- Yitzhak Jacobson added an answer:How can I avoid reabsorption of trace metals into solid CaCO3 during hydrogen peroxide treatment?
In preparation of aragonite shell samples for trace metals analysis, I use buffered hydrogen peroxide to remove organic matter. while this step is effective in reducing concentration of many elements, It seems that with regard to Al, Fe, Zn. Pb and perhaps a few other elements the sample becomes enriched during this process.
Thank you all for your helpful ideas.
I will try the techniques for removing the adsorbed ions, and will try to use a week acid leach as well, after the H2O2 step.
After a talk with Steve Weiner I realized that I will not be able to separate the intra-crystaline organic matter. However, the trace elements signal from this part seems stable, so I am not that worried about it. mainly concerned about remains of soft tissue and algae inside and between shells.
Thank you all againFollowing
- Noa Lincoln added an answer:How can I measure nutrient recycling in arboricultural systems?
Many studies claim one of the benefits of arboriculture is the increased uplift, retention, and recycling of mineral nutrients. However, quantification of this topic tends to be limited to very rudimentary techniques, such as simply quantifying the total nutrients redelivered to the soil surface through litter. I am interested to attempt to quantify the uplift and recycling of nutrients in arboricultural systems. The only way I can think to do it is to define a ratio of isotope fractionation for the tree crop (which will likely change with the ratio on isotopes in the soil), and to assess the isotope ratios in the soil and tree over time. Any thoughts, suggestions, citations, etc. along this topic?
Thank you for your extensive response. For the initial project I am working on young basalt soils in Kona, Hawaii. They likely have a decent amount of tephra contribution to the fines portion of the soils, with also contributions from weathering of primary material. These soils have been cropped in arboriculture in pre-European times, maintained but altered after European contact, and largely fallow in the last 50 years.
Yes the organics are a critical part of the equation, especially in the young soils as the carbon-alluminum form the majority of the nutrient retention and cation exchange capacity. One part I am interested in is how the arboricultural system might enhance the capture of weathing products, and assuming a fractionation of isotopes by plant uptake I think that the looking at the isotopes on the exchangeable nutrient sites (on carbon and alluminum oxyhydroxide) might indicate some of that. If possible in these rocky soils Iʻd also like to analyze the seepage to see what is being lost (what percentage of the loss is primary weathering and what loss is recycled nutrients that have passed through the trees).
One final bit would be about the understory species, and looking at what percentage of their nutrients are from primary weathering and what percentage have been already uptaken by the canopy trees. In this sense Iʻm interested in how the trees enable the production of the understory species.
Any citations or references to some applications of the above methods you talked about would be most helpful. Or more thoughts would also be most welcome. Thank you!Following
- Tao Jiang added an answer:How would one determine the spectral slope for dissolved organic carbon at a given wavelength range?How can I determine the spectral slope for DOM quality using UV-VIS spec data? Most literatures just mention the use of a standard equation method (non-linear fitting). Does the equation determine "S" at each wavelength using exponential curve for a given range (S275-S295, S290-S350 and S350-S400 nm) or can one use some software to determine the spectral slope at each wavelength?
Here is an important quesiton: How to decide the reasonable reference wavelenght, (lambda r)? Because currently a lot of literatures reported by using Helms et al(2008), but they didn't give the reference wavelength values specifically, this is why it's hard to results comparison.Following
- Ahmad Solgi added an answer:Any better methods to remove carbonate from soils?
I am working on some alkaline soils which have high inorganic carbonates. Yet I need to analyze the soil C content, microbial biomass C, and the 13C isotopic signature of both. Are there any classical methods for removing those inorganic carbonates? In doing so I can get clear results after the carbonate removal.
Thanks very much.
you can read these papers: 1) Canadian Journal of Soil Science, 2011, 91(2): 247-250, 10.4141/cjss10066"
Also, you read a paper and a book that I attached to you.
- Clinton Rissmann added an answer:What controls the reserves of base cations and the concentration of Ca, Mg, K and P in soil solution?
