.

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2 How can I compare the energy yield between Anaerobic digestions (metane) and MFC power generations?
By Ramesh Kakarla · Kyung Hee University

Ramesh Kakarla · Kyung Hee University

Dear Jan Arends....Thank you , I had completed the task.

Dear Jan Arends....Thank you , I had completed the task.

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Question:
Open Cultivating ascidian cells?
Does anybody have some experiences in cultivating cells from ascidians? I have found a protocol (see attachment) that explains the basic steps in esta... [more]

Does anybody have some experiences in cultivating cells from ascidians? I have found a protocol (see attachment) that explains the basic steps in establishing a primary cell cultures for Ciona intestinalis. Anybody here who successfully used a similar protocol to cultivate cells of other ascidians? Does it work?

Development-of-primary-cell-cultures-from-Ciona-intestinalis-explant.pdf ×

By Lars Behrendt · University of Copenhagen

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Question:
Open Biological nutrient removal – A2O process
In A2O (Anaerobic Anoxic Aerobic) process of BNR, MLSS concentration in all reactors will remain the same or could be possible to maintain diffe... [more]

In A2O (Anaerobic Anoxic Aerobic) process of BNR, MLSS concentration in all reactors will remain the same or could be possible to maintain different as required?

By Sandip Magdum · Amity University

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17 Does anybody know where we could buy xylan from oat spelt?
By Kristin Kaiser · Georg-August-Universität Göttingen

John Nduko · Hokkaido University

we do have xylan from oat spelts in our lab. it was bought from sigma aldrich company. You can try!!

we do have xylan from oat spelts in our lab. it was bought from sigma aldrich company. You can try!!

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6 How to guarantee the sampling effort for detecting the diversity of endophytic fungi?
By Marcela Ordoñez · National University of Colombia

Prakash Santhiyagu · SRM University

the diversity of AMF fungi depend of crops samples and sediment also environmental and nutritional character was influenced the diversity variation. I... [more]

the diversity of AMF fungi depend of crops samples and sediment also environmental and nutritional character was influenced the diversity variation. If you may consider the three more sampling points and isolated the AFM fungi one crop species through out year. it helps to frequently occur fungi. How they varied due to the environmental parameters.

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1 Impact of PVA containing wastewater on membrane fouling
By Janardhan Bornare · Sant Gadge Baba Amravati University

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2 What will be the minimum HRT for 0.5 % PVA (Polyvinyl alcohol) containing Textile Effluent with 15000 to 18000 mg/l COD?
By Sandip Magdum · Amity University

Sandip Magdum · Amity University

Thank you Paul. As PVA used in this case with a degree of polymerization 1700-1800 and having a molecular weight approximately 14,000 g/mol and also ... [more]

Thank you Paul. As PVA used in this case with a degree of polymerization 1700-1800 and having a molecular weight approximately 14,000 g/mol and also wish to mention that, after treatment of PVA containing effluent, textile industry expects to meet stringent norms of COD <100 mg/l. Textile industry is / will not be interested to use UF or RO membrane filtration to concentrate the suspended solids and reuse, as there is a high effluent quantity, so high capital investment required and they are not interested in reuse the recycled PVA. In this case, biodegradation is a good option, but high HRT may increase cost of treatment which is the main concern.

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2 Latest information on chalcopyrite heap bioleaching?
By Sandeep Panda · Institute of Minerals and Materials Technology

Lala Sukla · Council of Scientific and Industrial Research (CSIR), New Delhi

You can refer the paper Advances in high-temperature heap leaching of refractory copper sulphide ores,SW Robertson, PJ van Staden, A Seyedbagheri,jour... [more]

You can refer the paper Advances in high-temperature heap leaching of refractory copper sulphide ores,SW Robertson, PJ van Staden, A Seyedbagheri,journal of African instutute.min metall,vol-112,no-12,2012

http://www.saimm.co.za/Journal/v112n12p1045a.txt ×

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Question:
Open What are the major reasons for nutrient removal in a sequential batch reactor?
An operation of SBR is fill aeration settling decanting. As no proper anoxic conditions with required COD will be available at the time of settling, t... [more]

An operation of SBR is fill aeration settling decanting. As no proper anoxic conditions with required COD will be available at the time of settling, then how does nitrogen removal happen effectively? Is the whole reduction due to endogeneous denitrification? In the case of phosphorus, no anaerobic reactor or condition was provided in standard SBR, so P removal was also unclear.

