Bioimaging

Bioimaging

  • Vladimir Bobic asked a question:
    Does anyone have any experience in imaging human Lamina Splendens?

    The uppermost layer of hyaline articular cartilage is called Lamina Splendens. This film-like layer is seldom seen arthroscopically and various optical and electron microscopic imaging studies have shown conflicting morphology and structure. Considering its location there is no doubt that Lamina Splendens has extremely important articular function as it provides a very low friction lubrication surface and contains lubricant and collagen fibrils which run parallel to the surface of articulation. This surface zone layer is likely to play a key role in maintaining the mechanical response of articular cartilage to load.

  • Ahmed M Alameldin added an answer:
    How do you convert DICOM images to a 3D solid model (iges, step, etc) which needs to be input into Abaqus for further analysis?

    I currently have a set of 500 DICOM image of slides of a rhino's frontal snout and I am specifically interested in its frontal horn for further analysis.

    I have tried a lot of software for the conversion but have had no success yet. It would be great if I could use any free software which does the above conversion directly.

    Ahmed M Alameldin · Al-Azhar University

    Did you find an answer? I have the same problem and I don’t know what to do. 

  • Irais Rivera added an answer:
    Has any institution had issues with UVP imaging products?

    We've had a few issues with our UVP imaging system which seems specific to our camera. I'm just curious if any other institutions have had similar issues with this brand?

    Irais Rivera · Instituto Tecnológico y de Estudios Superiores de Monterrey

    Recently we had problems with the camera USB port, and makes impossible to get images, the waiting time to get the damaged piece is 90 days.  

  • Olivia Harding added an answer:
    Does anyone have experience with 3D reconstruction and volume rendering of confocal z-axiz stacks?

    I'm analysing ER-mitochondria contact sites but I can not find a detailed protocol to obtain 3D reconstructed and surface rendered images with ImageJ.Thank you

    Olivia Harding · National Institutes of Health

    If you have access to Imaris software, it provides very high quality 3D rendering for confocal images. Imaris also allows data analysis based on your 3D rendering parameters. 

  • Jayampathi Basnayake added an answer:
    Is there any software for converting RGB fractional exposures to real wavelengths?

    I am using an IDS camera to identify RGB color ranges on some diseased leaves. It is easy to pick up the fraction of RGB (out of 255 grades) but I need to convert this into real wavelengths. Any ideas or advice would be appreciated.

    Jayampathi Basnayake · Sugar Research Australia

    Thanks  a lot for all your contribution. I found a very interesting paper about how to modify the IDS camera to be able to capture the wavelength from RGB. Please see the attachment. 

  • Jayaramakrishnan Velusamy added an answer:
    What are the instruments and components are need for establish new Transient pump -prob or Transient Absorption Spectroscopy for bioimaging?

    I need clear each and everything about instrumentation setup from basic alignment  to final calibration of pump-prob spectrometer. I need all laser systems functional procedure and what are the safety measures to be taken and consider while in this experimental arrangements? Give some basics to advance ideas to setup this facility. Each and every aspects of ideas will be appreciated and make an excellent setup to be utilized many researchers  for their research works.

    Jayaramakrishnan Velusamy · Centro de Investigaciones en Optica

    Thanks Klier, I would like to use liquid as well as thin film samples for measurement especially indeed to study polymer samples and we have to lunch all system so give me detailed from basic to advance to install and how to work out in ultrafast Transient Absorption Spectroscopy for bioimaging.

  • Yoon-Soo Jang added an answer:
    What's the working principle of micro channel plates (MCP) Photomultiplier tube (PMT)?

    Why it's so special compared to traditional PMT? Why it can't respond to high repetition rate (tens of MHz) and need to be pulse picked to low down the repetition rate (~ tens of kHz)?

    Yoon-Soo Jang · Korea Advanced Institute of Science and Technology

    It takes a time to multiply photon in MCP.

  • Cao Guoyang added an answer:
    What is the refractive index of graphene oxide?

    Refractive index of graphene oxide in thin film.

    Cao Guoyang · Soochow University (PRC)

    Hi!Did you have the detailed answer for your question? Could you share the answer for us? Thank you!

  • M. Ricky Ramadhian added an answer:
    How do you quantify fibrosis in an aorta wall with imageJ?
    I want to quantify fibrosis in an aorta wall with ImageJ but it seem difficult to analyze. I read there is journal to use polarization microscope, but in my laboratory there is no polarization microscope. Can ImageJ make images become polarization microscope?
    M. Ricky Ramadhian · Lampung University
    to adjust threshold,,,
  • Abhijeet Lale added an answer:
    Is there any optical properties difference between Graphene quantum dots and N-doped graphene quantum dots?
    Actually, graphene quantum dots (GQD) show different optical properties. Is there any change in the optical property after doping with nitrogen in the GQD?
    Abhijeet Lale · Indian Institute of Technology Madras
    Yes there is difference between optical properties of undoped GQD and N-doped GQD. The undoped GQD has a very high fluorescence while doped GQD have no detectable photo-luminescence. Please refer to paper by Q Li published in JACS. The following explanation is taken from the same.
    This is consistent with the absence of n→π* , the lowest-energy transitions, in
    the absorption spectra of doped GQD. The n→π* transitions are expected for the N-substituted aromatic heterocycles. The large size of doped GQD explains the diminishing oscillator strengths of the n→π* transitions, because of the decreasing orbital overlap between the increasingly delocalized π* electrons and the nonbonding electrons localized on the nitrogen atoms. In contrast, the lowest-energy transition in undoped GQD is π→π*, which is responsible for the emission and has a less size-dependent oscillator strength.
  • Arunas Ramanavicius added an answer:
    Any suggestions on resolving the "blinking" of quantum dots (CdSe/ZnS) for confocal microscopy?
    I am trying to image the distribution of quantum dots (QDs) in tissue using confocal laser scanning microscopy. I am having a hard time tracking them and it is well known that the blinking of QDs poses a problem in bioimaging. Does anyone have suggestions to resolve this issue?
    Arunas Ramanavicius · Vilnius University
    You can encapsulate them within polymeric layr, one example is attache:
  • Sankalp Verma added an answer:
    How can we prevent photobleaching in fluorescent labeled protein stock solution?
    My protein samples are labeled and are kept in dark at -80. I am afraid they might undergo photobleaching if kept for long.
    Sankalp Verma · Indian Institute of Technology Kanpur
    thanks...I guess my samples were degrading because of some other reason...
  • Robert Göstl added an answer:
    Is it possible for a photochromic material to be both thermally reversible and photochemically reversible ?
    I am investigating a thermally reversible photochromic material, therefore, the back reaction is conducted in the dark. During normal room light conditions the forward reaction occurs readily (deep blue colour change). However, the back reaction also seems to occur by exposure to visible light (>490 nm). The back reaction is both thermally and photochemically induced.

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