Biology and related sciences.

• Ezeike Christine

  In vitro assays for wound healing

  I am trying to identify the best in vitro assays for wound healing . Can any one throw in ideas?

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    Luc Benichou replied

    Go to http://www.gredeco.com/gredeco-en.htm They have developed an exclusive method of experimentation which answers your question... They are good friends of mine and I'm currently working on LowGo to http://www.gredeco.com/gredeco-en.htm They have developed an exclusive method of experimentation which answers your question... They are good friends of mine and I'm currently working on Low Level Laser wound healing.... My site (sorry it's in french) concerning my private practice : http://www.phototherapies.com

    5 hours ago
• Yogesh .m

  "Man evolved from an ape"

  What is the evidence and the supporting factors behind this statement? How could one trace the path of mankind's evolutionary history?

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    Javier Alonso replied

    @ Babu G. Ranganathan: "Contrary to popular belief, scientists have never created life in the laboratory": Of course!!! Who said they have? Urey-Miller experiment only produced organic@ Babu G. Ranganathan: "Contrary to popular belief, scientists have never created life in the laboratory": Of course!!! Who said they have? Urey-Miller experiment only produced organic compounds usually present in the metabolic processes of what we call "life", and the compound found in space I mention is one of the basic aminoacids in ADN, guanine if I recall well. What we still don't know is how these simple bricks got combined in an autoreplicating macromolecule, which I think is the first step to start talking about "life". And I doubt we're anywhere near the answer. Some hypoteses have been voiced but nothing even promising by now. "Even if scientists ever do produce life from non-living matter it won't be by chance": Of course. This will happen by means of the Laws of Nature acting through human acts, as I fear that "chance" is a term not to be applied here. Is there something really, truly "Chancy", "Hazard" is really "hazardous"? "so it still wouldn't help support any argument for evolution.": Of course I agree, too. Evolution: a process with life interacting with environment. Life: who really knows? fuzzy definition.

    5 days ago
• Xing Qun Huang

  How phytohormones regulate seed development?

  I am very interested in this issue!

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    Maryia Ivanova replied

    Russian is the collection of reports on the regulation of "REGULATION OF GROWTH, DEVELOPMENT AND PRODUCTIVITY OF PLANTS" (october, 2011). there are many articles in Russian, but there are some inRussian is the collection of reports on the regulation of "REGULATION OF GROWTH, DEVELOPMENT AND PRODUCTIVITY OF PLANTS" (october, 2011). there are many articles in Russian, but there are some in English. maby can help you.

    СБОРНИК_2011.pdf

    Dec 9, 2011
• Leeba Balan

  Im working on medicinal values of plants. Can anyone help me wether alkaloid nature compounds have anti-cancer property?

  Isolating compounds on both alkaloid and flavonoid nature.

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    Ikechuku Igwenyi replied

    Flavonoids have anti-cancer activity but alkaloids may not have. See attachement.

    Alkaloids and Flavonoids.doc

    Dec 5, 2011
• Alexandra Krasilnikova

  Problems related with origin of life! What was the first: DNA or RNA?

  Waiting for your comments, tips, articles!

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• Aditya Parekh

  Online collaborations

  Dear Teachers/students, I am presently engaged in a research project in Calcutta, India, after completing my master course in biochemistry. I am going to address a serious topic here especiallyDear Teachers/students, I am presently engaged in a research project in Calcutta, India, after completing my master course in biochemistry. I am going to address a serious topic here especially to higher ranked professionals and also to students of my category. I understand the dearth of research collaboration all through out the world between the researchers individually or through institutes/universities. If the research works are shared more then I think science will progress at a much much faster rate. Just like if two people share ideas among themselves, the gross idea of each individual increases and thinking of both rises to a higher level and have wider directions. I know there are some important factors like secret of scientific works until published, publications, individual copyright etc needs to be considered while collaboration but isn't these points a bit cheaper in front of greater scientific progress? (giving due respect to existing regulations in scientific research). It's not only just about collaboration relating a particular topic between two laboratories. what I mean to say is that can't the collaboration level rises to a higher level? Can't research or for that matter papers or publications be created by/between researchers sitting far apart from each other but by sharing pieces of individual biological ideas, research works. Isn't the pace of scientific progress slow? perhaps one of the reason may be lack of collaborations between researchers. I think I have made my views pretty clear by now. I do not want the researchers to deviate from their original works but only want much more collaborations between the scientists performing similar research work. and more research being carried out and shared between individuals especially in the world where all can be easily accessible through world wide web, that is internet. why can't a PhD scholar apart from doing his/her own work also contribute to/ collaborate through internet with other members to give rise to new work in similar or related fields? My direct issue is that why can't groups of group members be formed and share their dry lab work or/and wet lab (as per time, conditions and infrastructure permits) together to give rise to new publications and thus contributing very faster to scientific world. Does it always need to be a part/member of same lab? why can't an online laboratory be formed where the data of the experiments of individual researcher may be shared may be in a secret way and there is a guide supervising it and in the same way publications can also be made.? I have written a lot. I want views, discussions and suggestions from scientists as well as students about how this can be conducted efficiently and it's pros and cons. Please write and suggest. High Regards to all Aditya Parekh

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    Roi Paz replied

    We are consortium of scientist from 27 countries running a research in Africa, we looked for a tool that will able us to collaborate online and at the same time keeping part of our work private toWe are consortium of scientist from 27 countries running a research in Africa, we looked for a tool that will able us to collaborate online and at the same time keeping part of our work private to each lab. After a year that we looked and tested such tools we found Sparklix lab notebook and all of the labs we work with liked it. We found it very useful since we can share our experiments very easily with each other, include any type of data in it, create projects and milestone, and in general it make us to work in more organized way. I liked it mainly because it is simple to use and it is free (no space limit). I am strongly recommend you to check this e-Notebook at www.sparklix.com

    Nov 28, 2011

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    Saji George replied

    Shrimathi Ravikumar, I think you got confused by my name. I am very much a male Homo sapiens! Lols!! Its ok About adding starch to solid medium, you have to hydrolyse it and then autoclave andShrimathi Ravikumar, I think you got confused by my name. I am very much a male Homo sapiens! Lols!! Its ok About adding starch to solid medium, you have to hydrolyse it and then autoclave and add. but your agar and other nutrient in the solid media has to be weighed accordingly so that the final concentration reaches to the level what you want.

