- Riki Ramadian added an answer:How do you quantify fibrosis in an aorta wall with imageJ?I want to quantify fibrosis in an aorta wall with ImageJ but it seem difficult to analyze. I read there is journal to use polarization microscope, but in my laboratory there is no polarization microscope. Can ImageJ make images become polarization microscope?to adjust threshold,,,Following
- Srinath Gopalan asked a question:Matlab error!!! Can anyone explain superimposing a foreground and background image in a Watershed using Matlab..It states as Unexpected matlab command or images not of the same size... pls clarify me with exampleFollowing
- Abhijeet Lale added an answer:Is there any optical properties difference between Graphene quantum dots and N-doped graphene quantum dots?Actually, graphene quantum dots (GQD) show different optical properties. Is there any change in the optical property after doping with nitrogen in the GQD?Yes there is difference between optical properties of undoped GQD and N-doped GQD. The undoped GQD has a very high fluorescence while doped GQD have no detectable photo-luminescence. Please refer to paper by Q Li published in JACS. The following explanation is taken from the same.
This is consistent with the absence of n→π* , the lowest-energy transitions, in
the absorption spectra of doped GQD. The n→π* transitions are expected for the N-substituted aromatic heterocycles. The large size of doped GQD explains the diminishing oscillator strengths of the n→π* transitions, because of the decreasing orbital overlap between the increasingly delocalized π* electrons and the nonbonding electrons localized on the nitrogen atoms. In contrast, the lowest-energy transition in undoped GQD is π→π*, which is responsible for the emission and has a less size-dependent oscillator strength.Following
- Arunas Ramanavicius added an answer:Any suggestions on resolving the "blinking" of quantum dots (CdSe/ZnS) for confocal microscopy?I am trying to image the distribution of quantum dots (QDs) in tissue using confocal laser scanning microscopy. I am having a hard time tracking them and it is well known that the blinking of QDs poses a problem in bioimaging. Does anyone have suggestions to resolve this issue?You can encapsulate them within polymeric layr, one example is attache:Following
- Sankalp Verma added an answer:How can we prevent photobleaching in fluorescent labeled protein stock solution?My protein samples are labeled and are kept in dark at -80. I am afraid they might undergo photobleaching if kept for long.thanks...I guess my samples were degrading because of some other reason...Following
- Robert Göstl added an answer:Is it possible for a photochromic material to be both thermally reversible and photochemically reversible ?I am investigating a thermally reversible photochromic material, therefore, the back reaction is conducted in the dark. During normal room light conditions the forward reaction occurs readily (deep blue colour change). However, the back reaction also seems to occur by exposure to visible light (>490 nm). The back reaction is both thermally and photochemically induced.Following