This group is designed for the exchange of thoughts on Biochemistry

• Swarnali Dutta Majumdar

  What could be potential natural antioxidants to prevent and/or retard citrus oil oxidation?

  The extracted peel oil from citrus fruits are highly susceptible to oxidation. Can anyone suggest me some natural antioxidant that can retard the oxidation of these citrus oils? If you can share someThe extracted peel oil from citrus fruits are highly susceptible to oxidation. Can anyone suggest me some natural antioxidant that can retard the oxidation of these citrus oils? If you can share some published paper or any concrete resource like this, then it will be really helpful.

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  • 
    Andri Kurniawan replied

    But, im ever tried ascorbat acid.

    10 hours ago
• Ying Tang

  Does rat liver HDAC lack substrate specificity?

  I'm screening HDAC's inhibitors, but have met the challenge of the substrate specificities of different HDACs. I changed the classic Boc-Lys(Ac)-MCA substrate to a long peptide likeI'm screening HDAC's inhibitors, but have met the challenge of the substrate specificities of different HDACs. I changed the classic Boc-Lys(Ac)-MCA substrate to a long peptide like CGGAFFGGK(Ac)-MCA,CGGAFFLYK(Ac)-PEG, is this still suitable for class I HDAC deacetylation? I reviewed some articles and find that most of them use rat liver to catalyze the deacetylation of classic substrate, I'm wondering maybe my long peptide substrates may suitable rat liver HDAC deacetylation.

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  • 
    Ying Tang replied

    Thank you so much!

    2 days ago
• Raktim Biswas

  How to determine ATP spectrophotometrically?

  Is it possible to determine ATP in mice tissue by spectrophotometer? Mostly ATP is determined by luminescence, but I am looking for a method to determine ATP spectrophotometrically.

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  • 
    Raktim Biswas replied

    I'll surely do so...Thank you all for your support...

    3 days ago
• Kamala Katepogu

  Is there specific programmable freezer equipment for cryopreservation?

  How does it work?

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  • 
    Kamala Katepogu replied

    Sir, how much it's cost? How it works?

    3 days ago
• Yong Kuen Ho

  Oligosaccharides assaying using thin layer chromatography

  Does anybody know whether it is possible to quantify the amount of different oligosaccharides present in a mixture by using thin layer chromatography? Or is there any other cheaper and faster methodDoes anybody know whether it is possible to quantify the amount of different oligosaccharides present in a mixture by using thin layer chromatography? Or is there any other cheaper and faster method of oligosaccharides assaying as I do not want to use the HPLC due to the laborious effort and time involved.

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    Alessandra Morana replied

    I do not suggest TLC to quantify oligosaccharides. You can have just an idea of which oligo is more abundant than others. HPLC with PAD (pulsed amperometric detector) can be the best choice. ThisI do not suggest TLC to quantify oligosaccharides. You can have just an idea of which oligo is more abundant than others. HPLC with PAD (pulsed amperometric detector) can be the best choice. This detector is very sensitive and it borns for sugar quantification. It is more sensitive than refractive index.

    4 days ago
• Yong Kuen Ho

  Is amylase production in microbes growth associated?

  Does anybody know whether the production of alpha-amylase, beta-amylase and glucoamylase from bacterial and fungal species is growth or non-growth associated?

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  • 
    Ullrich Keller replied

    Expression of amylase genes is inducible by the presence of starch. Most pobably, the effect itself is exerted by small molecules (Maltose or isomaltose) resulting from breakdown of starch catalysedExpression of amylase genes is inducible by the presence of starch. Most pobably, the effect itself is exerted by small molecules (Maltose or isomaltose) resulting from breakdown of starch catalysed by low constitutive presence of amylase. The expression of amylase in firmicutes is subject to repression by glucose. Therefore, amylase production is not 'growth-related' or 'constitutive', but inducible. Nevertheless, one cannot say that amylase is a secondary enzyme because it plays a major role in catabolism when necessary. Promoters of various amylase genes have been shown to have similarity to sigma70 consensus of E. coli or to the veg promoter of B. subtilis which indicates that they are house-keeping genes. However, regulatory sequences (inverted or directed repeats) in the promoter upstream regions or overlapping with the -35 box are binding sites for repressors (such as MalR) which keep these promoters silent as long as gluose is present (or maltose absent)

    5 days ago
• Prem Prakash

  What will happen if one mixes solutions of different pHs?

