Biochemical Engineering

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Question:
1 Can anybody suggest me the concentration of ammonia needed to be used for pH control in fed batch fermentation?
I am working on production of acid metabolites in E.coli in which I have to compare the effect of various bases like NaOH, KOH and Ammonia etc. Could ... [more]

I am working on production of acid metabolites in E.coli in which I have to compare the effect of various bases like NaOH, KOH and Ammonia etc. Could anybody please suggest the concentration of ammonia for neutralization? Thanks in advance.

By Mukesh Sankar · Pusan National University

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Question:
12 How can I design an MBBR system?
I want to design an MBBR (moving bed biological reactor), and I need design parameters. Is there any book, article or etc. about MBBR's design?

I want to design an MBBR (moving bed biological reactor), and I need design parameters. Is there any book, article or etc. about MBBR's design?

By Maryam sinaee nejad · Khaje Nasir Toosi University of Technology

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Question:
3 How can I best promote hairpin formation for a synthesized oligonucleotide?
I am designing a substrate for a nuclease assay, and want to utilize the formation of a hairpin in an oligo to remove the tedious purification steps t... [more]

I am designing a substrate for a nuclease assay, and want to utilize the formation of a hairpin in an oligo to remove the tedious purification steps that come from annealing two strands. Currently, I have a TTTT loop that is flanked by complementary DNA that should form 16 base pairs, with about 60% GC content. Does anyone out there use a similar substrate and have some wisdom to share? I am mostly concerned with how high a concentration I can get away with during annealing, and the best salts & salt concentrations to use. I am currently annealing at 2uM in TE with 100 mM KCl.

By Elizabeth Mcsweeney · Duke Medicine

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Question:
7 Calculation of solubility using predictive or another activity coefficient models?
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By Behnaz Asadzadeh · University of Tabriz

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Question:
4 Anaerobic Digestion Model ADM1?
Can anyone give me some typical values for total inorganic carbon (TIC mol HCO3/m3) and for alkalinity (cations Scat eq/m3) to use as input for activa... [more]

Can anyone give me some typical values for total inorganic carbon (TIC mol HCO3/m3) and for alkalinity (cations Scat eq/m3) to use as input for activated sludge anaerobic digestion modelling with ADM1?

By Andrea Gianico · National Research Council

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Question:
110 Is the relation between OD reading and cell concentration (cells/mL) of bacteria different in various culture media?
For instance, assume that the cell concentration of S.pasteurii cultivated in an Ammonium YE medium is estimated by the expression 8.59* 10^7(Z^1.363)... [more]

For instance, assume that the cell concentration of S.pasteurii cultivated in an Ammonium YE medium is estimated by the expression 8.59* 10^7(Z^1.363) where Z is the reading at OD600. Now, if I cultivate the S.pasteurii in an Acetate YE medium, is it possible to use the same equation to estimate the number of cells according to the OD readings?

By Hamed Khodadadi · Eastern Mediterranean University

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Question:
4 Amount of cellulase in lignocellulose enzymatic hydrolysis.
If the cellulase determine at 67 FPU/ml, what is the amount of cellulase (?? FPU/ml: 20, 40?) needed to be used in 1% biomass enzymatic hydrolysis?

If the cellulase determine at 67 FPU/ml, what is the amount of cellulase (?? FPU/ml: 20, 40?) needed to be used in 1% biomass enzymatic hydrolysis?

By Jacky Lai · UNISEL | Universiti Selangor

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Question:
6 Does the distance between RBS and ATG (initiation codon) effect protein expression?
I constructed a plasmid DNA part through standard BioBrickTM assembly and sent for sequencing. The sequencing result is a little different than with o... [more]

I constructed a plasmid DNA part through standard BioBrickTM assembly and sent for sequencing. The sequencing result is a little different than with other previous parts I have constructed. The number of bases between RBS and ATG is 8, other than 6. I am wondering if there will be any effect on downstream expression?

By Sophia Wang · Xiamen University

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Question:
Open What is the best media composition for optimal growth of Rhodococcus opaqus?
it should be specific for strain PD630 which is used for accumulation of TAG

it should be specific for strain PD630 which is used for accumulation of TAG

By Sanjay Kumar · Indian Institute of Technology Guwahati

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Question:
1 How do I perform a rapid, cellobiase activity determination assay?
I am performing the cellulase + cellobiase activity checking. For cellulase, I managed to check its activity in FPU/ml as described by Ghose, 1987. ... [more]

I am performing the cellulase + cellobiase activity checking. For cellulase, I managed to check its activity in FPU/ml as described by Ghose, 1987. Can anyone tell me the rapid, simple method to check the cellobiase activity? Any paper to refer?

