Drug Delivery

Drug Delivery

  • Sasi Yarragudi added an answer:
    Is there any method to identify/quantify dextrans (Non Conjugated) such as UV/ HPLC etc..?

    Dextrans

    Analytical technqiues for dextrans

    Sasi Yarragudi · University of Otago

    I read literature on the use of ELSD detector with HPLC  in determination of dextrans, i am gonna try this and will update if this works

  • Ndidi C. Ngwuluka added an answer:
    Contributors needed for the research topic: Natural Polymers and their drug delivery applications - are you interested?
    We are hosting a Frontiers research topic "Natural Polymers and their drug delivery applications." This research topic focuses on the processes and applications of natural polymers in drug delivery. For more details, please check the link below

    http://www.frontiersin.org/Pharmaceutical_Medicine_and_Outcomes_Research/researchtopics/Natural_Polymers_and_their_dru/2043

    Do you want to contribute? You can indicate by sending me a message here or through the Frontiers site as you peruse the content of the link above.
    Ndidi C. Ngwuluka · University of Jos

    Hello Karel,

    Its been a while. Unfortunately, the deadline is past. That is the reason the link is not working. 

  • Jagadevappa S Patil added an answer:
    Which procedure is used to encapsulate hydrophobic anticancer drugs into peptide dendrimers?

    Methods of encapsulating hydrophobic Anticancer agents into peptide dendrimers

    Jagadevappa S Patil · University of Mumbai affiliated College.

    You can follow thin layer method of encapsulation using rotary flash evaporator, followed by lyophillization of colloidal suspension.

  • Rajesh Omtri added an answer:
    Can I get help with liposome radiolabeling methods?

    Hi everybody,

    I'm planning some in vivo work with my liposomal drug delivery devices and want to do SPECT imaging for biodistribution and pharmacokinetic characterization of my liposomes and would like some input on methods for radiolabeling of liposomes.

    I'm using 111-In as a radionuclide, and I'm wondering what method for radiolabeling would be the best?

    Either using functionalized PEG-chains with either amine or maleimide and doing a "post-insertion" method similar to the one used for antibody grafting or remote loading the radionuclide, along with a chelator, in the liposomal aqueous compartment?

    Rajesh Omtri · University of Minnesota Twin Cities

    Hi Carols, 

    Here are the papers reported which are similar to what you want to accomplish. 

    http://www.ncbi.nlm.nih.gov/pubmed/24331706

    http://www.ncbi.nlm.nih.gov/pubmed/24735640

  • Hemant Motiram Vishwasrao added an answer:
    How to quantify the release of a hydrophobic drug?
    To quantify the release of a hydrophobic drug in PBS, can the standard curve prepared in a solvent in which the drug is readily soluble be used or the standard curve should be in the same solvent in which the release study is done i.e PBS.?
    Example the drug is highly soluble in ethanol but very low solubility is observed in PBS, so what should be done to quantify, because the standard curve prepared using PBS has some amount of undissolved drug in it.
    Looking forward to some valuable suggestions.
    Hemant Motiram Vishwasrao · University of North Carolina at Chapel Hill

    Have you checked this paper..

    http://www.researchgate.net/profile/Yosra_Elnaggar/publication/279446825_Optimization_and_In-vivo_Appraisal_of_Piperine-loaded_Intranasal_Chitosan_Nanoparticles_as_Brain-Targeted_Dual_Acting_Therapy_in_Alzheimer's_disease/links/5597e10b08ae99aa62c9e343.pdf

    It is there on researchgate itself and uses propylene glycol in the dissolution medium.

  • Gandhidas Sonajirao Lavekar added an answer:
    How to improve pulmonary distribution of most systemically administered drugs?

    i want to improve pulmonary distribution of drug what are current strategies to enhance it?

    Gandhidas Sonajirao Lavekar · Central Council for Research in Ayurveda & Siddha

    Mr Giram, you can give even solid drug or liquid drugs if you use the herbs which are excreted through respiratory route like Garlic and Adhtoda vasaka having mucolytic and expect oratory action; if interested may suggest more but decide your strategy.

