- Misty Wakler added an answer:6What irradiation dose can be applied to selectively deplete/reduce BM dells but not peripheral immune cells in mice?
I would like to reduce the numbers of BM cells through irradiation and reconstitute with new BM cells, or not. The goal would be to study whether peripheral immune cell precursors are able to regenerate the hematopoetic system without BM-precursors.
Regeneration by plasticity or transdifferentiation. Cell-cell fusion. BM stromal cell: progression checkpoints influenced by fetal liver and adult bone marrow stromal cell macroenvironment.Following
- Denise Priolli added an answer:8Does anybody have any experience with 786-O(RCC) induced xenograft nude mice models?Any suggestions about the optimum cell no. for inoculation as I had inoculated 786-O (5 *10 ^ 6 cells/animal, Subcutaneously in flank region) in CD1 nude mice. After one week I observed tumorous swellings up to 100 mm3 at the site of inoculation and then it disappeared after the 2nd week. What could be the reason?
Dear Milind. I have the same problem with xenograft 786-0 tumor cells in nude model. What do you solve it?Following
- 2Are DMBA-induced tumors folate positive or negative receptors?
subcutaneous injection of 7,12-Dimethylbenz(a)anthracene (DMBA) to the mice
Dear Dr. Llya Tsyrlov
I am very appreciated and thankful of you for the great comment about DMBA-induced tumors. I would be more thankful of you if you send me any related references for FRα expression on DMBA-induced tumors or any simple method to detect its expression on the DMBA-induced tumors.
- Rozita Nasiri asked a question:OpenWhich type of cells establish cervical cancer or folate positive receptor cancer in normal balb/c mice?
I am going to use normal balb/c mice to establish folate positive receptors cancerous mice. As 90% of cervical cancer are folate positive receptors, I was thinking that it consider as a good model. Any other suggestion would be appreciated.Following
- 6How can I induce immune suppression in normal laboratory balb/c mice using drugs or other simple methods?
Does anybody know that how I can induce immune suppression in balb/c mice using drugs or any other simple method? Needless to say I cannot use nude or SCID mice in our conventional animal lab. On the other hand, I want to establish cancerous mice using human cancer injection after I get immunosuppressive balb/c mice. Thanks in advance.
Rally appreciated and thankful for the great and helpful comment. I will follow your method.Following
- Gudrun Lang added an answer:4What is the protocol for wax infiltration of mouse kidney?
we use an automatic infiltrator in our lab which can be programmed to change alcohols at set times and can let the tissue sit in wax for a set time. does anyone know the optimum times for alcohol washes and wax incubation?
i've done liver samples before, for which i've used 1 hour each in 100%-30% alcohols, 1 hr each in citrosolv (twice), and half an hour each in wax (twice).
i want to know if there is a different timing system for kidneys.
As long as Ganesh works with an infiltration-instrument, the times with 1-2 hours will be sufficient.Following
- Geraldo A Passos added an answer:2How can I choose mice mammary tumors for microarray?I want to isolate mice tumors from both MMTV-PyMT control mice and MMTV-PyMT mice with a special gene knock-out. The question is how to choose the tumors for microarray? Should the tumors be age matching or size matching or histological matching? For one sample, should be just one tumor or merging like 3 biggest tumors per mice? Or should merge different mice in one sample?
For further reading about transcriptome analysis (microarrays and RNA-Seq) I suggest a recent publication from our group:
Assis AF et al (2014) Chapter 1: What is the transcriptome and how it is evaluated ? In Transcriptomics in Health and Disease. Passos GA (Editor). Springer International. 374 pp.Following
- Anusha Mishra added an answer:3Can we use female rat instead of male for lung cancer studies?
Single intra peritoneal dose of benzo(a)pyrene will be used to induce lung cancer.
There are so many differences that researchers are starting to discover about gender differences that excluding either sex would probably present a big confound. As already mentioned by the above replies, it is best to include both sexes in doing experiments. This will ensure that the effects observed are true for both sexes (especially for clinically transferable research such as cancer) and if a pattern of difference between the sexes emerge, you might discover something important and ground-breaking. Imagine if a protocol that induces lung cancer in males doesn't do so in females, that would be a very interesting thing to study in itself!Following
- Zul Gerel added an answer:4Which lung and liver cancer cell lines do you find grow best in nude mice?