By Sandip Magdum · Amity University

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16 From the textile industrial effluent, what are the methods available to clear the color of the water and make it potable?
By Narendrakumar Gopakumaran · Sathyabama University

Edward Calt · Integrated Biochem

There are two technologies both based on ion resin exchange. Dow chemical makes the material. A new technology from Prime Separations allows the ext... [more]

There are two technologies both based on ion resin exchange. Dow chemical makes the material. A new technology from Prime Separations allows the extraction of specified materials in a mixed fluid environment. Prime Separations website is http://www.primeseparations.com/.

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1 Can anyone suggest a method to determine the molecular weight of partially pure chromate reductase?
By Manikant Tripathi · Dr. Ram Manohar Lohia Avadh University

Following
Question:
Open How can a biochemist explore his/her research in coordination with vast modern area of 'Biotechnology'?
Biochemistry versus Biotechnology

Biochemistry versus Biotechnology

By Sanjay Mishra · IFTM University

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1 Any idea about what the enzyme Naphthol-AS-BI-phosphohydrolase does? (API-ZYM Slot12)
By Ebru Dulekgurgen · Istanbul Technical University

Marco Bocola · Rheinisch-Westfälische Technische Hochschule Aachen

Dear Ebru, Naphthol-AS-BI-phosphate is a standard phosphatase substrate, you may search BRENDA database for more information on the EC class 3.1.3.. ... [more]

Dear Ebru, Naphthol-AS-BI-phosphate is a standard phosphatase substrate, you may search BRENDA database for more information on the EC class 3.1.3.. http://www.brenda-enzymes.org/index.php4 Marco

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Question:
Open What are the future prospects of microalgae-based bioprocesses in terms of environmental health?
Microalgae based bioprocesses are gaining wide interest for production of biofuels and other high value products. Based on environmental health (in t... [more]

Microalgae based bioprocesses are gaining wide interest for production of biofuels and other high value products. Based on environmental health (in terms of reduction of GHGs), energy investments and other important considerations, what will be future scope of algae-based bioprocesses?

By GS Dhillon · University of Alberta

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6 Can anyone please tell me, how to detect or find any unknown compounds (like organics, carbohydrates or some enzymes secreted by algae.) present in water samples?
By Ramesh Kakarla · Kyung Hee University

Amir Haider · University of Karachi

Dear Mr.Kakarla, My suggestion is 1st extraction is done because there are not only few compounds in algae there are many compounds in it, 2nd identif... [more]

Dear Mr.Kakarla, My suggestion is 1st extraction is done because there are not only few compounds in algae there are many compounds in it, 2nd identification is done. Research techniques you required for identification like NMR, MASS etc. 1. You will get molecular mass through Mass spectroscopy. 2. You will get proton number (no. of Hydrogen) NMR spectroscopy. 3. You will get C, H, N, S numbers. With the help of these you will suggest a molecular structure of your new compounds (if any unknown compound is there).Otherwise if you find the molecular weight of any known and expected compound through Mass spectroscopy, you will not perform any other technique for further study.

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11 Biodegradation of dyes
By Sahil Shah · Gujarat Technological University

Kamaraj Murugesan · Karpagam University

Hi.. My small suggestion is based on your interest you can try with bacteria, fungi, etc..based on initial screening first you find the better organis... [more]

Hi.. My small suggestion is based on your interest you can try with bacteria, fungi, etc..based on initial screening first you find the better organism for degradation..even you can profile the intermediates produced during degradation.. You can also use some cellline work to check the toxicity of the original dye and degraded product..with this you can depend well that the degraded products are less/no toxic when compare with original dye.. Several articles available..but try to follow some standard articles rather than normal journals

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7 How to maintain Hoagland Nutrient Solution(normally the pH is 5.6) at pH 3.0, 7.0 and 11.0?
By Ashirbad Mohanty · Helmholtz-Zentrum für Umweltforschung

Oliver Berkowitz · University of Western Australia

Plants will assimilate the phosphate, so buffer capacity will decrease depending on how much is left in the nutrient solution over time. You can use b... [more]

Plants will assimilate the phosphate, so buffer capacity will decrease depending on how much is left in the nutrient solution over time. You can use buffers such as MES, MOPS, HEPES etc., have a look at standard buffer charts. These will make it more expensive of course.

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About Environmental Biotechnology

The optimal use of nature, in the form of plants, animals, bacteria, fungi and algae, to produce renewable energy, food and nutrients in a synergistic integrated cycle of profit making processes where the waste of each process becomes the feedstock for another process.

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