  • Çağla Çibuk added an answer:
    What is the reason that the release does not continue? (controlled drug release, HPMC matrix, cefazolin)

    I am working on drug delivery behaviour of cefazolin by using HPMC as a matrix. In vitro release of cefazolin from HPMC carried out at 37 C in SBF pH 7.4. 55% of the loaded drug was released within 6 hours and after that we did not observed any release. We did not explain this peculiar behaviour.
    We think that either cefazolin are trapped within the HPMC matrix or it degrades and we can not observe the change in concentration.

    Çağla Çibuk · Yildiz Technical University

    Thanks to all of you for your answers, I will consider your thoughts.

  • Kaushik Valambhia added an answer:
    Can anyone suggest how to mask the taste of lornoxicam in oral film strips?

    I am working Lornoxicam Mouth dissolving oral film strips. I am tried to bitter taste mask of lornoxicam. Can anyone suggest me how to taste mask of lornoxicam? I am trying with lots of Ion exchange resins as well as Aminoalkyl methacrylate
    copolymer but till it no bitter taste mask.

    Can anyone suggest the sources of lornoxicam?

    Kaushik Valambhia · Muhimbili University of Health and Allied Sciences

    Try coating the oral film in LACTOSE you will definatly see improvement in the intensity  of the bitter taste of the API.

  • Daniel Silva added an answer:
    What is the best way to transfer suspended nanoparticles from aqueous solution to THF?

    I am looking to transfer nanoparticles from a water-based suspension to THF. I have attempted dialysis into THF with the only dialysis membrane type readily available in my lab (mixed cellulose), but the dialysis membrane did not take well to it. I am wondering if there is a type of membrane you would recommend I purchase for this diaylsis, or if there is a better technique for the transfer altogether? 

    They are rather simple organosilica nanoparticles, which are ~ 64.04 nm in diameter.

    Thank you very much for your time and help!

    Daniel Silva · University of Reading

    Hi guys,

    Thanks so much for your help and ideas. I'm quite new to this site but it's already showing to be an amazing resource. 

    I wish I could say I succeeded in getting the nanoparticles into THF, but after trying each of techniques mentioned (except for dialysis with a suitable membrane, because £££) I had no choice but to change tactics and carry the reaction out in DMSO instead. I don't believe they are stable in such a harsh solvent.

    Many thanks again for your generous support.

  • Imran Khan added an answer:
    Which is the best method for encapsulating hydrophobic flavonoidal drugs into central core of a peptide dendrimer

    I am planning for a research on peptide dendrimers and their drug delivery properties of natural flavonoidal drugs. So I need a best method which can load these drugs into peptide dendrimers. Please help me out with this.

    Thank you

    Imran Khan · CAPITA

    You may try out proliposoma technique for the same.

    Spray dryer technique wherein your hydrophobic flavonoidal drug would get centrally encapsulated in peptide dendrimer.

    Very useful...

    Best regards,

    Imran Khan

  • Kazim Emre Karasahin added an answer:
    Are there any studies which have used the mother as the mode of drug delivery via breast milk?

    Are there any studies which have used the mother as the mode of drug delivery via breast milk?

    How would one go about to determine the amount of drug to administer to the mother to reach the litter?

    Krishna et al. 2013 provide a review where they present a formula to calculate human inflant exposure to drug:

    The maternal plasma concentration of drug (Cmaternal), area under the concentration-time curves (AUC) of the drug in maternal milk and plasma (M/P AUC) ratio and the volume of milk ingested by the infant (Vinfant):

    Dose of infant (mg/kg/day) = Cmaternal (mg/L) x M/PAUC x Vinfant (L/kg/day)

    Is this model valid to use in a murine system?

    Moreover, is there a model or formula to calculate how much drug would the mother need to ingest (systemic administration through first pass metabolism) to achieve the target dose for an infant?

    Reviews, original research papers as references or any interjections/insights are much appreciated.