We have synthesized liposomes for siRNA delivery and aims to examine in vitro and in vivo. Which lung and liver cancer cell lines do you find grow best in nude mice?
- Rohitash Jamwal added an answer:3Is the ORAC test reliable or with any biological relevance to measure antioxidants capacities in plasma of mice ?
Rodent model of colon cancer. Is the ORAC test reliable or with any biological relevance to measure antioxidants capacities in plasma of mice ?
If you want to get rid of the proteins, use protein filters and concentrate your sample. Lyophilise the residual water which will contain the hydrophilic compounds which are free and not protein bound. Use this to get an estimate of the anti-oxidant capacity. After all protein bound analytes are rarely active.Following
- 10Does anybody know about cell line injections in immunocompetent balb/c mice?
I am developing two different cancer models in immunocompetent balb/c mice by subcutaneusly injection of Hela and SK-BR-3 cell lines. Appreciate any suggestions.Is there any option to generate tumor in an immunocompetent balb/c mice?. Can we make them immonodeficient? If there is no possibility to use immunocompetent balb/c mice then which type of mice is suggested?
Thanks a lot for this great comment.Following
- Prijovic Mirko Zeljko added an answer:4Why did balb/c mice lost their hair one month after subcutaneusly injection of Hela cancer cell?
The immunocompetent balb/c mice were injected by Hela cell lines. They lost their hair one month after injection, but there is no cancer bumping yet.
Stress itself may cause hair loss at mice. Contamination with hair follicle mites is another reason. May not be related to cells injected.Following
- Hem Chandra Jha added an answer:3Any advice on the quantification of mice tumors?
How to measure tumor size in corel pictures. Suppose I have mice pictures with tumors and these tumors are separated from body part. The problem in the quantification of tumor size as by visual and corel measurement not looks correct. Someone have an idea how to quantify these tumors? Thanks in advance.
Thank you Saleh and Lakshman Ji...Following
- Khalid El BAIRI added an answer:10Why do we prefer male rats instead of female for cancer studies?
Ca we use female rats for chemical induced lung cancer.?
It depends on the type of cancerFollowing
- Victoria E. Palau added an answer:1Is there any alternative for propylene glycol (Propanediol) in Ehlrich Ascites carcinoma models?
Kindly suggest the alternate for Propylene glycol which is commonly used as a vehicle control in the experiments (Genotoxicity and antitumor studies) pertaining to EAC models..and also explain why propylene glycol is the most preferred vehicle control for comparison studies done using chemotherapeutic agents.
I have attached an article for better understanding..
Not really, it depends on your agent and its solubility properties (polarity). This will determine which dissolution vehicle you will use (ethanol, acetone, DMSO, etc.) It is important to prepare a stock solution so you can keep the concentration of the dissolution vehicle below toxic levels.Following
- Mohd.Ahmar Rauf added an answer:7How can I Induce breast cancer in model mice animals?
I have been working on development of delivery system for breast cancer and had to check my formulations for the same
Thanks Robert clarke sir ,lucia mamFollowing
- Anuj Singh added an answer:7Which colorectal cancer forms faster tumors in athymic mice? Caco-2 or HT-29?
I want to grow tumors using either Caco-2 or HT-29 in male balb/c mice. Which of these cell lines would be recommended in terms of ease of maintenance of the cell line in culture as well as time taken to grow tumors in vivo? Also which of the two cell lines is considered more aggressive?
- Kristopher R Bosse added an answer:3Has anyone used an IPTG inducible shRNA system in vivo (mammary fat pad tumors)?
My pilot experiment showed no reduction in the target protein in the excised tumors. I would love some feedback on dose and administration of the IPTG agent! Thanks!
curious if you got an answer to this as I had the same question?Following
- Carlos Eduardo Fonseca-Alves added an answer:6Is it possible to generate cell line from frozen tissue?
I'm working with prostate cancer from dogs and is not a very common disease. I have some frozen tumor and I have seen few protocols to generate cell culture from frozen tissue. Is it really possible to generate cell line from frozen tissue?How do you establish a cell line from the frozen tissue sample?