Hi hoping for people’s thoughts/comments on the generalised relationship between soil horizons (sensu lato soil depth) and the concentration of exchangeable bases as well as the regulation of inorganic ion concentrations (esp. K+ and P species) in soil solution? Specifically, is it fair to say that: (i) reserves of Ca, Mg, K and P species are highest within the subsoil due to a greater abundance of layer silicates and sesquioxides, and; (ii) that the greater abundance of layers silicates and sesquioxides results in stronger/greater regulation of the concentration or these ions in soil solution (buffering?) I realise that organic matter has by far the highest charge density (pH dependent) and yet I did not think it was particularly effective at regulating/retaining K, P or base cations relative to clays. I’m thinking particularly of fine texture (leossial) soils developed a temperate-humid climate. Clint
All, thank you for your replies. Some more thinking for comment… One of the main reasons I ask this question is for an assessment of water (ground and surface) chemistry/quality. Specifically, waters I have collected the top soils (Teflon suction cups), interflow, subsoil drainage, and overland flow (soil zone) show much higher concentrations of K, SO4, DOC, NH4, and E.coli counts but low Si concentrations when contrast with groundwater and groundwater derived baseflow. Groundwaters show tightly regulated K concentrations, much lower and less variable DOC and SO4 no E.coli and elevated Si. This all makes sense to me in terms of the vertical filtering of DOC and selective retention of and regulation of ions as waters percolate deeper into the soil and unsaturated zone before reaching the underlying aquifer. In other words waters reaching the underlying aquifer have been subject to more regulation (interacted with far greater volumes of fine textured materials) than waters derived from shallow (upper 600 mm) lateral movement of water. So although concentrations of ‘nutrients’ (K, SO4, TAM, P etc) may be higher within the upper 600 mm they do not appear to be as strongly regulated by ion exchange or sorption processes. It would therefore appear that buffering (regulation) of ‘nutrients’ is proportionately greater within the subsoil and unsaturated zone than within the upper 600 mm of the soil profile.. not necessarily the concentration of ‘nutrients’. To me this suggest either that organic matter is poor at regulating ion concentrations and or at retaining ions or that the shear abundance of nutrients from intensive land use overwhelm the regulatory (buffering) capacity of the upper portion of the soil. Regards, Clint.Following
- Eucharia O Nwaichi added an answer:How does one grind leaf samples to a fine powder for analysis by ICP-MS?I am struggling to grind my samples to a fine powder, possibly because they have a very high sap/resin content. The samples have been freeze dried under vacuum, which should make them brittle but they are not!
So far I have tried:
- a Zirconium Oxide ball mill;
- Agate mortar and pestle and liquid nitrogen;
- inside an Orbital shaker in seal jars with glass balls;
- An Agate Mill mortar and pestle mill.
I can't proceed with the analysis until these samples are powdered, and I am running out of ideas and would appreciate any suggestions from people who have successfully powdered their samples.
Dear Miranda, I will like to know if this is for elemental analysis?Following
- Awad Galal Osman added an answer:What is the optimum weight of carrier based inoculum to apply and the frequency of application for cleaning 1ha soil contaminated with 4-5% crude oil?
The carrier is charcoal. It contains a consortium of efficient bacteria (10 to power 8-9 CFU/g). The soil was contaminated 7 years ago. The soil is clay loam, pH 8.2, low N, P and organic matter content.
From literature I found that the optimum weight of the carrier based inoculum is 2% from soil weight in a pot experiment and the frequency of application was once for 150 days and the degradation% of TPH was satisfactory.Following
- Abhijit Mitra added an answer:How to estimate the population variance part (lambda) of the MSEP decomposition?
I am working on identify which explanatory variables could be interesting to add in a mechanistic model on soil carbon dynamic.
I am able to calibrate a simple model on experimental data from several sites. This model is like an average model without explanatory variables and so don't simulate the variability existing between the different sites. I have some informations about the different sites (soil properties) which could improve the predictive quality of my model.
I can estimate the MSEP of the "average" model and I'd like to estimate the population part (lambda) of the MSEP decomposition according to Bunke and Droge (1984) or Wallach and Goffinet (1987). This part represent the minimum MSEP we can get with the explanatory variables present in the model. The bigger this part is (relatively to the MSEP) the most we have to add explanatory variables to improve the predictive quality of the model. This term depends on how much the predicted variable (y) varies for fixed values of the explanatory variables (X) in the model : lambda=E[var(y|X)].