    Kazim Emre Karasahin · Gulhane Military Medical Academy

    This is really interesting, however there would be too many variables that could affect the transfer, and you can not be sure how much drug is delivered.  If there is more than one offspring, one cannot calculate or regulate the amount of milk that each offspring gets from the mother either. 

    I also would think that since only a minor fraction of drug is deposited in the milk, the amount of the drug given to mother for desired effect on the offspring could be detrimental or poisonous on the mother.

    Good luck

  • Sudhir Kumar added an answer:
    What is a 25 wt. % solution of tetramethylammonium hydroxide in methanol mean?
    What does wt% mean?
    Sudhir Kumar · Jawaharlal Nehru University

    I agree with Peter Sobolewski reply. Similar problem you can see here

    http://dl.clackamas.edu/ch105-04/weight.htm

  • Tarik Eljaddi added an answer:
    How to differentiate between “transparent micro emulsion” and “transparent solution”?
    Can UV spectrophotometry be used?
    Tarik Eljaddi · Faculté des Sciences Ain Chock - Casablanca

    May be you can have an idea about the nature of your solution by to find contact angle for determining the hydrophobic property   of your solution, and also you can use a microscope for have a difference .  

  • Olivia Ho added an answer:
    What's this sticking around the Coacervates?

    Using 2% w/v Gelatin A and Gum acacia, 0.5gm crystalline drug and 1ml 10% tween 80.h20

    So I adjust to ph to 3.8 and subjecting it to ice bath to reduce the temperature to 15C.

    Repeat experiment twice:

    I check the coacervate under microscope and

    1) Replicate 1: I see spicky-like stuff sticking around it, and some floating around the solution.
    2) Replicate 2: No spicky-like stuff. Surrounding clear


    = Spicky stuff only appear after continuous agitation at 15C
    I am guessing spicky-like stuff= probably gelatin+arabic. But why is it appearing so?

    Is it because its been cool down too fast, as compare to replicate 2?

    Olivia Ho · Monash University (Malaysia)

    I use 0.5N and 0.1N HCl..
    Is definitely not my crystalline drug, as I repeated the same procedure with canola oil (instead of crystalline drug) and the same thing happens after 15C.

  • Jawadul Misir added an answer:
    In measuring of particle size of Drug Delivery Systems by DLS, which calculatioin (number, volume, or intensity) would be more applicable?
    As you know in measuring particle size by DLS method, three kind of particle size distribution measurements could be reported: number, volume, and intensity.
    Each of them could be applicable in different filed of science. for example in fluid mechanic particle size measurement based on volume is more useful than two other method. In drug delivery systems (DDS), in colloidal state particle size and zeta potential are important, but which of these three method is more useful from DDS viewpoint?
    Jawadul Misir · Shahjalal University of Science and Technology

    In the DLS particle size distribution by volume graph  shows like 15.5 % at 18.5 d. nm or 19 % at 18 d nm. what that represent actually?

  • Kerem Karakuş added an answer:
    Can drug delivery efficiency be improved by pressure?

    We would like to enhance diffusion or delivery of polymeric nano particles/liposomes on a tissue culture model.

    Is there any experienced expertise or literatures regarding external pressure-mediated delivery enhancement?

    Thanks !

    Kerem Karakuş

    Dear Nashwan,

    My assumption is about the micro and nano-gels which will be used in delivery through the blood. The US will definitely disrupt the naive structure of micelles. As you are very well aware of it  they are self-assembled structures which are mostly depend on the inter-molecular forces and entanglement so it would not be surprise that the drugs will set free when the structure keeping the molecules destroyed. As the effect of the US, the pressure and temperature fluctuations will definitely effect the stability of the  micelles.

    When we return to the  adoption of the drug molecules in the gel the Fick's law will not be enough to estimate the behavior in there since it is related diffusion.  The interaction between the drug molecules and the inner walls of the gels should be taken into account. Just like in the case of drug entrapment with micelles.   