By Jacky Lai · UNISEL | Universiti Selangor

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Question:
2 Is NaCl an obstacle to solubilize proteins from Inclusion bodies?
I'm trying to dissolve the inclusion body that contains rPA but my results are not good. For instance I've used a buffer consisted of 4 M Urea, 50mM ... [more]

I'm trying to dissolve the inclusion body that contains rPA but my results are not good. For instance I've used a buffer consisted of 4 M Urea, 50mM 2-mercaptoethanol , 200 mM NaCl , 1mM EDTA and 50mM Tris base. The pH of the buffer is 9 but the final concentration of protein is 0.7 mg/ml. I've read different papers and I think this buffer should be able to solubilize any protein. I saw a paper that argues that NaCl may lead to salting out of soluble rPA. Could it be NaCl that prevents solubilization?

By Sajjad Naeimipoor · Tarbiat Modares University

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Question:
15 What is the best method to gain Hydrogen For Seasonal Energy Storage?
Hydrogen gas as fuel is used nowadays for many purposes. So which method is best to gain Hydrogen for its storage and which one gives maximum efficien... [more]

Hydrogen gas as fuel is used nowadays for many purposes. So which method is best to gain Hydrogen for its storage and which one gives maximum efficiency?

By Muhammad Sajid Qayyum · University of Gujrat

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Question:
7 I want to draw a growth curve for Pichia pastoris X33 on defined glucose minimal medium. Can anybody give me details about medium preparation?
I'm using this medium: (Chung et al. 2010) (per liter): 50 g glucose, 1.5 g MgSO4·7H2O, 0.2 mg Biotin, 3 g KH2PO4, 15 g (NH4)2SO4, 2.0 mL of trace sa... [more]

I'm using this medium: (Chung et al. 2010) (per liter): 50 g glucose, 1.5 g MgSO4·7H2O, 0.2 mg Biotin, 3 g KH2PO4, 15 g (NH4)2SO4, 2.0 mL of trace salts stock solution and 1.0 mL of a solution containing 2 g/L biotin in 1M NaOH. The trace element solution contained (per liter) 5 mL H2SO4, 4.0 g CuSO4·5H2O, 14.0 g MnSO4·H2O, 2.6 g Na2MoO4·2H2O, 4.0 g H3BO3, 4.0 g CoCl2·6H2O, 22.0 g ZnSO4·7H2O, 55.0 g CaCl2·2H2O and 37.5 g FeCl3·6H2O. But there is a problem with trace solution. Addition of ZnSO4.7H2O(22 g/lit) and CaCl2.2H2O(55 g/lit) produce a solid precipitate. What should I do?

By Ali Ehsani · Iran University of Science and Technology

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Question:
17 What parameters should one consider for designing a cyclone separator for removing solids from gas?
I want to design a cyclone separator for cleaning the product gases of a steam gasification process. The gases will exit the reactor at around 850degC... [more]

I want to design a cyclone separator for cleaning the product gases of a steam gasification process. The gases will exit the reactor at around 850degC.

By Reeshab Goenka · National Institute of Technology Tiruchirappalli

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Question:
Open I am searching for information about aliphatic nitrilases. Does anyone know which microbe synthesizes nitrilase NIT-102?
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By Leopoldo González Brusi · Universidad de Murcia

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Question:
1 How two detect directional distance between two ellipsoids that have overlap with each other?
I have a control problem that involves two ellipsoids named as ROC and ROD, in my algorithm the condition of stability is as following: the ROC should... [more]

I have a control problem that involves two ellipsoids named as ROC and ROD, in my algorithm the condition of stability is as following: the ROC should be in ROD, we know that ROC and ROD in the worst case have overlap with each other. We need to enlarge ROD such that ROC is in that and this should be optimized. I have some questions, as follows: 1- How to detect the distance of ellipsoids when one ellipsoid is completely in the other or when they have overlap? (except contact points that distance is zero) 2- When two ellipsoids have overlap how can I enlarge one ellipsoid such that it touches the second ellipsoid? 3- When two ellipsoids have no contact (they are not separated) how can I change the bigger ellipsoid such that it touches the inner ellipsoid?