I would like to generate culture with epithelial cells. Neoplastic epithelial cells. I had not thought about alterations in cell morphology/metabolism. It 's a good point. Thank you so much for the advice. I will think about it and maybe I will wait for a fresh tissue.Following
- Ichun Chen added an answer:6Are there any murine models breast cancer recurrence ?
Does anyone know or have a breast cancer murine model to study recurrence? I have checked the literature, but did not find relavant references. I am not sure whether it was because there's no good murine model for this or I did not use good keywords.
Thanks for your kind help
Thanks for all of your recommendations. In addition to transgenic mice, I knew that 4T1 might be a good model for this issue. However, does any one have an estimated idea that how long does it take for 4T1 cells to develop recurrence after primary tumor resection? In addition, to what size should we resect it? Once it is visible? We are working on B6 mice basically, so 4T1 might be a good fit. However, it is growing really fast that our intervention might hardly have a chance to see the difference...Following
- Rubén Fernández- Rodríguez added an answer:1Does anyone know of a cancer cell line which could grow in 129Sv/Ev mice?
My project is focused on a specific genetic knockout mice which I bred into 129Sv/Ev. I did some nice tumor experiment in C57BL/6 and 129Sv/Ev mix background (F1 intercross). However the mice have been bred into 129 for like 9 generations, I found my previous tumor cell (b16 melanoma) not growing very well in these guys. They start off fine, but as time goes by, 2 weeks plus they are still really small. I gave them DC vaccines though, but based on my previous experiment, the tumors should still grow even if the mice receive vaccines.
B16 cells have a C57 background and only develope tumors on animals of the same background, in this case C57. As you crossbreed your animals with the 129 mice you start losing the background and the more you breed the more you lose. And so, you will eventually not be capable of growing B16 tumors on your mice.Following
- Eleonora Petrucci added an answer:5Any advice on intraperitoneal injection of matrigel?
Our lab uses frequently uses matrigel for intraperitoneal injection of ovarian/endometrial tumour cells. However, there is no literature I can find that describes the use of matrigel with IP injections, and it is only recommended for orthotopic and subcutaneous injections.
Intraperitoneal injection is more like a metastatic model and so it seems as though the formation of a matrigel plug is counter-intuitive.
Should I stick to just using PBS? Or should it not really matter?
Thank you for all your answers so far! I would like to add that with gynecological cancers, direct spread to the peritoneal cavity is very common; metastasis via the blood stream not so much. That's why we inject tumour cells intraperitoneally.Following
- Anuj Singh added an answer:8How do I decide which cancer mouse model to use?
We have developed some molecules against a carcinogenic Protein. Overexpression of this carcinogenic Protein is associated with diverse types of cancer. I want to develop mice models to test these new anticancer molecules?
What would be the suitable model for this?
I would like to suggest that you first decide the carcinogenic Protein overexpression , whether it is related to solid or liquid tumor, decide the human cell lines. Perform a cytotoxic assay (in vitro) in a panel of related cell line. Choose the best effect. Mean while you can perform PK study of your molecule in normal mice and see it its bioavailability. Now if all tests are okay, see whether the xenograft of the best in vitro selected cell line is available or not. Depending on the types of tumor you can select either SCID or nude mice.
In my experience SCID mice are best for liquid tumor and Nude mice are for solid tumor.
In nude mice female are best because male mice will fight among themselves after grouping.
Good Luck !
- Syarifah Nur Syed Abdul Rahman added an answer:1Can we develop breast cancer cells (MCF-7) in swiss albino mice?Due to lack of nude mice we are interested to develop breast cancer in normal mice. does any one have the idea?Following
- Alastair D Lamb added an answer:1How well do orthotopic xenografts of CAL51 grow in NOD/SCID mice?
I want to measure tumor growth in NOD/SCID for CAL51 implanted in mammary fatpad. How long it takes for tumors to develop and reach 1000 mm3?