    When it comes to the reference studies, unfortunately, i do not have the access to the databases till i left my PhD.  Since i did not study on this effects, i do not have the archive on such issues. But ı have experienced both on micelles and applications of the US. My comments are sourced from the experiences and theoretical information on the basics. But i can surely find the related articles which will explain the effects of the pressure on the loading of the drug molecules to the gels. Or at least there are lots of studies on the effect of the pressure on the adsorption. 

    Best Regards. 

  • Christian Cibert added an answer:
    Is it possible to compare anticancer activity of raw-Curcumin and prepared pure curcumin nanoparticles ?

    recently i prepared nanoparticles of curcumin without any stabilizer, carrier and encapsulating agent. it was pure nanoparticles of curcumin only. i went through literature to know the precedure to compare raw and nano-curcumin anticancer activity but i never found such type of literature. however, there was many papers in which they encapsulated in polymers or stabilizer, or they attached curcumin nanoparticles to different carriers metal nanoparticles, polymers. so i want to know whether it is possible or not ? if noy why ? if yes then what is the procedure for MTT assay. any literture is there then share link with me.?

    regards

    Raj Kumar

    Christian Cibert · Pierre and Marie Curie University - Paris 6

    Then, you must define your control the most precisely possible.

    Regards

  • Ahmed Agiba added an answer:
    Can anyone suggest the excipients for microemulsion with topical drug delivery?

    Can anybody suggest the excipients for Microemulsion with topical drug delivery?

    Ahmed Agiba · Ain Shams University

    I usually use these formulas:-

    Formula I

    Cremophor RH 40
    Anise oil
    Propylene glycol

    Formula II

    Cremophor RH 40
    Fennel oil
    Propylene glycol

  • Jeffrey J Weimer added an answer:
    How can I determine the n exponent of korsmeyer peppas?

    If I have an equation of korsmeyer chart ( log cumulative %drug release and log time) is y = 5.1 x + 3.2 and R2 = 0.8038

    how can determine the n exponent to determine the mechanism of release ?

    Jeffrey J Weimer · University of Alabama in Huntsville

    "You can take two points of time with their respective Mt to complete the equation and isolate the k in each."

    Two points may give the slope and intercept of a line or solve two equations for two unknowns. It is however a sloppy approach to take in this case. The suggested method should be used only to obtain a first guess. The rigorous approach for a publication-quality report is to use non-linear regression fitting methods to get the parameters and their uncertainties.

    --
    We have inexpensive (free), intuitive, user-friendly desktop computational applications today that can perform rigorous data analysis and return fitting values with their confidence limits in the blink of an eye. Why do folks still insist on doing data analysis as though all they have with them is an abacas or hard-copy graph paper?

  • Andrey Drozdov added an answer:
    Is there any interaction ( e.g chemical, steric interaction) between Paclitaxel and Iron oxide?

    What kind of interaction between Paclitaxel and Iron Oxide at pH 7.4, 37 Celsius degree and 1 atm? 

    Andrey Drozdov · ITMO University

    It seems that Paclitaxel is very hydrophobic and very poorly soluble in water. I don't think it can stabilize nanoparticles in aquaous media.

  • Manali Dwarakanath added an answer:
    Does anyone have access to the paper "Artificial Viruses: a nanotechnological approach to gene delivery"?
    Artificial viruses: a nanotechnological approach to gene delivery; Enrico Mastrobattista; Nature Reviews Drug Discovery 5, 115-121, 2006

    http://www.nature.com/nrd/journal/v5/n2/abs/nrd1960.html
    Manali Dwarakanath · Cell Data Sciences Inc.

    Hi! Could you please share the pdf with me? The above link doesn't seem to work.

  • Christopher Jobdevairakkam added an answer:
    Can anyone suggest me the stability parameter of Mebendazole in the stomach?

    I am looking for the kinetic parameters of the mebendazole molecules  into different part of the GI track. is mebendazole stable in stomach?