I found that when the explanatory variables are categorial, we can estimate lambda by the mean square error of the residuals of a linear model between y and X which seems logical for me. I first thought that we can do it the same way with continuous explanatory variables but I doubt now because of the linear hypothesis which can be a contribution of the squared biais part of the MSEP decomposition (Delta).
Have you any suggestions of how I estimate the lambda part of the MSEP decomposition?
Thanks for the help!
It will be better if you just tell the specific objectives and your target.Following
- Paul A Macklin added an answer:Are there scientific equipment distributors near or in Bali?
I will be conducting one year groundwater/CO2 flux research in Bali starting in a couple of months. Do you know of any suppliers for scientific equipment such as pH cal solutions/DIC vials/Hg or CO2 calibration gases? Any help would be appreciated. I am in the process of gaining a Research Visa. Best regards. Paul
Thank you so much Ari and Andre.......very helpful information for me
All the best ,
- Yash Gupte added an answer:I’m planning to investigate Al and Si co-deposition in silicophytoliths. Can anyone send me information about this subject?
I am especially interested on papers about elemental anlaysis of silicophytoliths
diatoms & scanning electron microscope with AAS, dats all u needFollowing
- Alan P Newman added an answer:Anyone familiar with Henry's constant of CO2 at different pH?
I am trying to estimate the total amount of CO2 evolution from an abiotic reaction at pH 2. I measured the headspace concentration, and am trying to calculate the aqueous concentration using Henry's constant, which is 1.1 at 20C (dimensionless). Is this number still valid at pH 2? Does the Henry's constant of CO2 change upon pH change?
Intuitively, yes. We usually strip out dissolved CO2 by acidification, and collect it using NaOH. However, it is very hard to find a table of Henry's constant of CO2 at different pH.
Actually the obvious way to determine the Henry's law constant would be at a pH which suppresses the ionisation of the hydrated carbon dioxide. If you look at the equilibrium diagrams this would be at pH 4.5 or less.
This is a matter of the formal definition of the constant which considers only gas phase carbon dioxide and the solvated carbon dioxide molecule.
By way of a practical application of this. The UK used to have a standard method for measuring sulphur dioxide in air based on an acid base titration of a bubbler solution.
The pH of this solution was set to 4.5 at the start of the experiment so as to prevent interference from carbon dioxide. Obviously sulphur dioxide also dissolves to form a weak acid but this gas was oxidised as soon as it dissolved because the bubbler solution contained hydrogen peroxide and thus sulphuric acid, a strong acid, was formed.
At the pH you are working at you should find that the literature value of Henry's law constant would predict very closely the distribution of gas phase and dissolved carbon dioxide.
Hope this helps.Following
- Paul R Bartholomew added an answer:What is this mineral as depicted in the Raman spectrum below?
We measured this Raman spectrum on a secondary mineral speleothem of a lava tube (https://www.researchgate.net/publication/265952424_RAMAN_STUDY_OF_SECONDARY_MINERALS_IN_A_RECENT_LAVA_TUBE). The main body was Thenardite and additionaly this spectrum appears occasionaly. Do you know it ?
Thanks for your help.Following
- Mikhail I Makarov added an answer:Why does the intensive soil frost increase the nitrogen concentration in leaves?
I recently found in a peatland that the plants (both mosses and sedges) that experienced more intensive winter frost (colder soil with deeper frost) for more than 10 years have a higher N concentration in the leaves or capitula. Usually, a decrease in the plant N uptake is suggested after enhanced soil frost by many studies. Can anyone provide me some reference that is in line with my finding or provide me some possible explanations? Thank you!
Concentration of N-NH4+ increases in the soil after its freezing. The mechanism of this phenomenon is other question.Following
- Kenneth M Towe added an answer:Is there anyone with research experience and interest in investigation of biogenic calcium carbonate polymorphs?
