Research and developments in the field of bacteriophage related to its classification, Molecular biology, Bioinformatics, etc. and their biochemical and genetic interactions with bacterial communities and co-evolution.Virusoids, viriods, prions, bacteriophages and other virus like particles all have a very magnificent way of living their parasitic intracellular life and they do have a significant effect on the evolution of smallest and the largest living organism on earth.

• Ayu Liana

  I would like to share this interesting article about bacteriophage!

  http://www.hitxp.com/articles/science-technology/sacred-mystery-secrets-ganga-ganges/

  Recent replies ⋅ Show All (1)

  • 
    Imtiyaz Rather replied

    Bacteriophage is a microb but not a bacteria infact a virus, a structural configuration of a bacteriophage is a discoid body with a spiral filament the independent virlunce of hidden spore and aBacteriophage is a microb but not a bacteria infact a virus, a structural configuration of a bacteriophage is a discoid body with a spiral filament the independent virlunce of hidden spore and a viral transformation in host body is treated with antivirals; like aciylclover.The recent biomedical space research had invented bacteriophage in space culture at micro gravity.

    19 days ago
• Ayu Liana

  How to pellet bacteriophage using ultracentrifuge? is it necessary to add sucrose or CsCl to improve the separation?

  I just started culturing bacteriophage and I encounter a problem in pelleting the bacteriophage. I did a series of centrifugation at different speeds to remove bacteria debris (at low speed), and toI just started culturing bacteriophage and I encounter a problem in pelleting the bacteriophage. I did a series of centrifugation at different speeds to remove bacteria debris (at low speed), and to pellet the bacteriophage (at high speed, 13000g for 2 hr). However, I found that sometimes the bacteriophage couldn’t be pelleted, although I have followed the same procedure. I am wondering if I might have missed something here.

  Recent replies ⋅ Show All (14)

  • 
    Manikandan Mohan replied

    Dear friends, wishes and greetings I wish to express my peptide in Gene 8 fusion. Length of my peptide is in around 50 AA. Can u clear me whether large fusion could affect the phage infectivity orDear friends, wishes and greetings I wish to express my peptide in Gene 8 fusion. Length of my peptide is in around 50 AA. Can u clear me whether large fusion could affect the phage infectivity or not?. If its possible which vector i have to be choose for gene 8 fusion.

    20 days ago
• Arthur Leibovitz

  Is therapy with bacteriophages in use presently in any place?

  Recent replies ⋅ Show All (1)

  • 

    Comment protected

    Join ResearchGate now to read this comment.
• Ramanandan Prabhakaran

  Can anyone recommend a list of highly and poorly expressed genes for bacteriophages?

  If you are aware of any database that has these data please let me know? Thanks in advance

  Recent replies ⋅ Show All (5)

  • 
    Ramanandan Prabhakaran replied

    Thanks a lot for all of them for giving me the useful resources. @ Mette - I am in general looking for the phages and its respective highly and poorly expressed genes. @ Elizabeth - I am lookingThanks a lot for all of them for giving me the useful resources. @ Mette - I am in general looking for the phages and its respective highly and poorly expressed genes. @ Elizabeth - I am looking for the bacteriophages infecting bacteria. I could see the gene expression data for bacteria but not many resources for phages. @Robert- As you mentioned I am focussing on capsid and coat proteins.

    Mar 21, 2012
• Masoodur Rahman

  Can bacteria digest phages?

  Just to clear my curiosity
• Orr Shapiro

  What are the relevant time scales for phage ?

  I believe many of the questions regarding phage in the environment must involve a temporal dimension. Not just "who is there? but also "when?" and "for how long?". I therefore find the dynamics ofI believe many of the questions regarding phage in the environment must involve a temporal dimension. Not just "who is there? but also "when?" and "for how long?". I therefore find the dynamics of phage-host interactions to be of great interest. Phage time scales in the lab can be rather short. A phage infection cycle might be less than 30 min from attachment to burst, clearing a test tube with a dense bacterial culture is a matter of hours and plaques on a petri dish are often detectable overnight. However, these statistics will change in different growth conditions and different age of the infected host and are therefore not necessarily applicable outside the lab. What are the relevant time scales when working in the real world? How long does a phage "epidemic" lasts before it burns itself out? How long does it take for a phage to go from undetectable to peak abundance and back to undetectable in your environment of choice? Is this a characteristic of the environment, the specific phage-host system or both? What about lysogenic phage? Once lytic cycle is induced, how long before a new "status-quo" is established? I'll be happy to hear your experience and/or thoughts on these questions

  Recent replies ⋅ Show All (7)

  • 
    Paola Di Bonito replied

    Thank you Robert

    Feb 19, 2012
• Swapnil Ganesh Sanmukh

  Can Viruses be considered responsible factors for co-evolution of bacteria and higher organisms?