My experience is with prostate cancer xenografts in NSG mice (we find these much better for xenos and PDXs) and they generally run for between 6 weeks and 4 months depending on how aggressive the cell line. I don't know CAL51 but I understand that our breast cancer colleagues run fairly similar length experiments for MCF7 cells. Also, we would never grow to 1000mm3 as that would be much too big for most UK project licenses. We usually go for 100mm3 (so about 4x4x5mm)
- Go J Yoshida added an answer:1How can I establish GIST (human gastrointestinal stromal tumour) exograft model?
I`m now doing some research about GIST(human gastrointestinal stromal tumour), and there is a big problem that I have failed to establish GIST exograft animal model. 1×107 GIST T1 cell line were given hypodermic injection on nude mouse, after 10 days, tumor was failed to growth. Some one who have successful established this exograft model could give me advice, thanks.
You have to know the importance of where tumor cells are transplanted. GIST occurs from Interstitial cells of Cajal (ICC) with active mutation of c-KIT, the receptor of SCF. That is why the hypodermic injection is very artificial and difficult for GIST T1 cell line to form xenograft tumor tissue. Of course, so many kinds of tumor cell lines are able to form xenograft tumor under the skin of nude mice, but it has been widely accepted that the orthotopical transplantation is much better to reflect the tissue-dependent characteristics of each tumor. Why not try to transplant G1 cells into the gastric or intestinal mucosa?Following
- Maninder Kour added an answer:4How do you induce mammary tumors in wistar rats?
Do we get results? If yes then in how many days does the tumor develops?
Thank u so much for the answers.Following
- Yoann Pageaud added an answer:5Do you know a good marker of endothelial cells in the mouse model tumor CT26 ?
I'm looking for a specific marker of the endothelial cells in a murin mouse model for CT26 colorectal tumors.
I already check some interesting antibodies : anti-CD31, anti-CD34, anti-CD105, anti-vWF.
And Isolectin B4 staining.
So now I am looking for anything else that could be as specific as these marker, or more.
And what is your opinion about anti-SMA and anti-vWF ? any other markers to suggest ?
Which marker could be the most relevant for this type of tumor (Murin Colorectal CT26 Tumor) ?
Please for antibodies precise the compagnies names you suggest for providers.
Thank you all for your help !
Thanks a lot Ricardo. I'll look for CDH5, CD144 and avB3.
I keep your article it will be helpful.
- Mengyu Wang added an answer:6How can I establish good 4T1 tumors in-vivo?
I'm growing 4T1 cells in mice. I'm implanting two contralateral tumors per mouse. I'm having a hard time getting the tumors to grow symmetrically. I understand they won't all be perfect, but I'm getting a lot of shapes that are difficult to caliper, or I'll have tumors with small satellite tumors right next to them. With 2 tumors on 1 mouse, its hard to get enough mice to enroll in study.
I'm implanting 1 x 10^5 cells in 100 uL volumes. I draw the cells up into the syringe in an 18 g needle, and I implant the cells using a 25 g needle. I slowly inject the tumors on the flank and slowly remove the syringe. The cells are being implanted using serum free media.
Should I use a smaller gauge (27g) needle to implant? Should I move my implant site up to the shoulder? Should I switch to PBS instead of media?
You can inject 4T1 cells from 5x103 to 1x105 in the up or low fat pad with volume 30ul to 50ul in Balb/c mice. Then you can have lung metastasis and larger spleen ( 3-4 time weight (g) than normal mouse''s spleen.Following
- Go J Yoshida added an answer:1Can we use combination of NMU and DMBA to induce mammary tumors in rats to get better incidence?
MANY AUTHORS HAVE REPORTED DIFFERENT INCIDENCE AND TUMOR LATENCY FOR RAT MAMMARY TUMORS. CAN WE USE BOTH CARCINOGEN AT DIFFERENT TIME INTERVAL TO INDUCE TUMORS FAST WITH BETTER INCIDENCE?
Given that both N-Nitroso-N-methylurea (NMU) and 7,12-Dimethylbenz(a)anthracene (DMBA) belong to mutagen chemicals and contribute to the initiation, some kinds of promotion to selectively expand the clone with mutation are considered to be required. Why not use TPA?Following
About Cancer Animal Models
A forum to address questions regarding cancer animal models.