I am looking for someone, who is expert and interested in investigation of caco3 polymorphs formation through microbial activity, for cooperation in writing a research paper (as an co-author). I have the results of SEM, XRD, FTIR and light microscopy of microbially induced caco3 precipitates which I did for some other reasons than morphology. I need the expert person for interpretation of the data in view point of a specialist.Following
- Nancy Falxa Sonti added an answer:Are there any studies that quantify nutrient removal from native woody plants in stormwater management systems?
I know a group that is trying to get credit for planting native woody species in storm water management features because logically this should remove more nutrients but they need a study to provide evidence in order to get TMDL credit. Please let me know if there are any studies they can cite. Thanks!
This citation may be useful as well -
- Asmeret Asefaw Berhe added an answer:How do I measure the enzymatic activity inside and on the surface of soil aggregates?
I want to separately value enzymatic activity within the soil aggregate and on the surface of it. I can not find literature on this topic. Thank you.
I agree with the suggestions given above. I would just like to add that you do not want to use water to separate your aggregates of different sizes. Water based separation is obviously the easiest esp when you are working with small aggregates. But the rewetting and drying will very likely change the enzyme activity, and microbial community composition and abundance significantly, etc and your data will really not be useful. We recently reviewed the effect of rewetting and drying during routine soil lab analyses and the findings are pretty serious ... these procedures introduce a lot of bias and/or errors to experimental results. see the attached fileFollowing
- Antler Gilad added an answer:How important is iron and sulfur in the ecosystems you study?I just came across a paper that is very interesting discussing iron - sulfur dynamics in the presence of iron reducing bacteria. In the Everglades, the system I am interested in, iron concentrations are relatively low (generally) while in some areas sulfur (as sulfate) is extremely high.
low iron in surface water is more likely due to oxidation of iron with oxygen.
In the subsurface, Fe(2+) can interact with H2S. if you can smell H2S it should be good indication for low dissolve iron(+2) concentration as this reaction is fast. (And by the smell of H2S pretty much indicate that H2S is in excess)Following
- Alexander Galushko added an answer:Why anammox and nitrification need additional electrons for the ammonium reaction?
These two processes are involved in the nitrogen cycle. I wonder why these two pathways need additional electrons !
Oxidation state of N in nitrite is +3 and N in ammonia is -3. Therefore, net change of electrons in annamox reaction is 0.
In the first step of nitrification (oxidation of ammonia to nitrite) there is no need in additional electrons. BUT there is "hidden" requirement of electrons to "prepare" oxygen for hydroxylation of ammonia. Ammonium monooxygenase does not use O from water for hydroxylamine formation it does use O from O2. For this it needs to bring O with zero oxidation state to O with -2 oxidation state. Here additional electrons are required. However, in general equation this is "hidden" by the usage O2 as electron acceptor.
What both processes require is so called reverse electron transfer: to bring electrons released at higher redox potential reaction into lower redox potential reaction. In both cases that are reactions of assimilation of CO2. That normally is achieved by ATP hydrolysis (or any other reactions) that brings up the proton motive force and makes energetically unfavorable reaction to occur.Following
- Javier Sánchez España added an answer:Can anyone recommend literature on biogeochemical characterization of artificial lakes through dissolved nutrients?
Besides the collection of macroscopic invertebrates, I also measured dissolved nutrients during the assessment of a gravel pit lake. I would like to characterize the water of the lake using this data, but I've found no reference on methodology, yet. I would be grateful if anyone could give me advice to start out. Thank you in advance.
I attach a comprehensive study which may is not so easily available (it is a chapter from a recent book by Springer).
Here you will find lots of data and highly constructive discussions on biogeochemistry, biogeochemical cycling, food webs, trophic state and nutrient fluxes in a very special type of artificial lakes - mine pit lakes. I would specially recommend Section 3.3 (Biology and Ecosystems, starting on p. 107), since it includes information from many lakes, specially from Germany.
Probably the complexity is rather different than in a gravel pit lake, but the principles and scientific background behind their biogeochemical characterization (including the influence of dissolved nutrients on lake ecology) are the same, and thus they could be equally useful for you.
I hope this can help,
- Pete Manning added an answer:Why was the litter decomposition rate positively correlated with N content in the early stages of litter decomposition?