    Christopher Jobdevairakkam

    Mebendazole belongs to N-carbamate functional Me group. Under acidic condition, carbamates are not stable and tends to degrade to the amine function, losing the carbamate group.

  • Guogang Ren added an answer:
    I would like to conjugate FTY720 with Graphene Oxide for drug delivery purpose, can someone suggest an idea?

    Because of the single benzene on FTY720, it would not pi-pi interact with the planar surface of GO. Although there is NH group on FTY720, we can't do EDC coupling with carboxylic acid of GO due to the needs of those NH groups during drug mechanism. I need to come up with novel idea that allows no disturb of the molecular structure yet a stable bonding. Any suggestions? Is it not possible?

    Thanks for your attention and help~ 

    Daniel

    Guogang Ren · University of Hertfordshire

    Or using so-called bio-derivative chemicals.

  • Katerina Vávrová added an answer:
    Is it possible that the transdermal drug permeation from a polymer alone is better than polymers with penetration enhancers?

    I make a research on transdermal drug release , and i have some foreign release behavior that the drug permeation parameters are better than the permeation with adding different type of penetration enhancers

    Is it true and possible?

    Katerina Vávrová · Charles University in Prague

    Dear Mai,

    yes, this is possible, we have seen it as well (enhancers that worked well for certain drug/formulation did not show any effect for other drugs/formulations). Apart from their direct effect on the stratum corneum, enhancers can modify the properties of the drug or formulation, drug release from formulation, partitioning into SC, etc. I suggest trying enhancer from different chemical group (such as amphiphilic enhancer if you tried solvent-like enhancers before).

    Best wishes,

    Kate

  • Rajeswari Raja added an answer:
    If we use polymers to deliver peptide-based drug, how would it react in our stomach?

    If we use polymers to deliver peptide-based drug, how would it react in our stomach? how is the process of that drug to get absorbed through the small intestine? when will the molecules get released out of the polymer? i'm a student and just started studying about drug, i read some papers but still doesn't make sense to me

    Rajeswari Raja · arya colege of pharmacy

    Hi

    naked drugs do not know where to target. But they specifically have some receptors to bind with. That makes the difference in their bioavailability and efficacy. But they can be surely targeted with their specific properties they have like, ionization constant,  lymphatic transport, permeability and affinity etc towards the specific receptors by designing them in the form of  self emulsifying delivery systems, etc

  • Sree Gayathri asked a question:
    How do I analyze the mechanism of drug release-rate kinetics? Is it first order or zero order kinetic by looking at the drug release results?

    How do I analyze the mechanism of drug release-rate kinetics? Is it first order or zero order kinetic by looking at the drug release results?

  • David M Benjamin added an answer:
    Could anyone suggest a detailed procedure to measure the permeability of synthesized drugs using everted intestine technique?

    Could anyone suggest a detailed procedure to measure the permeability of synthesized drugs using everted intestine technique?

    David M Benjamin · Northeastern University

    Look up the work of Stanley A Kaplan who did these studies at Hoffmann-La Roce where we worked together in the 1970s

  • Adel Marzban added an answer:
    Can anyone help with drug delivery and targeting to liver specifications?

    What is the specification of drugs carriers for targeting and delivery to Liver, and whats the qualified surface charge and particle sizes for targeting liver and binding with kupffer cells?

    Adel Marzban · Islamic Azad University Tehran North Branch

    Dear Sunil Mahor and Riyaz Ali Osmani thanks alot for your answers,

    please give me any data you think it can help me, in Hepatic Drug Delivery

  • Prakash Naga Pillai added an answer:
    Do I need to take anti-fungal tests for anti-bacterial drug delivery?

    i have prepared polymeric hydrogel in that i like to deliver the anti bacterial drug since i have doubt on do i want to take  both anti fungal and anti bacterial test or alone enough anti bacterial test only

    Prakash Naga Pillai · Thangavelu Group of Colleges

    Dear madam,

    For any polymeric combination, should be present both test for anti fungai and anti bacterial test.

    Thanking you,

    N. Prakash

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