    Nov 15, 2011
• Priyanka Halder

  How to isolate DNA of a single species population of zooplankton and sequence it?

  Are any of you working in molecular level of plankton?

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    Priyanka Halder replied

    ok thanks a lot Sir. :-) I will let u know soon.

    Nov 9, 2011
• Thirdsak Kajornboon

  I have some problem, I've do 2 method ; Total sugar(phenol-sulfuric) and DNS method (Reducing sugar), I found that the reducing sugar was more than total sugar Why? (I studied about kojic acidI have some problem, I've do 2 method ; Total sugar(phenol-sulfuric) and DNS method (Reducing sugar), I found that the reducing sugar was more than total sugar Why? (I studied about kojic acid production from Aspergillus sp. using cassava starch as substrate)

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  • 
    Saji George replied

    Please prepare your DNS fresh and try again

    Nov 9, 2011

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  • 
    Didier Jambou replied

    Hello Esther, "enzymes are denatured at about 40 degree celsius" Does it depend ofnspecies from which enzyme is originated ? in this case you talk about "our" enzymes (mammalians)...ie Hello Esther, "enzymes are denatured at about 40 degree celsius" Does it depend ofnspecies from which enzyme is originated ? in this case you talk about "our" enzymes (mammalians)...ie thermophilus aquaticus enzymes function at 72°C. Regards Didier

    Oct 31, 2011
• Gloria Galan

  Is it possible to make use of plant extracts to reverse the sex of tilapia?

  Tilapia fingerlings are fed with meals mixed with methyl testosterone hormone to reverse the sex of tilapia from female to male

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    Pralhad Patki replied

    Sex reversal of newly hatched tilapia generally is accomplished via oral administration of 17a-methyltestosterone (MT), which has been incorporated into a starter fish feed at 60 mg MT/kg feed .Sex reversal of newly hatched tilapia generally is accomplished via oral administration of 17a-methyltestosterone (MT), which has been incorporated into a starter fish feed at 60 mg MT/kg feed . Although the use of the 60 mg MT/kg feed dose consistently yields populations comprised of less than 5% females (i.e., > 95% males), this has not been shown to be the optimal dose. Other investigators have reported sex reversal of tilapia at dose rates less than 60 mg MT/kg feed however, results from some of these studies are inconsistent, and it is difficult to separate treatment environment effects. Thus, it is necessary to identify the optimal dose of MT for consistent, successful sex reversal in a variety of treatment environments. Naturally occurring sources of testosterone may be an alternative to using a synthetic androgen, which also is an anabolic steroid, for tilapia sex reversal. Ref- Sex Reversal of Tilapia: 17a-Methyltestosterone Dose Rate Environment and Efficacy of Bull Testes . Interim Work Plan, Honduras, Study 4 Ronald P. Phelps, Leonard L. Lovshin and Bartholomew W. Green Department of Fisheries and Allied Aquacultures Auburn University Auburn, USA

    Oct 28, 2011
• Avinash Gaikwad

  how caprylic acid precipitate non specific protein except Immunoglobulin G (Ig G) from plasma? what is mechanism behind that?

  during antisera production first plasma sent for pepsin digestion and then for caprylic acid precipitation during which caprylic acid precipitate only non specific protein except IgG.

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    Avinash Gaikwad replied

    Thanks for your opinion. regards Avinash

    Oct 23, 2011
• Laxmikanta Acharya

  How to find out salt stress protein from a mangrove plant that never occur in normal mesophytic habitat.

  I am working with a plant which i can not grow hydroponically. how can i find the salt tolerant proteins in the plant

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  • 
    Santosh Kumar replied

    Hello Jagruti Mam, its very nice that you liked my idea. First you have to define the limit to tolerate the salt concentration of your plant of interest. Once you have standardized the stressHello Jagruti Mam, its very nice that you liked my idea. First you have to define the limit to tolerate the salt concentration of your plant of interest. Once you have standardized the stress condition and concentration of your plant, then you need to give stress treatment to your plant by keeping a set of control. You can keep different sets for different time periods. After the stipulated time of your experiment, harvest the molecule (DNA / Protein) from all your experimental sets including control and then do the profiling part. You can do both genomics as well as proteomics from these samples and could come up with some very noble data. For genomics based profiling you can opt for microarray and for proteomics based profiling you need to do 2D gel electrophoresis followed by characterization using MALDI. I hope you would have explained what I was asked.

    Oct 13, 2011
• Gajendra Pal Singh Raghava

  Translational Medicine

  We are organizing international conference of "Open Source for Computer -Aided Translational medicine", we are looking for suggestions for possible topics and titles of sessions. Our overallWe are organizing international conference of "Open Source for Computer -Aided Translational medicine", we are looking for suggestions for possible topics and titles of sessions. Our overall objective is to promote translational medicine from basic to clinical science, we wish to include all topics/steps important for bring medicine against dread diseases, in market. Your suggestion will be useful to improve content of conference. We believe in open source and hope you will share your thoughts with community. Please also suggest speakers for this conference.

  http://crdd.osdd.net/oscat/