Many studies found that plant litter decomposition rate is related with litter concentration of nutrients ,such as N. But there is no a clear reason. I want to ask how the N concentration of litter influence on the litter decomposition.
Because nitrogen lmits microbial growth.Following
- A. R. Karbassi added an answer:Can someone suggest a review about "pore water chemistry" in marine/lake core sampling?
Especially, biogeochemistry or hydrogeochemistry in pore water of marine core deposite!
You may see the following links:-
- Clinton Rissmann added an answer:Why is the ratio of aqueous sulfate to chloride in aquatic systems related to sulfate reduction?
I am trying to understand what mechanism(s) account for the fact that the [SO42-]:[Cl-] ratio in water can be indicative of the level of activity of sulfate-reducing bacteria in aquatic ecosystems.
Hi Meredith, I relate to C. Brannon Andersen's answer. Having some understanding of the [Cl] would be very useful. If there is data from the lake from other studies you could compile an average or median [Cl]. Here in New Zealand we see considerable variation in freshwater Cl concentrations depending on the distance from the coast. However, under stable conditions the Cl concentration is usually very consistent for a given lake. With respects to redox assignments I use a field portable colorimeter to measure Total Sulfide (TS), a handheld meter for D.O., Eh, SpC, pH as well as taking samples for the main terminal electron accepting species (TEAS: NO3, Mn, Fe, SO4). If you were interested the redox assignment workbook of Jurgen et al. (USGS) is useful. Measuring total sulfide helps to discriminate as to whether FeIII or SO4 reduction is the dominant terminal electron accepting process. Kind regards, ClintFollowing
- Kenneth M Towe added an answer:Are there cost-effective ways of capturing and harvesting 'biogenic' combustible gases from seeps in the seafloor?
In the current 'climate change' syndrome, we are constantly looking for ways of ridding the atmosphere of accumulating radiative gases (so-called 'greenhouse gases, GHGs': H2O, CO2, and CH4). Over the last 3 decades, we have found thousands of locations in the seafloor, where one of the strongest GHGs is escaping, namely methane, CH4. These are active gas vents, or seeps, which are easily tracked acoustically, their manifestations known as mid-water gas plumes, or just 'flares'. I have recently found a way of harvesting such gas seepage, with a device (or system) called SUMECO: "submarine methane collector" (see my latest written contribution, in my profile). I belive this to be a viable and cost-effective way of harvesting methane from the seafloor, for use as a resource.Following
- Pascal Badiou added an answer:What is the best way to quantify the phosphorus contribution by in-lake biogeochemical cycling?
We know the internal P loading is a big contributor to algal bloom for many eutrophic lakes. But there is a lack of studies that can identify and quantify the amount of P from sediment resuspension in a eutrophic lake. Any idea or suggestion?
Another potential for examining this issue is to look at the maximum phosphorus sorption capacity of the sediments as well as the equilibrium phosphorus concentration. These will allow you to determine if the sediments in your system still have the capacity to bind P and at what water column P concentration your sediment acts as a sink or source of P. This will only identify the P fraction that is being released or taken up through biogeochemical interactions and will not provide information on P resuspension.
In terms of P resuspension there are a number of studies that have examined the impacts of benthivorous fish and wind driven sediment resuspension on TP in the water column.Following
- Amokrane Athmane added an answer:Are there any national-scale (US) land-use regression models for CO or SO2 that are publicly available?
I'm thinking of something along these lines:
But for SO2 or CO
Thanks in advance LUR experts!
Not to my knowledge, and also this is not my area of research, mine is soil parameter.Following
- John R. Helms added an answer:Does filtration of sea water result in loss of dissolved gaseous mercury (DGM) species (Hg0 and DMHg)?
Measurements of dissolved gases (oxygen, CFCs,..) in sea water samples is usually done on unfiltered samples, and tedious protocols need to be followed to avoid any bias (must be first sample to be taken, quickly after collection, no bubbles, overflow bottles 3x volume,...). DGM measurements are done on both, filtered and unfiltered samples. Could filtration result in any loss of Hg(0) or dimethylmercury?