  It is well known that transduction is an important characteristics which viruses show in nature responsible for imparting various alien characteristics to their hosts. They are said to be specific,It is well known that transduction is an important characteristics which viruses show in nature responsible for imparting various alien characteristics to their hosts. They are said to be specific, but to what extent, since various viruses like phages are found to be non-specific. In such case, immune system and its components can be crucial as viruses have ability to manipulate their mechanism in differentiating self and non-self entities in the host organism. The viruses (like phages) can be the important tool for understanding genetic shuffling.

  Recent replies ⋅ Show All (13)

  • 
    Paola Di Bonito replied

    Orr , yes new science is there my point of view is from the laboratory bench, do you create mathematical models and need to imagine scenarios?

    Feb 10, 2012
• Swapnil Ganesh Sanmukh

  Can Pseudolysogeny be the initiation for Plasmid acquisition by a bacterial host cells?

  As we see most of the bacterial host have varying number of plasmid with them, which are not yet defined properly in terms of origin and occurrence, it can also be said that Pseudolysogeny induced byAs we see most of the bacterial host have varying number of plasmid with them, which are not yet defined properly in terms of origin and occurrence, it can also be said that Pseudolysogeny induced by the phages in energy deficient host may be the beginning for the acquisition of such extra-chromosomal genetic material within the bacterial host cell. Moreover, we know that most of the bacterial cells present in adverse or stressed environment fulfills the required conditions for Pseudolysogeny (as they are nutrient limiting) and have maximum number of plasmids. Existence of pro-phages in different bacterial chromosomes is well known; but they also exist in plasmids, however some plasmids as a whole can be phage genome itself , which can verifying the pseudolysogenic origin of plasmid.
• Swapnil Ganesh Sanmukh

  The pro-phages from some bacterial hosts are given attached as 3 zip files in .rar format

  3.rar
• Swapnil Ganesh Sanmukh

  The pro-phages from some bacterial hosts are given attached as 3 zip files in .rar format

  2.rar
• Swapnil Ganesh Sanmukh

  Here are some of the pro-phages detected from different microbes by the use of PHAST online server.

  The pro-phages from some bacterial hosts are given attached as 3 zip files in .rar format.

  1.rar
• Swapnil Ganesh Sanmukh

  What do you say about bacterial genetic classification without Phage (pro-phage) intervention?

  we are carrying out genetic analysis with gene sequencing and bioinformatic tools and are coming to a conclusion that "Each and every bacterial genus has some phage integrated genetic elements inwe are carrying out genetic analysis with gene sequencing and bioinformatic tools and are coming to a conclusion that "Each and every bacterial genus has some phage integrated genetic elements in intact or incomplete pro-phage form within there genome", further making bacterial genetic classification a joke in absence of phage genetics. Moreover, some plasmids too contain phage derived genetic elements again questioning their source of origin.
• Swapnil Ganesh Sanmukh

  What do you think about the role of bacteriophages in bacterial or organismic evolution

  As i am working on bacteriophages, i am comming across various references about phage regulating the vital functioning in bacteria which are also responsible for various threats for higher organismsAs i am working on bacteriophages, i am comming across various references about phage regulating the vital functioning in bacteria which are also responsible for various threats for higher organisms including us. I just want to have a broad discussionon this topic.

  Recent replies ⋅ Show All (1)

  • 
    Agung Putra Purwanto replied

    well...I'm agung putra purwanto..I'm college in brawijaya university, Indonesia..now I'm helping my lecturer to isolation of bacteriophage, hope if success later, I want to application inwell...I'm agung putra purwanto..I'm college in brawijaya university, Indonesia..now I'm helping my lecturer to isolation of bacteriophage, hope if success later, I want to application in agriculture to remove Salmonella sp.

    Jun 16, 2011