By Saeed Dehghani · Sharif University of Technology

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Question:
Open Autoclave electricity usage calculation.
I am looking for a way to calculate / measure the autoclave electricity usage. FYI, the autoclave of mine stated 2kWatt of electric power. What is the... [more]

I am looking for a way to calculate / measure the autoclave electricity usage. FYI, the autoclave of mine stated 2kWatt of electric power. What is the total power use for 1-cycle, 3-cycle? Cycle: 1. start from room temp. 25C to 100 C, 2. pressure start increase from 0 to 1.7kg/cm2 at 100C, 3. maintain sterilization at 121C, 1.7kg/cm2 for 15 min. 4. pressure released to 0 and temp drop to 100C 5. temp further drop to 50C (keep warm status)

By Jacky Lai · UNISEL | Universiti Selangor

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Question:
Open What is the partition coefficient value of Methanol when the solute is 1.) an Amino acid and 2.) Cyclohexadiene compound
These values play an important role in the process of Secondary Metabolite extraction from Microbial and Plant cells

These values play an important role in the process of Secondary Metabolite extraction from Microbial and Plant cells

By Srivatsava Viswanadha · Indian Institute of Technology Madras

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Question:
1 Hi, kindly share some experience in measurement of celulase enzyme, FPU??
I had performed cellulase activity checking based on Ghose, T.K. (1989) protocol. The enzyme activity was determined at 121.0 FPU/ml (at 0.0030 Ezy co... [more]

I had performed cellulase activity checking based on Ghose, T.K. (1989) protocol. The enzyme activity was determined at 121.0 FPU/ml (at 0.0030 Ezy concentration). 1. What does it mean? 2. How many ml of enzyme (at 0.0030 enzyme concentration from 1:20 stock dilution) that need to add-in to degrade 5% (w/v) biomass at final volume of 50ml? Thank you.

By Jacky Lai · UNISEL | Universiti Selangor

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Question:
Open Is gasification of agricultural resources a process compatible with urban environment?
I search for publications about the gasification plant in neighborhood and suburbs.

I search for publications about the gasification plant in neighborhood and suburbs.

By Benedetto Nastasi · Sapienza University of Rome

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Question:
2 What are a few techniques to isolate manganese oxidizing/reducing bacteria?
I'm studying the biological reduction and oxidation of manganese, and for this purpose I have to apply some techniques, but the information that I hav... [more]

I'm studying the biological reduction and oxidation of manganese, and for this purpose I have to apply some techniques, but the information that I have doesn't mention these techniques or the methods of selection of the bacterias.

By Francisco Valdebenito · San Sebastian University

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Question:
13 Can anyone suggest he best adsorbent for chromium?
I am trying to convert chromium into a harmless material for making waste containing chromium suitable for direct disposal.

I am trying to convert chromium into a harmless material for making waste containing chromium suitable for direct disposal.

By Nishit Sangomla · National Institute of Technology Tiruchirappalli

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Question:
1 Does anybody have data on the current (2012) global energy demand (consumption) and production?
I'm referring to total energy consumption.

I'm referring to total energy consumption.

By Olujimi Dada · National University of Malaysia

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Question:
5 Protein determination.
I am going to perform protein determination on an oil palm biomass sample. Among the two methods, Lowry and Bradford, which one is more convenient? Ca... [more]

I am going to perform protein determination on an oil palm biomass sample. Among the two methods, Lowry and Bradford, which one is more convenient? Can anyone share their experiences?

By Jacky Lai · UNISEL | Universiti Selangor

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Question:
2 Dry processing of minerals
What factors am l supposed to consider during the design of such a plant and any additional information on links and textbooks which l can use are mos... [more]

What factors am l supposed to consider during the design of such a plant and any additional information on links and textbooks which l can use are most welcome.thanks

By Penelope Malunga · Harare Institute of Technology

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Question:
Open What is the role of biochemical markers in defining and determining the specific metabolic perturbation and dysfunction?
What is the role of biochemical markers in defining and determining the specific metabolic perturbation and dysfunction?

What is the role of biochemical markers in defining and determining the specific metabolic perturbation and dysfunction?

By Sanjay Mishra · IFTM University

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Question:
1 What are the catalyst that use for methanol reforming?
What are the catalyst that use for methanol reforming to produce hydrogen?

What are the catalyst that use for methanol reforming to produce hydrogen?

By Hjgk Hjk · Sirindhorn International Institute of Technology (SIIT)

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Question:
11 What is short communication paper?
From a scientific point of view, what is the value of short communication papers compared to review papers and research papers?

From a scientific point of view, what is the value of short communication papers compared to review papers and research papers?

By Hadi Hezaveh · University of Queensland

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Question:
1 How can I separate the cells of a tissue of extracellular matrix?
How can I separate the cells of a tissue of extracellular matrix? I would like to know the process and the percentages of compounds to be used. The ce... [more]

How can I separate the cells of a tissue of extracellular matrix? I would like to know the process and the percentages of compounds to be used. The cells will be discarded. I am interested only in extracellular matrix, not cells. Thanks.

By Toni Rodriguez · Haun

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