The issue isn't filtration per say as of course they are not particles. It's the sparing solubility and significant vapor pressure of the analytes. Under vacuum filtration conditions they can partition into the headspace and be evacuated by the vacuum system. That's why using capsule filters under positive pressure (or esp. gentle gravity pressure with no pump cavitation to generate bubbles) is recommended, *if filtration is needed*. If measurements are made immediately, there is no reason to filter. If samples are to be stored, they need to be preserved in a way that preserves the Hg speciation somehow and sometimes filtration is used to remove microbes.Following
- Alexandre Castagna added an answer:Which method(s) can I use to smooth stacked curves of oxygen isotope?
I want to use Cramer et al. (2009) raw data of oxygen and carbon isotopes for a very specific time period (around 2 Ma long) to compare it to my oxygen and carbon isotopes from terrestrial record. My aim is to show that my site is affectd by global factors by comparing Cramer's data with mine. I don't have the same sample resolution as Cramer's: he has about 1000 data points while I have about 300. How can I solve the problem of difference in data resolution? Which methods would you suggest to address this issue?
I also would like to statistically compare my data with Cramer's, but my data is terrestrial so the oxygen isotope values are much lower (around -7 per mil). I would like to be able to compare the trends, not the actual values. Would anyone know which test(s) I could use?
Very nice Julie,
I would suggest to you R as very flexible and powerful tool and you can always count on its large community of users to help you out. There are a number of e-mail groups dedicated to specific research areas with R. Good luck with your research.Following
- Michel Vorenhout added an answer:How can I make an anaerobic environment in a 250 ml tight bottle contain Soil:water(1:1) to get methane ?
Degradation of carbon at a certain temperature and humidity evolve methane. Only methods are require to maintain anaerobic Environment in small bottle.
@Jean-Rene: new question?Following
- Per Hagelia added an answer:Can anyone recommend updated solubility constants for minerals associated with ARD?
I basically need access to reliable solubility constants of sulfates which are associated with acid rock drainage, acid sulfate soils and acid mine drainage.
Perhaps someone has made a recent compilation?
Thank you both for swift response.Following
- Harald G. Dill added an answer:Dear fellows, Do you know any comprehensive review about soil weathering indexes?
Weathering indexes are useful to estimate the intensity or the duration of a weathering process in geological materials as soils. But what are the pros and cons? What are the methodological issues about these indexes? And, finally, why aren't they popular? For soils there are two interesting indexes: the Ki (Silicate to Al-oxides ratio) and the Kr (Silicate to oxides ratio). In simple words: How intenser and/or longer the weathering process, more intense the loss of silicates will be and more oxides will remain in the soil. Therefore, higher the Kr or Ki ratio. As a rule of thumb, soils with Ki < 2 (devoid of 2:1 phyllosilicates) are the highly weathered ones. The highly weathered soils from Brazil very often presents Ki and Kr < 1.
Dear Mr. Sobrinho,
the approach you will have to take, strongly depends on the mineral assemblage of your weathering profile. I have used clay minerals and heavy minerals. The latter group is rather sensitive and even can show you the intensity by the crystal morphology (see file attached). In your environment, newly formed APS minerals may also be of help (Aluminium-Phosphat-Sulfate minerals/ crandallite group), which can be used in combination with sequioxides (Al oxides) and phyllosilicates (kandite-group + halloysite). The following paper may give you an introduction into the matter
DILL, H.G. (1998) A review of heavy minerals in clastic sediments with case studies from the alluvial fan through the near-shore marine environments.- Earth Science Review, 45: 103-132.
DILL, H.G. (1995) Heavy mineral response to the progradation of an alluvial fan: implications concerning unroofing of source area, chemical weathering, and paleo-relief (Upper Cretaceous Parkstein fan complex / SE Germany).- Sedimentary Geology, 95: 39-56
DILL, H.G. (2001) The geology of aluminium phosphates and sulphates of the alunite supergoup: A review.- Earth Science Reviews, 53: 35-93.
Should you have further questions feel free to contact me on this matter.
Harald G. DillFollowing
Biogeochemistry is the scientific discipline that involves the study of the chemical, physical, geological, and biological processes and reactions that govern the composition of the natural environment (including the biosphere, the hydrosphere, the pedosphere, the atmosphere, and the lithosphere).