  What will happen with the resultng pH if I mix a solution of pH 5 and a soution of pH 8 or any other higher pH together? Will there be any effect on mixing more than two solution with different pH?

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  • 
    Aleksandar Djordjevic replied

    First, it is important that the chemicals found in the solutions. It can be difficult to form precipitate, complexes, gases. If the salt is formed which does not hydrolyse (NaCl) is essential to howFirst, it is important that the chemicals found in the solutions. It can be difficult to form precipitate, complexes, gases. If the salt is formed which does not hydrolyse (NaCl) is essential to how many volumes are mixed to calculate the final pH.

    6 days ago
• Olakunle Ogunyemi

  Please can anyone tell the method to assay for LEPTIN or where i can buy it I need it for my MSc project. "The therapeutic effect s of leptin on glucose metabolism and diabetes complications"

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  • 
    Olakunle Ogunyemi replied

    @Gustavo, thanks very much, but what i could deduce here is how to purchase it. Please is there no method to prepare it in the lab? Thanks again

    6 days ago
• Aneesh P. A

  How to isolate and purify a particular protein band from PAGE gel?

  I have done PAGE of fish protein. nowI want to select and cut out a particular band from the gel and purify it. please inform me what are the protocols for purifying it from the gel material.

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  • 
    Raghavendra Sathyanarayana replied

    Do native page then the protein can be separated easily

    12 days ago
• Gunjan Pandey

  Can anyone explain in detail the difference between Ferric Citrate and Ferric Ammonium Citrate?

  I was able to find that Ferric citrate provides iron in less toxic form than other free iron salts but was not able to find any detail on either how such mechanism is possible.
• Gaurav Bhardwaj

  What are different tools for gene annotation except David and Panther?

  Want to know the different Go-Annotation tools?

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  • 
    Rajashri Bhairamadgi replied

    GATHER: Gene Annotation Tool to Help Explain Relationships http://gather.genome.duke.edu/ try out may help

    13 days ago
• Yong Kuen Ho

  Quantifying fungal growth

  I have a mixture of different fungi's (including different yeast strains) in a submerged fermentation broth. How can I quantify the total growth of these fungis without discriminating between theI have a mixture of different fungi's (including different yeast strains) in a submerged fermentation broth. How can I quantify the total growth of these fungis without discriminating between the individual species? I heard that the glucosamine method is quite laborious. Is there any better and faster method?

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  • 
    Christopher Burlak replied

    http://www.cas.muohio.edu/botany/people/profiles/Money.html Yafetto, L., Davis, D. J., and Money, N. P. 2009. Biomechanics of invasive growth by Armillaria rhizomorphs. Fungal Genetics and Biologyhttp://www.cas.muohio.edu/botany/people/profiles/Money.html Yafetto, L., Davis, D. J., and Money, N. P. 2009. Biomechanics of invasive growth by Armillaria rhizomorphs. Fungal Genetics and Biology 46: 688-694. doi:10.1016/j.fgb.2009.04.005 Money, N. P. 2008. Insights on the mechanics of hyphal growth. Fungal Biology Reviews 22: 71-76. Money, N. P. 2007. Biomechanics of invasive hyphal growth. In: Howard, R. J., and Gow, N. A. R., eds. The Mycota, Volume 8, Biology of the Fungal Cell, 2nd edition. Springer Verlag, New York, pp. 237-249. Good Luck.

    14 days ago
• Rajneet Khurana

  17-a-(2-acetoxy ethyl)-3 beta- (1-piperdino) 17-a aza- D- homo-5- androsten dimethiodide

  Why does 17-a-(2-acetoxy ethyl)-3 beta- (1-piperdino) 17-a aza- D- homo-5- androsten dimethiodide turn dark on heating?
• Christopher Burlak

  Is alpha enolase glycosylated in pigs and/or humans?

  I have seen mixed data in publications and found it to be non-glycosylated by lectin immunoblot and Schiffs Base reagent. Does anyone have a different experience? Thanks in advance for your comment.
• Pankaj Paliwal

  How insulin can labelled by any method other than radio labeling, so that it can be estimated and distinguished from body insulin?

  How insulin given artificially can be separately estimated so that to have clear idea weather the change in glucose level is only because of insulin admins-tired or body insulin?

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  • 
    Aritra Chowdhury replied

    Flourescence!!Label it with a flourophore like FITC.

    16 days ago